MX349285B - Co-expresion recombinante de reductasa de epoxido de vitamina k subunidad 1 para mejorar la expresion de proteina dependiente de vitamina k. - Google Patents

Co-expresion recombinante de reductasa de epoxido de vitamina k subunidad 1 para mejorar la expresion de proteina dependiente de vitamina k.

Info

Publication number
MX349285B
MX349285B MX2012009048A MX2012009048A MX349285B MX 349285 B MX349285 B MX 349285B MX 2012009048 A MX2012009048 A MX 2012009048A MX 2012009048 A MX2012009048 A MX 2012009048A MX 349285 B MX349285 B MX 349285B
Authority
MX
Mexico
Prior art keywords
vitamin
recombinant
expression
dependent protein
protein expression
Prior art date
Application number
MX2012009048A
Other languages
English (en)
Inventor
Scheiflinger Friedrich
Boehm Ernst
Original Assignee
Baxalta Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36130202&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX349285(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Baxalta Inc filed Critical Baxalta Inc
Publication of MX349285B publication Critical patent/MX349285B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0093—Oxidoreductases (1.) acting on CH or CH2 groups (1.17)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y117/00—Oxidoreductases acting on CH or CH2 groups (1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Abstract

La presente invención se refiere a un organismo huésped que contiene ácidos nucleicos recombinantes que codifica el complejo de reductasa de vitamina K subunidad 1 (KVORC1) y ácidos nucleicos recombinantes que codifican una proteína dependiente de vitamina K (VKD), en donde tanto el VKORC1 recombinante como la proteína VKD recombinante son expresados en tal organismo huésped. Además, la presente invención se refiere a un sistema de cultivo celular que comprende células que contienen tales ácidos nucleicos recombinantes y métodos para mejorar la productividad de expresión de proteína VKD recombinante en un organismo huésped que es cultivado en sistemas adecuados.
MX2012009048A 2005-02-28 2006-01-27 Co-expresion recombinante de reductasa de epoxido de vitamina k subunidad 1 para mejorar la expresion de proteina dependiente de vitamina k. MX349285B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65704105P 2005-02-28 2005-02-28
PCT/EP2006/000734 WO2006089613A1 (en) 2005-02-28 2006-01-27 Recombinant co-expression of vitamin k epoxide reductase subunit 1 to improve vitamin k dependent protein expression

Publications (1)

Publication Number Publication Date
MX349285B true MX349285B (es) 2017-07-20

Family

ID=36130202

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2012009048A MX349285B (es) 2005-02-28 2006-01-27 Co-expresion recombinante de reductasa de epoxido de vitamina k subunidad 1 para mejorar la expresion de proteina dependiente de vitamina k.
MX2007010489A MX2007010489A (es) 2005-02-28 2006-01-27 Co-expresion recombinante de reductasa de epoxido de vitamina k subunidad 1 para mejorar la expresion de proteina dependiente de vitamina k.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2007010489A MX2007010489A (es) 2005-02-28 2006-01-27 Co-expresion recombinante de reductasa de epoxido de vitamina k subunidad 1 para mejorar la expresion de proteina dependiente de vitamina k.

Country Status (18)

