BR112013032659A2 - método para o tratamento de células-tronco mesenquimais, que compreende a obtenção e o isolamento das células-tronco mesenquimais e a cultura das células em um meio de tratamento; células-tronco mesenquimais; e uso das células - Google Patents

método para o tratamento de células-tronco mesenquimais, que compreende a obtenção e o isolamento das células-tronco mesenquimais e a cultura das células em um meio de tratamento; células-tronco mesenquimais; e uso das células

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Publication number
BR112013032659A2
BR112013032659A2 BR112013032659A BR112013032659A BR112013032659A2 BR 112013032659 A2 BR112013032659 A2 BR 112013032659A2 BR 112013032659 A BR112013032659 A BR 112013032659A BR 112013032659 A BR112013032659 A BR 112013032659A BR 112013032659 A2 BR112013032659 A2 BR 112013032659A2
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mesenchymal stem
stem cells
cells
treating
isolating
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BR112013032659A
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English (en)
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BR112013032659B1 (pt
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Javier Díez García
María Begoña Castro
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Histocell Sl
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Publication of BR112013032659A2 publication Critical patent/BR112013032659A2/pt
Publication of BR112013032659B1 publication Critical patent/BR112013032659B1/pt

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K33/00—Medicinal preparations containing inorganic active ingredients
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    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
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    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
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    • A61P9/00—Drugs for disorders of the cardiovascular system
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions

Abstract

resumo método para o tratamento de células-tronco mesenquimais, que compreende a obtenção e o isolamento das células-tronco mesenquimais e a cultura das células em um meio de tratamento; células-tronco mesenquimais; e uso das células a presente invenção se refere a um método para o tratamento de células-tronco mesenquimais, preferencialmente de origem adiposa, compreendendo principalmente dois estágios, primeiro a coleta e o isolamento de células-tronco mesenquimais, e segundo, um período de crescimento e de tratamento específico das células em um meio de condicionamento ou tratamento com um agente oxidante. a invenção também compreende as células obtidas diretamente pelo método e seu uso no tratamento de doenças causadas ou associadas ao estresse oxidativo.
BR112013032659-0A 2011-07-06 2011-07-06 Método para o tratamento de células-tronco mesenquimais, que compreende a obtenção e o isolamento das células-tronco mesenquimais e a cultura das células em um meio de tratamento; e uso das células BR112013032659B1 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/ES2011/070489 WO2013004859A1 (es) 2011-07-06 2011-07-06 Método de tratamiento de celulas madre mesenquimales y su uso en el tratamiento de enfermedades asociadas a estrés oxidativo

Publications (2)

Publication Number Publication Date
BR112013032659A2 true BR112013032659A2 (pt) 2017-01-24
BR112013032659B1 BR112013032659B1 (pt) 2021-04-06

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BR112013032659-0A BR112013032659B1 (pt) 2011-07-06 2011-07-06 Método para o tratamento de células-tronco mesenquimais, que compreende a obtenção e o isolamento das células-tronco mesenquimais e a cultura das células em um meio de tratamento; e uso das células

Country Status (13)

