BRPI0607751A8 - organismo hospedeiro, sistema de cultura de célula, método para melhorar a produtividade da expressão de proteína dependente da vitamina k recombinante ou de um derivado desta funcionalmente ativo em um organismo hospedeiro, e, proteína dependente da vitamina k recombinante - Google Patents
organismo hospedeiro, sistema de cultura de célula, método para melhorar a produtividade da expressão de proteína dependente da vitamina k recombinante ou de um derivado desta funcionalmente ativo em um organismo hospedeiro, e, proteína dependente da vitamina k recombinanteInfo
- Publication number
- BRPI0607751A8 BRPI0607751A8 BRPI0607751A BRPI0607751A BRPI0607751A8 BR PI0607751 A8 BRPI0607751 A8 BR PI0607751A8 BR PI0607751 A BRPI0607751 A BR PI0607751A BR PI0607751 A BRPI0607751 A BR PI0607751A BR PI0607751 A8 BRPI0607751 A8 BR PI0607751A8
- Authority
- BR
- Brazil
- Prior art keywords
- host organism
- dependent protein
- recombinant
- vitamin
- productivity
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0093—Oxidoreductases (1.) acting on CH or CH2 groups (1.17)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/644—Coagulation factor IXa (3.4.21.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y117/00—Oxidoreductases acting on CH or CH2 groups (1.17)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21022—Coagulation factor IXa (3.4.21.22)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
organismo hospedeiro, sistema de cultura de célula, método para melhorar a produtividade da expressão de proteína dependente da vitamina k recombinante ou de um derivado desta funcionalmente ativo em um organismo hospedeiro, e, proteina dependente da vitamina k recombinante. a presente invenção diz respeito a um organismo hospedeiro contendo ácidos nucleicos recombinantes que codificam a subunidade 1 do complexo de vitamina k redutase (vkorc 1) e ácidos nucleicos recombinantes que codificam uma proteína dependente de vitamina k (vkd), em que tanto a vkorc1 recombinante quanto a proteína vkd recombinante são expressadas no dito organismo hospedeiro. além disso, a presente invenção diz respeito a um sistema de cultura de célula que compreende células contendo os ditos ácidos nucleicos recombinantes e aos métodos para melhorar a produtividade de expressão de proteína vkd recombinante em um organismo hospedeiro que é cultivado em sistemas adequados.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US65704105P | 2005-02-28 | 2005-02-28 | |
| US60/657041 | 2005-02-28 | ||
| PCT/EP2006/000734 WO2006089613A1 (en) | 2005-02-28 | 2006-01-27 | Recombinant co-expression of vitamin k epoxide reductase subunit 1 to improve vitamin k dependent protein expression |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BRPI0607751A2 BRPI0607751A2 (pt) | 2010-03-16 |
| BRPI0607751A8 true BRPI0607751A8 (pt) | 2015-10-27 |
Family
ID=36130202
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0607751A BRPI0607751A8 (pt) | 2005-02-28 | 2006-01-27 | organismo hospedeiro, sistema de cultura de célula, método para melhorar a produtividade da expressão de proteína dependente da vitamina k recombinante ou de um derivado desta funcionalmente ativo em um organismo hospedeiro, e, proteína dependente da vitamina k recombinante |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US9617523B2 (pt) |
| EP (2) | EP2295547B1 (pt) |
| JP (3) | JP2008531026A (pt) |
| KR (2) | KR20130025952A (pt) |
| CN (2) | CN103396998A (pt) |
| AU (1) | AU2006218216B2 (pt) |
| BR (1) | BRPI0607751A8 (pt) |
| CA (1) | CA2599284A1 (pt) |
| DK (1) | DK1853700T3 (pt) |
| ES (1) | ES2397530T3 (pt) |
| HK (1) | HK1113498A1 (pt) |
| HR (1) | HRP20130028T1 (pt) |
