BR112013020773A2 - "método para a detecção localizada in situ de rna, método para a determinação da presença e da localização de uma sequência genética, conjunto de sondas em cadeado, uso do conjunto de sondas, kit e método para a identificação de uma célula" - Google Patents

"método para a detecção localizada in situ de rna, método para a determinação da presença e da localização de uma sequência genética, conjunto de sondas em cadeado, uso do conjunto de sondas, kit e método para a identificação de uma célula"

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BR112013020773A2
BR112013020773A2 BR112013020773A BR112013020773A BR112013020773A2 BR 112013020773 A2 BR112013020773 A2 BR 112013020773A2 BR 112013020773 A BR112013020773 A BR 112013020773A BR 112013020773 A BR112013020773 A BR 112013020773A BR 112013020773 A2 BR112013020773 A2 BR 112013020773A2
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rna
probe pool
situ
padlock
kit
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BR112013020773A
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BR112013020773B1 (pt
BR112013020773A8 (pt
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Chatarina Larsson
Ida Grundberg
Mats Nilsson Bernitz
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Leica Biosistems Newcastle Ltd
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Publication of BR112013020773B1 publication Critical patent/BR112013020773B1/pt

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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Abstract

resumo patente de invenção: "método para a detecção localizada in situ de mrna". a presente invenção refere-se à detecção de rna, em uma amostra de células. mais particularmente, a presente invenção refere-se à detecção localizada de rna in situ. o método baseia - se na conversão do rna para dna complementar antes da segmentação do cdna com a (s) sonda (s) em cadeado. a hibridização da (s) sonda (s) em cadeado depende da sequência de nucleotídeos do cdna que é derivada a partir da sequência de nucleotídeos correspondente do rna alvo. a amplificação de círculo rolante da sonda em cadeado subsequentemente circularizada produz um produto de círculo rolante que pode ser detectado. vantajosamente, isto permite que o rna venha a ser detectado in situ.
BR112013020773-6A 2011-02-15 2012-02-15 Método para a detecção localizada in situ de rna, método para a determinação da presença e da localização de uma sequência genética e método para a localização e/ou detecção in situ de um rna específico BR112013020773B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161442921P 2011-02-15 2011-02-15
US61/442,921 2011-02-15
US201161473662P 2011-04-08 2011-04-08
US61/473,662 2011-04-08
PCT/IB2012/000995 WO2012110899A2 (en) 2011-02-15 2012-02-15 Method for localizedin situdetection of mrna

Publications (3)

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BR112013020773A2 true BR112013020773A2 (pt) 2016-11-29
BR112013020773A8 BR112013020773A8 (pt) 2018-05-02
BR112013020773B1 BR112013020773B1 (pt) 2023-01-24

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BR112013020773-6A BR112013020773B1 (pt) 2011-02-15 2012-02-15 Método para a detecção localizada in situ de rna, método para a determinação da presença e da localização de uma sequência genética e método para a localização e/ou detecção in situ de um rna específico

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US (2) US8551708B2 (pt)
EP (1) EP2675916B1 (pt)
JP (2) JP5881746B2 (pt)
CN (2) CN103649331A (pt)
AU (1) AU2012219132B2 (pt)
BR (1) BR112013020773B1 (pt)
CA (1) CA2827497C (pt)
WO (1) WO2012110899A2 (pt)

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