AU2020103393A4 - Method for accelerating seed germination and seedling cultivation of Polygonatum cyrtonema - Google Patents
Method for accelerating seed germination and seedling cultivation of Polygonatum cyrtonema Download PDFInfo
- Publication number
- AU2020103393A4 AU2020103393A4 AU2020103393A AU2020103393A AU2020103393A4 AU 2020103393 A4 AU2020103393 A4 AU 2020103393A4 AU 2020103393 A AU2020103393 A AU 2020103393A AU 2020103393 A AU2020103393 A AU 2020103393A AU 2020103393 A4 AU2020103393 A4 AU 2020103393A4
- Authority
- AU
- Australia
- Prior art keywords
- seeds
- polygonatum cyrtonema
- cyrtonema
- furrow
- polygonatum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Botany (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The disclosure provides a method for accelerating seed germination and seedling cultivation
of Polygonatum cyrtonema and relates to the technical field of Polygonatum cultivation. The
disclosure utilizes ultrasound, gibberellin, high dissolved oxygen and a weakly acidic
environment to release the dormancy of P cyrtonema seeds; thus, the P cyrtonema seeds can
emerge neatly within six months, and emergence rate can reach above 90%, effectively
shortening the entire seedling period and improving the seed germination rate and seedling rate of
the P cyrtonema seeds.
Description
[001] The disclosure relates to the technical field of Polygonatum cultivation, and in particular to a method for accelerating seed germination and seedling cultivation of Polygonatum cyrtonema.
[002] Polygonatum sibiricum Red., Polygonatum cyrtonema Hua., and Polygonatum kingianum Coll. et Hemsl.) are perennial herbs of the Liliaceae family, which are included in the Pharmacopoeiaof the People's Republic of China (2015 Edition) as original plants of Rhizoma Polygonati. As edible and medicinal perennial herbs, Rhizoma Polygonati is a traditional bulk medicinal material in China. Rhizoma Polygonati is a traditional Chinese medicinal material. Modern pharmacological studies have shown that saponins of Rhizoma Polygonati, carbohydrates, quinones, amino acids and trace elements contained therein have the functions of immune stimulation, immunopotentiation, anti-aging, anti-virus, and so on. In recent years, as the medicinal and health care value of the Rhizoma Polygonati is increasingly recognized by people, the demand for Polygonatum raw materials is also increasing, and the price continues to rise, which has led to an increase in the predatory collection of wild Polygonatum by mountain farmers. Thus natural resources of Polygonatum are rapidly depleted, which will adversely affect the development, utilization and sustainable development of Polygonatum plants. At present, rhizome asexual reproduction is mostly used in artificial cultivation of Polygonatum, which is a waste of resources and is not conducive to large-scale production and promotion. Long-term asexual reproduction not only causes germplasm degradation, but also makes diseases and insect pests more and more serious, especially soil-bome diseases become more serious as the number of reproductive generations increases.
[003] Application of seed propagation is an effective way to solve soil-borne diseases and reduce production costs. However, P cyrtonema seeds have dormancy characteristics. The natural germination of Polygonatum seeds must undergo two-winter dormancy before they can grow a true leaf. The period between sowing and harvesting lasts for 5-6 years. The method of sand accumulation in winter is now generally used, but there are still shortcomings such as low germination rate and uneven emergence. Therefore, the problem of Polygonatum seedlings has become a bottleneck for artificial large-scale planting. How to release the deep dormancy of Polygonatum seeds and break through the barrier to sexual reproduction of Polygonatum is of great significance to the large-scale and standardized planting of Polygonatum.
[004] In view of this, an objective of the disclosure is to provide a method for accelerating seed germination and seedling cultivation of Polygonatum cyrtonema Hua. (P cyrtonema); thus, the P cyrtonema seeds can emerge neatly within six months, and emergence rate can reach above %, effectively shortening the entire seedling period and improving the seed germination rate and seedling rate of the P cyrtonema seeds.
