MX349285B - Co-expresion recombinante de reductasa de epoxido de vitamina k subunidad 1 para mejorar la expresion de proteina dependiente de vitamina k. - Google Patents

Co-expresion recombinante de reductasa de epoxido de vitamina k subunidad 1 para mejorar la expresion de proteina dependiente de vitamina k.

Info

Publication number
MX349285B
MX349285B MX2012009048A MX2012009048A MX349285B MX 349285 B MX349285 B MX 349285B MX 2012009048 A MX2012009048 A MX 2012009048A MX 2012009048 A MX2012009048 A MX 2012009048A MX 349285 B MX349285 B MX 349285B
Authority
MX
Mexico
Prior art keywords
vitamin
recombinant
expression
dependent protein
protein expression
Prior art date
Application number
MX2012009048A
Other languages
English (en)
Inventor
Scheiflinger Friedrich
Boehm Ernst
Original Assignee
Baxalta Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36130202&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX349285(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Baxalta Inc filed Critical Baxalta Inc
Publication of MX349285B publication Critical patent/MX349285B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0093—Oxidoreductases (1.) acting on CH or CH2 groups (1.17)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y117/00—Oxidoreductases acting on CH or CH2 groups (1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se refiere a un organismo huésped que contiene ácidos nucleicos recombinantes que codifica el complejo de reductasa de vitamina K subunidad 1 (KVORC1) y ácidos nucleicos recombinantes que codifican una proteína dependiente de vitamina K (VKD), en donde tanto el VKORC1 recombinante como la proteína VKD recombinante son expresados en tal organismo huésped. Además, la presente invención se refiere a un sistema de cultivo celular que comprende células que contienen tales ácidos nucleicos recombinantes y métodos para mejorar la productividad de expresión de proteína VKD recombinante en un organismo huésped que es cultivado en sistemas adecuados.
MX2012009048A 2005-02-28 2006-01-27 Co-expresion recombinante de reductasa de epoxido de vitamina k subunidad 1 para mejorar la expresion de proteina dependiente de vitamina k. MX349285B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65704105P 2005-02-28 2005-02-28
PCT/EP2006/000734 WO2006089613A1 (en) 2005-02-28 2006-01-27 Recombinant co-expression of vitamin k epoxide reductase subunit 1 to improve vitamin k dependent protein expression

Publications (1)

Publication Number Publication Date
MX349285B true MX349285B (es) 2017-07-20

Family

ID=36130202

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2012009048A MX349285B (es) 2005-02-28 2006-01-27 Co-expresion recombinante de reductasa de epoxido de vitamina k subunidad 1 para mejorar la expresion de proteina dependiente de vitamina k.
MX2007010489A MX2007010489A (es) 2005-02-28 2006-01-27 Co-expresion recombinante de reductasa de epoxido de vitamina k subunidad 1 para mejorar la expresion de proteina dependiente de vitamina k.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2007010489A MX2007010489A (es) 2005-02-28 2006-01-27 Co-expresion recombinante de reductasa de epoxido de vitamina k subunidad 1 para mejorar la expresion de proteina dependiente de vitamina k.

Country Status (18)

Country Link
US (2) US9617523B2 (es)
EP (2) EP2295547B1 (es)
JP (3) JP2008531026A (es)
KR (2) KR20130025952A (es)
CN (2) CN103396998A (es)
AU (1) AU2006218216B2 (es)
BR (1) BRPI0607751A8 (es)
CA (1) CA2599284A1 (es)
DK (1) DK1853700T3 (es)
ES (1) ES2397530T3 (es)
HK (1) HK1113498A1 (es)
HR (1) HRP20130028T1 (es)
IL (1) IL184696A (es)
MX (2) MX349285B (es)
PL (1) PL1853700T3 (es)
PT (1) PT1853700E (es)
SI (1) SI1853700T1 (es)
WO (1) WO2006089613A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL1842920T5 (pl) 2003-09-23 2018-04-30 University Of North Carolina At Chapel Hill Komórki koekspresjonujące reduktazę witaminy K i białko zależne od witaminy K oraz ich zastosowanie do poprawienia wydajności wytwarzania tego białka zależnego od witaminy K
PT1673450E (pt) 2003-10-14 2010-05-11 Baxter Int Polipéptido de reciclagem de epóxido de vitamina k, vkorc1, um alvo terapêutico de cumarina e seus derivados
EP2295547B1 (en) 2005-02-28 2017-09-20 Baxalta GmbH Recombinant co-expression of vitamin K epoxide reductase subunit 1 to improve vitamin K dependant protein expression
AU2005329450A1 (en) * 2005-03-15 2006-09-28 University Of North Carolina At Chapel Hill Methods and compositions for producing active Vitamin K-dependent proteins
US8647868B2 (en) * 2005-12-02 2014-02-11 Wake Forest University Health Sciences Compositions and methods for increasing production of recombinant gamma-carboxylated proteins
PL1969127T5 (pl) * 2005-12-21 2018-02-28 Cnj Holdings, Inc Metoda wytwarzania biologicznie czynnych białek zależnych od witaminy K metodami rekombinacji
KR20110016440A (ko) 2008-04-24 2011-02-17 셀틱 파르마 피이지 엘티디. 연장된 반감기를 가진 제 ⅰⅹ 인자 접합체
JP5781067B2 (ja) * 2009-07-10 2015-09-16 シーエスエル、リミテッド ビタミンｋ依存性タンパク質の発現収率を増加させる方法
EP2655607A4 (en) * 2010-12-21 2014-05-14 Univ North Carolina METHOD AND COMPOSITIONS FOR PRODUCING ACTIVE VITAMIN K-DEPENDENT PROTEINS
JP2012139232A (ja) * 2012-03-15 2012-07-26 Univ Of North Carolina At Chapel Hill 活性ビタミンｋ依存性タンパク質を生産するための方法及び組成物
RU2535871C1 (ru) * 2013-07-10 2014-12-20 Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") Плазмида для экспрессии в клетке китайского хомячка, клетка китайского хомячка - продуцент белка с Gla-доменом и способ получения белка с Gla-доменом
JP2014221048A (ja) * 2014-06-16 2014-11-27 ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル 活性ビタミンｋ依存性タンパク質を生産するための方法及び組成物
CN104293737B (zh) * 2014-09-16 2017-06-13 太原博奥特生物技术有限公司 一种包含表达功能性重组人凝血因子ⅶ载体的宿主细胞及其高水平表达方法
US20180362952A1 (en) 2015-12-02 2018-12-20 Csl Behring Recombinant Facility Ag Improved media for the expression of recombinant vitamin k-dependent proteins

