BR102013031330A8 - processo de cultivo de células-tronco mesenquimais adultas, kit, composição e usos - Google Patents

processo de cultivo de células-tronco mesenquimais adultas, kit, composição e usos

Info

Publication number
BR102013031330A8
BR102013031330A8 BR102013031330A BR102013031330A BR102013031330A8 BR 102013031330 A8 BR102013031330 A8 BR 102013031330A8 BR 102013031330 A BR102013031330 A BR 102013031330A BR 102013031330 A BR102013031330 A BR 102013031330A BR 102013031330 A8 BR102013031330 A8 BR 102013031330A8
Authority
BR
Brazil
Prior art keywords
stem cells
mesenchymal stem
adult mesenchymal
composition
kit
Prior art date
Application number
BR102013031330A
Other languages
English (en)
Other versions
BR102013031330A2 (pt
BR102013031330B1 (pt
Inventor
Falavigna Asdrubal
Cantarelli Machado Denise
Silveira De Aguiar Israel
Antonio Pêgas Henriques João
Figueiró Manuela
Roesch Ely Mariana
Original Assignee
Fundacao Univ De Caxias Do Sul
Ubea
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fundacao Univ De Caxias Do Sul, Ubea filed Critical Fundacao Univ De Caxias Do Sul
Priority to BR102013031330-0A priority Critical patent/BR102013031330B1/pt
Priority to PCT/BR2014/050033 priority patent/WO2015081410A1/pt
Publication of BR102013031330A2 publication Critical patent/BR102013031330A2/pt
Publication of BR102013031330A8 publication Critical patent/BR102013031330A8/pt
Publication of BR102013031330B1 publication Critical patent/BR102013031330B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)

Abstract

processo de cultivo de células-tronco mesenquimais adultas, kit, composição e usos. a presente invenção descreve um novo processo de cultivo de células-tronco mesenquimais adultas, sendo que o dito processo compreende as etapas de: a) incubação de tecido ex-vivo degenerado em meio de cultura; b) isolamento das células-tronco mesenquimais adultas do meio obtido na etapa a); e c) cultura das células-tronco mesenquimais adultas isoladas na etapa b). ainda, o método em questão dispensa etapas de dissociação mecânica e enzimática usualmente empregadas na técnica. a presente invenção provê também um kit para o cultivo de células-tronco mesenquimais adultas, que compreende: a) recipiente de material antiaderente; b) meio de cultura adequado; e c) recipiente adequado ao cultivo celular. a presente invenção descreve adicionalmente uma composição que compreende as células-tronco mesenquimais adultas obtidas por processo conforme definido anteriormente, e veículo farmaceuticamente aceitável. ainda, a presente invenção descreve o uso das células-tronco mesenquimais adultas obtidas por processo conforme definido anteriormente para a fabricação de uma composição para o tratamento de discopatias do disco intervertebral degenerado.
BR102013031330-0A 2013-12-05 2013-12-05 Processo de cultivo de células-tronco mesenquimais adultas BR102013031330B1 (pt)

Priority Applications (2)

Application Number Priority Date Filing Date Title
BR102013031330-0A BR102013031330B1 (pt) 2013-12-05 2013-12-05 Processo de cultivo de células-tronco mesenquimais adultas
PCT/BR2014/050033 WO2015081410A1 (pt) 2013-12-05 2014-12-05 Processo de cultivo de células-tronco mesenquimais adultas, kit, composição e usos

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BR102013031330-0A BR102013031330B1 (pt) 2013-12-05 2013-12-05 Processo de cultivo de células-tronco mesenquimais adultas

Publications (3)

Publication Number Publication Date
BR102013031330A2 BR102013031330A2 (pt) 2015-09-29
BR102013031330A8 true BR102013031330A8 (pt) 2021-02-23
BR102013031330B1 BR102013031330B1 (pt) 2022-03-03

Family

ID=53272668

Family Applications (1)

Application Number Title Priority Date Filing Date
BR102013031330-0A BR102013031330B1 (pt) 2013-12-05 2013-12-05 Processo de cultivo de células-tronco mesenquimais adultas

Country Status (2)

Country Link
BR (1) BR102013031330B1 (pt)
WO (1) WO2015081410A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111317747A (zh) * 2020-03-24 2020-06-23 北京大学口腔医学院 肠道菌群调节剂与间充质干细胞的组合物及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6835377B2 (en) * 1998-05-13 2004-12-28 Osiris Therapeutics, Inc. Osteoarthritis cartilage regeneration
WO2012070001A1 (en) * 2010-11-24 2012-05-31 Stempeutics Research Pvt. Ltd. An explant culture technique for isolation of mesenchymal stem cells from adipose tissue
WO2013148334A1 (en) * 2012-03-27 2013-10-03 Parcell Laboratories, Llc Intervertebral disc repair compositions and methods

Also Published As

Publication number Publication date
BR102013031330A2 (pt) 2015-09-29
WO2015081410A1 (pt) 2015-06-11
BR102013031330B1 (pt) 2022-03-03

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Legal Events

Date Code Title Description
B03A Publication of a patent application or of a certificate of addition of invention [chapter 3.1 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B03H Publication of an application: rectification [chapter 3.8 patent gazette]

Free format text: REFERENTE A RPI 2334 DE 29/09/2015, QUANTO AO ITEM (71).

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 05/12/2013, OBSERVADAS AS CONDICOES LEGAIS.