BR112017000846A2 - bactérias termofílicas geneticamente modificadas que produzem ácido (r)-láctico - Google Patents

bactérias termofílicas geneticamente modificadas que produzem ácido (r)-láctico

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Publication number
BR112017000846A2
BR112017000846A2 BR112017000846-7A BR112017000846A BR112017000846A2 BR 112017000846 A2 BR112017000846 A2 BR 112017000846A2 BR 112017000846 A BR112017000846 A BR 112017000846A BR 112017000846 A2 BR112017000846 A2 BR 112017000846A2
Authority
BR
Brazil
Prior art keywords
lactic acid
genetically modified
thermophilic bacteria
bacteria producing
modified thermophilic
Prior art date
Application number
BR112017000846-7A
Other languages
English (en)
Other versions
BR112017000846B1 (pt
Inventor
Petrus Machielsen Marinus
Van Hartskamp Mariska
Van Kranenburg Richard
Original Assignee
Purac Biochem B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purac Biochem B.V. filed Critical Purac Biochem B.V.
Publication of BR112017000846A2 publication Critical patent/BR112017000846A2/pt
Publication of BR112017000846B1 publication Critical patent/BR112017000846B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01028—D-Lactate dehydrogenase (1.1.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01054—Formate C-acetyltransferase (2.3.1.54), i.e. pyruvate formate-lyase or PFL
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03003—Methylglyoxal synthase (4.2.3.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01008—Phosphate acetyltransferase (2.3.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

a presente invenção refere-se a uma célula bacteriana termofílica modificada por engenharia genética que é anaeróbica facultativa compreendendo a) inativação ou deleção do gene endógeno de (s)-lactato desidrogenase; b) introdução de um gene de (r)-lactato desidrogenase; c) inativação ou deleção do gene endógeno de piruvato formato liase a e / ou b.
BR112017000846-7A 2014-07-23 2015-07-13 Bactérias termofílicas do gênero geobacillus geneticamente modificadas que produzem ácido (r)-láctico e método para produzir ácido (r)- láctico enantiomérico puro BR112017000846B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14178144.3 2014-07-23
EP14178144 2014-07-23
PCT/EP2015/065990 WO2016012296A1 (en) 2014-07-23 2015-07-13 Genetically modified (r)-lactic acid producing thermophilic bacteria

Publications (2)

Publication Number Publication Date
BR112017000846A2 true BR112017000846A2 (pt) 2018-07-31
BR112017000846B1 BR112017000846B1 (pt) 2024-01-16

Family

ID=51211697

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017000846-7A BR112017000846B1 (pt) 2014-07-23 2015-07-13 Bactérias termofílicas do gênero geobacillus geneticamente modificadas que produzem ácido (r)-láctico e método para produzir ácido (r)- láctico enantiomérico puro

Country Status (12)

Country Link
US (1) US10731186B2 (pt)
EP (1) EP3194592B1 (pt)
JP (1) JP6637026B2 (pt)
KR (1) KR101980069B1 (pt)
CN (1) CN106574236B (pt)
AU (1) AU2015294136C9 (pt)
BR (1) BR112017000846B1 (pt)
CA (1) CA2955717C (pt)
ES (1) ES2821418T3 (pt)
PL (1) PL3194592T3 (pt)
PT (1) PT3194592T (pt)
WO (1) WO2016012296A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018109101A1 (en) 2016-12-14 2018-06-21 Wageningen Universiteit Thermostable cas9 nucleases
CA3119086A1 (en) 2018-11-30 2020-06-04 Utilization Of Carbon Dioxide Institute Co., Ltd. Lactic acid-producing hydrogenophilus bacterium transformant
JP6675574B1 (ja) 2019-08-09 2020-04-01 株式会社Ｃｏ２資源化研究所 乳酸を生成する遺伝子組換えヒドロゲノフィラス属細菌
CN111154705B (zh) * 2020-01-07 2021-06-29 中国科学院微生物研究所 热葡萄糖苷酶地芽孢杆菌工程菌及其构建方法及应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4336082B2 (ja) 2001-05-29 2009-09-30 花王株式会社 宿主微生物
GB0117551D0 (en) * 2001-07-18 2001-09-12 Elsworth Biotech Ltd Lastic acid production
US9497168B2 (en) 2002-07-30 2016-11-15 Avaya Inc. Method and apparatus for supporting communications between a computing device within a network and an external computing device
CN1809631B (zh) 2003-06-18 2010-05-26 帝斯曼知识产权资产管理有限公司 用不能形成芽孢的微生物来生产泛酸盐/酯的方法
US7098009B2 (en) * 2004-03-04 2006-08-29 University Of Florida Research Foundation, Inc. Production of chemicals from lignocellulose, biomass or sugars
PL2305826T3 (pl) * 2005-08-10 2016-10-31 Materiały i sposoby do wydajnej produkcji kwasu mlekowego
WO2007120198A2 (en) 2005-11-08 2007-10-25 University Of Florida Research Foundation, Inc. Materials and methods for improved microbial production of organic compounds
EP1989309B1 (en) * 2006-01-24 2011-08-31 PURAC Biochem BV Genetic modification of homolactic thermophilic bacilli
CA2818680A1 (en) * 2010-11-22 2012-05-31 Qingzhao Wang Engineering of thermotolerant bacillus coagulans for production of d(-)-lactic acid
KR101438882B1 (ko) 2012-04-24 2014-09-05 씨제이제일제당 (주) 신규한 ｄ형 젖산 생산균주
JPWO2014017469A1 (ja) * 2012-07-23 2016-07-11 三井化学株式会社 Ｄ−乳酸の生産方法およびポリマーの生産方法

Also Published As

Publication number Publication date
CA2955717A1 (en) 2016-01-28
JP2017523778A (ja) 2017-08-24
EP3194592A1 (en) 2017-07-26
EP3194592B1 (en) 2020-07-08
AU2015294136C1 (en) 2018-10-25
KR101980069B1 (ko) 2019-05-20
PL3194592T3 (pl) 2021-02-08
AU2015294136C9 (en) 2019-05-02
AU2015294136A1 (en) 2017-02-09
BR112017000846B1 (pt) 2024-01-16
WO2016012296A1 (en) 2016-01-28
US10731186B2 (en) 2020-08-04
US20170275656A1 (en) 2017-09-28
CA2955717C (en) 2020-09-22
AU2015294136B2 (en) 2018-04-26
CN106574236B (zh) 2020-12-11
CN106574236A (zh) 2017-04-19
KR20170019467A (ko) 2017-02-21
ES2821418T3 (es) 2021-04-26
PT3194592T (pt) 2020-09-25
JP6637026B2 (ja) 2020-01-29

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Legal Events

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 13/07/2015, OBSERVADAS AS CONDICOES LEGAIS