MX2023007527A - Metodos y sistemas para transfeccion celular mejorada. - Google Patents

Metodos y sistemas para transfeccion celular mejorada.

Info

Publication number
MX2023007527A
MX2023007527A MX2023007527A MX2023007527A MX2023007527A MX 2023007527 A MX2023007527 A MX 2023007527A MX 2023007527 A MX2023007527 A MX 2023007527A MX 2023007527 A MX2023007527 A MX 2023007527A MX 2023007527 A MX2023007527 A MX 2023007527A
Authority
MX
Mexico
Prior art keywords
systems
methods
cell transfection
improved cell
purposes
Prior art date
Application number
MX2023007527A
Other languages
English (en)
Inventor
Larry Dean Determan
Nathaniel A Jenkins
Daniel Koback
Delaney Kate Kolich
Paul B Lanter
Seyed Pouria Motevalian
Kathryn C Olson
Jeffrey William Pavlicek
Austin Stephen Tritt
Vincent Wingate
Original Assignee
Pfizer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer filed Critical Pfizer
Publication of MX2023007527A publication Critical patent/MX2023007527A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente divulgación proporciona métodos y sistemas mejorados para la transfección de células huésped con ácidos nucleicos, como ADN plasmídico, con el fin de producir de manera eficiente productos biológicos, como vectores AAV, a gran escala.
MX2023007527A 2020-12-21 2021-12-17 Metodos y sistemas para transfeccion celular mejorada. MX2023007527A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063199367P 2020-12-21 2020-12-21
US202163264997P 2021-12-06 2021-12-06
PCT/IB2021/061952 WO2022137061A1 (en) 2020-12-21 2021-12-17 Methods and systems for improved cell transfection

Publications (1)

Publication Number Publication Date
MX2023007527A true MX2023007527A (es) 2023-07-10

Family

ID=79731079

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023007527A MX2023007527A (es) 2020-12-21 2021-12-17 Metodos y sistemas para transfeccion celular mejorada.

Country Status (8)

Country Link
US (1) US20240011048A1 (es)
EP (1) EP4263840A1 (es)
JP (1) JP2024500801A (es)
AU (1) AU2021404929A1 (es)
CA (1) CA3205588A1 (es)
IL (1) IL303920A (es)
MX (1) MX2023007527A (es)
WO (1) WO2022137061A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115386598A (zh) * 2022-09-16 2022-11-25 南京艾尔普再生医学科技有限公司 一种免疫细胞电转液及其制备方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2868099A (en) * 1998-02-13 1999-08-30 Selective Genetics, Inc. Concurrent flow mixing methods and apparatuses for the preparation of gene therapy vectors and compositions prepared thereby
EP1104309B8 (en) * 1998-08-14 2006-07-05 Valentis Inc. Co-lyophilized complex comprising a nucleic acid vector and a formulating agent
WO2015061768A1 (en) * 2013-10-25 2015-04-30 Massachusetts Institute Of Technology High-throughput synthesis of nanoparticles
GB201403684D0 (en) 2014-03-03 2014-04-16 King S College London Vector
CN104974933B (zh) * 2014-04-04 2017-08-15 上海泰因生物技术有限公司 一种大规模连续多次悬浮瞬转表达重组蛋白的装置和方法
US20190000940A1 (en) 2015-07-31 2019-01-03 Voyager Therapeutics, Inc. Compositions and methods for the treatment of aadc deficiency
CA2971303A1 (en) 2016-06-21 2017-12-21 Bamboo Therapeutics, Inc. Optimized mini-dystrophin genes and expression cassettes and their use
WO2018208960A1 (en) * 2017-05-09 2018-11-15 Dimension Therapeutics, Inc. Scalable method for producing transfection reagents

Also Published As

Publication number Publication date
CA3205588A1 (en) 2022-06-30
AU2021404929A1 (en) 2023-07-06
WO2022137061A1 (en) 2022-06-30
JP2024500801A (ja) 2024-01-10
IL303920A (en) 2023-08-01
EP4263840A1 (en) 2023-10-25
US20240011048A1 (en) 2024-01-11

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