BRPI0607623A2 - cassete de expressão de gene e um transformante, e um método para produção de 2-deóxi-scyllo-inosose e um método para purificar 2-deóxi-scyllo-inosose usando referido transformante - Google Patents

cassete de expressão de gene e um transformante, e um método para produção de 2-deóxi-scyllo-inosose e um método para purificar 2-deóxi-scyllo-inosose usando referido transformante

Info

Publication number
BRPI0607623A2
BRPI0607623A2 BRPI0607623-8A BRPI0607623A BRPI0607623A2 BR PI0607623 A2 BRPI0607623 A2 BR PI0607623A2 BR PI0607623 A BRPI0607623 A BR PI0607623A BR PI0607623 A2 BRPI0607623 A2 BR PI0607623A2
Authority
BR
Brazil
Prior art keywords
inosose
scyllo
deoxy
transformant
expression cassette
Prior art date
Application number
BRPI0607623-8A
Other languages
English (en)
Inventor
Masamichi Takagi
Takahisa Kogure
Naoki Wakisaka
Hiroaki Takaku
Katsumi Ajisaka
Tatsuo Miyazaki
Masao Hirayama
Original Assignee
Niigata Bio Res Park Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37086767&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0607623(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Niigata Bio Res Park Inc filed Critical Niigata Bio Res Park Inc
Publication of BRPI0607623A2 publication Critical patent/BRPI0607623A2/pt
Publication of BRPI0607623B1 publication Critical patent/BRPI0607623B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D309/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings
    • C07D309/16—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
    • C07D309/28—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D309/30—Oxygen atoms, e.g. delta-lactones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01009—Glucose-6-phosphate isomerase (5.3.1.9)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

CASSETE DE EXPRESSãO DE GENE E UM TRANSFORMANTE, E UM MéTODO PARA PRODUçãO DE 2-DEóXI-SCYLLO-INOSOSE E UM MéTODO PARA PURIFICAR 2-DEóXI-SCYLLO-INOSOSE USANDO REFERIDO TRANSFORMANTE. Um transformante é preparado para inserir pelo menos um cassete de expressão de gene compreendendo um gene envolvido na síntese de 2-deóxi-scyllo-inosose em E. coli como células hospedeiras. Uma 2-deóxi-scyllo-inosose é sintetizada a partir de D-glicose, oligossacarídeo, polissacarídeo, amido e farelo de arroz, usando-se o transformante. Uma solução de cultura contendo a 2-deóxi-scyllo-inosose é tratada com uma coluna tipo leito misturado, ou leito duplo, compreendendo uma forma de hidrogénio de resina de troca de cátion de ácido forte, e uma forma de íon orgânico de resina de troca de ânion básica. A 2-deóxi-scyllo-inosose conforme purificada é reagida com trimetoximetano para converter em 2-deóxi-scyllo-inosose dimetilcetal, e o dimetilacetal é cristalizado e purificado. Em seguida, a DOI é altamente purificada através da hidrolisação do dimetilcetal na presença de ácido.
BRPI0607623-8A 2005-03-30 2006-03-23 escherichia coli transgênica BRPI0607623B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/JP2005/006022 WO2006112000A1 (ja) 2005-03-30 2005-03-30 大腸菌変異株による２－デオキシ－シロ－イノソースの合成および精製する方法並びに得られた２－デオキシ－シロ－イノソース
JPPCT/JP2005/006022 2005-03-30
PCT/JP2006/305782 WO2006109479A1 (ja) 2005-03-30 2006-03-23 遺伝子発現カセット及び形質転換体、並びにこの形質転換体を用いた２－デオキシ－シロ－イノソースの製造方法及び２－デオキシ－シロ－イノソースの精製方法

Publications (2)

Publication Number Publication Date
BRPI0607623A2 true BRPI0607623A2 (pt) 2009-09-22
BRPI0607623B1 BRPI0607623B1 (pt) 2021-03-02

Family

ID=37086767

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0607623-8A BRPI0607623B1 (pt) 2005-03-30 2006-03-23 escherichia coli transgênica

Country Status (8)

Country Link
US (2) US8758741B2 (pt)
EP (1) EP1865056B1 (pt)
JP (1) JP4598826B2 (pt)
KR (1) KR101019759B1 (pt)
CN (1) CN101151368B (pt)
BR (1) BRPI0607623B1 (pt)
DE (1) DE602006019835D1 (pt)
WO (2) WO2006112000A1 (pt)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7521481B2 (en) 2003-02-27 2009-04-21 Mclaurin Joanne Methods of preventing, treating and diagnosing disorders of protein aggregation
JP2010523602A (ja) 2007-04-12 2010-07-15 ワラタ ファーマシューティカルズ， インコーポレイテッド 眼疾患の治療におけるシクロヘキサンヘキソール誘導体の使用
KR101230198B1 (ko) 2008-04-11 2013-02-06 미쓰이 가가쿠 가부시키가이샤 카테콜의 제조 방법
US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
JP5254353B2 (ja) * 2008-11-05 2013-08-07 三井化学株式会社 ２−デオキシ−シロ−イノソース（ｄｏｉ）生産細菌及びこれを用いた２−デオキシ−シロ−イノソース（ｄｏｉ）生産方法
TWI408229B (zh) 2009-03-26 2013-09-11 Asahi Kasei Chemicals Corp 新穎之２－脫氧蟹肌醇合成酶
JP5661250B2 (ja) * 2009-03-26 2015-01-28 旭化成ケミカルズ株式会社 新規グルコース−６−リン酸定量方法および定量試薬
JP5457058B2 (ja) * 2009-03-27 2014-04-02 旭化成ケミカルズ株式会社 リン酸濃度制御による２−デオキシ−シロ−イノソースの製造方法
CN102459139B (zh) 2009-04-28 2014-05-07 三井化学株式会社 多元酚的制备方法
WO2013125666A1 (ja) * 2012-02-23 2013-08-29 株式会社日本触媒 イノシトール高生産微生物およびそれを用いたイノシトールの製造方法
WO2015005451A1 (ja) * 2013-07-12 2015-01-15 三井化学株式会社 ２－デオキシ－シロ－イノソースの生産方法
US10000743B2 (en) 2013-12-16 2018-06-19 Asahi Kasei Chemicals Corporation 2-deoxy-scyllo-inosose reductase
WO2017029353A1 (en) 2015-08-20 2017-02-23 Transition Therapeutics Ireland Limited Treatment of behaviors in dementia patients
KR101718508B1 (ko) * 2015-11-17 2017-03-21 고려대학교 산학협력단 2-데옥시-실로-이노소스 생합성 경로를 가지는 재조합 고초균 및 이를 이용한 2-데옥시-실로-이노소스의 제조방법
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
WO2018180568A1 (ja) 2017-03-27 2018-10-04 学校法人 新潟科学技術学園 変異型２－デオキシ－シロ－イノソース合成酵素
JP7173538B2 (ja) * 2018-10-25 2022-11-16 株式会社Ihi トリヒドロキシベンゼンの製造方法
JP7260872B2 (ja) * 2018-10-25 2023-04-19 株式会社Ihi トリヒドロキシベンゼンを製造するためのシステム

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3122762B2 (ja) * 1999-02-22 2001-01-09 東京工業大学長 ２−デオキシ−シロ−イノソース合成酵素、アミノ酸配列、遺伝子塩基配列
DE10014546A1 (de) * 2000-03-23 2001-09-27 Degussa Für das dapC-Gen kodierende Nukleotidsequenzen und Verfahren zur Herstellung von L-Lysin
JP4019706B2 (ja) * 2000-12-22 2007-12-12 味の素株式会社 発酵法による目的物質の製造法
JP4303526B2 (ja) * 2003-06-11 2009-07-29 北興化学工業株式会社 （−）−２−デオキシ−シロ−イノソースの製造方法

Also Published As

Publication number Publication date
JP4598826B2 (ja) 2010-12-15
US20100015672A1 (en) 2010-01-21
US8758741B2 (en) 2014-06-24
WO2006109479A1 (ja) 2006-10-19
EP1865056A4 (en) 2008-04-16
KR20080005931A (ko) 2008-01-15
EP1865056B1 (en) 2011-01-26
CN101151368A (zh) 2008-03-26
CN101151368B (zh) 2011-06-15
DE602006019835D1 (de) 2011-03-10
BRPI0607623B1 (pt) 2021-03-02
WO2006112000A1 (ja) 2006-10-26
KR101019759B1 (ko) 2011-03-04
JPWO2006109479A1 (ja) 2008-10-23
EP1865056A1 (en) 2007-12-12
US20140256960A1 (en) 2014-09-11

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Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Owner name: NIIGATA BIO-RESEARCH PARK, INC. (JP) , MITSUI CHEM

Free format text: TRANSFERIDO PARTE DOS DIREITOS DE: NIIGATA BIO-RESEARCH PARK, INC.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 02/03/2021, OBSERVADAS AS CONDICOES LEGAIS.