BRPI0113048B8 - cepas saccharomyces cerevisiae com genes ypr1 e gcy1 modificados e tendo atividade 20 alfa-hidroxiesteróide dehidrogenase (20 alfa-hsd) reduzida, seus usos na preparação de compostos esteroidais, bem como processos de modificação de hidroxiprogesterona e 11-deoxicortisol - Google Patents

cepas saccharomyces cerevisiae com genes ypr1 e gcy1 modificados e tendo atividade 20 alfa-hidroxiesteróide dehidrogenase (20 alfa-hsd) reduzida, seus usos na preparação de compostos esteroidais, bem como processos de modificação de hidroxiprogesterona e 11-deoxicortisol

Info

Publication number
BRPI0113048B8
BRPI0113048B8 BRPI0113048A BR0113048A BRPI0113048B8 BR PI0113048 B8 BRPI0113048 B8 BR PI0113048B8 BR PI0113048 A BRPI0113048 A BR PI0113048A BR 0113048 A BR0113048 A BR 0113048A BR PI0113048 B8 BRPI0113048 B8 BR PI0113048B8
Authority
BR
Brazil
Prior art keywords
alpha
processes
well
activity
gcy1
Prior art date
Application number
BRPI0113048A
Other languages
English (en)
Other versions
BRPI0113048B1 (pt
BR0113048A (pt
Inventor
Dumas Bruno
Degryse Eric
Cauet Gilles
Achstetter Tilman
Original Assignee
Aventis Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Sa filed Critical Aventis Pharma Sa
Publication of BR0113048A publication Critical patent/BR0113048A/pt
Publication of BRPI0113048B1 publication Critical patent/BRPI0113048B1/pt
Publication of BRPI0113048B8 publication Critical patent/BRPI0113048B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C12N9/0087—Steroid 21-monooxygenase (1.14.99.10)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Endocrinology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Diabetes (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Steroid Compounds (AREA)

Abstract

"lêvedos modificados e utilizações, principalmente para a produção de derivados esteroidianos". a presente invenção refere-se a novas cepas de lêvedo, processos e construções genéticas para seu preparo, assim como sua utilização para a síntese ou a modificação de compostos esteroidianos. mais particularmente, a invenção descreve cepas que apresentam uma atividade de tipo 20<244>-hsd diminuída, notadamente por modificação dos genes gcy1 e/ou ypr1. as cepas de lêvedo da invenção permitem melhorar a eficácia da síntese ou aumentar a seletividade ou os rendimentos do processo, assim como a qualidade do produto final. as cepas, processos e compostos da invenção são úteis na pesquisa, no desenvolvimento e na produção de produtos com atividade terapêutica ou profilática, no homem ou no animal, notadamente de derivados esteroidianos.
BRPI0113048A 2000-08-08 2001-07-24 cepas saccharomyces cerevisiae com genes ypr1 e gcy1 modificados e tendo atividade 20 alfa-hidroxiesteróide dehidrogenase (20 alfa-hsd) reduzida, seus usos na preparação de compostos esteroidais, bem como processos de modificação de hidroxiprogesterona e 11-deoxicortisol BRPI0113048B8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0010437A FR2812884B1 (fr) 2000-08-08 2000-08-08 Levures modifiees et utilisations, notamment pour la production de derives steroidiens
PCT/FR2001/002417 WO2002012536A1 (fr) 2000-08-08 2001-07-24 Levures modifiees et utilisations, notamment pour la production de derives steroidiens

Publications (3)

Publication Number Publication Date
BR0113048A BR0113048A (pt) 2003-07-01
BRPI0113048B1 BRPI0113048B1 (pt) 2015-12-22
BRPI0113048B8 true BRPI0113048B8 (pt) 2021-05-25

Family

ID=8853403

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0113048A BRPI0113048B8 (pt) 2000-08-08 2001-07-24 cepas saccharomyces cerevisiae com genes ypr1 e gcy1 modificados e tendo atividade 20 alfa-hidroxiesteróide dehidrogenase (20 alfa-hsd) reduzida, seus usos na preparação de compostos esteroidais, bem como processos de modificação de hidroxiprogesterona e 11-deoxicortisol

Country Status (22)

Country Link
US (2) US7879592B2 (pt)
EP (1) EP1313873B1 (pt)
JP (1) JP4864277B2 (pt)
KR (1) KR100860702B1 (pt)
AR (1) AR030080A1 (pt)
AT (1) ATE390479T1 (pt)
AU (2) AU7856201A (pt)
BR (1) BRPI0113048B8 (pt)
CA (1) CA2418370C (pt)
CY (1) CY1108080T1 (pt)
DE (1) DE60133396T2 (pt)
DK (1) DK1313873T3 (pt)
ES (1) ES2300347T3 (pt)
FR (1) FR2812884B1 (pt)
IL (2) IL154294A0 (pt)
MX (1) MXPA03000702A (pt)
NO (1) NO334484B1 (pt)
NZ (1) NZ523612A (pt)
PT (1) PT1313873E (pt)
TW (1) TWI315739B (pt)
WO (1) WO2002012536A1 (pt)
ZA (1) ZA200300376B (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2812884B1 (fr) * 2000-08-08 2002-11-08 Aventis Pharma Sa Levures modifiees et utilisations, notamment pour la production de derives steroidiens
FR2820145B1 (fr) * 2001-01-31 2004-01-23 Aventis Pharma Sa Souche de levure produisant des steroides de facon autonome
FR2976949B1 (fr) * 2011-06-21 2015-08-28 Sanofi Sa Procede de preparation de levures genetiquement transformees capables de produire une molecule d'interet a haut titre

