BRPI0500474A - adsorção de ácidos nucléicos para uma fase sólida - Google Patents

adsorção de ácidos nucléicos para uma fase sólida

Info

Publication number
BRPI0500474A
BRPI0500474A BRPI0500474-8A BRPI0500474A BRPI0500474A BR PI0500474 A BRPI0500474 A BR PI0500474A BR PI0500474 A BRPI0500474 A BR PI0500474A BR PI0500474 A BRPI0500474 A BR PI0500474A
Authority
BR
Brazil
Prior art keywords
nucleic acids
solid phase
mixture
adsorption
biological sample
Prior art date
Application number
BRPI0500474-8A
Other languages
English (en)
Inventor
Ralf Zielenski
Klaus Geissler
Thomas Walter
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BRPI0500474A publication Critical patent/BRPI0500474A/pt
Publication of BRPI0500474B1 publication Critical patent/BRPI0500474B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16D—COUPLINGS FOR TRANSMITTING ROTATION; CLUTCHES; BRAKES
    • F16D55/00—Brakes with substantially-radial braking surfaces pressed together in axial direction, e.g. disc brakes
    • F16D55/02—Brakes with substantially-radial braking surfaces pressed together in axial direction, e.g. disc brakes with axially-movable discs or pads pressed against axially-located rotating members
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16D—COUPLINGS FOR TRANSMITTING ROTATION; CLUTCHES; BRAKES
    • F16D63/00—Brakes not otherwise provided for; Brakes combining more than one of the types of groups F16D49/00 - F16D61/00
    • F16D63/006—Positive locking brakes
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16D—COUPLINGS FOR TRANSMITTING ROTATION; CLUTCHES; BRAKES
    • F16D65/00—Parts or details
    • F16D65/14—Actuating mechanisms for brakes; Means for initiating operation at a predetermined position
    • F16D65/16—Actuating mechanisms for brakes; Means for initiating operation at a predetermined position arranged in or on the brake
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16D—COUPLINGS FOR TRANSMITTING ROTATION; CLUTCHES; BRAKES
    • F16D2125/00—Components of actuators
    • F16D2125/18—Mechanical mechanisms
    • F16D2125/20—Mechanical mechanisms converting rotation to linear movement or vice versa
    • F16D2125/22—Mechanical mechanisms converting rotation to linear movement or vice versa acting transversely to the axis of rotation
    • F16D2125/28—Cams; Levers with cams

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Mechanical Engineering (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

"ADSORçãO DE áCIDOS NUCLéICOS PARA UMA FASE SóLIDA". A presente invenção refere-se a um método para adsorção, isto é, ligação não-covalentemente de ácidos nucléicos a uma fase sólida usando um procedimento de duas etapas. Ainda, a presente invenção refere-se a um método para isolamento de ácidos nucléicos a partir de uma amostra biológica. Na primeira etapa do procedimento, a lise é realizada misturando a amostra biológica com um tampão de lise aquoso contendo um agente caotrópico e incubação da mistura; na segunda etapa, a concentração do agente caotrópico na mistura é aumentada e a mistura é contatada com a fase sólida, com o que os ácidos nucléicos na fase líquida são adsorvidos na fase sólida.
BRPI0500474-8A 2004-02-20 2005-02-17 Métodos para isolar e para adsorção de um ácido nucléico a partir de uma amostra biológica BRPI0500474B1 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP04003898 2004-02-20

Publications (2)

Publication Number Publication Date
BRPI0500474A true BRPI0500474A (pt) 2006-04-04
BRPI0500474B1 BRPI0500474B1 (pt) 2015-01-27

Family

ID=34878164

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0500474-8A BRPI0500474B1 (pt) 2004-02-20 2005-02-17 Métodos para isolar e para adsorção de um ácido nucléico a partir de uma amostra biológica

Country Status (16)

Country Link
US (1) US7491495B2 (pt)
EP (1) EP1566437B1 (pt)
JP (1) JP4271663B2 (pt)
KR (1) KR100692941B1 (pt)
CN (1) CN1310938C (pt)
AT (1) ATE364694T1 (pt)
AU (1) AU2005200670B2 (pt)
BR (1) BRPI0500474B1 (pt)
CA (1) CA2497493C (pt)
DE (1) DE602005001331T2 (pt)
DK (1) DK1566437T3 (pt)
ES (1) ES2289611T3 (pt)
HK (1) HK1084122A1 (pt)
MX (1) MXPA05001815A (pt)
PL (1) PL1566437T3 (pt)
SG (1) SG114738A1 (pt)

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Also Published As

Publication number Publication date
KR100692941B1 (ko) 2007-03-12
JP2005230012A (ja) 2005-09-02
US7491495B2 (en) 2009-02-17
ES2289611T3 (es) 2008-02-01
ATE364694T1 (de) 2007-07-15
EP1566437B1 (en) 2007-06-13
JP4271663B2 (ja) 2009-06-03
BRPI0500474B1 (pt) 2015-01-27
CN1680421A (zh) 2005-10-12
KR20060042961A (ko) 2006-05-15
SG114738A1 (en) 2005-09-28
CN1310938C (zh) 2007-04-18
HK1084122A1 (en) 2006-07-21
DK1566437T3 (da) 2007-10-15
AU2005200670A1 (en) 2005-09-08
DE602005001331D1 (de) 2007-07-26
CA2497493C (en) 2009-06-16
DE602005001331T2 (de) 2008-02-21
US20050214926A1 (en) 2005-09-29
MXPA05001815A (es) 2005-08-24
PL1566437T3 (pl) 2007-11-30
AU2005200670B2 (en) 2007-05-03
CA2497493A1 (en) 2005-08-20
EP1566437A1 (en) 2005-08-24

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