Country Link
US (2) US9617523B2 (es)
EP (2) EP2295547B1 (es)
JP (3) JP2008531026A (es)
KR (2) KR101310532B1 (es)
CN (2) CN101124320A (es)
AU (1) AU2006218216B2 (es)
BR (1) BRPI0607751A8 (es)
CA (1) CA2599284A1 (es)
DK (1) DK1853700T3 (es)
ES (1) ES2397530T3 (es)
HK (1) HK1113498A1 (es)
HR (1) HRP20130028T1 (es)
IL (1) IL184696A (es)
MX (2) MX349285B (es)
PL (1) PL1853700T3 (es)
PT (1) PT1853700E (es)
SI (1) SI1853700T1 (es)
WO (1) WO2006089613A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2339710T5 (es) * 2003-09-23 2017-10-05 University Of North Carolina At Chapel Hill Células que coexpresan vitamina K reductasa y proteína dependiente de vitamina K y uso de las mismas para mejorar la productividad de dicha proteína dependiente de vitamina K
EP1673450B1 (en) 2003-10-14 2010-04-28 Baxter International Inc. Vitamin k epoxide recycling polypeptide vkorc1, a therapeutic target of coumarin and their derivatives
WO2006089613A1 (en) 2005-02-28 2006-08-31 Baxter International Inc. Recombinant co-expression of vitamin k epoxide reductase subunit 1 to improve vitamin k dependent protein expression
US20090325226A1 (en) 2005-03-15 2009-12-31 Stafford Darrel W Methods and Compositions for Producing Active Vitamin K-Dependent Proteins
US8647868B2 (en) * 2005-12-02 2014-02-11 Wake Forest University Health Sciences Compositions and methods for increasing production of recombinant gamma-carboxylated proteins
US20080045453A1 (en) * 2005-12-21 2008-02-21 Drohan William N Method of producing biologically active vitamin K dependent proteins by recombinant methods
MX2010011672A (es) 2008-04-24 2011-03-02 Celtic Pharma Peg Ltd Conjugados del factor ix con vidas medias extendidas.
CN102471794B (zh) * 2009-07-10 2014-10-29 Csl有限公司 提高维生素k依赖性蛋白质的表达产量的方法
US9631002B2 (en) 2010-12-21 2017-04-25 The University Of North Carolina At Chapel Hill Methods and compositions for producing active vitamin K-dependent proteins
JP2012139232A (ja) * 2012-03-15 2012-07-26 Univ Of North Carolina At Chapel Hill 活性ビタミンｋ依存性タンパク質を生産するための方法及び組成物
RU2535871C1 (ru) * 2013-07-10 2014-12-20 Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") Плазмида для экспрессии в клетке китайского хомячка, клетка китайского хомячка - продуцент белка с Gla-доменом и способ получения белка с Gla-доменом
JP2014221048A (ja) * 2014-06-16 2014-11-27 ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル 活性ビタミンｋ依存性タンパク質を生産するための方法及び組成物
CN104293737B (zh) * 2014-09-16 2017-06-13 太原博奥特生物技术有限公司 一种包含表达功能性重组人凝血因子ⅶ载体的宿主细胞及其高水平表达方法
EP3383894B1 (en) 2015-12-02 2020-05-06 CSL Behring Lengnau AG Improved media for the expression of recombinant vitamin k-dependent proteins

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8822584D0 (en) 1988-09-27 1988-11-02 Abco Technology Ltd Steam boiler system
WO1991001372A1 (en) 1989-07-17 1991-02-07 New England Medical Center Hospitals, Inc. VITAMIN K-DEPENDENT η-CARBOXYLASE
CA2087970A1 (en) 1990-07-23 1992-01-24 Kathleen L. Berkner Gamma-carboxylase and methods of use
US5268275A (en) 1991-05-08 1993-12-07 The University Of North Carolina At Chapel Hill Vitamin K-dependent carboxylase
WO2003004622A2 (en) 2001-03-21 2003-01-16 Human Genome Sciences, Inc. Human secreted proteins
CA2332307A1 (en) 1998-07-10 2000-01-20 Incyte Pharmaceuticals, Inc. Human transport protein homologs
US6903069B2 (en) 2000-10-02 2005-06-07 Novo Nordisk Health Care A/S Factor VII glycoforms
ES2339710T5 (es) 2003-09-23 2017-10-05 University Of North Carolina At Chapel Hill Células que coexpresan vitamina K reductasa y proteína dependiente de vitamina K y uso de las mismas para mejorar la productividad de dicha proteína dependiente de vitamina K
EP1673450B1 (en) 2003-10-14 2010-04-28 Baxter International Inc. Vitamin k epoxide recycling polypeptide vkorc1, a therapeutic target of coumarin and their derivatives
WO2006067116A1 (en) * 2004-12-21 2006-06-29 Novo Nordisk Health Care Ag Expression of gamma-carboxylated polypeptides in gamma-carboxylation deficient host systems
WO2006089613A1 (en) 2005-02-28 2006-08-31 Baxter International Inc. Recombinant co-expression of vitamin k epoxide reductase subunit 1 to improve vitamin k dependent protein expression
US20090325226A1 (en) 2005-03-15 2009-12-31 Stafford Darrel W Methods and Compositions for Producing Active Vitamin K-Dependent Proteins

