BR112014006244A2 - microrganismos e métodos para produzir alcenos - Google Patents

microrganismos e métodos para produzir alcenos

Info

Publication number
BR112014006244A2
BR112014006244A2 BR112014006244A BR112014006244A BR112014006244A2 BR 112014006244 A2 BR112014006244 A2 BR 112014006244A2 BR 112014006244 A BR112014006244 A BR 112014006244A BR 112014006244 A BR112014006244 A BR 112014006244A BR 112014006244 A2 BR112014006244 A2 BR 112014006244A2
Authority
BR
Brazil
Prior art keywords
alkene
pathway
produce
methods
occurring microbial
Prior art date
Application number
BR112014006244A
Other languages
English (en)
Inventor
J Burk Mark
E Osterhout Robin
Original Assignee
Genomatica Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomatica Inc filed Critical Genomatica Inc
Publication of BR112014006244A2 publication Critical patent/BR112014006244A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1229—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1235—Diphosphotransferases (2.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/002—Preparation of hydrocarbons or halogenated hydrocarbons cyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

abstract the invention provides non-naturally occurring microbial organisms containing an alkene pathway having at least one exogenous nucleic acid encoding an alkene pathway enzyme expressed in a sufficient amount to convert an alcohol to an alkene. the invention additionally provides methods of using such microbial organisms to produce an alkene, by culturing a non-naturally occurring microbial organism containing an alkene pathway as described herein under conditions and for a sufficient period of time to produce an alkene. -------------------------------------------------------------------------- tradução do resumo resumo patente de invenção: "microrganismos e métodos para produzir alcenos". a invenção provê organismos microbianos de ocorrência não na-tural contendo uma via de alceno tendo pelo menos um ácido nucleico exó-geno codificando uma enzima de via de alceno expressa em uma quantia suficiente para converter um álcool em um alceno. a invenção adicionalmente provê métodos para uso de tais organismos microbianos para produzir um alceno, através da cultura de um organismo microbiano de ocorrência não natural contendo uma via de alceno como descrita aqui sob condições e por um período de tempo suficiente para produzir um alceno.
BR112014006244A 2011-09-16 2012-09-14 microrganismos e métodos para produzir alcenos BR112014006244A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161535893P 2011-09-16 2011-09-16
PCT/US2012/055469 WO2013040383A1 (en) 2011-09-16 2012-09-14 Microorganisms and methods for producing alkenes

Publications (1)

Publication Number Publication Date
BR112014006244A2 true BR112014006244A2 (pt) 2017-04-11

Family

ID=47883778

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014006244A BR112014006244A2 (pt) 2011-09-16 2012-09-14 microrganismos e métodos para produzir alcenos

Country Status (11)

Country Link
US (2) US9988648B2 (pt)
EP (1) EP2756082A4 (pt)
KR (1) KR20140070605A (pt)
CN (1) CN103946383A (pt)
AU (1) AU2012308369A1 (pt)
BR (1) BR112014006244A2 (pt)
CA (1) CA2848972A1 (pt)
CO (1) CO7020865A2 (pt)
MX (1) MX2014003212A (pt)
SG (2) SG11201400638VA (pt)
WO (1) WO2013040383A1 (pt)

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CA2876484A1 (en) 2012-06-15 2013-12-19 Microvi Biotech Inc. Novel biocatalyst compositions and processes for use
US9453244B2 (en) 2012-06-29 2016-09-27 Global Bioenergies Method for producing a monoalkene by enzymatic conversion of an alkyl monoester
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WO2014085612A1 (en) 2012-11-28 2014-06-05 INVISTA North America S.á r.l. Methods for biosynthesis of isobutene
WO2014099927A1 (en) * 2012-12-17 2014-06-26 Braskem S/A Ap 09 Modified microorganisms and methods of using same for producing isoprene, 2-methyl-1-butanol, 2-methyl-1,3-butanediol, and/or 2-methylbut-2-en-1-ol
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BR112019000633A2 (pt) * 2016-07-12 2019-07-09 Braskem Sa formação de alquenos através de desidratação enzimática de alcanóis
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Also Published As

Publication number Publication date
WO2013040383A1 (en) 2013-03-21
US11932893B2 (en) 2024-03-19
MX2014003212A (es) 2015-03-19
SG10201502101WA (en) 2015-05-28
US20190085360A1 (en) 2019-03-21
CN103946383A (zh) 2014-07-23
SG11201400638VA (en) 2014-04-28
KR20140070605A (ko) 2014-06-10
US9988648B2 (en) 2018-06-05
EP2756082A1 (en) 2014-07-23
US20130122563A1 (en) 2013-05-16
EP2756082A4 (en) 2015-10-28
NZ622451A (en) 2016-05-27
AU2012308369A1 (en) 2014-04-03
CA2848972A1 (en) 2013-03-21
CO7020865A2 (es) 2014-08-11

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Legal Events

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]