| IL (1) | IL184696A (pt) |
| MX (2) | MX349285B (pt) |
| PL (1) | PL1853700T3 (pt) |
| PT (1) | PT1853700E (pt) |
| SI (1) | SI1853700T1 (pt) |
| WO (1) | WO2006089613A1 (pt) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE602004025157D1 (de) * | 2003-09-23 | 2010-03-04 | Univ North Carolina | Zellen, die Vitamin-K-Reduktase und Vitamin-K-abhängiges Protein coexprimieren und deren Anwendung zur Verbesserung der Produktivität von diesem Vitamin-K-abhängigen Protein |
| ES2344413T3 (es) | 2003-10-14 | 2010-08-26 | Baxter International Inc. | Polipeptido vkorc1 de reciclaje de la vitamina k-epoxido, una diana terapeutica de la cumarina y sus derivados. |
| EP2295547B1 (en) | 2005-02-28 | 2017-09-20 | Baxalta GmbH | Recombinant co-expression of vitamin K epoxide reductase subunit 1 to improve vitamin K dependant protein expression |
| US20090325226A1 (en) | 2005-03-15 | 2009-12-31 | Stafford Darrel W | Methods and Compositions for Producing Active Vitamin K-Dependent Proteins |
| ATE486128T1 (de) * | 2005-12-02 | 2010-11-15 | Univ Wake Forest Health Sciences | Zusammensetzungen und verfahren zur erhöhung der produktion rekombinanter gamma-carboxylierter proteine |
| WO2007075976A2 (en) * | 2005-12-21 | 2007-07-05 | Inspiration Biopharmaceuticals, Inc. | Method of producing biologically active vitamin k dependent proteins by recombinant methods |
| UA101497C2 (ru) | 2008-04-24 | 2013-04-10 | Селтик Фарма Пег Лтд. | Конъюгаты фактора ix с увеличенным временем полужизни |
| WO2011003153A1 (en) * | 2009-07-10 | 2011-01-13 | Csl Limited | Method of increasing the expression yield of vitamin k-dependent proteins |
| EP2655607A4 (en) * | 2010-12-21 | 2014-05-14 | Univ North Carolina | METHOD AND COMPOSITIONS FOR PRODUCING ACTIVE VITAMIN K-DEPENDENT PROTEINS |
| JP2012139232A (ja) * | 2012-03-15 | 2012-07-26 | Univ Of North Carolina At Chapel Hill | 活性ビタミンｋ依存性タンパク質を生産するための方法及び組成物 |
| RU2535871C1 (ru) * | 2013-07-10 | 2014-12-20 | Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") | Плазмида для экспрессии в клетке китайского хомячка, клетка китайского хомячка - продуцент белка с Gla-доменом и способ получения белка с Gla-доменом |
| JP2014221048A (ja) * | 2014-06-16 | 2014-11-27 | ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル | 活性ビタミンｋ依存性タンパク質を生産するための方法及び組成物 |
| CN104293737B (zh) * | 2014-09-16 | 2017-06-13 | 太原博奥特生物技术有限公司 | 一种包含表达功能性重组人凝血因子ⅶ载体的宿主细胞及其高水平表达方法 |
| AU2016363789B2 (en) | 2015-12-02 | 2022-06-02 | CSL Behring Lengnau AG | Improved media for the expression of recombinant vitamin K-dependent proteins |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8822584D0 (en) | 1988-09-27 | 1988-11-02 | Abco Technology Ltd | Steam boiler system |
| WO1991001372A1 (en) | 1989-07-17 | 1991-02-07 | New England Medical Center Hospitals, Inc. | VITAMIN K-DEPENDENT η-CARBOXYLASE |
| JPH05508551A (ja) | 1990-07-23 | 1993-12-02 | ザイモジェネティクス，インコーポレイティド | ガンマーカルボキシラーゼおよび使用の方法 |
| US5268275A (en) | 1991-05-08 | 1993-12-07 | The University Of North Carolina At Chapel Hill | Vitamin K-dependent carboxylase |
| WO2002102994A2 (en) | 2001-03-21 | 2002-12-27 | Human Genome Sciences, Inc. | Human secreted proteins |
| WO2000003015A2 (en) | 1998-07-10 | 2000-01-20 | Incyte Pharmaceuticals, Inc. | Human transport protein homologs |
| KR100880624B1 (ko) | 2000-10-02 | 2009-01-30 | 노보 노르디스크 헬스 케어 악티엔게젤샤프트 | 비타민 ｋ-의존 단백질의 생산 방법 |
| DE602004025157D1 (de) | 2003-09-23 | 2010-03-04 | Univ North Carolina | Zellen, die Vitamin-K-Reduktase und Vitamin-K-abhängiges Protein coexprimieren und deren Anwendung zur Verbesserung der Produktivität von diesem Vitamin-K-abhängigen Protein |
