MX2013010167A - Microorganismos thraustochytrid modificados. - Google Patents

Microorganismos thraustochytrid modificados.

Info

Publication number
MX2013010167A
MX2013010167A MX2013010167A MX2013010167A MX2013010167A MX 2013010167 A MX2013010167 A MX 2013010167A MX 2013010167 A MX2013010167 A MX 2013010167A MX 2013010167 A MX2013010167 A MX 2013010167A MX 2013010167 A MX2013010167 A MX 2013010167A
Authority
MX
Mexico
Prior art keywords
microorganisms
engineering
thraustochytrium
thraustochytrid
methods
Prior art date
Application number
MX2013010167A
Other languages
English (en)
Other versions
MX338479B (es
Inventor
Shuocheng Zhang
Roberto Armenta
Original Assignee
Dsm Nutritional Products Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Nutritional Products Ag filed Critical Dsm Nutritional Products Ag
Publication of MX2013010167A publication Critical patent/MX2013010167A/es
Publication of MX338479B publication Critical patent/MX338479B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente descripción proporciona, inter alia, Thraustochytrium y métodos y reactivos relevantes, que incluyen secuencias reguladoras modificadas de y/o operativas en Thraustochytrid o Thraustochytrium, marcadores seleccionables útiles para modificar microrganismos tales como Thraustochytrids, medios para mutagenizar microrganismos, nuevas cepas producidas por mutagénesis, y métodos y composiciones relacionadas con la producción de compuestos particulares en microrganismos.
MX2013010167A 2011-03-07 2012-03-07 Microorganismos thraustochytrid modificados. MX338479B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161449848P 2011-03-07 2011-03-07
PCT/IB2012/000528 WO2012120375A1 (en) 2011-03-07 2012-03-07 Engineering thraustochytrid microorganisms

Publications (2)

Publication Number Publication Date
MX2013010167A true MX2013010167A (es) 2014-04-14
MX338479B MX338479B (es) 2016-04-19

Family

ID=46797543

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013010167A MX338479B (es) 2011-03-07 2012-03-07 Microorganismos thraustochytrid modificados.

Country Status (12)

