CN110338047B - Wild-imitating efficient direct seeding seedling method for bletilla striata seeds - Google Patents

Wild-imitating efficient direct seeding seedling method for bletilla striata seeds Download PDF

Info

Publication number
CN110338047B
CN110338047B CN201910572570.0A CN201910572570A CN110338047B CN 110338047 B CN110338047 B CN 110338047B CN 201910572570 A CN201910572570 A CN 201910572570A CN 110338047 B CN110338047 B CN 110338047B
Authority
CN
China
Prior art keywords
bletilla striata
direct seeding
water
seedling raising
seeds
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201910572570.0A
Other languages
Chinese (zh)
Other versions
CN110338047A (en
Inventor
周燚
江健伟
孙正祥
成胜
方守国
龚天芝
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yangtze University
Original Assignee
Yangtze University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yangtze University filed Critical Yangtze University
Priority to CN201910572570.0A priority Critical patent/CN110338047B/en
Publication of CN110338047A publication Critical patent/CN110338047A/en
Application granted granted Critical
Publication of CN110338047B publication Critical patent/CN110338047B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A01G13/02—Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
    • A01G13/0256—Ground coverings
    • A01G13/0268—Mats or sheets, e.g. nets or fabrics
    • A01G13/0275—Films
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

Abstract

A wild-imitating efficient direct seeding seedling method for bletilla striata seeds is characterized by comprising the following steps: processing bletilla striata fruit pods, establishing a greenhouse and water source system, laying a seedling pool, laying a medium of the seedling pool, sowing, inoculating symbiotic bacteria, managing water and fertilizer in an early stage, managing water and fertilizer in a middle and later stage, carrying out overwintering treatment and domesticating seedlings in the second year. The invention adopts the wild-like direct seeding seedling raising method to greatly improve the germination rate and is suitable for large-scale production.

