AU572002B2 - Regulatory dna fragment from pichia for heterologous gene expression in yeast - Google Patents

Regulatory dna fragment from pichia for heterologous gene expression in yeast

Info

Publication number
AU572002B2
AU572002B2 AU48752/85A AU4875285A AU572002B2 AU 572002 B2 AU572002 B2 AU 572002B2 AU 48752/85 A AU48752/85 A AU 48752/85A AU 4875285 A AU4875285 A AU 4875285A AU 572002 B2 AU572002 B2 AU 572002B2
Authority
AU
Australia
Prior art keywords
pichia
yeast
gene expression
dna fragment
heterologous gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
AU48752/85A
Other languages
English (en)
Other versions
AU4875285A (en
Inventor
Paul Frederick Brust
Steven Bradley Ellis
Thomas Raymond Gingeras
Michael Miller Harpold
David Womack Stroman
Juerg Friedrich Tschopp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Research Corp Technologies Inc
Original Assignee
Phillips Petroleum Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27099454&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU572002(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US06/666,391 external-priority patent/US4808537A/en
Application filed by Phillips Petroleum Co filed Critical Phillips Petroleum Co
Publication of AU4875285A publication Critical patent/AU4875285A/en
Application granted granted Critical
Publication of AU572002B2 publication Critical patent/AU572002B2/en
Assigned to RESEARCH CORPORATION TECHNOLOGIES, INC. reassignment RESEARCH CORPORATION TECHNOLOGIES, INC. Alteration of Name(s) in Register under S187 Assignors: PHILLIPS PETROLEUM CO.
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • C12N15/68Stabilisation of the vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
AU48752/85A 1984-10-30 1985-10-16 Regulatory dna fragment from pichia for heterologous gene expression in yeast Expired AU572002B2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US06/666,391 US4808537A (en) 1984-10-30 1984-10-30 Methanol inducible genes obtained from pichia and methods of use
US06/780,102 US4855231A (en) 1984-10-30 1985-09-25 Regulatory region for heterologous gene expression in yeast
US780102 1985-09-25
US666391 1985-09-25

Publications (2)

Publication Number Publication Date
AU4875285A AU4875285A (en) 1986-06-12
AU572002B2 true AU572002B2 (en) 1988-04-28

Family

ID=27099454

Family Applications (1)

Application Number Title Priority Date Filing Date
AU48752/85A Expired AU572002B2 (en) 1984-10-30 1985-10-16 Regulatory dna fragment from pichia for heterologous gene expression in yeast

Country Status (23)

Country Link
US (1) US4855231A (OSRAM)
EP (2) EP0483115A3 (OSRAM)
JP (5) JP2502508B2 (OSRAM)
KR (1) KR930001117B1 (OSRAM)
CN (1) CN1011243B (OSRAM)
AR (1) AR242988A1 (OSRAM)
AT (1) ATE83263T1 (OSRAM)
AU (1) AU572002B2 (OSRAM)
CA (1) CA1340733C (OSRAM)
DD (1) DD254211A5 (OSRAM)
DE (1) DE3586889T2 (OSRAM)
DK (1) DK496385A (OSRAM)
ES (1) ES8609458A1 (OSRAM)
FI (1) FI94427C (OSRAM)
GR (1) GR852611B (OSRAM)
HU (1) HU205627B (OSRAM)
IE (1) IE930804L (OSRAM)
IL (1) IL76765A (OSRAM)
NO (1) NO178076C (OSRAM)
NZ (1) NZ213842A (OSRAM)
PL (1) PL158965B1 (OSRAM)
PT (1) PT81399B (OSRAM)
YU (1) YU46695B (OSRAM)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU608214B2 (en) * 1986-03-14 1991-03-28 Phillips Petroleum Company Methanol and glucose responsive yeast regulatory regions