Country Link
US (2) US9133463B2 (es)
EP (1) EP2683824B1 (es)
JP (1) JP6168700B2 (es)
CN (2) CN103649313B (es)
AU (1) AU2012226528B2 (es)
CA (1) CA2829296C (es)
DK (1) DK2683824T3 (es)
ES (1) ES2678997T3 (es)
MX (1) MX338479B (es)
NO (1) NO2773848T3 (es)
PT (1) PT2683824T (es)
WO (1) WO2012120375A1 (es)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2302065A1 (en) 2000-01-19 2011-03-30 Martek Biosciences Corporation Solventless extraction process
AU2011261455B2 (en) 2010-06-01 2016-03-24 Dsm Ip Assets B.V. Extraction of lipid from cells and products therefrom
CN103649313B (zh) * 2011-03-07 2017-10-24 Dsm营养产品股份公司 工程化破囊壶菌属微生物
CN105189740B (zh) * 2013-03-13 2019-01-15 帝斯曼营养品股份公司 工程化微生物
KR102435268B1 (ko) 2013-12-20 2022-08-22 디에스엠 뉴트리셔널 프라덕츠 아게 미생물로부터 오일을 회수하는 방법
US11124736B2 (en) 2013-12-20 2021-09-21 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
CN105960235B (zh) 2013-12-20 2021-01-08 帝斯曼知识产权资产管理有限公司 用于从微生物细胞获得微生物油的方法
EP2958982B1 (en) 2013-12-20 2019-10-02 Mara Renewables Corporation Methods of recovering oil from microorganisms
SG11201605052XA (en) 2013-12-20 2016-07-28 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
MX370606B (es) 2013-12-20 2019-12-18 Dsm Ip Assets Bv Procedimientos para obtener aceite microbiano a partir de células microbianas.
CN106795539B (zh) * 2014-05-22 2021-06-22 玛拉可再生能源公司 在微生物中产生油的方法
JP6773301B2 (ja) * 2014-10-16 2020-10-21 マラ リニューアブルズ コーポレーション 反復フェドバッチ培養方法
JP6806297B2 (ja) 2014-10-16 2021-01-06 マラ リニューアブルズ コーポレーション 半連続培養法
US9890402B2 (en) * 2015-01-24 2018-02-13 Indian Oil Corporation Limited Thraustochytrid based process for treating waste effluents
EP3265567B1 (en) 2015-03-02 2020-07-29 Conagen Inc. Regulatory elements from labyrinthulomycetes microorganisms
US20160264985A1 (en) * 2015-03-12 2016-09-15 Synthetic Genomics, Inc. Microorganisms for fatty acid production using elongase and desaturase enzymes
AR104042A1 (es) 2015-03-26 2017-06-21 Mara Renewables Corp Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto
EP3318640A4 (en) * 2015-07-03 2019-03-06 Kuysyu University, National University Corporation MICROORGANISM OIL-PRODUCING LABYRINTHULIDS, MICROORGANISM OIL, PREPARATION METHOD AND USES THEREOF
MX2018000502A (es) * 2015-07-13 2018-09-07 Mara Renewables Corp Mejora del metabolismo de microalga de xilosa.
FR3038914B1 (fr) * 2015-07-17 2020-03-13 Fermentalg Biomasse de thraustochytrides, procede de culture et utilisations
CN106916796B (zh) * 2015-12-28 2021-06-29 丰益(上海)生物技术研发中心有限公司 裂殖壶菌delta-12脂肪酸脱饱和酶相关序列及其应用
AU2017235543B2 (en) 2016-03-16 2020-05-14 Conagen, Inc. Production of proteins in labyrinthulomycetes
US10851395B2 (en) 2016-06-10 2020-12-01 MARA Renewables Corporation Method of making lipids with improved cold flow properties
PT3487970T (pt) 2016-07-20 2023-05-03 Mara Renewables Corp Método de produção de óleo microbiano não refinado fluido
CN109415654A (zh) 2016-07-20 2019-03-01 玛拉可再生能源公司 用于对油进行冬化的两步分馏方法
US10633454B2 (en) 2016-11-01 2020-04-28 Conagen Inc. Expression of modified glycoproteins and glycopeptides
BR112019012421A2 (pt) 2016-12-22 2020-02-27 MARA Renewables Corporation Cultura, microrganismo eucariótico, métodos para fazer uma composição lipídica e para produzir uma biomassa rica em proteínas
CN109517834B (zh) * 2018-11-27 2022-06-07 昆明藻能生物科技有限公司 一种通过遗传改造提高裂殖壶藻atcc20888油脂和dha含量的方法
WO2023144707A1 (en) 2022-01-25 2023-08-03 Dsm Ip Assets B.V. Media refinement and nutrient feeding approaches to increase polyunsaturated fatty acid production
WO2024006765A2 (en) * 2022-06-27 2024-01-04 Wisconsin Alumni Research Foundation Universal method for parasite and eukaryotic endosymbiont identification
CN117721111B (zh) * 2023-12-19 2024-06-07 海南省海洋与渔业科学院 红树植物白骨壤内源启动子amgt1p5及其应用

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US6451567B1 (en) 1988-09-07 2002-09-17 Omegatech, Inc. Fermentation process for producing long chain omega-3 fatty acids with euryhaline microorganisms
US6027900A (en) * 1996-04-12 2000-02-22 Carnegie Institution Of Washington Methods and tools for transformation of eukaryotic algae
US5968809A (en) 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US8003772B2 (en) * 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US7211418B2 (en) * 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
EP2341127B1 (en) 2000-01-28 2015-05-27 DSM IP Assets B.V. Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors
TWI324181B (en) 2001-04-16 2010-05-01 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US7211656B2 (en) 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
JP4280158B2 (ja) 2002-12-27 2009-06-17 富士フイルム株式会社 ドコサヘキサエン酸生産能を有する微生物及びその利用
US7214491B2 (en) 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
DE10352837A1 (de) * 2003-11-10 2005-07-07 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Prozess zur Kultivierung von Mikroorganismen der Gattung Thraustochytriales
DE102004063326A1 (de) 2004-12-23 2006-07-06 Basf Plant Science Gmbh Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Organismen
JP4796787B2 (ja) * 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類への遺伝子導入法
JP4796786B2 (ja) * 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類を形質転換可能なベクター
JP5886511B2 (ja) 2005-06-07 2016-03-16 ディーエスエム ニュートリショナル プロダクツ アーゲーＤＳＭ Ｎｕｔｒｉｔｉｏｎａｌ Ｐｒｏｄｕｃｔｓ ＡＧ 脂質および抗酸化剤の製造のための真核微生物
CN101573451B (zh) * 2006-03-15 2014-04-30 Dsmip资产公司 利用pufa聚酮化合物合成酶系统在异源的生物体中产生多不饱和脂肪酸的方法
WO2008129358A2 (en) 2006-08-01 2008-10-30 Ocean Nutrition Canada Ltd. Oil producing microbes and methods of modification thereof
JP2010533003A (ja) * 2007-07-13 2010-10-21 オーシャン ニュートリッション カナダ リミテッド Ｄ４デサチュラーゼ及びｄ５エロンガーゼ
TWI600762B (zh) * 2009-03-19 2017-10-01 Ｄｓｍ智慧財產有限公司 多不飽和脂肪酸合成酶核酸分子及多肽，組成物，以及其製造方法與用途
KR20130026411A (en) 2009-09-24 2013-03-13 Univ Kyushu Nat Univ Corp Method for transforming stramenopile
KR101068520B1 (ko) * 2009-10-07 2011-09-28 대상 주식회사 고함량의 도코사헥사엔산을 함유하는 오메가-3 불포화 지방산을 생산하는 균주 및 이를 이용한 오메가-3 불포화 지방산의 제조방법
AU2010343095B2 (en) 2009-12-28 2017-01-05 Sanofi Vaccine Technologies, S.A.S. Production of heterologous polypeptides in microalgae, microalgal extracellular bodies, compositions, and methods of making and uses thereof
BR112012022108B1 (pt) 2010-03-02 2022-01-18 Massachusetts Institute Technology Célula de levedura oleaginosa, cultura, bem como métodos para converter uma fonte de carboidrato em ácido graxo ou triacilglicerol e para modificar uma célula de levedura
CN103649313B (zh) * 2011-03-07 2017-10-24 Dsm营养产品股份公司 工程化破囊壶菌属微生物
CN105189740B (zh) * 2013-03-13 2019-01-15 帝斯曼营养品股份公司 工程化微生物

