MX338479B - Microorganismos thraustochytrid modificados. - Google Patents

Microorganismos thraustochytrid modificados.

Info

Publication number
MX338479B
MX338479B MX2013010167A MX2013010167A MX338479B MX 338479 B MX338479 B MX 338479B MX 2013010167 A MX2013010167 A MX 2013010167A MX 2013010167 A MX2013010167 A MX 2013010167A MX 338479 B MX338479 B MX 338479B
Authority
MX
Mexico
Prior art keywords
microorganisms
engineering
thraustochytrium
thraustochytrid
methods
Prior art date
Application number
MX2013010167A
Other languages
English (en)
Other versions
MX2013010167A (es
Inventor
Shuocheng Zhang
Roberto Armenta
Original Assignee
Dsm Nutritional Products Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Nutritional Products Ag filed Critical Dsm Nutritional Products Ag
Publication of MX2013010167A publication Critical patent/MX2013010167A/es
Publication of MX338479B publication Critical patent/MX338479B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a inter alia, Thraustochytrium y métodos y reactivos relevantes, que incluyen secuencias reguladoras modificadas de y/o operativas en Thraustochytrid o Thraustochytrium, marcadores seleccionables útiles para modificar microrganismos tales como Thraustochytrids, medios para mutagenizar microrganismos, nuevas cepas producidas por mutagénesis, y métodos y composiciones relacionadas con la producción de compuestos particulares en microrganismos. Un método para transformar una célula de Thraustochytrium, caracterizado porque comprende las etapas de: (a) proporcionar una célula de Thraustochytrium competente; (b) suministrar un ácido nucleico recombinante dentro de la célula competente de Thraustochytrium, en donde el ácido nucleico recombinante comprende un marcador seleccionable y un promotor de tubulina que es por lo menos 80% idéntico a la SEC ID NO: 10; y (c) cultivar la célula competente de Thraustochytrium en un medio de cultivo que contiene un agente de selección que reduce el crecimiento de células sin el marcador seleccionable.
MX2013010167A 2011-03-07 2012-03-07 Microorganismos thraustochytrid modificados. MX338479B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161449848P 2011-03-07 2011-03-07
PCT/IB2012/000528 WO2012120375A1 (en) 2011-03-07 2012-03-07 Engineering thraustochytrid microorganisms

Publications (2)

Publication Number Publication Date
MX2013010167A MX2013010167A (es) 2014-04-14
MX338479B true MX338479B (es) 2016-04-19

Family

ID=46797543

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013010167A MX338479B (es) 2011-03-07 2012-03-07 Microorganismos thraustochytrid modificados.

Country Status (12)

Country Link
US (2) US9133463B2 (es)
EP (1) EP2683824B1 (es)
JP (1) JP6168700B2 (es)
CN (2) CN107630017A (es)
AU (1) AU2012226528B2 (es)
CA (1) CA2829296C (es)
DK (1) DK2683824T3 (es)
ES (1) ES2678997T3 (es)
MX (1) MX338479B (es)
NO (1) NO2773848T3 (es)
PT (1) PT2683824T (es)
WO (1) WO2012120375A1 (es)

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Also Published As

Publication number Publication date
DK2683824T3 (en) 2018-06-18
JP6168700B2 (ja) 2017-07-26
CA2829296A1 (en) 2012-09-13
CA2829296C (en) 2019-10-29
AU2012226528A1 (en) 2013-05-02
EP2683824A4 (en) 2015-05-06
US9133463B2 (en) 2015-09-15
EP2683824B1 (en) 2018-04-25
US20120244584A1 (en) 2012-09-27
US20160010095A1 (en) 2016-01-14
CN103649313B (zh) 2017-10-24
JP2014507952A (ja) 2014-04-03
NO2773848T3 (es) 2018-03-31
WO2012120375A1 (en) 2012-09-13
CN107630017A (zh) 2018-01-26
EP2683824A1 (en) 2014-01-15
PT2683824T (pt) 2018-06-07
CN103649313A (zh) 2014-03-19
ES2678997T3 (es) 2018-08-21
US9758787B2 (en) 2017-09-12
AU2012226528B2 (en) 2015-08-20
MX2013010167A (es) 2014-04-14

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