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8822584D0 (en) 1988-09-27 1988-11-02 Abco Technology Ltd Steam boiler system
WO1991001372A1 (en) 1989-07-17 1991-02-07 New England Medical Center Hospitals, Inc. VITAMIN K-DEPENDENT η-CARBOXYLASE
EP0540650A1 (en) 1990-07-23 1993-05-12 Zymogenetics, Inc. Gamma-carboxylase and methods of use
US5268275A (en) 1991-05-08 1993-12-07 The University Of North Carolina At Chapel Hill Vitamin K-dependent carboxylase
CA2332307A1 (en) 1998-07-10 2000-01-20 Incyte Pharmaceuticals, Inc. Human transport protein homologs
WO2002029083A2 (en) 2000-10-02 2002-04-11 Novo Nordisk A/S Industrial-scale serum-free production of recombinant proteins in mammalian cells
EP1390390A4 (en) 2001-03-21 2009-07-08 Human Genome Sciences Inc HUMAN SECRETED PROTEINS
PL1842920T5 (pl) 2003-09-23 2018-04-30 University Of North Carolina At Chapel Hill Komórki koekspresjonujące reduktazę witaminy K i białko zależne od witaminy K oraz ich zastosowanie do poprawienia wydajności wytwarzania tego białka zależnego od witaminy K
PT1673450E (pt) 2003-10-14 2010-05-11 Baxter Int Polipéptido de reciclagem de epóxido de vitamina k, vkorc1, um alvo terapêutico de cumarina e seus derivados
EP1831363A1 (en) * 2004-12-21 2007-09-12 Novo Nordisk Health Care AG Expression of gamma-carboxylated polypeptides in gamma-carboxylation deficient host systems
EP2295547B1 (en) 2005-02-28 2017-09-20 Baxalta GmbH Recombinant co-expression of vitamin K epoxide reductase subunit 1 to improve vitamin K dependant protein expression
AU2005329450A1 (en) 2005-03-15 2006-09-28 University Of North Carolina At Chapel Hill Methods and compositions for producing active Vitamin K-dependent proteins

Also Published As

Publication number Publication date
EP2295547B1 (en) 2017-09-20
HK1113498A1 (en) 2008-10-03
PT1853700E (pt) 2013-01-14
EP1853700A1 (en) 2007-11-14
US9617523B2 (en) 2017-04-11
JP2008531026A (ja) 2008-08-14
HRP20130028T1 (hr) 2013-02-28
AU2006218216B2 (en) 2011-01-20
EP1853700B1 (en) 2012-10-24
ES2397530T3 (es) 2013-03-07
IL184696A (en) 2013-09-30
CA2599284A1 (en) 2006-08-31
MX2007010489A (es) 2007-11-08
CN101124320A (zh) 2008-02-13
KR20070110106A (ko) 2007-11-15
BRPI0607751A2 (pt) 2010-03-16
US20060194284A1 (en) 2006-08-31
SI1853700T1 (sl) 2013-02-28
AU2006218216A1 (en) 2006-08-31
EP2295547A1 (en) 2011-03-16
PL1853700T3 (pl) 2013-05-31
KR101310532B1 (ko) 2013-09-23
WO2006089613A1 (en) 2006-08-31
JP2011142923A (ja) 2011-07-28
JP2013063089A (ja) 2013-04-11
BRPI0607751A8 (pt) 2015-10-27
CN103396998A (zh) 2013-11-20
JP5339391B2 (ja) 2013-11-13
IL184696A0 (en) 2007-12-03
US20170226487A1 (en) 2017-08-10
DK1853700T3 (da) 2013-01-07
KR20130025952A (ko) 2013-03-12

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