| ES2344413T3 (es) * | 2003-10-14 | 2010-08-26 | Baxter International Inc. | Polipeptido vkorc1 de reciclaje de la vitamina k-epoxido, una diana terapeutica de la cumarina y sus derivados. |
| WO2006067116A1 (en) | 2004-12-21 | 2006-06-29 | Novo Nordisk Health Care Ag | Expression of gamma-carboxylated polypeptides in gamma-carboxylation deficient host systems |
| EP2295547B1 (en) | 2005-02-28 | 2017-09-20 | Baxalta GmbH | Recombinant co-expression of vitamin K epoxide reductase subunit 1 to improve vitamin K dependant protein expression |
| US20090325226A1 (en) | 2005-03-15 | 2009-12-31 | Stafford Darrel W | Methods and Compositions for Producing Active Vitamin K-Dependent Proteins |
-
2006
- 2006-01-27 EP EP10010933.9A patent/EP2295547B1/en not_active Revoked
- 2006-01-27 CN CN201310245632XA patent/CN103396998A/zh active Pending
- 2006-01-27 PL PL06706455T patent/PL1853700T3/pl unknown
- 2006-01-27 MX MX2012009048A patent/MX349285B/es unknown
- 2006-01-27 SI SI200631503T patent/SI1853700T1/sl unknown
- 2006-01-27 PT PT67064550T patent/PT1853700E/pt unknown
- 2006-01-27 DK DK06706455.0T patent/DK1853700T3/da active
- 2006-01-27 MX MX2007010489A patent/MX2007010489A/es active IP Right Grant
- 2006-01-27 WO PCT/EP2006/000734 patent/WO2006089613A1/en active Application Filing
- 2006-01-27 KR KR1020137002337A patent/KR20130025952A/ko not_active Application Discontinuation
- 2006-01-27 ES ES06706455T patent/ES2397530T3/es active Active
- 2006-01-27 US US11/342,299 patent/US9617523B2/en not_active Expired - Fee Related
- 2006-01-27 AU AU2006218216A patent/AU2006218216B2/en not_active Ceased
- 2006-01-27 EP EP06706455A patent/EP1853700B1/en not_active Revoked
- 2006-01-27 JP JP2007557348A patent/JP2008531026A/ja not_active Ceased
- 2006-01-27 CA CA002599284A patent/CA2599284A1/en not_active Abandoned
- 2006-01-27 KR KR1020077022018A patent/KR101310532B1/ko active IP Right Grant
- 2006-01-27 BR BRPI0607751A patent/BRPI0607751A8/pt not_active Application Discontinuation
- 2006-01-27 CN CNA2006800047124A patent/CN101124320A/zh active Pending
-
2007
- 2007-07-18 IL IL184696A patent/IL184696A/en not_active IP Right Cessation
-
2008
- 2008-04-01 HK HK08103591.9A patent/HK1113498A1/xx not_active IP Right Cessation
-
2011
- 2011-04-28 JP JP2011102480A patent/JP2011142923A/ja active Pending
-
2012
- 2012-12-21 JP JP2012279460A patent/JP5339391B2/ja not_active Expired - Fee Related
-
2013
- 2013-01-15 HR HRP20130028TT patent/HRP20130028T1/hr unknown
-
2017
- 2017-01-18 US US15/409,032 patent/US20170226487A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP2013063089A (ja) | 2013-04-11 |
| EP2295547A1 (en) | 2011-03-16 |
| KR20070110106A (ko) | 2007-11-15 |
| CN101124320A (zh) | 2008-02-13 |
| HK1113498A1 (en) | 2008-10-03 |
| HRP20130028T1 (hr) | 2013-02-28 |
| EP1853700A1 (en) | 2007-11-14 |
| EP2295547B1 (en) | 2017-09-20 |
| IL184696A (en) | 2013-09-30 |
| EP1853700B1 (en) | 2012-10-24 |
| KR101310532B1 (ko) | 2013-09-23 |
| MX349285B (es) | 2017-07-20 |
| US9617523B2 (en) | 2017-04-11 |
| WO2006089613A1 (en) | 2006-08-31 |
| PL1853700T3 (pl) | 2013-05-31 |
| MX2007010489A (es) | 2007-11-08 |
| BRPI0607751A2 (pt) | 2010-03-16 |
| JP2008531026A (ja) | 2008-08-14 |
| JP2011142923A (ja) | 2011-07-28 |
| AU2006218216A1 (en) | 2006-08-31 |
| CA2599284A1 (en) | 2006-08-31 |
| US20060194284A1 (en) | 2006-08-31 |
| SI1853700T1 (sl) | 2013-02-28 |
| US20170226487A1 (en) | 2017-08-10 |
| ES2397530T3 (es) | 2013-03-07 |
| AU2006218216B2 (en) | 2011-01-20 |
| DK1853700T3 (da) | 2013-01-07 |
| IL184696A0 (en) | 2007-12-03 |
| PT1853700E (pt) | 2013-01-14 |
| CN103396998A (zh) | 2013-11-20 |
| KR20130025952A (ko) | 2013-03-12 |
| JP5339391B2 (ja) | 2013-11-13 |
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