Description

Wild-imitating efficient direct seeding seedling method for bletilla striata seeds
Technical Field
The invention belongs to the technical field of direct seeding propagation of endangered orchidaceae medicinal plant seeds, and particularly relates to a wild-imitating efficient direct seeding seedling method for bletilla striata seeds.
Background
Bletilla striata (Baletilla striata (Thunb) reichb. f.) also named as desmodium, rhizoma nardostachyos, indocalamus, cymbidium and eriosemum chinense, belongs to the family Orchidaceae, bletilla striata, is taken as a medicine by dry pseudobulb, is mainly produced in the areas such as Yunobu plateau, Yangtze river basin and the like, and is one of the traditional Chinese medicine varieties collected in the traditional Chinese medicine pharmacopoeia. Bletilla has the effects of astringing to stop bleeding, clearing heat and dampness, and reducing swelling and promoting granulation, is widely used for clinically treating hemoptysis and hematemesis, pyocutaneous disease and pyogenic infections, traumatic hemorrhage, chapped skin, pulmonary tuberculosis hemoptysis, ulcer bleeding and the like, and has obvious curative effect. In recent years, the application range of bletilla striata is gradually expanded, and together with the rapid increase of market demand, artificial excessive mining and excavation and serious damage to a habitat cause that wild resources are endangered to be extinct, so that bletilla striata is listed as one of the national important protection wild plants.
At present, the bletilla striata resource in China is scarce and the price is high, and the following reasons are mainly found: (1) the traditional cultivation and plant division breeding efficiency is low; the small-area planting is mainly carried out by the plant division propagation in a split mode, natural propagation and cultivation are carried out from a small amount of seedlings, the scale formation needs more than 6 years and even longer time, and therefore the yield of the bletilla striata at present cannot meet the requirement of large-scale production; (2) although bletilla striata can produce a large number of seeds (0.5-2 ten thousand seeds per pod), the bletilla striata cannot normally germinate under natural conditions because the bletilla striata seeds have no inherent defect of endosperm, the seeds can successfully germinate by virtue of symbiotic bacteria only under the conditions of symbiotic bacteria and proper ecological environment, and the germination rate of the bletilla striata seeds is less than one ten thousand in the natural state; (3) although seedlings can be provided rapidly in large quantities by tissue culture seedling culture, when the seedlings are transplanted to a seedbed by rotating the seedlings to the ground, the solid culture medium carried by the roots is difficult to clean, the seedlings are easy to be infected by germs, and the bletilla is finally dead, so that the transplanting survival rate of the tissue culture seedlings of the bletilla is low, the technical operation is complex, the investment of early-stage equipment is large, and the cost is high; (4) the existing direct seeding and seedling raising technology is immature, substantial breakthrough is not achieved on medium transformation and germination conditions, and in addition, the importance of symbiotic bacteria on bletilla striata seed germination is ignored, so that the seed germination rate is less than 5%; these difficulties result in the supply of seedlings becoming a bottleneck problem for the bletilla striata industry.
Disclosure of Invention
In order to solve the problems, the invention provides a wild-imitating high-efficiency direct seeding seedling method for bletilla striata seeds.
The invention relates to a wild-imitating efficient direct seeding seedling method for bletilla striata seeds, which is characterized by comprising the following steps of:
s1, processing bletilla striata fruit pods: picking the bletilla striata fruit pods with rods when the shells of the bletilla striata fruit pods turn yellow in 9-10 months, drying in the shade, and storing the fruit pods at constant temperature of 4 ℃ for later use when the fruit pods become crisp and the color changes from green to brown;
s2, building a greenhouse and water source system: selecting a field in a water source convenient area to build a steel frame greenhouse, covering an agricultural film outside, covering a sunshade net outside the film, and erecting an atomization micro-spraying pipeline in the greenhouse;
s3, laying a seedling raising pond: ridging and leveling the greenhouse in the step S2 for standby use, leaving operation lines between the ridges, inserting a plurality of bamboo piles at two sides of the ridges, standing a plurality of plastic hollow plates on two sides of the seedling raising compartment by taking the bamboo piles as supports, laying agricultural films in the seedling raising compartment, fixing the agricultural films by the plastic hollow plates at the two sides, and pricking holes in the films at the bottom of the seedling raising compartment;
s4, laying a seedling pond medium: spraying abamectin on the film in the step S3, then paving turfy soil, paving decoction dreg matrix on the turfy soil, spraying water to the seedling pool for thorough watering, and adjusting the pH value of the soil by using acetic acid and citric acid;
s5, sowing: pressing out seeds in the bletilla striata fruit pods, removing pod shells, sieving bletilla striata seed powder, uniformly sowing the sieved bletilla striata seed powder on the compartment surface of the seedling pool in the step S4, and spraying direct seeding nutrient solution I on the compartment surface of the seedling pool in the step S4 the next day after sowing;
s6, inoculating symbiotic bacteria: inoculating bletilla striata symbiotic bacteria to a PDB culture medium, placing at 28 ℃, 200r/min, culturing for 7 days, squeezing and crushing the bacteria liquid, and spraying the bacteria liquid on the surface of a sowed compartment when the bletilla striata is sowed for 10 days and the protocorm with expanded seeds has hairy roots to differentiate;
s7, early-stage water and fertilizer management: spraying water in the morning and evening of each day before the seeds are sown until the seeds germinate and grow second true leaves, spraying a mixed solution of acetic acid and citric acid once every 3 days, and broadcasting carbofuran or 8% toxicant-pungent granules into the greenhouse every 45 days;
s8, management of water and fertilizer in middle and later periods: after 60-75 days, growing a second piece of true leaves from bletilla striata, watering once every 2-3 days by using an atomization sprinkling irrigation device, and spraying once every 7 days by using direct seeding nutrient solution II;
s9, overwintering treatment and seedling domestication in the second year: in 12 months of the current year, after the bletilla striata seedlings in the seedling raising pool are poured, covering pseudobulbs of the bletilla striata on the exposed soil surface with the decoction dreg matrix, keeping the water content of the soil at 35-55%, and preparing for overwintering; in the next year, after the pseudobulb sprouts and comes out of the soil, the direct seeding nutrient solution II is sprayed for 1 time every 10 days.
Further, in the step S2, the length and the width of the greenhouse are 40m and 8m respectively, the sunshade net is 6 needles, the shading rate after the sunshade net covers is 90-95%, the number of the atomization micro-spraying pipelines is 4-5, and a water pump is arranged beside a water source and is connected with the greenhouse atomization system through two disc type filtering systems.
And further, in the step S3, 4 ridges are formed in the greenhouse, the ridge height is 10cm, the ridge width is 1.5m, the operation row width is 50cm, the total length of the bamboo piles is 50cm, 20cm is inserted into the ground, the length of the plastic hollow plate is 4-5 m, the height of the plastic hollow plate is 30m, the thickness of the plastic hollow plate is 4-6 mm, the agricultural film is 8-12 threads and 2.5m, and the average longitudinal and transverse intervals of the film pricking holes at the bottom of the seedling raising chamber are 40 cm.