Also Published As

Publication number Publication date
DK2683824T3 (en) 2018-06-18
AU2012226528A1 (en) 2013-05-02
AU2012226528B2 (en) 2015-08-20
CA2829296C (en) 2019-10-29
CN107630017A (zh) 2018-01-26
CN103649313A (zh) 2014-03-19
US20160010095A1 (en) 2016-01-14
CA2829296A1 (en) 2012-09-13
ES2678997T3 (es) 2018-08-21
US9133463B2 (en) 2015-09-15
CN103649313B (zh) 2017-10-24
MX338479B (es) 2016-04-19
NO2773848T3 (es) 2018-03-31
JP2014507952A (ja) 2014-04-03
JP6168700B2 (ja) 2017-07-26
EP2683824A1 (en) 2014-01-15
PT2683824T (pt) 2018-06-07
US20120244584A1 (en) 2012-09-27
EP2683824B1 (en) 2018-04-25
US9758787B2 (en) 2017-09-12
EP2683824A4 (en) 2015-05-06
WO2012120375A1 (en) 2012-09-13

Similar Documents

Publication Publication Date Title
MX2013010167A (es) Microorganismos thraustochytrid modificados.
MY166637A (en) Method for producing ternesite
NZ711538A (en) Benzylisoquinoline alkaloids (bia) producing microbes, and methods of making and using the same
MX2023010124A (es) Generacion de acido peroxiformico a traves de formiato de alcohol polihidrico.
MX2011013700A (es) Cepa talaromyces y composiciones de enzimas.
MX2015005944A (es) Cepas microbianas, composiciones y metodos para incrementar el fosfato disponible para las plantas.
MX2018009553A (es) Metodos de seleccion.
MX2013001045A (es) Organoide hepatico, sus usos y metodo de cultivo para obtenerlos.
MX356825B (es) Metodos de control de espuma.
MX2014000984A (es) Biocontrol de nematodos.
NZ607149A (en) Galacto-oligosaccharide-containing composition and a method of producing it
UA117127C2 (uk) Спосіб одержання збагаченої вуглецем біомаси
MX371441B (es) Proyectil frangible y metodo para hacerlo.
MY165897A (en) Microorganisms having improved ornithine-producing ability and method for producing ornithine using the same
MX2013014421A (es) Procedimiento para la preparacion de una composicion que comprende complejos metalicos en una matriz organica aceitosa.
IN2014DN10078A (es)
WO2012169819A3 (ko) 2-하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트의 제조방법
EA201490275A1 (ru) Композиции и способы получения изопрена
BG111535A (bg) Метод за катализиране изгарянето на въглеродсъдържащи твърди горива
PH12017500971A1 (en) Synergistic bacterial consortia for mobilizing soil phosphorus
IN2014MN01671A (es)
GB2452892A (en) Process for culturing bacteria of the piscirickettsia genus
MX352071B (es) Ésteres de lactato eterificados, método de producción de los mismos y uso de los mismos para intensificar el efecto de agentes protectores de plantas.
MX339066B (es) Composicion limpiadora para tela que comprende agente tonalizador.
TN2014000329A1 (en) Method for preparing compound by novel michael addition reaction using water or various acids as additive

Legal Events

Date Code Title Description
FG Grant or registration