[005] To achieve the above objective, the present invention provides the following technical solutions.
[006] The disclosure provides a method for accelerating the germination of P cyrtonema seeds, including the following steps: sonicating mature P cyrtonema seeds in an ultrasonic system for 24 h, where the sonication is conducted at a frequency of 20-40 kHz;
[007] the ultrasonic system includes a 50-100 mg/L gibberellin aqueous solution; the ultrasonic system has a pH of 6-6.5, and the ultrasonic system is held at a temperature of 25-35°C; dissolved oxygen content in the ultrasonic system is 8-10 mg/L.
[008] Preferably, the P cyrtonema seeds are seeds after storage.
[009] Preferably, the storage includes storing the mature P cyrtonema seeds harvested in the current year at 4°C until February to March of the following year.
[010] Preferably, a method for obtaining the P cyrtonema seeds includes steps of: harvesting when berries of P cyrtonema turn from green to yellow or black (mature), placing harvested P cyrtonema fruits in an opaque and sealed environment, and fermenting for 7-10 days at 18-25°C; removing peel and pulp of fermented fruits to obtain mature P cyrtonema seeds.
[011] Preferably, a method for removing the peel and pulp of the fermented fruits includes: placing the fermented fruits on a 12-mesh sieve and kneading repeatedly, or mixing the fermented fruits with fine sand and kneading until the peel and the pulp are completely removed, where a volume of the fine sand is 1/3 of a volume of the fermented fruits.
[012] The disclosure further provides a method for cultivating P cyrtonema seedlings, including the following steps: broadcast sowing seeds germinated by the method on a seedling substrate to cultivate P cyrtonema seedlings, where the seedling substrate includes loam or sandy loam.
[013] Preferably, the cultivation is conducted at a temperature of 18-25°C and a humidity of -85%.
[014] Preferably, furrowing on the seedling substrate is further included before the broadcast sowing, where furrow ground is horizontally ditched at a row spacing of 12-15 cm, with a furrow width of 5-6 cm and a furrow depth of 2-3 cm.
[015] Preferably, the broadcast sowing is to sow the seeds into a shallow furrow of furrow ground by 5 x 5 cm.
[016] The disclosure provides a method for accelerating seed germination and seedling cultivation of P cyrtonema, which utilizes ultrasound, gibberellin, high dissolved oxygen and a weakly acidic environment to release the dormancy of P cyrtonema seeds. In the disclosure, ultrasonic wave with high energy can penetrate the lignified cell layer of a P cyrtonema seed, which is conducive to the swelling of the seed, and water can enter the P cyrtonema seed more easily; high dissolved oxygen content in the water is beneficial to the respiration of the seed and the initiation of metabolism; when the P cyrtonema seed starts to breathe, swell and absorb water, absorption of subacid water with gibberellin can increase the activity of enzymes such as amylase and peroxidase in the seed, thereby promoting the seed to break 2-3 years of dormancy and increasing germination rate.
[017] The disclosure provides a method for accelerating the germination of P cyrtonema seeds, including the following steps: sonicating mature P cyrtonema seeds in an ultrasonic system for 24 h, where the sonication is conducted at a frequency of 20-40 kHz;
[018] the ultrasonic system includes a 50-100 mg/L gibberellin aqueous solution; the ultrasonic system has a pH of 6-6.5, and the ultrasonic system is held at a temperature of 25-35°C; dissolved oxygen content in the ultrasonic system is 8-10 mg/L.