Still further, in the step S4, 1.8% abamectin diluted by 200 times is sprayed on the film, the thickness of the turfy soil is 8-10 cm, the thickness of the decoction dreg matrix is 3-5 cm, the acetic acid is industrial acetic acid, the citric acid is food-grade citric acid monohydrate, and the pH value of the soil adjusted by the acetic acid and the citric acid is 5.8-6.2.
Further, the peat soil in step S4 is composed of imported peat and northeast peat or peat and peat;
the medicine residue matrix is medicine residues obtained by refining rhizoma polygonati, turmeric, astragalus and liquorice, and the particle size of the medicine residue particles is 0.01-1 mm.
Furthermore, the preparation method of the medicine residue matrix comprises the following steps: fully crushing the medicine residues to 0.01-2 mm, spraying water to the medicine residues, controlling the water content to be 55-65%, mixing 65-70 wt.% of medicine residues and 5-10 wt.% of organic fertilizer, piling the mixture into a conical shape, wherein the height of the conical shape is 1.5-2 m, the width of the conical shape is 2m, covering a plastic film, inoculating EM bacteria, fermenting for 30-45 days, turning over for 1-2 times, and reducing the temperature to 40 ℃ when the mixture is black or blackbrown and is crushed once being kneaded, so that the fermentation is completed.
Further, in step S5, the bletilla striata seed powder is put into a 60 mesh screen and sieved;
the direct seeding nutrient solution I comprises the following components: 50 vol.% of MS liquid culture medium, 5mg/L of NAA naphthylacetic acid, 2mg/L of 6-benzylaminopurine, 0.2 wt.% per mill of glacial acetic acid, 0.5 wt.% per mill of citric acid and 6.0 of pH.
Further, the symbiotic bacteria in step S6 is bletilla striata symbiotic bacteria (Fusarium oxysporum KB3, NCBI website upload data serial number MF 457482);
the bletilla striata symbiotic bacteria separation method comprises the following steps: taking a robust wild bletilla striata root system, fully cleaning, sterilizing and disinfecting, cutting the root system into root slices with the thickness of 0.05-0.5 mm, performing microscopic examination, putting the root slices with the mycelial clusters into a 1.5ml centrifugal tube for mashing, adding 500ul of sterile water for fully shaking to release the mycelial clusters, then coating a root system suspension liquid into a flat plate containing 100ug/ml gentamycin and 17ug/ml chloramphenicol, and picking tip hyphae for purification after the hyphae are produced in the flat plate.
Further, in the step S7, water is sprayed twice a day to keep the water content of the soil at 85-95%, the mass ratio of acetic acid to citric acid in the mixed solution of acetic acid and citric acid is 1:1, the use concentration of the mixed solution is 2 per mill, the pH value of the mixed solution is 5.8-6.2, and the application amount of the carbofuran or 8% toxicant-pungent granules is 300g/667m2。
Further, in step S8, the direct seeding nutrient solution II comprises: 1 wt.% monopotassium phosphate, 0.5 wt.% citric acid, 0.2 wt.% urea and water, pH 6.0;
in the step S8, the atomized sprinkling irrigation device is used for watering once every 2-3 days to keep the water content of the soil at 40-65%;
the thickness of the dreg matrix in the step S9 is 0.5 cm.
The invention has the beneficial effects that: the medium which is favorable for bletilla striata seed germination is used, namely the turmeric dregs used in the layer have neither high nor low nutrients, and are very suitable for bletilla striata seed germination; the lower turfy soil layer can continuously provide later-stage nutrition after the seeds of the bletilla striata germinate; the plastic film is used for laying the bottom, so that the harm of weeds and earthworm mole cricket can be effectively prevented; after bletilla striata seeds are sowed on the compartment surface, citric acid which is a special medicament is used, the medicament and the pH value can be adjusted, ATP energy and a sugar metabolism intermediate skeleton can be provided for plants, and the germination rate can be improved by 6-7 times; meanwhile, bletilla striata symbiotic bacteria are inoculated on the compartment surface, so that nutrition can be effectively provided for the expanded seed balls with hairy roots, the seed balls can be further differentiated into bletilla striata seedlings, the germination rate of bletilla striata seeds is finally greatly improved and is as high as more than 60%, and the seedling raising cost is reduced by 86% compared with that of the traditional method.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the description is intended to be exemplary only, and is not intended to limit the scope of the present invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.
The invention is further explained below with reference to specific examples, but the scope of the invention as claimed is not limited to the following.
The embodiment of the invention is selected from a scientific and technological industry park of Yangtze river university in Jingzhou city, Hubei province, 12 standardized steel-structure plastic greenhouses, the length of each greenhouse is 40 meters, the width of each greenhouse is 8 meters, atomizing sprinkling irrigation facilities are erected in the greenhouse 2.5 meters away from the ground, a 6-needle-density sunshade net is covered outside each greenhouse, the shading rate reaches 90% -95%, 500 ten thousand seedlings are finally grown in a large scale, the germination rate reaches more than 60%, the survival rate is 100%, no pollution is caused, and the seedling raising cost is reduced by 86% compared with that of a traditional method.
Example 1
(1) Seed collection and storage: picking the bletilla striata fruit pods with rods when the shells of the bletilla striata fruit pods turn yellow in 9-10 months, placing the bletilla striata fruit pods in a shade place for 7 days, drying the bletilla striata fruit pods in the shade, enabling the fruit pods to become crisp and the color of the fruit pods to be changed from green to brown, storing the fruit pods in a refrigerator at 4 ℃, crushing the bletilla striata fruit pods by using a vehicle and taking out seeds by using a 35-mesh screen before sowing in 4 months of the next year;
(2) preparing a dreg matrix: fully crushing medicine residues (the granularity is below 0.2 mm), spraying water on administration residues, controlling the water content to be 55%, uniformly mixing materials according to that the medicine residues account for 70% and cow dung accounts for 30%, piling the materials into a cone shape, wherein the height is 1.5-2 m, the width is 2m, and the activity 99 brand EM bacteria is mixed according to the proportion of 1: 10, uniformly inoculating the mixture of wheat bran and sawdust into a material pile, covering a plastic film, fermenting for 40 days, turning over for 1 time every 10 days, and completing fermentation for later use when the material is black or black brown, is broken into pieces without any odor when being pulled or pinched, and the temperature is reduced to 40 ℃.
(3) Preparing a direct seeding nutrient solution: 1/2MS liquid culture medium, NAA naphthylacetic acid 5mg/L, 6-BA 6-benzylaminopurine 2mg/L, mass fraction of glacial acetic acid 0.2 per mill, mass fraction of citric acid 0.5 per mill, adjusting pH to 5.8;
the preparation method of the 1/2MS liquid culture medium per liter comprises the following steps: taking 25ml of macroelement mother liquor, 25ml of calcium salt mother liquor, 10ml of trace element mother liquor, 10ml of organic component mother liquor and 5ml of iron salt mother liquor, and adding distilled water to a constant volume of 1L;