Country Link
US (1) US9080153B2 (pt)
EP (1) EP2730650B1 (pt)
JP (1) JP5908582B2 (pt)
KR (1) KR101811481B1 (pt)
CN (1) CN103687940B (pt)
AU (1) AU2011372711B2 (pt)
BR (1) BR112013032659B1 (pt)
CA (1) CA2839106C (pt)
DK (1) DK2730650T3 (pt)
ES (1) ES2620258T3 (pt)
MX (1) MX342200B (pt)
PT (1) PT2730650T (pt)
WO (1) WO2013004859A1 (pt)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2953640B1 (en) 2013-02-07 2020-04-08 The Cleveland Clinic Foundation Nanoencapsulated superoxide dismutase and catalase for treating spinal cord injury
AU2014231766B2 (en) * 2013-03-15 2017-11-09 Close The Loop Technologies Pty Ltd Asphalt including modified toner based additive
CN103789257B (zh) * 2013-09-26 2015-06-03 西南交通大学 一种间充质干细胞的定向分化诱导方法
KR101613478B1 (ko) * 2014-09-22 2016-04-19 (주)안트로젠 중간엽줄기세포-하이드로겔을 함유하는 조성물 및 이의 제조방법
CN104357383A (zh) * 2014-10-11 2015-02-18 张炳强 人脂肪间充质干细胞制备方法及其在制备疾病药物的应用
WO2016107563A1 (zh) * 2014-12-29 2016-07-07 西比曼生物科技（上海）有限公司 一种用于治疗上呼吸道疾病的脂肪间充质祖细胞复合物
JP6992983B2 (ja) * 2015-09-15 2022-01-13 カンステム バイオテック カンパニー リミテッド Ｓｏｄ３を過剰発現する幹細胞を有効成分として含む炎症性疾患の予防又は治療用組成物
JP2018538343A (ja) * 2015-12-22 2018-12-27 サングイ バイオ ピーティーワイ． エルティーディー 赤血球を用いる治療方法
ITUB20159750A1 (it) * 2015-12-30 2017-06-30 Alfredo Gorio Metodo per la promozione ed il miglioramento delle proprieta? del tessuto adiposo, tessuto e cellule ottenute tramite detto metodo
CN106497713B (zh) * 2016-09-14 2019-01-29 深圳海悦生物科技有限公司 可调节皮脂分泌的祛痘皂及其制备方法
CN110366597B (zh) 2016-12-20 2024-03-29 善威生物私人有限公司 用蛋白酶抑制剂进行血液图谱分析
KR102007872B1 (ko) * 2017-06-14 2019-08-06 순천향대학교 산학협력단 줄기세포를 멜라토닌 처리 하여 증식 촉진 또는 생존율을 증가시키는 방법
CN107343957B (zh) * 2017-07-18 2020-04-21 中南大学 一种黑磷/二氧化锰复合纳米材料及其制备方法和应用
CN108888636A (zh) * 2018-08-14 2018-11-27 东营凤起生物科技发展有限公司 一种治疗糖尿病和动脉粥样硬化的方法
CN109486771B (zh) * 2018-12-29 2021-02-12 深圳市旷逸生物科技有限公司 一种高表达sod3的cho细胞株构建方法及sod3蛋白纯化方法
CN109674817B (zh) * 2019-01-31 2021-06-29 哈尔滨医科大学 三氧化二砷在制备治疗晚期动脉粥样硬化药物中的用途
KR20210130578A (ko) 2020-04-22 2021-11-01 주식회사 아바테라퓨틱스 폐섬유증 치료 또는 예방을 위한 세포 치료제 조성물
CN111514164A (zh) * 2020-04-30 2020-08-11 深圳市合一康生物科技股份有限公司 一种用于肺病治疗的脂肪间充质干细胞及其制备方法
KR20220028557A (ko) 2020-08-28 2022-03-08 주식회사 티스템 무막줄기세포추출물의 산화적 스트레스 개선용 조성물
EP3970732A1 (en) * 2020-09-17 2022-03-23 Hunan Yuanpin Cell Technology Co. Ltd Use of mesenchymal stem cells in the treatment of viral infections and/or complications caused by viral infections
WO2023038162A1 (ko) * 2021-09-07 2023-03-16 주식회사 티스템 무막줄기세포추출물의 산화적 스트레스 개선용 조성물
WO2023064404A1 (en) * 2021-10-12 2023-04-20 Mechanogenic K.K. Anti-aging composition using mesenchymal stem cells and methods thereof
CN115317605A (zh) * 2022-08-15 2022-11-11 上海交通大学医学院 一种自主供氧复合光敏剂及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004044142A2 (en) 2002-11-05 2004-05-27 The Brigham And Women's Hospital, Inc. Mesenchymal stem cells and methods of use thereof
WO2007030469A2 (en) * 2005-09-06 2007-03-15 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Transplantable cell growth niche and related compositions and methods
GB2444686B (en) 2005-09-12 2010-08-25 Es Cell Int Pte Ltd Differentiation of pluripotent stem cells using p38 MAPK inhibitors or prostaglandins
CA2723848C (en) * 2008-05-09 2013-01-08 Regenics As Method of making salmon egg cytoplasmic extracts and use for increasing collagen production in skin
WO2009152384A1 (en) * 2008-06-11 2009-12-17 The Children's Mercy Hospital Solutions for tissue engineering and methods of use
US20130164267A1 (en) * 2008-06-11 2013-06-27 Cell4Vet Corporation Adipose tissue-derived stem cells for veterinary use
WO2010011131A1 (en) * 2008-07-25 2010-01-28 Rijksuniversiteit Groningen Oxygen delivering scaffold for tissue engineering
US20100330047A1 (en) 2009-06-26 2010-12-30 Life & Light Ltd. Mesenchymal Stem Cells Grown Under Hypoxic Conditions: Compositions, Methods and Uses Therefor

Also Published As

Publication number Publication date
CA2839106A1 (en) 2013-01-10
CA2839106C (en) 2018-01-02
KR20140148284A (ko) 2014-12-31
WO2013004859A1 (es) 2013-01-10
BR112013032659B1 (pt) 2021-04-06
PT2730650T (pt) 2017-03-23
MX342200B (es) 2016-08-25
AU2011372711B2 (en) 2016-07-14
AU2011372711A1 (en) 2014-01-16
US9080153B2 (en) 2015-07-14
EP2730650A1 (en) 2014-05-14
JP5908582B2 (ja) 2016-04-26
KR101811481B1 (ko) 2017-12-21
JP2014520521A (ja) 2014-08-25
CN103687940B (zh) 2016-10-12
MX2013014307A (es) 2014-05-27
US20140154221A1 (en) 2014-06-05
EP2730650B1 (en) 2017-01-04
ES2620258T3 (es) 2017-06-28
CN103687940A (zh) 2014-03-26
DK2730650T3 (en) 2017-04-10

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