[019] In the disclosure, preferably, the mature P cyrtonema seeds are placed in a sonicator, and water is injected into the sonicator. The injection amount of the water is preferably more than 2/3 of the capacity of the sonicator. The disclosure does not specifically limit the source and type of the sonicator. The sonicator selected in examples was purchased from Nanjing Safer Biotech Co., Ltd., and the product model was Biosafer SB25-12DT-22.5L. In the disclosure, gibberellin is added to the water so that the content of gibberellin in the water reaches 50-100 mg/L, and preferably 60-80 mg/L. The disclosure preferably uses 1 mol/L hydrochloric acid or sodium hydroxide to adjust the pH value between 6 and 6.5. In the disclosure, it is preferable to inject oxygen into the water of the sonicator by using an oxygen pump so that the dissolved oxygen content reaches 8-10 mg/L.
[020] P cyrtonema seeds sonicated in the disclosure are preferably seeds after storage, and the storage preferably includes storing mature P cyrtonema seeds harvested in the current year at 4°C until February to March of the following year. In the disclosure, a method for obtaining P cyrtonema seeds preferably includes: harvesting when berries of P cyrtonema turn from green to yellow or black (mature) in September to October each year, placing harvested P cyrtonema fruits in an opaque and sealed environment, and fermenting for 7-10 days at 18-25°C; removing peel and pulp of fermented fruits to obtain mature P cyrtonema seeds. In the disclosure, a method for removing the peel and pulp of the fermented fruits preferably includes: placing the fermented fruits on a 12-mesh sieve and kneading repeatedly, or mixing the fermented fruits with fine sand and kneading until the peel and the pulp are completely removed, where a volume of the fine sand is 1/3 of a volume of the fermented fruits.
[021] The disclosure further provides a method for cultivating P cyrtonema seedlings, including the following steps: broadcast sowing seeds germinated by the method on a seedling substrate to cultivate P cyrtonema seedlings, where the seedling substrate includes loam or sandy loam. After the germination is accelerated, the disclosure preferably further includes washing germinated seeds with clear water. The seedling substrate of the disclosure preferably includes loose and air-permeable sandy loam or loam with more humus. In the disclosure, furrowing is conducted on the seedling substrate, where furrow ground is preferably horizontally ditched at a row spacing of 12-15 cm, with a furrow width of 5-6 cm and a furrow depth of 2-3 cm; the furrow bottom is leveled and slightly compacted, and then seeds are sowed in the furrow. In the disclosure, the spacing between adjacent seeds during sowing is preferably 2 cm; 1 cm thick soil is covered after sowing, and the furrow ground is leveled, kept moist, and prevented from being washed by rain. In the disclosure, water is poured over the soil after the soil is covered, and leaves or straws are covered on the furrow ground; the soil is preferably not exposed. In the cultivation process of the disclosure, it is preferable to control the temperature to be 18-25 and the humidity to be 75-85%; a shade shed is disposed above a seedbed for shading, and weeding is performed every seven days or so. In the shading, shading rate is preferably 70%.
[022] The methods for method for accelerating seed germination and seedling cultivation of P cyrtonema provided by the disclosure will be described in detail below in conjunction with examples, but they should not be construed as limiting the protection scope of the disclosure.
[023] Example 1
1. Seed collection and storage: Mature P cyrtonema fruits were harvested in September, and seeds were harvested from strong mother plants without diseases and insect pests and with full fruits. The harvesting standard for P cyrtonema seeds was: harvesting when berries turn from green to yellow or black (mature). P cyrtonema fruits harvested were placed in an opaque and sealed plastic bag and fermented for 10 days at 25°C. It was preferable that epicarp rotted and became soft and easy to peel off. Well-fermented fruits were placed on a 12-mesh sieve and kneaded repeatedly, or kneaded with about 1/3 volume of fine sand until the peel and the pulp were completely removed. The seeds were rinsed with clear water to remove fine sand and impurities, drained, and stored in a refrigerator at 4°C.
2. Accelerating seed germination: The seeds were taken out in February of the following year, placed in a sonicator (frequency 20 kHz), and mixed with gibberellin (60 mg/L), and the pH value was adjusted between 6 and 6.5 with 1 mol/L hydrochloric acid or sodium hydroxide. Oxygen was passed through by an oxygen pump until the dissolved oxygen content was 8-10 mg/L. The water temperature was 25°C; after 24 h, the seeds were cleaned with running water.