Also Published As

Publication number Publication date
KR101310532B1 (ko) 2013-09-23
CA2599284A1 (en) 2006-08-31
PT1853700E (pt) 2013-01-14
US20170226487A1 (en) 2017-08-10
EP2295547A1 (en) 2011-03-16
HK1113498A1 (en) 2008-10-03
JP2008531026A (ja) 2008-08-14
CN101124320A (zh) 2008-02-13
EP2295547B1 (en) 2017-09-20
PL1853700T3 (pl) 2013-05-31
AU2006218216B2 (en) 2011-01-20
SI1853700T1 (sl) 2013-02-28
EP1853700A1 (en) 2007-11-14
US20060194284A1 (en) 2006-08-31
WO2006089613A1 (en) 2006-08-31
JP5339391B2 (ja) 2013-11-13
US9617523B2 (en) 2017-04-11
JP2011142923A (ja) 2011-07-28
AU2006218216A1 (en) 2006-08-31
BRPI0607751A2 (pt) 2010-03-16
KR20070110106A (ko) 2007-11-15
MX2007010489A (es) 2007-11-08
DK1853700T3 (da) 2013-01-07
EP1853700B1 (en) 2012-10-24
CN103396998A (zh) 2013-11-20
IL184696A0 (en) 2007-12-03
ES2397530T3 (es) 2013-03-07
JP2013063089A (ja) 2013-04-11
HRP20130028T1 (hr) 2013-02-28
KR20130025952A (ko) 2013-03-12
IL184696A (en) 2013-09-30
BRPI0607751A8 (pt) 2015-10-27

Similar Documents

Publication Publication Date Title
MX2007010489A (es) Co-expresion recombinante de reductasa de epoxido de vitamina k subunidad 1 para mejorar la expresion de proteina dependiente de vitamina k.
MX2007003906A (es) Metodos y composiciones para mejorar la produccion de proteinas recombinantes.
BRPI0814781A8 (pt) ''proteína, sequência de ácido nucleico, método para a produção de ácido itacônico, vetor, célula hospedeira, e, uso de um ácido cis-aconítico descarboxilase''
UA92157C2 (ru) Способ продуцирования гормона роста
TW200716753A (en) Vector
MX2009002388A (es) Uso de celulas humanas originarias de leucemia mieloide para la expresion de anticuerpos.
WO2006070370A3 (en) Stem cells culture systems
MY185872A (en) Cell culture improvements
MY169472A (en) Refolding of recombinant proteins
WO2007002086A3 (en) Suspension culture of human embryonic stem cells
MX2021015450A (es) Reactivos y métodos para la replicación, transcripción y traducción en organismos semisintéticos.
BRPI0618449A2 (pt) métodos para produção de isoformas de receptor e ligante
HK1086297A1 (en) Circular dna molecule having a conditional origin of replication and process for preparation
WO2008078646A1 (ja) アミノ酸又は核酸の結晶の分離方法
WO2008020333A3 (en) Nucleic acid encoding n-methylputrescine oxidase and uses thereof
EP1841871A4 (en) NEW L-LYSINE INDUCIBLE PROMOTER
MX2013005853A (es) Enzimas de fusion que tienen actividad de n-acetilglucosaminil transferasa.
WO2006004728A3 (en) Cell culture medium comprising transition metals or trace elements
EP1983048A4 (en) NEW PROTEIN EXPRESSION SYSTEM
BRPI0410562B8 (pt) proteína de veículo e seu método de produção em uma planta, ácido nucleico isolado, vetor de expressão e célula hospedeira
ATE336567T1 (de) Zellkulturmedium
WO2003057897A3 (en) Recombinant protein expression
TW200716744A (en) Genetically modified microorganism and process for production of macrolide compound using the microorganism
MX2010010309A (es) Ceculas hospederas y metodos para producir proteinas que contienen enlace de disulfuro.
WO2008108325A1 (ja) 新規なβ－ガラクトシド－α２，６－シアル酸転移酵素、それをコードする遺伝子および酵素活性を向上させる方法