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5571250A (en) 1978-11-22 1980-05-29 Hatano Kk Paper for paper box with lid and method of assembling paper box with lid by using same
CA1159754A (en) 1980-06-27 1984-01-03 Roxane N. Dikeman Process for preparing limulus lysate
JPS62181571A (ja) 1986-08-11 1987-08-08 Canon Inc 画像処理装置
JPS63181571A (ja) 1987-01-22 1988-07-26 Matsushita Electric Ind Co Ltd 自動焦点整合装置
JPS6447380A (en) 1987-08-19 1989-02-21 Agency Ind Science Techn Steroid-oxidizing yeast strain
JPS6471250A (en) 1987-09-11 1989-03-16 Nec Corp Multi-function telephone set
EP0340878B1 (en) 1988-05-06 2001-05-16 Aventis Pharma S.A. Process for the biochemical oxidation of steroids and genetically engineered cells to be used therefor
JPH0231680A (ja) * 1988-07-22 1990-02-01 Agency Of Ind Science & Technol チトクロムＰ４５０ｃ↓２↓１産生酵母菌株
CN1042567A (zh) 1988-09-23 1990-05-30 吉斯特-布罗卡迪斯公司 类固醇多步氧化方法和所用的遗传工程细胞
JPH02249488A (ja) * 1989-03-22 1990-10-05 Sumitomo Chem Co Ltd チトクロムＰ４５０↓ｃ↓２↓１と酵母ＮＡＤＰＨ‐チトクロムＰ４５０還元酵素の融合酸化酵素、該酵素をコードする遺伝子および該酵素の製造方法
JP2645130B2 (ja) 1989-03-31 1997-08-25 日清製粉株式会社 ステロイド誘導体
FR2678611B1 (fr) 1991-07-04 1995-01-20 Roussel Uclaf Nouveaux esters pyrethrinouides de l'alcool 1,3,4,5,6,7-hexahydro 1,3-dioxo-2h-isoindol-2-yl-methylique, leur procede de preparation et leur application comme pesticides.
JP3363197B2 (ja) 1992-06-18 2003-01-08 麒麟麦酒株式会社 アルコールアセチルトランスフェラーゼ遺伝子およびその利用
US5547868A (en) 1993-06-09 1996-08-20 Regents Of The University Of California Cholesterol disposal fusion enzymes
IL116872A (en) 1995-02-15 2004-12-15 Aventis Pharma Sa Delta 5,7-Strol Reductase from Arbidopsis Thaliana
JP4093598B2 (ja) 1995-03-14 2008-06-04 大関株式会社 新規なアルコールアセチルトランスフェラーゼ遺伝子
DE19744212B4 (de) 1997-09-30 2006-01-19 Schering Ag Verfahren zur Herstellung von Ergosterol und dessen Zwischenprodukten mittels rekombinanter Hefen
US6303302B1 (en) * 1997-11-19 2001-10-16 The Whitehead Institute For Biomedical Research Regulation of fungal gene expression
FR2774393B1 (fr) 1998-02-05 2000-03-24 Hoechst Marion Roussel Inc Souches de levure ayant le gene atf2 interrompu et leurs applications
FR2799206A1 (fr) 1999-10-05 2001-04-06 Transgene Sa Procede de production de steroides modifies par fermentation de levures
FR2812884B1 (fr) 2000-08-08 2002-11-08 Aventis Pharma Sa Levures modifiees et utilisations, notamment pour la production de derives steroidiens
FR2820145B1 (fr) 2001-01-31 2004-01-23 Aventis Pharma Sa Souche de levure produisant des steroides de facon autonome

Also Published As

Publication number Publication date
US8173402B2 (en) 2012-05-08
KR20030024842A (ko) 2003-03-26
PT1313873E (pt) 2008-05-27
ATE390479T1 (de) 2008-04-15
KR100860702B1 (ko) 2008-09-26
US20110104749A1 (en) 2011-05-05
US7879592B2 (en) 2011-02-01
JP2004505634A (ja) 2004-02-26
CY1108080T1 (el) 2014-02-12
WO2002012536A1 (fr) 2002-02-14
DK1313873T3 (da) 2008-07-14
DE60133396T2 (de) 2009-07-09
BRPI0113048B1 (pt) 2015-12-22
AR030080A1 (es) 2003-08-13
EP1313873A1 (fr) 2003-05-28
AU2001278562B2 (en) 2006-07-20
ES2300347T3 (es) 2008-06-16
NO334484B1 (no) 2014-03-17
MXPA03000702A (es) 2003-06-04
TWI315739B (en) 2009-10-11
NO20030606D0 (no) 2003-02-07
JP4864277B2 (ja) 2012-02-01
FR2812884B1 (fr) 2002-11-08
FR2812884A1 (fr) 2002-02-15
NO20030606L (no) 2003-02-07
CA2418370C (fr) 2014-03-11
IL154294A0 (en) 2003-09-17
DE60133396D1 (de) 2008-05-08
NZ523612A (en) 2004-11-26
BR0113048A (pt) 2003-07-01
US20040082025A1 (en) 2004-04-29
ZA200300376B (en) 2004-01-22
EP1313873B1 (fr) 2008-03-26
AU7856201A (en) 2002-02-18
IL154294A (en) 2010-05-31
CA2418370A1 (fr) 2002-02-14

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