EA201590455A1 - Способ анализа нуклеиновой кислоты-мишени, набор и анализатор - Google Patents

Способ анализа нуклеиновой кислоты-мишени, набор и анализатор

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Publication number
EA201590455A1
EA201590455A1 EA201590455A EA201590455A EA201590455A1 EA 201590455 A1 EA201590455 A1 EA 201590455A1 EA 201590455 A EA201590455 A EA 201590455A EA 201590455 A EA201590455 A EA 201590455A EA 201590455 A1 EA201590455 A1 EA 201590455A1
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EA
Eurasian Patent Office
Prior art keywords
nucleic acid
target nucleic
primer
probe
analysis
Prior art date
Application number
EA201590455A
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English (en)
Other versions
EA032482B1 (ru
Inventor
Йосихиде Хайясизаки
Масаеси Итох
Такахиро Аракава
Кенго Усуи
Сотаро Уемура
Ясумаса Митани
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КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ
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Publication of EA201590455A1 publication Critical patent/EA201590455A1/ru
Publication of EA032482B1 publication Critical patent/EA032482B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/04—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups one >CH- group, e.g. cyanines, isocyanines, pseudocyanines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/06—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups three >CH- groups, e.g. carbocyanines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Изобретение связано со способом анализа нуклеиновой кислоты-мишени, посредством которого нуклеиновую кислоту-мишень можно быстро и легко анализировать. Для достижения указанной цели в изобретении предложен способ анализа нуклеиновой кислоты-мишени в образце, включающий в себя следующие стадии: анализ нуклеиновой кислоты-мишени в образце путем приведения образца в контакт с меткой и с праймером или зондом, который может гибридизоваться с нуклеиновой кислотой-мишенью. Праймер или зонд иммобилизован на твердой фазе. Метка не излучает свет, когда праймер или зонд не гибридизуются с нуклеиновой кислотой-мишенью, тогда как, если праймер или зонд гибридизуются с нуклеиновой кислотой-мишенью, излучение света меткой происходит. Анализ осуществляют путем детекции излучаемого меткой света.
EA201590455A 2012-08-30 2013-08-29 Способ анализа нуклеиновой кислоты-мишени EA032482B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2012190715 2012-08-30
PCT/JP2013/073233 WO2014034818A1 (ja) 2012-08-30 2013-08-29 標的核酸の分析方法、キットおよび分析機器

Publications (2)

Publication Number Publication Date
EA201590455A1 true EA201590455A1 (ru) 2015-11-30
EA032482B1 EA032482B1 (ru) 2019-06-28

Family

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Family Applications (1)

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EA201590455A EA032482B1 (ru) 2012-08-30 2013-08-29 Способ анализа нуклеиновой кислоты-мишени

Country Status (5)

Country Link
US (1) US10066264B2 (ru)
EP (1) EP2891714B1 (ru)
JP (1) JP5598784B2 (ru)
EA (1) EA032482B1 (ru)
WO (1) WO2014034818A1 (ru)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA201692118A1 (ru) * 2012-07-16 2017-05-31 КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ Зонд на основе нуклеиновой кислоты, способ конструирования зонда на основе нуклеиновой кислоты и способ обнаружения последовательности-мишени
WO2017038682A1 (ja) * 2015-08-28 2017-03-09 国立研究開発法人理化学研究所 鋳型核酸の分析方法、標的物質の分析方法、鋳型核酸または標的物質の分析用キット、および鋳型核酸または標的物質の分析用装置
WO2018008435A1 (ja) * 2016-07-04 2018-01-11 株式会社ダナフォーム 核酸分析方法
WO2018073872A1 (ja) 2016-10-17 2018-04-26 オリンパス株式会社 標的核酸分子の検出方法
US20180188204A1 (en) * 2017-01-01 2018-07-05 Sylvester Tumusiime Use of charged quinine sulfate or other precursors or derivatives of quinine alkaloids in visualization of nucleic acids
KR102103719B1 (ko) * 2018-05-18 2020-04-23 주식회사 바이나리 등온핵산 증폭을 이용한 생체조직의 3차원 핵산영상 분석 방법
EP4114970A4 (en) * 2020-03-02 2024-04-03 Northwestern University ADJUSTMENT FUSES FOR DETECTION OF INTRACELLULAR ANALYTES IN LIVING CELLS
GB2605404A (en) * 2021-03-30 2022-10-05 Sumitomo Chemical Co Sequencing method
CN115260261A (zh) * 2021-04-30 2022-11-01 苏州诺维康生物科技有限公司 一种荧光染料修饰的脱氧核苷固相载体的制备方法

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JPH0723800A (ja) 1993-07-08 1995-01-27 Tanabe Seiyaku Co Ltd 核酸の検出方法
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
IT1303767B1 (it) 1998-11-17 2001-02-23 San Raffaele Centro Fond Metodo di quantificazione di acidi nucleici.
AUPR050700A0 (en) 2000-10-03 2000-10-26 Id+Plus Ltd Detection method
CA2622649C (en) 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
JP4370385B2 (ja) * 2007-03-09 2009-11-25 独立行政法人理化学研究所 プライマー、プライマーセット、それを用いた核酸増幅方法および変異検出方法
JP4761086B2 (ja) 2007-03-09 2011-08-31 独立行政法人理化学研究所 核酸、標識物質、核酸検出方法およびキット
EP2116614A1 (en) 2008-05-06 2009-11-11 Qiagen GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
WO2012091091A1 (ja) 2010-12-28 2012-07-05 独立行政法人理化学研究所 化合物、核酸、核酸の製造方法および核酸を製造するためのキット
EA201692118A1 (ru) * 2012-07-16 2017-05-31 КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ Зонд на основе нуклеиновой кислоты, способ конструирования зонда на основе нуклеиновой кислоты и способ обнаружения последовательности-мишени

Also Published As

Publication number Publication date
US20150152496A1 (en) 2015-06-04
JP5598784B2 (ja) 2014-10-01
US10066264B2 (en) 2018-09-04
EP2891714A4 (en) 2016-05-11
EA032482B1 (ru) 2019-06-28
EP2891714B1 (en) 2018-07-11
EP2891714A1 (en) 2015-07-08
WO2014034818A1 (ja) 2014-03-06
JPWO2014034818A1 (ja) 2016-08-08

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