3. Seedling cultivation: Seeds subjected to accelerating germination in step 2 were broadcast sown into a seedling substrate for cultivation. Loose and air-permeable sandy loam or loam with more humus was selected as the substrate for seedling cultivation. A well-made furrow ground was horizontally ditched at a row spacing of 12-15 cm, with a furrow width of 5-6 cm and a furrow depth of 2-3 cm; the spacing between seeds was 2 cm; furrow bottom was leveled and slightly compacted, and then the seeds were sown in a wide furrow; 1 cm thick soil was covered after sowing, and the furrow ground was leveled, kept moist, and prevented from being washed by rain. The temperature during the cultivation process was controlled to be 18-25°C, and the humidity was 75-85%. A shade shed was disposed above a seedbed for shading, and weeding was performed every seven days or so. Germination began around 15 days after accelerating germination and broadcast sowing, and the germination rate reached more than 90%.
[024] Example 2
1. Seed collection and storage: Mature P cyrtonema fruits were harvested in October, and seeds were harvested from strong mother plants without diseases and insect pests and with full fruits. The harvesting standard for P cyrtonema seeds was: harvesting when berries turn from green to yellow or black (mature). P cyrtonema fruits harvested were placed in an opaque and sealed plastic bag and fermented for 10 days at 18°C. It was preferable that epicarp rotted and became soft and easy to peel off. Well-fermented fruits were placed on a 12-mesh sieve and kneaded with about 1/3 volume of fine sand until the peel and the pulp were completely removed. The seeds were rinsed with clear water to remove fine sand and impurities, drained, and stored in a refrigerator at 4°C.
2. Accelerating seed germination: The seeds were taken out in February of the following year, placed in a sonicator (frequency 30 kHz), and mixed with gibberellin (50 mg/L), and the pH value was adjusted between 6 and 6.5 with 1 mol/L hydrochloric acid or sodium hydroxide. Oxygen was passed through by an oxygen pump until the dissolved oxygen content was 8-10 mg/L. The water temperature was 25°C; after 24 h, the seeds were cleaned with running water.
3. Seedling cultivation: Seeds subjected to accelerating germination in step 2 were broadcast sown into a seedling substrate for cultivation. Loose and air-permeable sandy loam or loam with more humus was selected as the substrate for seedling cultivation. A well-made furrow ground was horizontally ditched at a row spacing of 12-15 cm, with a furrow width of 5-6 cm and a furrow depth of 2-3 cm; the spacing between seeds was 2 cm; furrow bottom was leveled and slightly compacted, and then the seeds were sown in a wide furrow; 1 cm thick soil was covered after sowing, and the furrow ground was leveled, kept moist, and prevented from being washed by rain. The temperature during the cultivation process was controlled to be 18-25°C, and the humidity was 75-85%. A shade shed was disposed above a seedbed for shading, and weeding was performed every seven days or so. Germination began around 15 days after accelerating germination and broadcast sowing, and the germination rate reached more than 90%.
[025] Example 3
1. Seed collection and storage: In October, seeds were harvested from strong mother plants without diseases and insect pests and with full fruits. The harvesting standard for P cyrtonema seeds was: harvesting when berries turn from green to yellow or black (mature). Pcyrtonema fruits harvested were placed in an opaque and sealed plastic bag and fermented for 7-10 days at 18-25°C. It was preferable that epicarp rotted and became soft and easy to peel off. Well-fermented fruits were placed on a 12-mesh sieve and kneaded repeatedly until the peel and the pulp were completely removed. The seeds were rinsed with clear water to remove fine sand and impurities, drained, and stored in a refrigerator at 4°C.