Families Citing this family (116)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US4818700A (en) * 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US5032516A (en) * 1985-10-25 1991-07-16 Phillips Petroleum Company Pichia pastoris alcohol oxidase II regulatory region
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
ZA872534B (en) * 1986-05-08 1987-11-25 Phillips Petroleum Co Yeast production of streptokinase
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
US5002876A (en) * 1986-09-22 1991-03-26 Phillips Petroleum Company Yeast production of human tumor necrosis factor
ATE105858T1 (de) * 1987-07-17 1994-06-15 Rhein Biotech Ges Fuer Biotech Dna-moleküle, die für fmdh-kontrollabschnitte und strukturgene für ein protein mit fmdh-aktivität kodieren, sowie deren anwendung.
ES2204884T3 (es) * 1988-01-05 2004-05-01 Roche Diagnostics Gmbh Procedimiento para la obtencion de proteinas o productos genicos que contienen proteinas.
SE8901264L (sv) * 1988-04-11 1989-10-12 Phillips Petroleum Co Uttryck av laxtillvaexthormon i metylotropisk jaest av slaektet pichia
IL89992A0 (en) 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts
IL89993A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of the hiv 24kda gag protein in methylotrophic yeasts
IL89989A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human interleukin-2 in methylotrophic yeasts
NZ228774A (en) * 1988-04-25 1991-05-28 Phillips Petroleum Co Hbv particles containing pres 2 proteins and recombinant processes for their production in yeast cells
IL90021A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Process for the production of interferon
DE3851535T2 (de) * 1988-12-20 1995-02-02 Rhein Biotech Proz & Prod Gmbh Mutanter Stamm von einem methylotrophen Organismus und Verfahren zur Herstellung eines Proteins in methylotrophen Organismus.
DD297841A5 (de) * 1989-02-13 1992-01-23 The Salk Institute Biotechnology/Industrial Associates,Us Herstellung von superoxiddismutase in pichia pastoris hefezellen
GB8924883D0 (en) * 1989-11-03 1989-12-20 Shell Int Research Expression vector and polypeptide synthesis
US5264554A (en) * 1990-01-19 1993-11-23 The Blood Center Of Southeastern Wisconsin, Inc. Platelet cell adhesion molecule and variants thereof
US5369028A (en) * 1990-04-03 1994-11-29 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA and mRNA encoding human neuronal nicotinic acetylcholine receptor compositions and cells transformed with same
US6440681B1 (en) 1990-04-03 2002-08-27 Merck & Co., Inc. Methods for identifying agonists and antagonists for human neuronal nicotinic acetylcholine receptors
US5258302A (en) * 1990-07-03 1993-11-02 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA for expression of aprotinin in methylotrophic yeast cells
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
EP0548267A4 (en) * 1990-09-04 1994-11-23 Salk Inst Biotech Ind Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells
WO1992017595A1 (en) * 1991-04-01 1992-10-15 The Salk Institute Biotechnology/Industrial Associates, Inc. Genes which influence pichia proteolytic activity, and uses therefor
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
US5330901A (en) * 1991-04-26 1994-07-19 Research Corporation Technologies, Inc. Expression of human serum albumin in Pichia pastoris
US5834262A (en) * 1992-01-06 1998-11-10 E. I. Du Pont De Nemours And Company Oxidation of glycolic acid to glyoxylic acid using a microbial cell transformant as catalyst
JP3113435B2 (ja) * 1992-02-28 2000-11-27 サントリー株式会社 メタノールおよび/またはグリセロール添加により誘導可能なプロモーターを有する新規ベクター
US5708141A (en) * 1992-05-11 1998-01-13 Corvas International, Inc. Neutrophil inhibitors
WO1994020631A2 (en) * 1993-03-03 1994-09-15 E.I. Du Pont De Nemours And Company Production of glycolate oxidase in methylotrophic yeast
GB2286397B (en) 1993-03-08 1997-09-10 Salk Inst Biotech Ind Human neuronal nicotinic acetylcholine receptor compositions and methods of employing same
WO1994021802A1 (en) * 1993-03-25 1994-09-29 Biosource Genetics Corporation PICHIA PASTORIS ALCOHOL OXIDASE ZZA1 and $i(ZZA2)
ATE224952T1 (de) * 1993-04-20 2002-10-15 Merck & Co Inc Untereinheiten von menschlichen n-methyl-d- aspartat rezeptoren, diese kodierende nukleinsäure und dessen verwendung
US5521297A (en) 1993-06-04 1996-05-28 Salk Institute Biotechnology/Industrial Associates Nucleic acids encoding human metabotropic glutamate receptors
US5912122A (en) 1993-06-04 1999-06-15 Sibia Neurosciences, Inc. Nucleic acids encoding and method for detecting nucleic acid encoding human metabotropic glutamate receptor subtype mGluR6
US6001581A (en) 1993-06-04 1999-12-14 Sibia Neurosciences, Inc. Cation-based bioassay using human metabotropic glutamate receptors
US6638905B2 (en) * 1993-06-18 2003-10-28 The Salk Institute For Biological Studies Cloning and recombinant production of CFR receptor(s)
US6495343B1 (en) 1993-06-18 2002-12-17 The Salk Institute For Biological Studies Cloning and recombinant production of CRF receptor(s)
GB2287941B (en) * 1993-11-08 1998-01-28 Salk Inst Biotech Ind Human alpha2 neuronal nicotinic acetylcholine receptor compositions and methods employing same
US5453161A (en) * 1993-11-12 1995-09-26 Enichem S.P.A. Polyamic acid to polyimide conversion by microwave heating
EP0788546B9 (en) * 1994-10-18 2007-06-13 Dendreon Corporation Nematode-extracted serine protease inhibitors and anticoagulant proteins
CA2202351A1 (en) * 1994-10-18 1996-04-25 George Phillip Vlasuk Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5872098A (en) * 1995-06-05 1999-02-16 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5866543A (en) * 1995-06-05 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5866542A (en) * 1994-10-18 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5945275A (en) * 1994-10-18 1999-08-31 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5863894A (en) * 1995-06-05 1999-01-26 Corvas International, Inc. Nematode-extracted anticoagulant protein