the preparation method of the macroelement mother liquor comprises the following steps: NH (NH)4NO316.5g；MgSO4·7H2O3.7g；KH2PO41.7g；KNO319g, adding water to dissolve, and fixing the volume to 500 ml;
the preparation method of the calcium salt mother liquor comprises the following steps: CaCl23.32g, adding water to dissolve, and fixing the volume to 500 ml;
the preparation method of the microelement mother liquor comprises the following steps: KI0.0415g; na (Na)2MoO4·2H2O0.0125g；H3BO30.31g；CuSO4·5H2O0.00125g；MnSO4·H2O0.845g；CoCl2·6H2O0.00125g；ZnSO4·7H2O0.43g, adding water to dissolve, and fixing the volume to 500 ml;
the preparation method of the ferric salt mother liquor comprises the following steps: 3.725g of EDTA disodium salt, adding 100ml of water, heating and dissolving, FeSO4·7H2O2.785g, adding 100ml of water to dissolve the two, mixing the two evenly, adding water to prepare 500 ml;
the preparation method of the organic component mother solution comprises the following steps: pyridoxine hydrochloride (VB6)0.025 g; thiamine hydrochloride (VB1)0.025 g; 0.025g of nicotinic acid, 0.1g of glycine and 0.1g of inositol are dissolved in water, and the volume is determined to be 500 ml.
(4) Laying a seedling raising pond: 4 ridges are formed in the greenhouse in a whole, the ridge height is 10cm, the ridge width is 1.5m, the greenhouse is leveled for standby application, an operation row with the width of 50cm is reserved between the ridges, bamboo piles with the length of 50cm are inserted into two sides of each ridge (20 cm is inserted underground, 30cm is reserved on the ground), and plastic hollow plates (with the thickness of 4-6 mm, the height of 30cm and the length of 4-5 m) are paved on the bamboo piles. A2.5 m wide agricultural film with the specification of 8-12 filaments is laid on a seedling raising compartment (preventing weeds from growing and preventing underground pests such as earthworm mole crickets from damaging the seedling raising compartment), the agricultural film is pressed on the inner side of the plastic hollow plate, and the plastic hollow plate is covered by the redundant film along the edge and is perpendicular to the outer side. The film at the bottom of the seedling raising compartment is pricked, and the average longitudinal and transverse intervals are 40cm (water filtration is convenient, and water accumulation is prevented).
(5) The method comprises the steps of laying a nursery pond medium, spraying 200 times of 1.8% abamectin on a film (preventing and controlling underground earthworms from penetrating the film to cause harmful seedling raising), laying peat soil with the thickness of 8cm, laying a layer of traditional Chinese medicine residue with the thickness of 3cm on the film (when particles are large, the traditional Chinese medicine residue needs to be fully fermented and then serves as an upper medium), spraying water to the nursery pond to thoroughly pour the mixture, adjusting the pH value of the soil by using industrial acetic acid and food-grade citric acid monohydrate, and adjusting the pH value of the soil to be 5.8.
(6) Sowing: uniformly sowing bletilla striata seeds on the compartment surface of the seedling raising pool by using a 60-mesh sieve until a layer of obvious yellow-white seed powder (about 100g of pure bletilla striata seeds per compartment) appears on the compartment surface of the seedling raising pool, and inoculating by using an electric sprayer to use direct seeding nutrient solution I on the next day after sowing.
(7) Inoculating symbiotic bacteria: inoculating bletilla striata symbiotic bacteria to a PDB culture medium (200 g of potatoes and 20 g of glucose, boiling and filtering, adding water to 1000 ml for sterilization), placing at 28 ℃, 200r/min, culturing for 7 days, squeezing the bacteria liquid by using a hemispherical juicer, diluting by 20 times, and spraying the bacteria liquid on the surface of a sowed compartment by using an electric sprayer about 10 days after sowing to ensure that the symbiotic bacteria can colonize the bletilla striata body through hairy roots to help supply nutrients.
The bletilla striata symbiotic bacteria separation method comprises the following steps: taking robust wild bletilla striata root systems, washing for 20min under tap water, disinfecting for 90S by using 75% alcohol, disinfecting for 3min by using 2% sodium hypochlorite, and rinsing for 3 times by using sterile water; then, cutting the root system into root slices with the thickness of 0.05-0.5 mm by bare-handed slicing; microscopic examination finds that a large number of mycelial clusters exist in cortical cells of root sections, the root sections with the mycelial clusters are placed into a centrifugal tube of 1.5ml to be smashed, 500ul of sterile water is added to fully vibrate and release the mycelial clusters, then root system suspension is coated on a flat plate containing 100ug/ml gentamicin and 17ug/ml chloramphenicol, and after the hyphae are produced in the flat plate, tip hyphae is picked for purification.
(8) Early-stage water and fertilizer management: and sowing the seeds until the seeds germinate and grow second true leaves, and spraying water once in the morning and evening every day by using an atomization spray irrigation device to keep the water content of the soil at 85 percent. Spraying the mixture of acetic acid and citric acid (pH 5.8) once every 3 days, and broadcasting 8% toxic and pungent granule every 45 days to prevent and treat soil insects in the soil.
(9) And (3) water and fertilizer management in middle and later periods: after the second true leaf grows up in 60 days, the spraying irrigation device is used for watering once every 2 days, the direct seeding nutrient solution II (with the pH value of 6.2) is used for spraying once every 7 days, and 8 percent of toxic and pungent granules are scattered into the greenhouse every 45 days to prevent and control underground insects in the soil.
(10) Overwintering treatment and seedling domestication in the second year: in 12 months of the year, after the bletilla striata seedlings in the seedling raising pond are poured, a small amount of decoction dregs matrix is used for covering the bletilla striata pseudobulb on the exposed soil surface, the water content of the soil is kept to be 35%, the bletilla striata pseudobulb is ready to overwinter, and in the next year, after the pseudobulb sprouts out of the soil, direct seeding nutrient solution (1% of potassium dihydrogen phosphate, 0.5% of citric acid and 0.2% of urea and the pH value is 6.0) is sprayed for 1 time every 10 days.
Example 2
(1) Seed collection and storage: picking the bletilla striata fruit pods with rods when the shells of the bletilla striata fruit pods turn yellow in 9-10 months, placing the bletilla striata fruit pods in a shade place for 10 days to dry in the shade, enabling the fruit pods to become crisp and the color of the fruit pods to be changed from green to brown, storing the fruit pods in a refrigerator at 4 ℃, crushing the bletilla striata fruit pods by using a vehicle and taking out seeds by using a 35-mesh screen before sowing in 4 months of the next year;
(2) preparing a dreg matrix: fully crushing medicine residues (the granularity is below 0.2 mm), spraying water on administration residues, controlling the water content to be 65%, uniformly mixing materials according to that the medicine residues account for 70% and cow dung accounts for 30%, piling the materials into a cone shape, wherein the height is 1.5-2 m, the width is 2m, and the activity 99 brand EM bacteria is mixed according to the proportion of 1: 10, uniformly inoculating the mixture of wheat bran and sawdust into a material pile, covering a plastic film, fermenting for 40 days, turning over for 1 time every 10 days, and completing fermentation for later use when the material is black or black brown, is broken into pieces without any odor when being pulled or pinched, and the temperature is reduced to 40 ℃.
(3) Preparing a direct seeding nutrient solution: 1/2MS liquid culture medium, NAA naphthylacetic acid 5mg/L, 6-BA 6-benzylaminopurine 2mg/L, mass fraction of glacial acetic acid 0.2 per mill, mass fraction of citric acid 0.5 per mill, adjusting pH to 6.2;
