BR112016002526A2 - método para produção de um composto heterólogo não catabólico e um isoprenoide heterólogo, e, composição de fermentação - Google Patents
método para produção de um composto heterólogo não catabólico e um isoprenoide heterólogo, e, composição de fermentaçãoInfo
- Publication number
- BR112016002526A2 BR112016002526A2 BR112016002526A BR112016002526A BR112016002526A2 BR 112016002526 A2 BR112016002526 A2 BR 112016002526A2 BR 112016002526 A BR112016002526 A BR 112016002526A BR 112016002526 A BR112016002526 A BR 112016002526A BR 112016002526 A2 BR112016002526 A2 BR 112016002526A2
- Authority
- BR
- Brazil
- Prior art keywords
- catabolic
- heterologous
- genetically modified
- producing
- compounds
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
a presente descrição refere-se ao uso de um comutador para a produção de compostos heterólogos não catabólicos em células hospedeiras microbianas. em um aspecto, são providos aqui micro-organismos geneticamente modificados que produzem compostos não catabólicos mais estavelmente quando serialmente cultivados sob condições aeróbicas seguidas por condições microaeróbicas, e métodos para produção de compostos não catabólicos pelo cultivo de micróbios geneticamente modificados sob tais condições de cultivo. em um outro aspecto, são aqui providos micro-organismos geneticamente modificados que produzem compostos não catabólicos mais estavelmente quando serialmente cultivados na presença de maltose seguidos pela redução ou ausência de maltose, e métodos para produção de compostos não catabólicos pelo cultivo dos micróbios geneticamente modificados sob tais condições de cultivo.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US2013/054028 WO2015020649A1 (en) | 2013-08-07 | 2013-08-07 | Methods for stabilizing production of acetyl-coenzyme a derived compounds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112016002526A2 true BR112016002526A2 (pt) | 2018-03-27 |
| BR112016002526B1 BR112016002526B1 (pt) | 2021-11-23 |
Family
ID=48980380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112016002526-1A BR112016002526B1 (pt) | 2013-08-07 | 2013-08-07 | Método para produção de um composto heterólogo não catabólico, e, composição de fermentação |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10563229B2 (pt) |
| EP (2) | EP3663392A1 (pt) |
| CN (1) | CN105934517A (pt) |
| AU (1) | AU2013397496B2 (pt) |
| BR (1) | BR112016002526B1 (pt) |
| CA (1) | CA2918891C (pt) |
| PT (1) | PT3030662T (pt) |
| WO (1) | WO2015020649A1 (pt) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10704050B2 (en) | 2013-10-18 | 2020-07-07 | Biopetrolia Ab | Engineering of acetyl-CoA metabolism in yeast |
| CN108474009B (zh) | 2015-06-25 | 2022-10-04 | 阿迈瑞斯公司 | 麦芽糖依赖性降解决定子、麦芽糖响应型启动子、稳定化构建体及其在生成非分解代谢化合物中的用途 |
| CN109311931A (zh) * | 2016-04-07 | 2019-02-05 | 何塞·L·阿瓦洛斯 | 用于生产化学物质的光基因控制代谢途径的系统和方法 |
| ES2906310T3 (es) | 2016-08-12 | 2022-04-18 | Amyris Inc | Glicosiltransferasa dependiente de UDP para la producción de alta eficiencia de rebaudiósidos |
| US11946087B2 (en) | 2017-01-25 | 2024-04-02 | Amyris Bio Products Portugal, Unipessoal, Lda | Co-production of a sesquiterpene and a carotenoid |
| JP7487099B2 (ja) | 2017-08-11 | 2024-05-20 | アミリス， インコーポレイテッド | レバウジオシドの高効率生成のためのエンドウ（ｐｉｓｕｍ ｓａｔｉｖｕｍ）カウレンオキシダーゼ |
| EP3720944A1 (en) | 2017-12-07 | 2020-10-14 | Zymergen Inc. | Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation |
| CN111868047A (zh) | 2017-12-21 | 2020-10-30 | 齐默尔根公司 | 荆芥内半缩醛氧化还原酶、荆芥内半缩醛合酶和能够产生荆芥内酯的微生物 |
| MX2021000053A (es) | 2018-07-12 | 2021-03-25 | Amyris Inc | Metodos para controlar velocidades de alimentacion de la fermentacion. |
| WO2020081468A1 (en) | 2018-10-15 | 2020-04-23 | Amyris, Inc. | Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides |
| US10519475B1 (en) | 2018-11-21 | 2019-12-31 | Amyris, Inc. | Biosynthesis of compounds in yeast |
| US20220106619A1 (en) | 2019-01-24 | 2022-04-07 | Amyris, Inc. | Abc transporters for the high efficiency production of rebaudiosides |
| US20220127620A1 (en) | 2019-03-15 | 2022-04-28 | Andrew P. Klein | Microbial production of compounds |
| CN111826357B (zh) * | 2019-04-15 | 2021-11-19 | 中国科学院分子植物科学卓越创新中心 | 三分三非典型iii型聚酮合酶及其应用 |
| MX2022001073A (es) | 2019-08-01 | 2022-02-14 | Amyris Inc | Celulas hospederas modificadas para la produccion de vanillina de alta eficiencia. |
| CA3193475A1 (en) | 2020-09-15 | 2022-03-24 | Amyris, Inc. | Culture compositions and methods of their use for high yield production of vanillin |
| US20240175066A1 (en) | 2021-03-19 | 2024-05-30 | Amyris , Inc. | Modified host cells for high efficiency production of vanillin |
| WO2023230411A1 (en) | 2022-05-26 | 2023-11-30 | Amyris, Inc. | Improved oligosaccharide production in yeast |
