BR112017003556A2 - método de cultivo de um micro-organismo. - Google Patents
método de cultivo de um micro-organismo.Info
- Publication number
- BR112017003556A2 BR112017003556A2 BR112017003556-1A BR112017003556A BR112017003556A2 BR 112017003556 A2 BR112017003556 A2 BR 112017003556A2 BR 112017003556 A BR112017003556 A BR 112017003556A BR 112017003556 A2 BR112017003556 A2 BR 112017003556A2
- Authority
- BR
- Brazil
- Prior art keywords
- container
- microorganism
- microorganisms
- crop
- carbon
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6431—Linoleic acids [18:2[n-6]]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6432—Eicosapentaenoic acids [EPA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6434—Docosahexenoic acids [DHA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
são fornecidos neste documento métodos de cultivo de um micro-organismo. os métodos incluem o fornecimento de um recipiente compreendendo um ou mais micro-organismos em um meio, que tem uma primeira razão de carbono e nitrogênio; cultura de micro-organismos até que a cultura atinja um indicador limite; colheita de uma parte da cultura, mantendo a maior parte da cultura no recipiente; e adição de meio fresco contendo uma segunda razão de carbono e nitrogênio ao recipiente com a maior parte da cultura que consiste nos micro-organismos.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462064668P | 2014-10-16 | 2014-10-16 | |
US62/064668 | 2014-10-16 | ||
US62/064,668 | 2014-10-16 | ||
PCT/IB2015/057807 WO2016059540A1 (en) | 2014-10-16 | 2015-10-12 | Semi-continuous culture methods |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112017003556A2 true BR112017003556A2 (pt) | 2018-02-27 |
BR112017003556B1 BR112017003556B1 (pt) | 2023-10-03 |
Family
ID=55746207
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112017003556-1A BR112017003556B1 (pt) | 2014-10-16 | 2015-10-12 | Método em batelada alimentada repetida de cultivo de microorganismos thraustochytrid |
Country Status (16)
Country | Link |
---|---|
US (3) | US10676775B2 (pt) |
EP (2) | EP3207164B1 (pt) |
JP (1) | JP6806297B2 (pt) |
KR (1) | KR102501031B1 (pt) |
CN (1) | CN106687605B (pt) |
AU (3) | AU2015332094B2 (pt) |
BR (1) | BR112017003556B1 (pt) |
CA (1) | CA2959860C (pt) |
CL (1) | CL2017000891A1 (pt) |
DK (1) | DK3207164T3 (pt) |
ES (1) | ES2856838T3 (pt) |
IL (1) | IL250275A0 (pt) |
MX (1) | MX2017002234A (pt) |
NZ (1) | NZ728653A (pt) |
PT (1) | PT3207164T (pt) |
WO (1) | WO2016059540A1 (pt) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10851395B2 (en) | 2016-06-10 | 2020-12-01 | MARA Renewables Corporation | Method of making lipids with improved cold flow properties |
KR101956440B1 (ko) * | 2017-02-10 | 2019-06-24 | 부경대학교 산학협력단 | 반연속 배양 방법을 이용한 바이러스의 생산 방법 |
CN107805649A (zh) | 2017-11-10 | 2018-03-16 | 浙江帝斯曼中肯生物科技有限公司 | 一种生产结冷胶的新型发酵工艺 |
CN109371071B (zh) * | 2018-05-17 | 2022-11-04 | 梁云 | Dha微生物油脂中脂肪酸组合物成分调整的方法 |
CN109136308B (zh) * | 2018-09-26 | 2021-01-26 | 武汉中科光谷绿色生物技术有限公司 | 一种提高发酵产聚唾液酸的方法及发酵液 |
CN109161570B (zh) * | 2018-09-26 | 2020-12-18 | 武汉中科光谷绿色生物技术有限公司 | 一种提高发酵产n-乙酰神经氨酸的方法及发酵液 |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5340742A (en) | 1988-09-07 | 1994-08-23 | Omegatech Inc. | Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids |
US5340594A (en) | 1988-09-07 | 1994-08-23 | Omegatech Inc. | Food product having high concentrations of omega-3 highly unsaturated fatty acids |
US5130242A (en) | 1988-09-07 | 1992-07-14 | Phycotech, Inc. | Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids |
DE60130737T3 (de) | 2000-01-28 | 2016-01-14 | Dsm Ip Assets B.V. | Verstärkte Produktion von Lipiden enthaltend mehrfachungesättigte Fettsäuren durch hochdichte Kulturen von eukariotischen Mikroben in Gärvorrichtungen |
US7314209B2 (en) | 2004-08-06 | 2008-01-01 | Cooper Standard Automotive, Inc. | Stub out fluid quick connector with shut off valve interface |