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6485967B1 (en) 1995-06-07 2002-11-26 Merck & Co., Inc. Human neuronal nicotinic acetylcholine receptor α6 and β3 nucleic acid
CA2237039A1 (en) * 1995-11-09 1997-05-15 Zymogenetics, Inc. Production of gad65 in methylotrophic yeast
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
EP1659173A1 (en) 1997-10-01 2006-05-24 DSMIP Assets B.V. Protein production process
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
EP1025217B1 (en) * 1997-10-24 2006-10-04 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
JP4524816B2 (ja) * 1998-07-06 2010-08-18 東ソー株式会社 Il−6レセプター・il−6直結融合蛋白質
EP1443056B2 (en) * 1998-07-06 2012-08-15 Tosoh Corporation IL-6 receptor.IL-6 direct fusion protein
AU5584999A (en) * 1998-08-28 2000-03-21 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
US6780615B1 (en) 1998-12-31 2004-08-24 Genway Biotech Inc. Production of recombinant monellin using methylotrophic yeast expression system
US6720174B1 (en) 1999-01-28 2004-04-13 Novozymes A/S Phytases
ATE443074T1 (de) * 1999-03-02 2009-10-15 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
US7504253B2 (en) * 1999-06-11 2009-03-17 The Burnham Institute For Medical Research Nucleic acid encoding proteins involved in protein degradation, products and methods related thereof
US6440414B1 (en) * 1999-10-01 2002-08-27 Amgen Inc. Pharmaceutical compositions of fibrinolytic agent
US6261820B1 (en) * 1999-10-01 2001-07-17 Amgen Inc. Fibronolytically active polypeptide
CA2388477C (en) 1999-11-12 2014-01-21 Fibrogen, Inc. Recombinant gelatins with uniform molecular weight
ATE391789T1 (de) 1999-12-10 2008-04-15 Invitrogen Corp Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen
US7033776B2 (en) * 1999-12-17 2006-04-25 Amgen Inc. Method for treatment of indwelling catheter occlusion using fibrinolytic metalloproteinases
WO2001077351A1 (en) * 2000-04-07 2001-10-18 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Vectors and methods for dual protein expression in pichia pastoris and escherichia coli
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
ES2330330T3 (es) * 2000-06-28 2009-12-09 Glycofi, Inc. Procedimiento de produccion de glucoproteinas modificadas.
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
PT1294910E (pt) 2000-06-30 2009-02-16 Vib Vzw Modificação da glicosilação de proteínas em pichia pastoris
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
WO2002081650A2 (en) * 2001-04-05 2002-10-17 The Board Of Regents Of The University Of Nebraska Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast
WO2002095055A2 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US6645739B2 (en) 2001-07-26 2003-11-11 Phoenix Pharmacologies, Inc. Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
EP1497434A4 (en) * 2002-04-15 2005-08-24 Merck & Co Inc Matrix analysis of gene expression in cells (magec)
IL165717A0 (en) 2002-06-26 2006-01-15 Flanders Interuniversity Inst A strain of methylotrophic yeast for producing proteins
US20040204953A1 (en) * 2002-07-17 2004-10-14 Lincoln Muir Subscription based systems, methods and components for providing genomic and proteomic products and services
US7118901B2 (en) * 2002-12-18 2006-10-10 Roche Diagnostics Operations, Inc. Recombinant bovine pancreatic desoxyribonuclease I with high specific activity
DE60319333D1 (de) * 2002-12-20 2008-04-10 Roche Diagnostics Gmbh Hitzelabile Desoxyribonuklease I-Varianten
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
EP1460425A1 (en) * 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Deglycosylated enzymes for conjugates
CN1871351B (zh) * 2003-08-25 2010-06-23 富诺齐梅生物技术股份有限公司 一种新的真菌蛋白及其编码核酸
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
JP2007512838A (ja) 2003-12-01 2007-05-24 インヴィトロジェン コーポレーション 組換え部位を含む核酸分子およびその使用方法
CA2486195C (en) * 2003-12-05 2012-03-06 Stephan Glaser Recombinantly expressed carboxypeptidase b and purification thereof
WO2007031187A1 (en) * 2005-09-14 2007-03-22 Sanofi-Aventis Deutschland Gmbh Cleavage of precursors of insulins by a variant of trypsin
AT504588B1 (de) * 2006-11-16 2008-08-15 Univ Graz Tech Nukleinsäure-promotor
MX2010009679A (es) * 2008-03-03 2010-09-22 Abbott Lab Metodos para transformar la levadura.
KR20110101198A (ko) 2008-12-12 2011-09-15 이 아이 듀폰 디 네모아 앤드 캄파니 재생 가능 자원으로부터의 선형 다이카르복실산의 제조 방법
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
WO2011077359A2 (en) 2009-12-21 2011-06-30 Centre Hospitalier Universitaire Vaudois (Chuv) Synergic action of a prolyl protease and tripeptidyl proteases
LT2710114T (lt) 2011-05-20 2021-12-10 H. Lundbeck A/S Gamyba aukšto grynumo iš keleto subvienetų sudarytų baltymų, tokių kaip antikūnai, transformuotuose mikroorganizmuose, tokiuose kaip pichia pastoris
MX383145B (es) 2011-08-19 2025-03-13 H Lundbeck As Estrategia de multiples copias para produccion de alto titulo y alta pureza de proteinas de multi-subunidades tales como anticuerpos en microbios transformados tal como pichia pastoris.
CN113788884B (zh) 2011-10-07 2024-07-23 隆扎有限公司 可调控启动子
TWI636136B (zh) 2013-03-15 2018-09-21 艾爾德生物製藥股份有限公司 在酵母菌及其它轉型細胞中多肽高產量表現用之溫度變動技術
US9518082B2 (en) 2013-03-15 2016-12-13 Alderbio Holdings Llc Antibody purification and purity monitoring
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
WO2014139608A1 (en) 2013-03-15 2014-09-18 Lonza Ltd Constitutive promoter
ES2891752T3 (es) 2013-03-21 2022-01-31 Evonik Operations Gmbh Purificación de proteínas de triple hélice
KR101957550B1 (ko) 2013-04-30 2019-06-28 도널드 댄포스 플랜트 사이언스 센터 항진균성 식물 단백질, 펩티드 및 사용 방법
US10155793B2 (en) 2013-09-09 2018-12-18 Commonwealth Scientific And Industrial Research Organisation Modified bacterial collagen-like proteins
WO2016120764A1 (en) 2015-01-26 2016-08-04 Centre Hospitalier Universitaire Vaudois (Chuv) Aspergillus oryzae prolyl endopeptidases and use thereof in degradation of polypeptides
US20220220491A1 (en) * 2019-12-19 2022-07-14 Atatürk Üniversitesi Bilimsel Arastirma Projeleri Birimi A method for recombinant protein production

