BR112013033443A2 - método para avaliar a atividade redox da molécula de ácido nucleico e da molécula de ácido nucleico com atividade redox - Google Patents

método para avaliar a atividade redox da molécula de ácido nucleico e da molécula de ácido nucleico com atividade redox

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Publication number
BR112013033443A2
BR112013033443A2 BR112013033443A BR112013033443A BR112013033443A2 BR 112013033443 A2 BR112013033443 A2 BR 112013033443A2 BR 112013033443 A BR112013033443 A BR 112013033443A BR 112013033443 A BR112013033443 A BR 112013033443A BR 112013033443 A2 BR112013033443 A2 BR 112013033443A2
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Brazil
Prior art keywords
nucleic acid
acid molecule
redox activity
redox
evaluated
Prior art date
Application number
BR112013033443A
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English (en)
Inventor
Waga Iwao
Akitomi Jou
Horii Katsunori
Kaneko Naoto
Katou Shintarou
Original Assignee
Nec Software Ltd
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Publication date
Application filed by Nec Software Ltd filed Critical Nec Software Ltd
Publication of BR112013033443A2 publication Critical patent/BR112013033443A2/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/09—Recombinant DNA-technology
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G—PHYSICS
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays

Abstract

resumo patente de invenção: "método para avaliar a atividade redox da molécula de ácido nucleico e da molécula de ácido nucleico com atividade redox". a presente invenção fornece uma nova técnica pela qual a ativi-dade redox de uma molécula de ácido nucleico pode ser avaliada. um méto-do de avaliação da presente invenção inclui: uma etapa de detecção para detectar eletroquimicamente uma reação redox a um substrato, a reação redox é catalisada por uma molécula de ácido nucleico a ser avaliada, utili-zando um dispositivo que detecta eletroquimicamente uma reação redox; e uma etapa de avaliação para avaliar a atividade redox de uma molécula de ácido nucleico a partir de um resultado da detecção da reação redox. como o dispositivo, um dispositivo no qual uma base fornecida com uma parte de detecção é incluída, a parte de detecção inclui um sistema de eletrodo, e a molécula de ácido nucleico a ser avaliada é disposta na base e é utilizada. na presente invenção, é preferencial que uma pluralidade de tipos de molécula de ácido nucleico a ser avaliada é disposta na base, e a pluralidade de tipos de moléculas de ácido nucleico a ser avaliada é avaliada por um único dispositivo.
BR112013033443A 2011-07-04 2012-07-02 método para avaliar a atividade redox da molécula de ácido nucleico e da molécula de ácido nucleico com atividade redox BR112013033443A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2011148562 2011-07-04
PCT/JP2012/066912 WO2013005723A1 (ja) 2011-07-04 2012-07-02 核酸分子の酸化還元活性を評価する方法および酸化還元活性を有する核酸分子

Publications (1)

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BR112013033443A2 true BR112013033443A2 (pt) 2017-01-31

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BR112013033443A BR112013033443A2 (pt) 2011-07-04 2012-07-02 método para avaliar a atividade redox da molécula de ácido nucleico e da molécula de ácido nucleico com atividade redox

Country Status (7)

Country Link
US (2) US9637737B2 (pt)
EP (1) EP2730915A4 (pt)
JP (2) JP5863794B2 (pt)
CN (2) CN104388424B (pt)
BR (1) BR112013033443A2 (pt)
HK (1) HK1204653A1 (pt)
WO (1) WO2013005723A1 (pt)

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EP2886664A4 (en) * 2012-07-27 2016-04-06 Nec Solution Innovators Ltd MELAMINE ANALYSIS NUCLEIC ACID DETECTOR, ANALYSIS DEVICE AND ANALYSIS METHOD
JP6041408B2 (ja) * 2013-03-08 2016-12-07 Ｎｅｃソリューションイノベータ株式会社 核酸素子候補分子、および、これを用いたターゲット分析用核酸素子のスクリーニング方法
WO2015001856A1 (ja) * 2013-07-01 2015-01-08 Ｎｅｃソリューションイノベータ株式会社 属性推定システム
JP6218250B2 (ja) * 2013-07-23 2017-10-25 Ｎｅｃソリューションイノベータ株式会社 ターゲット分析用センサ、ターゲット分析用デバイス、および、これを用いたターゲットの分析方法
JP6347498B2 (ja) * 2014-03-31 2018-06-27 Ｎｅｃソリューションイノベータ株式会社 卵アレルゲンに結合する核酸分子およびその用途
JP7012299B2 (ja) * 2016-04-28 2022-01-28 国立大学法人東京農工大学 ペルオキシダーゼ活性増大アプタマー
WO2017188426A1 (ja) * 2016-04-28 2017-11-02 国立大学法人東京農工大学 ペルオキシダーゼ活性増大アプタマー

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Also Published As

Publication number Publication date
US20140128589A1 (en) 2014-05-08
CN103649741B (zh) 2016-11-16
EP2730915A4 (en) 2015-06-17
EP2730915A1 (en) 2014-05-14
JP5863794B2 (ja) 2016-02-17
JPWO2013005723A1 (ja) 2015-02-23
US10138480B2 (en) 2018-11-27
CN104388424A (zh) 2015-03-04
US20160060630A1 (en) 2016-03-03
WO2013005723A1 (ja) 2013-01-10
US9637737B2 (en) 2017-05-02
EP2730915A9 (en) 2015-03-25
CN104388424B (zh) 2017-07-07
HK1204653A1 (en) 2015-11-27
CN103649741A (zh) 2014-03-19
JP2016028255A (ja) 2016-02-25
JP6281953B2 (ja) 2018-02-21

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]
B25E Requested change of name of applicant rejected

Owner name: NEC SOFT, LTD. (JP)