2. Accelerating seed germination: The seeds were taken out in March of the following year, placed in a sonicator (frequency 40 kHz), and mixed with gibberellin (80 mg/L), and the pH value was adjusted between 6 and 6.5 with 1 mol/L hydrochloric acid or sodium hydroxide. Oxygen was passed through by an oxygen pump until the dissolved oxygen content was 8-10 mg/L. The water temperature was 30°C; after 24 h, the seeds were cleaned with running water.
3. Seedling cultivation: Seeds subjected to accelerating germination in step 2 were broadcast sown into a seedling substrate for cultivation. Loose and air-permeable sandy loam or loam with more humus was selected as the substrate for seedling cultivation. A well-made furrow ground was horizontally ditched at a row spacing of 12-15 cm, with a furrow width of 5-6 cm and a furrow depth of 2-3 cm; the spacing between seeds was 2 cm; furrow bottom was leveled and slightly compacted, and then the seeds were sown in a wide furrow; 1 cm thick soil was covered after sowing, and the furrow ground was leveled, kept moist, and prevented from being washed by rain. The temperature during the cultivation process was controlled to be 18-25°C, and the humidity was 75-85%. A shade shed was disposed above a seedbed for shading, and weeding was performed every seven days or so. Germination began around 15 days after accelerating germination and broadcast sowing, and the germination rate reached more than 90%.
[026] The foregoing descriptions is merely preferred examples of the disclosure; it should be noted that various modifications and variations can also be made by one of ordinary skill in the art without departing from the principles of the disclosure and fall within the scope of the disclosure.
[027] As used herein and except where the context requires otherwise, the term "comprise" and variations of the term, such as "comprising", "comprises" and "comprised", are not intended to exclude further additions, components, integers or steps.
Claims (5)
1. A method for accelerating the germination of Polygonatum cyrtonema seeds, comprising the following steps: sonicating mature Polygonatum cyrtonema seeds in an ultrasonic system for 24 h, wherein the sonication is conducted at a frequency of 20-40 kHz; the ultrasonic system comprises a 50-100 mg/L gibberellin aqueous solution; the ultrasonic system has a pH of 6-6.5, and the ultrasonic system is held at a temperature of 25-35°C; dissolved oxygen content in the ultrasonic system is 8-10 mg/L.
2. The method according to claim 1, wherein the Polygonatum cyrtonema seeds are seeds after storag; wherein the storage comprises storing mature Polygonatum cyrtonema seeds harvested in the current year at 4°C until February to March of the following year.
3. The method according to claim 1, wherein a method for obtaining the Polygonatum cyrtonema seeds comprises steps of: harvesting when berries of Polygonatum cyrtonema turn from green to yellow or black (mature), placing harvested Polygonatum cyrtonema fruits in an opaque and sealed environment, and fermenting for 7-10 days at 18-25°C; removing peel and pulp of fermented fruits to obtain mature Polygonatum cyrtonema seeds; wherein a method for removing the peel and pulp of the fermented fruits comprises: placing the fermented fruits on a 12-mesh sieve and kneading repeatedly, or mixing the fermented fruits with fine sand and kneading until the peel and the pulp are completely removed, wherein a volume of the fine sand is 1/3 of a volume of the fermented fruits.
4. A method for cultivating Polygonatum cyrtonema seedlings, comprising the following steps: broadcast sowing seeds subjected to accelerating germination by the method according to any one of claims 1 to 3 on a seedling substrate to cultivate Polygonatum cyrtonema seedlings, wherein the seedling substrate comprises loam or sandy loam.