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4875185A (en) * 1984-10-30 1986-06-12 Phillips Petroleum Co. Isolation of a functional gene from pichia yeast
AU4875685A (en) * 1984-10-30 1986-06-12 Research Corporation Technologies, Inc. Transformation of yeasts of the genus pichia
AU563860B2 (en) * 1984-10-30 1987-07-23 Research Corporation Technologies, Inc. Autonomous dna replication sequence from pichia yeast

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK368882A (da) * 1981-08-25 1983-02-26 Alan John Kingsman Expessions vektorer
IE54592B1 (en) * 1982-03-08 1989-12-06 Genentech Inc Anumal interferons, processes involved in their production, compositions containing them, dna sequences coding therefor and espression vehicles containing such sequences and cells transformed thereby
DE3381090D1 (de) * 1982-05-19 1990-02-15 Gist Brocades Nv Klonierungssystem fuer kluyveromyce spezies.
WO1984001153A1 (en) * 1982-09-15 1984-03-29 Collaborative Res Inc Production of interferon in yeast by use of invertase promoter
DE3583194D1 (de) * 1984-07-27 1991-07-18 Unilever Nv Verfahren zur herstellung von einem polypeptid mittels eines transformierten mikroorganismus, ein dazu geeigneter transformierter mikroorganismus, und zur herstellung dieser mikroorganismen geeignete dns sequenzen.

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4875185A (en) * 1984-10-30 1986-06-12 Phillips Petroleum Co. Isolation of a functional gene from pichia yeast
AU4875685A (en) * 1984-10-30 1986-06-12 Research Corporation Technologies, Inc. Transformation of yeasts of the genus pichia
AU563860B2 (en) * 1984-10-30 1987-07-23 Research Corporation Technologies, Inc. Autonomous dna replication sequence from pichia yeast

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU608214B2 (en) * 1986-03-14 1991-03-28 Phillips Petroleum Company Methanol and glucose responsive yeast regulatory regions

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PT81399B (pt) 1987-11-11
PT81399A (en) 1985-11-01
CN1011243B (zh) 1991-01-16
EP0183071A2 (en) 1986-06-04
KR930001117B1 (ko) 1993-02-18
DE3586889T2 (de) 1993-04-15
DK496385A (da) 1986-05-01
US4855231A (en) 1989-08-08
JPH08196275A (ja) 1996-08-06
NO178076B (no) 1995-10-09
JP2552808B2 (ja) 1996-11-13
AR242988A1 (es) 1993-06-30
FI94427C (fi) 1995-09-11
NO178076C (no) 1996-01-17
ES8609458A1 (es) 1986-09-01
HUT38678A (en) 1986-06-30
JPS61173781A (ja) 1986-08-05
EP0483115A2 (en) 1992-04-29
FI854142L (fi) 1986-05-01
YU46695B (sh) 1994-04-05
ATE83263T1 (de) 1992-12-15
PL158965B1 (pl) 1992-10-30
IL76765A (en) 1991-06-30
FI94427B (fi) 1995-05-31
JPH08173161A (ja) 1996-07-09
EP0483115A3 (en) 1992-05-06
DE3586889D1 (de) 1993-01-21
YU169585A (en) 1991-04-30
DK496385D0 (da) 1985-10-29
HU205627B (en) 1992-05-28
JPH06233686A (ja) 1994-08-23
EP0183071A3 (en) 1987-09-23
DD254211A5 (de) 1988-02-17
PL256012A1 (en) 1986-09-23
JP2502508B2 (ja) 1996-05-29
NO854312L (no) 1986-05-02
GR852611B (OSRAM) 1986-03-04
JPH0698789A (ja) 1994-04-12
IE930804L (en) 1986-04-30
FI854142A0 (fi) 1985-10-23
CN85109718A (zh) 1986-08-20
CA1340733C (en) 1999-09-14
KR870003197A (ko) 1987-04-15
AU4875285A (en) 1986-06-12
ES548307A0 (es) 1986-09-01
NZ213842A (en) 1991-11-26
EP0183071B1 (en) 1992-12-09
JP2552807B2 (ja) 1996-11-13

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