| WO2023244843A1 (en) | 2022-06-16 | 2023-12-21 | Amyris, Inc. | HIGH pH METHODS AND COMPOSITIONS FOR CULTURING GENETICALLY MODIFIED HOST CELLS |
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| FR8F (fr) | 1960-07-11 | 1962-02-16 | Laboratoire A Castaigne | Applications thérapeutiques de l'arginase corps 45-25c. |
| US4703009A (en) | 1983-03-08 | 1987-10-27 | Merck & Co., Inc. | RDNA cloning vector pVE1, deletion and hybrid mutants and recombinant derivatives thereof products and processes |
| DE3477936D1 (en) | 1983-03-08 | 1989-06-01 | Merck & Co Inc | Recombinant dna cloning vector pve1, deletion and hybrid mutants, and recombinant derivatives thereof, products and processes |
| US5272065A (en) | 1983-10-20 | 1993-12-21 | Research Foundation Of State University Of New York | Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA |
| IL81892A (en) | 1986-03-21 | 1995-10-31 | Lilly Co Eli | Recombinant AND molecule containing a sequence encoding O-methyltransfer macrocin |
| US5672497A (en) | 1986-03-21 | 1997-09-30 | Eli Lilly And Company | Method for increasing the antibiotic-producing ability of antibiotic-producing microorganisms |
| US5098837A (en) | 1988-06-07 | 1992-03-24 | Eli Lilly And Company | Macrolide biosynthetic genes for use in streptomyces and other organisms |
| US5149638A (en) | 1988-09-29 | 1992-09-22 | Eli Lilly And Company | Tylosin biosynthetic genes tylA, tylB and tylI |
| US5252474A (en) | 1989-03-31 | 1993-10-12 | Merck & Co., Inc. | Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use |
| US6060234A (en) | 1991-01-17 | 2000-05-09 | Abbott Laboratories | Polyketide derivatives and recombinant methods for making same |
| US5824513A (en) | 1991-01-17 | 1998-10-20 | Abbott Laboratories | Recombinant DNA method for producing erythromycin analogs |
| WO1993013663A1 (en) | 1992-01-17 | 1993-07-22 | Abbott Laboratories | Method of directing biosynthesis of specific polyketides |
| CA2175461A1 (en) | 1993-11-02 | 1995-05-11 | Victor A. Vinci | Dna encoding triol polyketide synthase |
| US5716849A (en) | 1994-06-08 | 1998-02-10 | Novartis Finance Corporation | Genes for the biosynthesis of soraphen |
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| CA2197524A1 (en) | 1996-02-22 | 1997-08-22 | Bradley Stuart Dehoff | Polyketide synthase genes |
| CA2197160C (en) | 1996-02-22 | 2007-05-01 | Stanley Gene Burgett | Platenolide synthase gene |
| DE69736673T2 (de) | 1996-08-20 | 2007-09-13 | Novartis Ag | Rifamycin biosynthese genkluster |
| WO1999005283A2 (fr) | 1997-07-25 | 1999-02-04 | Hoechst Marion Roussel | Genes de biosynthese et de transfert des 6-desoxyhexoses chez saccharopolyspora erythraea et chez streptomyces antibioticus et leur utilisation |
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| JP2009521936A (ja) | 2006-01-03 | 2009-06-11 | マサチューセッツ・インスティチュート・オブ・テクノロジー | プロモータ工学及び遺伝子制御 |
| MX302107B (es) * | 2007-09-20 | 2012-08-08 | Amyris Biotechnologies Inc | Produccion de isoprenoides. |
| US20120196339A1 (en) * | 2011-01-31 | 2012-08-02 | Los Alamos National Security Llc | Production of industrially relevant compounds in prokaryotic organisms |
| CN108474009B (zh) * | 2015-06-25 | 2022-10-04 | 阿迈瑞斯公司 | 麦芽糖依赖性降解决定子、麦芽糖响应型启动子、稳定化构建体及其在生成非分解代谢化合物中的用途 |
-
2013
- 2013-08-07 BR BR112016002526-1A patent/BR112016002526B1/pt active IP Right Grant
- 2013-08-07 EP EP20151280.3A patent/EP3663392A1/en not_active Withdrawn
- 2013-08-07 PT PT137482550T patent/PT3030662T/pt unknown
- 2013-08-07 WO PCT/US2013/054028 patent/WO2015020649A1/en active Application Filing
- 2013-08-07 CA CA2918891A patent/CA2918891C/en active Active
- 2013-08-07 EP EP13748255.0A patent/EP3030662B1/en active Active
- 2013-08-07 AU AU2013397496A patent/AU2013397496B2/en active Active
- 2013-08-07 US US14/910,240 patent/US10563229B2/en active Active
- 2013-08-07 CN CN201380080077.8A patent/CN105934517A/zh active Pending
-
2020
- 2020-01-02 US US16/733,191 patent/US11230716B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| AU2013397496A1 (en) | 2016-02-18 |
| PT3030662T (pt) | 2020-03-25 |
| AU2013397496B2 (en) | 2020-03-12 |
| EP3663392A1 (en) | 2020-06-10 |
| US20160177341A1 (en) | 2016-06-23 |
| CA2918891A1 (en) | 2015-02-12 |
| WO2015020649A1 (en) | 2015-02-12 |
| CN105934517A (zh) | 2016-09-07 |
| US11230716B2 (en) | 2022-01-25 |
| CA2918891C (en) | 2023-03-14 |
| US10563229B2 (en) | 2020-02-18 |
| BR112016002526B1 (pt) | 2021-11-23 |
| US20200270643A1 (en) | 2020-08-27 |
| EP3030662A1 (en) | 2016-06-15 |
| EP3030662B1 (en) | 2020-01-15 |
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Legal Events
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| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 07/08/2013, OBSERVADAS AS CONDICOES LEGAIS. |