WO2014053418A3 - Method for obtaining mesenchymal stem cells and use thereof - Google Patents

Method for obtaining mesenchymal stem cells and use thereof Download PDF

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Publication number
WO2014053418A3
WO2014053418A3 PCT/EP2013/070247 EP2013070247W WO2014053418A3 WO 2014053418 A3 WO2014053418 A3 WO 2014053418A3 EP 2013070247 W EP2013070247 W EP 2013070247W WO 2014053418 A3 WO2014053418 A3 WO 2014053418A3
Authority
WO
WIPO (PCT)
Prior art keywords
mscs
stem cells
mesenchymal stem
obtaining mesenchymal
body fluid
Prior art date
Application number
PCT/EP2013/070247
Other languages
French (fr)
Other versions
WO2014053418A2 (en
WO2014053418A9 (en
Inventor
Sarah Yolande Kristel BROECKX
Jan Hilda Marie Jozef SPAAS
Original Assignee
Global Stem Cell Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Global Stem Cell Technology filed Critical Global Stem Cell Technology
Publication of WO2014053418A2 publication Critical patent/WO2014053418A2/en
Publication of WO2014053418A3 publication Critical patent/WO2014053418A3/en
Publication of WO2014053418A9 publication Critical patent/WO2014053418A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders

Abstract

The present invention relates to a method for obtaining mesenchymal stem cells (MSCs) from mammalian tissue or body fluid, comprising isolating said MSCs from tissue or body fluid and expanding said isolated MSCs in multiple cultivation passages, characterized in that the cell doubling log (CDL) per 24 hours of the MSCs is between 0.25 and 3.2 during expansion. In a further aspect, the current invention also relates to cell compositions obtained by the current method and to a container for storing such compositions.
PCT/EP2013/070247 2012-10-01 2013-09-27 Method for obtaining mesenchymal stem cells and use thereof WO2014053418A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BE2012/0656A BE1020480A5 (en) 2012-10-01 2012-10-01 METHOD FOR INSULATION OF MESENCHYMAL STEM CELLS FROM BIRD BLOOD AND USE THEREOF
BEBE2012/0656 2012-10-01

Publications (3)

Publication Number Publication Date
WO2014053418A2 WO2014053418A2 (en) 2014-04-10
WO2014053418A3 true WO2014053418A3 (en) 2014-05-30
WO2014053418A9 WO2014053418A9 (en) 2014-07-17

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/EP2013/070247 WO2014053418A2 (en) 2012-10-01 2013-09-27 Method for obtaining mesenchymal stem cells and use thereof
PCT/EP2013/070257 WO2014053420A1 (en) 2012-10-01 2013-09-27 Method for the isolation of mesenchymal stem cells from mammalian blood and use thereof

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/EP2013/070257 WO2014053420A1 (en) 2012-10-01 2013-09-27 Method for the isolation of mesenchymal stem cells from mammalian blood and use thereof

Country Status (2)

Country Link
BE (1) BE1020480A5 (en)
WO (2) WO2014053418A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3077503A1 (en) * 2013-12-06 2016-10-12 Global Stem Cell Technology Method and composition for inducing chondrogenesis or tenogenesis in mesenchymal stem cells
EP3666298A1 (en) 2018-12-13 2020-06-17 Global Stem Cell Technology A collagen formulation suitable for injection
TR201911506A2 (en) * 2019-07-30 2021-02-22 T C Erciyes Ueniversitesi FIBRIN-WELDED MESENCHIMAL STEM CELLS RICH THROMBOSITE
US20220354894A1 (en) 2019-09-27 2022-11-10 Boehringer Ingelheim Veterinary Medicine Belgium Cell Composition Comprising Radiolabled Mesenchymal Stem Cells, Use Thereof and Method for Preparing Radiolabeled Mesenchymal Stem Cells
CN111733128A (en) * 2020-05-14 2020-10-02 厚朴生物科技（苏州）有限公司 Preparation method of human adipose-derived mesenchymal stem cells and in-vitro differentiation capacity identification method
CN112116555B (en) * 2020-08-12 2024-03-26 清华大学深圳国际研究生院 Method for detecting physiological functions of mesenchymal stem cells and application thereof
CN112402364B (en) * 2020-10-23 2023-08-04 中科细胞科技(广州)有限公司 Umbilical cord mesenchymal stem cell-platelet-rich plasma-containing composite repair gel for injection
WO2022155213A1 (en) * 2021-01-12 2022-07-21 Qcdx Llc Detection and analysis of circulating tumor cells
TW202319059A (en) * 2021-07-08 2023-05-16 比利時商比利時百靈佳殷格翰動物醫藥公司 Mesenchymal stem cells for use in the treatment of osteoarthritis in animals
US20230012590A1 (en) 2021-07-08 2023-01-19 Boehringer Ingelheim Veterinary Medicine Belgium Mesenchymal stem cells for use in the treatment of chronic gingivostomatitis
TW202319536A (en) 2021-07-08 2023-05-16 比利時商比利時百靈佳殷格翰動物醫藥公司 Mesenchymal stem cells for use in the treatment of chronic kidney disease
US20230014698A1 (en) 2021-07-08 2023-01-19 Boehringer Ingelheim Veterinary Medicine Belgium Mesenchymal stem cells for use in the treatment of atopic dermatitis
WO2024033462A1 (en) 2022-08-11 2024-02-15 Boehringer Ingelheim Vetmedica Gmbh Mesenchymal stem cells for use in the treatment of insect-bite hypersensitivity in equines

