DK3207164T3 - Halvkontinuerlige dyrkningsfremgangsmåder - Google Patents

Halvkontinuerlige dyrkningsfremgangsmåder Download PDF

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Publication number
DK3207164T3
DK3207164T3 DK15850279.9T DK15850279T DK3207164T3 DK 3207164 T3 DK3207164 T3 DK 3207164T3 DK 15850279 T DK15850279 T DK 15850279T DK 3207164 T3 DK3207164 T3 DK 3207164T3
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DK
Denmark
Prior art keywords
semi
continuous cultivation
cultivation procedures
procedures
continuous
Prior art date
Application number
DK15850279.9T
Other languages
English (en)
Inventor
Zhiyong Sun
Michael Milway
Kevin Berryman
Mercia Valentine
Roberto E Armenta
Laura Purdue
Original Assignee
Mara Renewables Corp
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Publication of DK3207164T3 publication Critical patent/DK3207164T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04Preserving or maintaining viable microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12Unicellular algae; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • C12P7/6427Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • C12P7/6427Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431Linoleic acids [18:2[n-6]]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • C12P7/6427Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432Eicosapentaenoic acids [EPA]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • C12P7/6427Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434Docosahexenoic acids [DHA]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • C12P7/6445Glycerides
    • C12P7/6463Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • C12P7/6445Glycerides
    • C12P7/6472Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06Quantitative determination
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK15850279.9T 2014-10-16 2015-10-12 Halvkontinuerlige dyrkningsfremgangsmåder DK3207164T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462064668P 2014-10-16 2014-10-16
PCT/IB2015/057807 WO2016059540A1 (en) 2014-10-16 2015-10-12 Semi-continuous culture methods

Publications (1)

Publication Number Publication Date
DK3207164T3 true DK3207164T3 (da) 2021-02-08

Family

ID=55746207

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15850279.9T DK3207164T3 (da) 2014-10-16 2015-10-12 Halvkontinuerlige dyrkningsfremgangsmåder

Country Status (16)

Country Link
US (3) US10676775B2 (da)
EP (2) EP3207164B1 (da)
JP (1) JP6806297B2 (da)
KR (1) KR102501031B1 (da)
CN (1) CN106687605B (da)
AU (3) AU2015332094B2 (da)
BR (1) BR112017003556B1 (da)
CA (1) CA2959860C (da)
CL (1) CL2017000891A1 (da)
DK (1) DK3207164T3 (da)
ES (1) ES2856838T3 (da)
IL (1) IL250275A0 (da)
MX (1) MX2017002234A (da)
NZ (1) NZ728653A (da)
PT (1) PT3207164T (da)
WO (1) WO2016059540A1 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10851395B2 (en) * 2016-06-10 2020-12-01 MARA Renewables Corporation Method of making lipids with improved cold flow properties
KR101956440B1 (ko) * 2017-02-10 2019-06-24 부경대학교 산학협력단 반연속 배양 방법을 이용한 바이러스의 생산 방법
CN107805649A (zh) 2017-11-10 2018-03-16 浙江帝斯曼中肯生物科技有限公司 一种生产结冷胶的新型发酵工艺
CN109371071B (zh) * 2018-05-17 2022-11-04 梁云 Dha微生物油脂中脂肪酸组合物成分调整的方法
CN109161570B (zh) * 2018-09-26 2020-12-18 武汉中科光谷绿色生物技术有限公司 一种提高发酵产n-乙酰神经氨酸的方法及发酵液
CN109136308B (zh) * 2018-09-26 2021-01-26 武汉中科光谷绿色生物技术有限公司 一种提高发酵产聚唾液酸的方法及发酵液

Family Cites Families (15)

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US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5130242A (en) 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340594A (en) 1988-09-07 1994-08-23 Omegatech Inc. Food product having high concentrations of omega-3 highly unsaturated fatty acids
CA2396691C (en) 2000-01-28 2012-09-18 Omegatech, Inc. Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors
US7314209B2 (en) 2004-08-06 2008-01-01 Cooper Standard Automotive, Inc. Stub out fluid quick connector with shut off valve interface
ES2909600T3 (es) 2005-06-07 2022-05-09 Dsm Nutritional Products Ag Microorganismos eucarióticos para producir lípidos y antioxidantes
MX2009001346A (es) 2006-08-01 2009-04-17 Ocean Nutrition Canada Ltd Microbios productores de aceite y metodos de modificacion de los mismos.
CN113846129A (zh) * 2007-09-12 2021-12-28 帝斯曼知识产权资产管理有限公司 生物油及其制备与应用
KR20100120660A (ko) * 2008-01-25 2010-11-16 아쿠아틱 에너지 엘엘씨 조류 배양물 생산, 수확 및 가공
BRPI0917961A2 (pt) * 2008-08-04 2017-04-11 Kai Bioenergy cultivo, colheita e extração contínua de óleo de culturas fotossintéticas
EP2401386A4 (en) * 2009-02-25 2013-03-13 Vb Medicare Pvt Ltd IMPROVED METHODS FOR THE FERMENTATIVE MANUFACTURE OF DOCOSAHEXAENIC ACID
ES2733134T3 (es) * 2010-06-09 2019-11-27 Fermentalg Composiciones que comprenden ácido eicosapentaenoico adecuado para una alta purificación
CA2829296C (en) 2011-03-07 2019-10-29 ShuoCheng ZHANG Engineering thraustochytrid microorganisms
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CN102888348B (zh) * 2012-07-12 2014-12-10 中国科学院青岛生物能源与过程研究所 一种裂殖壶菌及利用其高密度发酵生产dha油脂的方法

Also Published As

Publication number Publication date
AU2015332094B2 (en) 2021-05-20
ES2856838T3 (es) 2021-09-28
CA2959860A1 (en) 2016-04-21
EP3207164A4 (en) 2018-04-18
EP3207164B1 (en) 2021-01-20
CN106687605B (zh) 2022-02-01
KR102501031B1 (ko) 2023-02-16
CL2017000891A1 (es) 2017-11-03
PT3207164T (pt) 2021-02-05
US20200332338A1 (en) 2020-10-22
NZ728653A (en) 2023-07-28
KR20170068461A (ko) 2017-06-19
AU2021218000B2 (en) 2023-06-15
BR112017003556B1 (pt) 2023-10-03
US10676775B2 (en) 2020-06-09
BR112017003556A2 (pt) 2018-02-27
CA2959860C (en) 2023-04-18
MX2017002234A (es) 2017-09-15
IL250275A0 (en) 2017-03-30
AU2015332094A1 (en) 2017-02-16
CN106687605A (zh) 2017-05-17
AU2021218000A1 (en) 2021-09-02
AU2023219925A1 (en) 2023-09-14
EP3207164A1 (en) 2017-08-23
US20160108452A1 (en) 2016-04-21
US11345943B2 (en) 2022-05-31
EP3839041A1 (en) 2021-06-23
JP2017530699A (ja) 2017-10-19
US20220356506A1 (en) 2022-11-10
WO2016059540A1 (en) 2016-04-21
JP6806297B2 (ja) 2021-01-06

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