5. The cultivation method according to claim 4, wherein the cultivation is conducted at a temperature of 18-25°C and a humidity of 75-85%; further comprising furrowing on the seedling substrate before the broadcast sowing, wherein furrow ground is horizontally ditched at a row spacing of 12-15 cm, with a furrow width of 5-6 cm and a furrow depth of 2-3 cm; wherein the broadcast sowing is to sow the seeds into a shallow furrow of furrow ground by x 5 cm.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010338484.6 | 2020-04-26 | ||
| CN202010338484.6A CN111316786A (en) | 2020-04-26 | 2020-04-26 | Polygonatum cyrtonema seed germination accelerating and seedling cultivation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2020103393A4 true AU2020103393A4 (en) | 2021-01-28 |
Family
ID=71168223
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2020103393A Ceased AU2020103393A4 (en) | 2020-04-26 | 2020-11-11 | Method for accelerating seed germination and seedling cultivation of Polygonatum cyrtonema |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN111316786A (en) |
| AU (1) | AU2020103393A4 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112997814A (en) * | 2021-02-23 | 2021-06-22 | 贵州省钎汇草堂生物科技有限责任公司 | Large-scale planting method for breeding improved variety with high stress resistance by utilizing wild paris polyphylla tubers |
| CN114128578A (en) * | 2021-11-25 | 2022-03-04 | 重庆市药物种植研究所 | Facility seedling raising method for polygonatum cyrtonema seedlings |
| CN115067159A (en) * | 2022-08-03 | 2022-09-20 | 宁波久久润生物科技有限公司 | Efficient and rapid germination breeding method for polygonatum sibiricum seeds |
| CN115088568A (en) * | 2022-06-09 | 2022-09-23 | 河南盛世中药产业研究院有限公司 | High-yield cultivation method for polygonatum sibiricum by topping and promoting roots |
| CN115191305A (en) * | 2022-08-02 | 2022-10-18 | 江西农业大学 | Method for breaking dormancy and rapid leaf growing of polygonatum zingiberensis seeds |
| CN116711637A (en) * | 2023-06-20 | 2023-09-08 | 中国林业科学研究院亚热带林业实验中心 | Quick seedling forming method for tissue culture seedlings of medicinal plant polygonatum cyrtonema |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115280931B (en) * | 2022-09-02 | 2023-05-19 | 华中农业大学 | Seed treatment method for improving stain resistance |
| CN115885616B (en) * | 2022-11-24 | 2024-04-05 | 浙江省亚热带作物研究所(浙南林业科学研究院) | Application of mycorrhizal fungi in cultivation of rhizoma polygonati seedlings |
| CN116235762A (en) * | 2023-05-12 | 2023-06-09 | 江西农业大学 | Method for rapidly raising seedlings of polygonatum cyrtonema seeds |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104813840B (en) * | 2015-05-23 | 2017-10-10 | 贵州大学 | A kind of fast seedling-cultivating method of David's-harp seed |
| CN107409736A (en) * | 2017-09-12 | 2017-12-01 | 衡阳市九龙生态农业有限公司 | The implantation methods of sealwort |
| CN107926673A (en) * | 2017-12-12 | 2018-04-20 | 云南农业大学 | A kind of breeding method of sealwort seedling |
| CN109089463A (en) * | 2018-08-09 | 2018-12-28 | 重庆市银蜂四云创圆农业开发有限公司 | A kind of polygonatum cyrtonema seed growing method that the service life can be improved |
| CN109197458A (en) * | 2018-09-17 | 2019-01-15 | 安顺市西秀区钰霖种养殖农民专业合作社 | A kind of breeding method of rhizoma polygonati seedling |
| CN109496708A (en) * | 2018-12-19 | 2019-03-22 | 江西省鑫隆农业发展有限公司 | A kind of polygonatum cyrtonema seedling breeding method |
| CN109511313A (en) * | 2019-01-15 | 2019-03-26 | 安徽省科学技术研究院 | A kind of presprouting of seeds agent of polygonatum cyrtonema and its germination accelerating method |
| CN110115131A (en) * | 2019-06-11 | 2019-08-13 | 丽水市农业科学研究院 | A kind of seedling method of polygonatum cyrtonema seed growing |
-
2020