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITRM20060498A1 (en) * 2006-09-20 2008-03-21 Alessandra Gambacurta METHOD OF EXPANSION OF ADULT STEM CELLS FROM PERIPHERAL BLOOD AND RELATED USES IN MEDICAL FIELD
ES2326772B1 (en) * 2007-09-26 2010-07-26 Fundacion Progreso Y Salud PROCESS FOR OBTAINING MESENQUIMAL MOTHER CELLS WITH PLURIPOTENT CAPACITY.
WO2011069121A1 (en) * 2009-12-04 2011-06-09 Neostem, Inc. Mesenchymal stem cells (mscs) isolated from mobilized peripheral blood
ES2384790B1 (en) * 2010-12-10 2013-05-20 Instituto De Salud Carlos Iii MESENQUIMAL MOTHER CELLS ISOLATED FROM PERIPHERAL BLOOD.

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
HOYNOWSKI S M ET AL: "Characterization and differentiation of equine umbilical cord-derived matrix cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 362, no. 2, 31 August 2007 (2007-08-31), pages 347 - 353, XP022226298, ISSN: 0006-291X, DOI: 10.1016/J.BBRC.2007.07.182 *
HOYNOWSKI S M ET AL: "Corrigendum to "Characterization and differentiation of equine umbilical cord-derived matrix cells"", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 373, no. 1, 15 August 2008 (2008-08-15), pages 177, XP055097388, ISSN: 0006-291X, DOI: 10.1016/j.bbrc.2007.07.182 *
IACONO E ET AL: "Isolation, characterization and differentiation of mesenchymal stem cells from amniotic fluid, umbilical cord blood and Wharton's jelly in the horse", REPRODUCTION, vol. 143, no. 4, 24 January 2012 (2012-01-24), pages 455 - 468, XP055094994, ISSN: 1470-1626, DOI: 10.1530/REP-10-0408 *
MARTINELLO T ET AL: "Cryopreservation Does Not Affect the Stem Characteristics of Multipotent Cells Isolated from Equine Peripheral Blood", TISSUE ENGINEERING PART C: METHODS, vol. 16, no. 4, August 2010 (2010-08-01), pages 771 - 781, XP055069017, ISSN: 1937-3384, DOI: 10.1089/ten.tec.2009.0512 *
SPAAS J H ET AL: "Culture and characterisation of equine peripheral blood mesenchymal stromal cells", THE VETERINARY JOURNAL, vol. 195, no. 1, January 2013 (2013-01-01), pages 107 - 113, XP055049712, ISSN: 1090-0233, [retrieved on 20120618], DOI: 10.1016/j.tvjl.2012.05.006 *
TAGHI G M ET AL: "Characterization of in vitro cultured bone marrow and adipose tissue-derived mesenchymal stem cells and their ability to express neurotrophic factors", CELL BIOLOGY INTERNATIONAL, vol. 36, no. 12, December 2012 (2012-12-01), pages 1239 - 1249, XP055096356, ISSN: 1065-6995, DOI: 10.1042/CBI20110618 *
VIDAL M A ET AL: "Cell Growth Characteristics and Differentiation Frequency of Adherent Equine Bone Marrow?Derived Mesenchymal Stromal Cells: Adipogenic and Osteogenic Capacity", VETERINARY SURGERY, vol. 35, no. 7, October 2006 (2006-10-01), pages 601 - 610, XP055096350, ISSN: 0161-3499, DOI: 10.1111/j.1532-950X.2006.00197.x *
ZHU X ET AL: "The comparition of biological characteristics and multilineage differentiation of bone marrow and adipose derived Mesenchymal stem cells", CELL AND TISSUE RESEARCH, vol. 350, no. 2, 5 June 2012 (2012-06-05), pages 277 - 287, XP035129474, ISSN: 1432-0878, DOI: 10.1007/S00441-012-1453-1 *

Also Published As

Publication number Publication date
WO2014053418A2 (en) 2014-04-10
WO2014053418A9 (en) 2014-07-17
WO2014053420A1 (en) 2014-04-10
BE1020480A5 (en) 2013-11-05

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