the preparation method of the 1/2MS liquid culture medium per liter comprises the following steps: taking 25ml of macroelement mother liquor, 25ml of calcium salt mother liquor, 10ml of trace element mother liquor, 10ml of organic component mother liquor and 5ml of iron salt mother liquor, and adding distilled water to a constant volume of 1L;
the preparation method of the macroelement mother liquor comprises the following steps: NH (NH)4NO316.5g；MgSO4·7H2O3.7g；KH2PO41.7g；KNO319g, adding water to dissolve, and fixing the volume to 500 ml;
the preparation method of the calcium salt mother liquor comprises the following steps: CaCl23.32g, adding water to dissolve, and fixing the volume to 500 ml;
the preparation method of the microelement mother liquor comprises the following steps: KI0.0415g; na (Na)2MoO4·2H2O0.0125g；H3BO30.31g；CuSO4·5H2O0.00125g；MnSO4·H2O0.845g；CoCl2·6H2O0.00125g；ZnSO4·7H2O0.43g, adding water to dissolve, and fixing the volume to 500 ml;
the preparation method of the ferric salt mother liquor comprises the following steps: 3.725g of EDTA disodium salt, adding 100ml of water, heating and dissolving, FeSO4·7H2O2.785g, adding 100ml of water to dissolve the two, mixing the two evenly, adding water to prepare 500 ml;
the preparation method of the organic component mother solution comprises the following steps: pyridoxine hydrochloride (VB6)0.025 g; thiamine hydrochloride (VB1)0.025 g; 0.025g of nicotinic acid, 0.1g of glycine and 0.1g of inositol are dissolved in water, and the volume is determined to be 500 ml.
(4) Laying a seedling raising pond: 4 ridges are formed in the greenhouse in a whole, the ridge height is 10cm, the ridge width is 1.5m, the greenhouse is leveled for standby application, an operation row with the width of 50cm is reserved between the ridges, bamboo piles with the length of 50cm are inserted into two sides of each ridge (20 cm is inserted underground, 30cm is reserved on the ground), and plastic hollow plates (with the thickness of 4-6 mm, the height of 30cm and the length of 4-5 m) are paved on the bamboo piles. A2.5 m wide agricultural film with the specification of 8-12 filaments is laid on a seedling raising compartment (preventing weeds from growing and preventing underground pests such as earthworm mole crickets from damaging the seedling raising compartment), the agricultural film is pressed on the inner side of the plastic hollow plate, and the plastic hollow plate is covered by the redundant film along the edge and is perpendicular to the outer side. The film at the bottom of the seedling raising compartment is pricked, and the average longitudinal and transverse intervals are 40cm (water filtration is convenient, and water accumulation is prevented).
(5) The method comprises the steps of laying a nursery pond medium, spraying 200 times of 1.8% abamectin on a film (preventing and controlling underground earthworms from penetrating the film to cause harmful seedling raising), laying peat soil with the thickness of 10cm, laying a layer of traditional Chinese medicine residue with the thickness of 5cm on the film (when particles are large, the traditional Chinese medicine residue needs to be fully fermented and then serves as an upper medium), spraying water to the nursery pond to thoroughly pour the mixture, adjusting the pH value of the soil by using industrial acetic acid and food-grade citric acid monohydrate, and adjusting the pH value of the soil to be 6.2.
(6) Sowing: uniformly sowing bletilla striata seeds on the compartment surface of the seedling raising pool by using a 60-mesh sieve until a layer of obvious yellow-white seed powder (about 100g of pure bletilla striata seeds per compartment) appears on the compartment surface of the seedling raising pool, and inoculating by using an electric sprayer to use direct seeding nutrient solution I on the next day after sowing.
(7) Inoculating symbiotic bacteria: inoculating bletilla striata symbiotic bacteria to a PDB culture medium (200 g of potatoes and 20 g of glucose, boiling and filtering, adding water to 1000 ml for sterilization), placing at 28 ℃, 200r/min, culturing for 7 days, squeezing the bacteria liquid by using a hemispherical juicer, diluting by 20 times, and spraying the bacteria liquid on the surface of a sowed compartment by using an electric sprayer about 10 days after sowing to ensure that the symbiotic bacteria can colonize the bletilla striata body through hairy roots to help supply nutrients.
The bletilla striata symbiotic bacteria separation method comprises the following steps: taking robust wild bletilla striata root systems, washing for 20min under tap water, disinfecting for 90S by using 75% alcohol, disinfecting for 3min by using 2% sodium hypochlorite, and rinsing for 3 times by using sterile water; then, cutting the root system into root slices with the thickness of 0.05-0.5 mm by bare-handed slicing; microscopic examination finds that a large number of mycelial clusters exist in cortical cells of root sections, the root sections with the mycelial clusters are placed into a centrifugal tube of 1.5ml to be smashed, 500ul of sterile water is added to fully vibrate and release the mycelial clusters, then root system suspension is coated on a flat plate containing 100ug/ml gentamicin and 17ug/ml chloramphenicol, and after the hyphae are produced in the flat plate, tip hyphae is picked for purification.
(8) Early-stage water and fertilizer management: and sowing the seeds until the seeds germinate and grow second true leaves, and spraying water once in the morning and evening every day by using an atomization spray irrigation device to keep the water content of the soil at 95 percent. Spraying the mixture of acetic acid and citric acid (pH 5.8) once every 3 days, and broadcasting 8% toxic and pungent granule every 45 days to prevent and treat soil insects in the soil.
(9) And (3) water and fertilizer management in middle and later periods: after the second true leaf grows in 65 days, the second true leaf is watered by using an atomization sprinkling irrigation device once every 2 days, the direct seeding nutrient solution II (with the pH value of 6.2) is sprayed once every 7 days, and 8 percent of toxic and pungent granules are scattered into the greenhouse every 45 days to prevent and control underground insects in soil.
(10) Overwintering treatment and seedling domestication in the second year: in 12 months of the year, after the bletilla striata seedlings in the seedling raising pond are poured, a small amount of decoction dregs matrix is used for covering the bletilla striata pseudobulb on the exposed soil surface, the water content of the soil is kept to be 55%, the bletilla striata pseudobulb is ready to overwinter, and in the next year, after the pseudobulb sprouts out of the soil, direct seeding nutrient solution (1% of potassium dihydrogen phosphate, 0.5% of citric acid and 0.2% of urea and the pH value is 6.0) is sprayed for 1 time every 10 days.
Example 3
(1) Seed collection and storage: picking the bletilla striata fruit pods with rods when the shells of the bletilla striata fruit pods turn yellow in 9-10 months, placing the bletilla striata fruit pods in a shade place for 8 days to dry in the shade, enabling the fruit pods to become crisp and the color of the fruit pods to be changed from green to brown, storing the fruit pods in a refrigerator at 4 ℃, crushing the bletilla striata fruit pods by using a vehicle and taking out seeds by using a 35-mesh screen before sowing in 4 months of the next year;
(2) preparing a dreg matrix: fully crushing medicine residues (the granularity is below 0.2 mm), spraying water on administration residues, controlling the water content to be 60%, uniformly mixing materials according to that the medicine residues account for 70% and cow dung accounts for 30%, piling the materials into a cone shape, wherein the height is 1.5-2 m, the width is 2m, and the activity 99 brand EM bacteria is mixed according to the proportion of 1: 10, uniformly inoculating the mixture of wheat bran and sawdust into a material pile, covering a plastic film, fermenting for 40 days, turning over for 1 time every 10 days, and completing fermentation for later use when the material is black or black brown, is broken into pieces without any odor when being pulled or pinched, and the temperature is reduced to 40 ℃.