- 2020-04-26 CN CN202010338484.6A patent/CN111316786A/en active Pending
- 2020-11-11 AU AU2020103393A patent/AU2020103393A4/en not_active Ceased
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112997814A (en) * | 2021-02-23 | 2021-06-22 | 贵州省钎汇草堂生物科技有限责任公司 | Large-scale planting method for breeding improved variety with high stress resistance by utilizing wild paris polyphylla tubers |
| CN114128578A (en) * | 2021-11-25 | 2022-03-04 | 重庆市药物种植研究所 | Facility seedling raising method for polygonatum cyrtonema seedlings |
| CN115088568A (en) * | 2022-06-09 | 2022-09-23 | 河南盛世中药产业研究院有限公司 | High-yield cultivation method for polygonatum sibiricum by topping and promoting roots |
| CN115191305A (en) * | 2022-08-02 | 2022-10-18 | 江西农业大学 | Method for breaking dormancy and rapid leaf growing of polygonatum zingiberensis seeds |
| CN115191305B (en) * | 2022-08-02 | 2023-09-15 | 江西农业大学 | Method for breaking dormancy rapid leaf growth of rhizoma polygonati seeds |
| CN115067159A (en) * | 2022-08-03 | 2022-09-20 | 宁波久久润生物科技有限公司 | Efficient and rapid germination breeding method for polygonatum sibiricum seeds |
| CN116711637A (en) * | 2023-06-20 | 2023-09-08 | 中国林业科学研究院亚热带林业实验中心 | Quick seedling forming method for tissue culture seedlings of medicinal plant polygonatum cyrtonema |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111316786A (en) | 2020-06-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2020103393A4 (en) | Method for accelerating seed germination and seedling cultivation of Polygonatum cyrtonema | |
| CN103798020B (en) | A kind of implantation methods of Fructus Luffae | |
| CN106962004B (en) | Polygonatum sibiricum cultivation method for interplanting with sweet corn | |
| CN110226483B (en) | Polygonatum kingianum seedling culture method | |
| CN106069121A (en) | A kind of implantation methods of high-quality Fructus Trichosanthis | |
| CN100376136C (en) | Technology for planting yunnan Dizhu pinellia tube GAP | |
| CN104737665A (en) | Method for germinating polygonatum kingianum seeds | |
| CN104255281A (en) | Sealwort cultivation method | |
| CN103918446B (en) | A kind of domestication implantation methods of wild sealwort | |
| CN104604486A (en) | Peucedanum praeruptorum dunn cultivation method | |
| CN101647354A (en) | Method for cultivating yams | |
| CN105794618A (en) | Perilla-frutescens sprouting vegetable soilless culture method | |
| CN105453753B (en) | Method for treating fritillaria cirrhosa seeds and fritillaria cirrhosa cultivation method | |
| CN106305096A (en) | Planting method of sugarcane | |
| CN105123174A (en) | Cultivation method for baphicanthus cusia bremek | |
| CN108739181A (en) | A kind of efficient implantation methods of bletilla 1+2 | |
| CN104855128A (en) | Platycodon grandiflorus planting method | |
| CN105165539A (en) | High-yield fig planting method based on wide-row close planting technology | |
| CN102523897A (en) | Method for treating reproduced platycodon root seeds | |
| CN107743817A (en) | A kind of method for cultivating hawthorn seedling | |
| CN109197331A (en) | A kind of implantation methods of the wild genuine Radix Glycyrrhizae of intercropping in garden | |
| CN107926396A (en) | A kind of herbicidal methods of planting rhizoma polygonati | |
| CN108651249A (en) | A kind of panax japonicus stereo raise seedling method | |
| CN110338047B (en) | Wild-imitating efficient direct seeding seedling method for bletilla striata seeds | |
| CN107371757A (en) | A kind of implantation methods of selfheal |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGI | Letters patent sealed or granted (innovation patent) | ||
| MK22 | Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry |