BR112018011193A2 - métodos escalonáveis para produzir vetor viral adeno-associado (aav) recombinante em sistema de cultura celular em suspensão isento de soro adequado para uso clínico - Google Patents

métodos escalonáveis para produzir vetor viral adeno-associado (aav) recombinante em sistema de cultura celular em suspensão isento de soro adequado para uso clínico

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Publication number
BR112018011193A2
BR112018011193A2 BR112018011193A BR112018011193A BR112018011193A2 BR 112018011193 A2 BR112018011193 A2 BR 112018011193A2 BR 112018011193 A BR112018011193 A BR 112018011193A BR 112018011193 A BR112018011193 A BR 112018011193A BR 112018011193 A2 BR112018011193 A2 BR 112018011193A2
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Brazil
Prior art keywords
aav
serum
associated viral
cell culture
viral vector
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BR112018011193A
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English (en)
Inventor
Qu Guang
Fraser Wright John
Lu Lin
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Spark Therapeutics Inc
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Application filed by Spark Therapeutics Inc filed Critical Spark Therapeutics Inc
Publication of BR112018011193A2 publication Critical patent/BR112018011193A2/pt

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Abstract

são divulgados métodos e composições para transfecção de células com plasmídeos. em certas modalidades, são descritos métodos e composições em que a eficiência de transfecção é significativamente aumentada ao contatar as células sendo transduzidas com polietilenimina (pei) que está isenta de ácido nucleico durante o processo de transfecção. vetores virais adeno-associados terapeuticamente úteis gerados de acordo com os métodos e composições divulgados são também divulgados.
BR112018011193A 2015-12-01 2016-12-01 métodos escalonáveis para produzir vetor viral adeno-associado (aav) recombinante em sistema de cultura celular em suspensão isento de soro adequado para uso clínico BR112018011193A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562261815P 2015-12-01 2015-12-01
PCT/US2016/064414 WO2017096039A1 (en) 2015-12-01 2016-12-01 Scalable methods for producing recombinant adeno-associated viral (aav) vector in serum-free suspension cell culture system suitable for clinical use

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BR112018011193A2 true BR112018011193A2 (pt) 2018-11-21

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US (1) US20190292561A1 (pt)
EP (1) EP3384015A4 (pt)
JP (2) JP7444521B2 (pt)
KR (1) KR20180091863A (pt)
CN (1) CN108603174A (pt)
AU (2) AU2016362317B2 (pt)
BR (1) BR112018011193A2 (pt)
CA (1) CA3006309A1 (pt)
CO (1) CO2018006699A2 (pt)
IL (1) IL259595B2 (pt)
MX (2) MX2018006682A (pt)
PE (2) PE20240371A1 (pt)
PH (1) PH12018501168A1 (pt)
RU (1) RU2766583C2 (pt)
SG (2) SG11201804400SA (pt)
WO (1) WO2017096039A1 (pt)

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