ES2576986T3 (es) * | 2005-06-07 | 2016-07-12 | Dsm Nutritional Products Ag | Microorganismos eucariotas para la producción de lípidos y antioxidantes |
JP5531324B2 (ja) * | 2006-08-01 | 2014-06-25 | ディーエスエム ニュートリショナル プロダクツ アーゲー | 油生産微生物およびその改変方法 |
US20090064567A1 (en) * | 2007-09-12 | 2009-03-12 | Martek Biosciences Corporation | Biological oils and production and uses Thereof |
WO2009094440A1 (en) * | 2008-01-25 | 2009-07-30 | Aquatic Energy Llc | Algal culture production, harvesting, and processing |
EP2321404A4 (en) * | 2008-08-04 | 2012-03-21 | Kai Bioenergy | CONTINUOUS CULTIVATION, HARVEST AND OIL TRACTION PHOTOSYNTHETIC CULTURES |
EP2401386A4 (en) * | 2009-02-25 | 2013-03-13 | Vb Medicare Pvt Ltd | IMPROVED METHODS FOR THE FERMENTATIVE MANUFACTURE OF DOCOSAHEXAENIC ACID |
AU2011262568B2 (en) * | 2010-06-09 | 2015-01-29 | Fermentalg | Compositions comprising eicosapentaenoic acid suitable for high purification |
MX338479B (es) | 2011-03-07 | 2016-04-19 | Dsm Nutritional Products Ag | Microorganismos thraustochytrid modificados. |
WO2013088407A1 (en) * | 2011-12-14 | 2013-06-20 | CO.MA.SE. S.r.l. | Process for production of algal biomass |
CN102888348B (zh) * | 2012-07-12 | 2014-12-10 | 中国科学院青岛生物能源与过程研究所 | 一种裂殖壶菌及利用其高密度发酵生产dha油脂的方法 |
-
2015
- 2015-10-12 NZ NZ728653A patent/NZ728653A/en unknown
- 2015-10-12 JP JP2017513131A patent/JP6806297B2/ja active Active
- 2015-10-12 CA CA2959860A patent/CA2959860C/en active Active
- 2015-10-12 KR KR1020177008994A patent/KR102501031B1/ko active IP Right Grant
- 2015-10-12 DK DK15850279.9T patent/DK3207164T3/da active
- 2015-10-12 MX MX2017002234A patent/MX2017002234A/es unknown
- 2015-10-12 WO PCT/IB2015/057807 patent/WO2016059540A1/en active Application Filing
- 2015-10-12 AU AU2015332094A patent/AU2015332094B2/en active Active
- 2015-10-12 EP EP15850279.9A patent/EP3207164B1/en active Active
- 2015-10-12 EP EP21152366.7A patent/EP3839041A1/en active Pending
- 2015-10-12 ES ES15850279T patent/ES2856838T3/es active Active
- 2015-10-12 CN CN201580045740.XA patent/CN106687605B/zh active Active
- 2015-10-12 BR BR112017003556-1A patent/BR112017003556B1/pt active IP Right Grant
- 2015-10-12 PT PT158502799T patent/PT3207164T/pt unknown
- 2015-10-14 US US14/882,734 patent/US10676775B2/en active Active
-
2017
- 2017-01-25 IL IL250275A patent/IL250275A0/en unknown
- 2017-04-11 CL CL2017000891A patent/CL2017000891A1/es unknown
-
2020
- 2020-04-30 US US16/864,128 patent/US11345943B2/en active Active
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2021
- 2021-08-16 AU AU2021218000A patent/AU2021218000B2/en active Active
-
2022
- 2022-04-25 US US17/728,833 patent/US20220356506A1/en active Pending
-
2023
- 2023-08-24 AU AU2023219925A patent/AU2023219925A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
AU2021218000A1 (en) | 2021-09-02 |
CA2959860C (en) | 2023-04-18 |
IL250275A0 (en) | 2017-03-30 |
EP3207164B1 (en) | 2021-01-20 |
BR112017003556B1 (pt) | 2023-10-03 |
AU2021218000B2 (en) | 2023-06-15 |
JP2017530699A (ja) | 2017-10-19 |
US20200332338A1 (en) | 2020-10-22 |
CA2959860A1 (en) | 2016-04-21 |
MX2017002234A (es) | 2017-09-15 |
AU2015332094A1 (en) | 2017-02-16 |
US11345943B2 (en) | 2022-05-31 |
KR20170068461A (ko) | 2017-06-19 |
US10676775B2 (en) | 2020-06-09 |
CL2017000891A1 (es) | 2017-11-03 |
CN106687605A (zh) | 2017-05-17 |
US20220356506A1 (en) | 2022-11-10 |
DK3207164T3 (da) | 2021-02-08 |
WO2016059540A1 (en) | 2016-04-21 |
PT3207164T (pt) | 2021-02-05 |
ES2856838T3 (es) | 2021-09-28 |
AU2023219925A1 (en) | 2023-09-14 |
CN106687605B (zh) | 2022-02-01 |
EP3207164A1 (en) | 2017-08-23 |
US20160108452A1 (en) | 2016-04-21 |
EP3207164A4 (en) | 2018-04-18 |
AU2015332094B2 (en) | 2021-05-20 |
JP6806297B2 (ja) | 2021-01-06 |
NZ728653A (en) | 2023-07-28 |
KR102501031B1 (ko) | 2023-02-16 |
EP3839041A1 (en) | 2021-06-23 |
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Legal Events
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B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 12/10/2015, OBSERVADAS AS CONDICOES LEGAIS |