(3) Preparing a direct seeding nutrient solution: 1/2MS liquid culture medium, NAA naphthylacetic acid 5mg/L, 6-BA 6-benzylaminopurine 2mg/L, mass fraction of glacial acetic acid 0.2 per mill, mass fraction of citric acid 0.5 per mill, adjusting pH to 6.0;
the preparation method of the 1/2MS liquid culture medium per liter comprises the following steps: taking 25ml of macroelement mother liquor, 25ml of calcium salt mother liquor, 10ml of trace element mother liquor, 10ml of organic component mother liquor and 5ml of iron salt mother liquor, and adding distilled water to a constant volume of 1L;
the preparation method of the macroelement mother liquor comprises the following steps: NH (NH)4NO316.5g；MgSO4·7H2O3.7g；KH2PO41.7g；KNO319g, adding water to dissolve, and fixing the volume to 500 ml;
the preparation method of the calcium salt mother liquor comprises the following steps: CaCl23.32g, adding water to dissolve, and fixing the volume to 500 ml;
the preparation method of the microelement mother liquor comprises the following steps: KI0.0415g; na (Na)2MoO4·2H2O0.0125g；H3BO30.31g；CuSO4·5H2O0.00125g；MnSO4·H2O0.845g；CoCl2·6H2O0.00125g；ZnSO4·7H2O0.43g, adding water to dissolve, and fixing the volume to 500 ml;
the preparation method of the ferric salt mother liquor comprises the following steps: 3.725g of EDTA disodium salt, adding 100ml of water, heating and dissolving, FeSO4·7H2O2.785g, adding 100ml of water to dissolve the two, mixing the two evenly, adding water to prepare 500 ml;
the preparation method of the organic component mother solution comprises the following steps: pyridoxine hydrochloride (VB6)0.025 g; thiamine hydrochloride (VB1)0.025 g; 0.025g of nicotinic acid, 0.1g of glycine and 0.1g of inositol are dissolved in water, and the volume is determined to be 500 ml.
(4) Laying a seedling raising pond: 4 ridges are formed in the greenhouse in a whole, the ridge height is 10cm, the ridge width is 1.5m, the greenhouse is leveled for standby application, an operation row with the width of 50cm is reserved between the ridges, bamboo piles with the length of 50cm are inserted into two sides of each ridge (20 cm is inserted underground, 30cm is reserved on the ground), and plastic hollow plates (with the thickness of 4-6 mm, the height of 30cm and the length of 4-5 m) are paved on the bamboo piles. A2.5 m wide agricultural film with the specification of 8-12 filaments is laid on a seedling raising compartment (preventing weeds from growing and preventing underground pests such as earthworm mole crickets from damaging the seedling raising compartment), the agricultural film is pressed on the inner side of the plastic hollow plate, and the plastic hollow plate is covered by the redundant film along the edge and is perpendicular to the outer side. The film at the bottom of the seedling raising compartment is pricked, and the average longitudinal and transverse intervals are 40cm (water filtration is convenient, and water accumulation is prevented).
(5) The method comprises the steps of laying a nursery pond medium, spraying 200 times of 1.8% abamectin on a film (preventing and controlling underground earthworms from penetrating the film to cause harmful seedling raising), laying 9 cm-thick turfy soil, laying a layer of 4 cm-thick traditional Chinese medicine residues on the film (when particles are large, the traditional Chinese medicine residues need to be fully fermented and then serve as an upper medium), spraying water to the nursery pond to thoroughly pour the traditional Chinese medicine residues, adjusting the pH value of the soil by using industrial acetic acid and food-grade citric acid monohydrate, and adjusting the pH value of the soil to be 6.0.
(6) Sowing: uniformly sowing bletilla striata seeds on the compartment surface of the seedling raising pool by using a 60-mesh sieve until a layer of obvious yellow-white seed powder (about 100g of pure bletilla striata seeds per compartment) appears on the compartment surface of the seedling raising pool, and inoculating by using an electric sprayer to use direct seeding nutrient solution I on the next day after sowing.
(7) Inoculating symbiotic bacteria: inoculating bletilla striata symbiotic bacteria to a PDB culture medium (200 g of potatoes and 20 g of glucose, boiling and filtering, adding water to 1000 ml for sterilization), placing at 28 ℃, 200r/min, culturing for 7 days, squeezing the bacteria liquid by using a hemispherical juicer, diluting by 20 times, and spraying the bacteria liquid on the surface of a sowed compartment by using an electric sprayer about 10 days after sowing to ensure that the symbiotic bacteria can colonize the bletilla striata body through hairy roots to help supply nutrients.
The bletilla striata symbiotic bacteria separation method comprises the following steps: taking robust wild bletilla striata root systems, washing for 20min under tap water, disinfecting for 90S by using 75% alcohol, disinfecting for 3min by using 2% sodium hypochlorite, and rinsing for 3 times by using sterile water; then, cutting the root system into root slices with the thickness of 0.05-0.5 mm by bare-handed slicing; microscopic examination finds that a large number of mycelial clusters exist in cortical cells of root sections, the root sections with the mycelial clusters are placed into a centrifugal tube of 1.5ml to be smashed, 500ul of sterile water is added to fully vibrate and release the mycelial clusters, then root system suspension is coated on a flat plate containing 100ug/ml gentamicin and 17ug/ml chloramphenicol, and after the hyphae are produced in the flat plate, tip hyphae is picked for purification.
(8) Early-stage water and fertilizer management: and sowing the seeds until the seeds germinate and grow second true leaves, and spraying water once in the morning and evening every day by using an atomization spray irrigation device to keep the water content of the soil at 90%. Spraying the mixture of acetic acid and citric acid (pH 5.8) once every 3 days, and broadcasting 8% toxic and pungent granule every 45 days to prevent and treat soil insects in the soil.
(9) And (3) water and fertilizer management in middle and later periods: after the second true leaf grows in 70 days, the spraying irrigation device is used for watering once every 2 days, the direct seeding nutrient solution II (with the pH value of 6.2) is used for spraying once every 7 days, and 8 percent of toxic and pungent granules are scattered into the greenhouse every 45 days to prevent and control underground insects in soil.
(10) Overwintering treatment and seedling domestication in the second year: in 12 months of the year, after the bletilla striata seedlings in the seedling raising pond are poured, a small amount of decoction dregs matrix is used for covering the bletilla striata pseudobulb on the exposed soil surface, the water content of the soil is kept to be 45%, the bletilla striata pseudobulb is ready to overwinter, and in the next year, after the pseudobulb sprouts out of the soil, direct seeding nutrient solution (1% of potassium dihydrogen phosphate, 0.5% of citric acid and 0.2% of urea and the pH value is 6.0) is sprayed for 1 time every 10 days.
It is to be understood that the above-described embodiments of the present invention are merely illustrative of or explaining the principles of the invention and are not to be construed as limiting the invention. Therefore, any modification, equivalent replacement, improvement and the like made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. Further, it is intended that the appended claims cover all such variations and modifications as fall within the scope and boundaries of the appended claims or the equivalents of such scope and boundaries.

Claims (10)

1. A wild-imitating efficient direct seeding seedling method for bletilla striata seeds is characterized by comprising the following steps:
s1, processing bletilla striata fruit pods: picking the bletilla striata fruit pods with rods when the shells of the bletilla striata fruit pods turn yellow in 9-10 months, drying in the shade, and storing the fruit pods at constant temperature of 4 ℃ for later use when the fruit pods become crisp and the color changes from green to brown;
s2, building a greenhouse and water source system: selecting a field in a water source convenient area to build a steel frame greenhouse, covering an agricultural film outside, covering a sunshade net outside the film, and erecting an atomization micro-spraying pipeline in the greenhouse;
s3, laying a seedling raising pond: ridging and leveling the greenhouse in the step S2 for standby use, leaving operation lines between the ridges, inserting a plurality of bamboo piles at two sides of the ridges, standing a plurality of plastic hollow plates on two sides of the seedling raising compartment by taking the bamboo piles as supports, laying agricultural films in the seedling raising compartment, fixing the agricultural films by the plastic hollow plates at the two sides, and pricking holes in the films at the bottom of the seedling raising compartment;
s4, laying a seedling pond medium: spraying abamectin on the film in the step S3, then paving turfy soil, paving decoction dreg matrix on the turfy soil, spraying water to the seedling pool for thorough watering, and adjusting the pH value of the soil by using acetic acid and citric acid;
s5, sowing: pressing out seeds in the bletilla striata fruit pods, removing pod shells, sieving bletilla striata seed powder, uniformly sowing the sieved bletilla striata seed powder on the compartment surface of the seedling pool in the step S4, and spraying direct seeding nutrient solution I on the compartment surface of the seedling pool in the step S4 the next day after sowing;
s6, inoculating symbiotic bacteria: inoculating bletilla striata symbiotic bacteria to a PDB culture medium, placing at 28 ℃, 200r/min, culturing for 7 days, squeezing and crushing the bacteria liquid, and spraying the bacteria liquid on the surface of a sowed compartment when the bletilla striata is sowed for 10 days and the protocorm with expanded seeds has hairy roots to differentiate;
s7, early-stage water and fertilizer management: spraying water in the morning and evening of each day before the seeds are sown until the seeds germinate and grow second true leaves, spraying a mixed solution of acetic acid and citric acid once every 3 days, and broadcasting carbofuran or 8% toxicant-pungent granules into the greenhouse every 45 days;
s8, management of water and fertilizer in middle and later periods: after 60-75 days, growing a second piece of true leaves from bletilla striata, watering once every 2-3 days by using an atomization sprinkling irrigation device, and spraying once every 7 days by using direct seeding nutrient solution II;
s9, overwintering treatment and seedling domestication in the second year: in 12 months of the current year, after the bletilla striata seedlings in the seedling raising pool are poured, covering pseudobulbs of the bletilla striata on the exposed soil surface with the decoction dreg matrix, keeping the water content of the soil at 35-55%, and preparing for overwintering; in the next year, after the pseudobulb sprouts and comes out of the soil, spraying the direct seeding nutrient solution II for 1 time every 10 days;
the residue matrix is the residue extracted from rhizoma Polygonati, rhizoma Dioscoreae Bulbiferae, radix astragali and Glycyrrhrizae radix.
2. The wild-imitating efficient direct seeding seedling raising method for bletilla striata seeds as claimed in claim 1, which is characterized in that: in the step S2, the length and the width of the greenhouse are respectively 40m and 8m, the sunshade net is 6 needles, the shading rate after the sunshade net covers the greenhouse is 90-95%, the number of the atomizing micro-spraying pipelines is 4-5, and a water pump is arranged beside a water source and is connected with a greenhouse atomizing system through two disc type filtering systems.
3. The wild-imitating efficient direct seeding seedling raising method for bletilla striata seeds as claimed in claim 1, which is characterized in that: and S3, 4 ridges are formed in the greenhouse, the ridge height is 10cm, the ridge width is 1.5m, the operation line width is 50cm, the total length of the bamboo piles is 50cm, 20cm is inserted into the ground, the length of the plastic hollow plate is 4-5 m, the height of the plastic hollow plate is 30m, the thickness of the plastic hollow plate is 4-6 mm, the agricultural film is 8-12 threads and 2.5m in specification and width, and the average longitudinal and transverse intervals of the film binding holes at the bottom of the seedling raising compartment are 40 cm.
4. The wild-imitating efficient direct seeding seedling raising method for bletilla striata seeds as claimed in claim 1, which is characterized in that: in the step S4, 1.8% abamectin diluted by 200 times is sprayed on the film, the thickness of the turfy soil is 8-10 cm, the thickness of the decoction dreg matrix is 3-5 cm, the acetic acid is industrial acetic acid, the citric acid is food-grade citric acid monohydrate, and the pH value of the soil adjusted by the acetic acid and the citric acid is 5.8-6.2.
5. The wild-imitating efficient direct seeding seedling raising method for bletilla striata seeds as claimed in claim 4, which is characterized in that: in the step S4, the peat soil is composed of imported peat and northeast peat;
the medicine residue matrix is medicine residues obtained by refining rhizoma polygonati, turmeric, astragalus and liquorice, and the particle size of the medicine residue particles is 0.01-1 mm.
6. The wild-imitating efficient direct seeding seedling raising method for bletilla striata seeds as claimed in claim 4, which is characterized in that: the preparation method of the decoction dreg matrix comprises the following steps: fully crushing the medicine residues to 0.01-2 mm, spraying water to the medicine residues, controlling the water content to be 55-65%, mixing 65-70 wt.% of medicine residues and 5-10 wt.% of organic fertilizer, piling the mixture into a conical shape, wherein the height of the conical shape is 1.5-2 m, the width of the conical shape is 2m, covering a plastic film, inoculating EM bacteria, fermenting for 30-45 days, turning over for 1-2 times, and completing the fermentation when the temperature is reduced to 40 ℃ when the mixture is black or blackish brown and is crushed once being kneaded.
7. The wild-imitating efficient direct seeding seedling raising method for bletilla striata seeds as claimed in claim 1, which is characterized in that: step S5, putting the bletilla striata seed powder into a 60-mesh screen for sieving; the direct seeding nutrient solution I comprises the following components: 50 vol.% of MS liquid culture medium, 5mg/L of NAA naphthylacetic acid, 2mg/L of 6-benzylaminopurine, 0.2 wt.% and thousandths of glacial acetic acid, 0.5 wt.% and thousandths of citric acid and pH 6.0.
8. The wild-imitating efficient direct seeding seedling raising method for bletilla striata seeds as claimed in claim 1, which is characterized in that: the symbiotic bacteria in the step S6 are bletilla striata symbiotic bacteria, and the NCBI website uploads a data serial number MF 457482; the bletilla striata symbiotic bacteria separation method comprises the following steps: taking a robust wild bletilla striata root system, fully cleaning, sterilizing and disinfecting, cutting the root system into root slices with the thickness of 0.05-0.5 mm, performing microscopic examination, putting the root slices with the mycelial clusters into a 1.5ml centrifugal tube for mashing, adding 500ul of sterile water for fully shaking to release the mycelial clusters, then coating a root system suspension liquid into a flat plate containing 100ug/ml gentamycin and 17ug/ml chloramphenicol, and picking tip hyphae for purification after the hyphae are produced in the flat plate.
9. The wild-imitating efficient direct seeding seedling raising method for bletilla striata seeds as claimed in claim 1, which is characterized in that: in the step S7, water is sprayed twice a day to keep the water content of the soil at 85-95%, the mass ratio of acetic acid to citric acid in the mixed solution of acetic acid and citric acid is 1:1, the use concentration of the mixed solution is 2 per thousand, the pH value of the mixed solution is 5.8-6.2, and the application amount of carbofuran or 8% toxicant-pungent granules is 300g/667m2。
10. The wild-imitating efficient direct seeding seedling raising method for bletilla striata seeds as claimed in claim 1, which is characterized in that: in the step S8, the direct seeding nutrient solution II comprises the following components: 1 wt.% monopotassium phosphate, 0.5 wt.% citric acid, 0.2 wt.% urea and water, pH 6.0;
in the step S8, the atomized sprinkling irrigation device is used for watering once every 2-3 days to keep the water content of the soil at 40-65%;
the thickness of the dreg matrix in the step S9 is 0.5 cm.
CN201910572570.0A 2019-06-28 2019-06-28 Wild-imitating efficient direct seeding seedling method for bletilla striata seeds Active CN110338047B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910572570.0A CN110338047B (en) 2019-06-28 2019-06-28 Wild-imitating efficient direct seeding seedling method for bletilla striata seeds

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910572570.0A CN110338047B (en) 2019-06-28 2019-06-28 Wild-imitating efficient direct seeding seedling method for bletilla striata seeds

Publications (2)

Publication Number Publication Date
CN110338047A CN110338047A (en) 2019-10-18
CN110338047B true CN110338047B (en) 2021-09-17

Family

ID=68176844

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910572570.0A Active CN110338047B (en) 2019-06-28 2019-06-28 Wild-imitating efficient direct seeding seedling method for bletilla striata seeds

Country Status (1)

Country Link
CN (1) CN110338047B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113207590A (en) * 2021-04-29 2021-08-06 南京工业大学大丰海洋产业研究院 Bletilla direct seeding and seedling raising method utilizing symbiotic bacteria

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105103714A (en) * 2015-08-25 2015-12-02 长江大学 Manual germination accelerating method and seedling raising method of Bletilla sfriata (Thunb.)Reiehb.f.
CN105103924A (en) * 2015-09-25 2015-12-02 马龙县兴裕农业生物科技开发有限公司 High-yield cultivation method for pollution-free bletilla striata
CN105379551A (en) * 2015-12-24 2016-03-09 陕西师范大学 Bletilla ochracea direct-seeded seedling transplanting method
CN105659979A (en) * 2016-01-18 2016-06-15 云南绿辰生物科技开发有限公司 Method for cultivating symbiotic bacteria of Bletilla striata
CN107371710A (en) * 2017-08-08 2017-11-24 云南农业大学 A kind of bletilla seed sowing two-stages seeding method
CN109122157A (en) * 2018-08-28 2019-01-04 十堰市农业科学院 A kind of fertilizing method promoting live streaming seedling fast-growth in bletilla striata greenhouse

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105103714A (en) * 2015-08-25 2015-12-02 长江大学 Manual germination accelerating method and seedling raising method of Bletilla sfriata (Thunb.)Reiehb.f.
CN105103924A (en) * 2015-09-25 2015-12-02 马龙县兴裕农业生物科技开发有限公司 High-yield cultivation method for pollution-free bletilla striata
CN105379551A (en) * 2015-12-24 2016-03-09 陕西师范大学 Bletilla ochracea direct-seeded seedling transplanting method
CN105659979A (en) * 2016-01-18 2016-06-15 云南绿辰生物科技开发有限公司 Method for cultivating symbiotic bacteria of Bletilla striata
CN107371710A (en) * 2017-08-08 2017-11-24 云南农业大学 A kind of bletilla seed sowing two-stages seeding method
CN109122157A (en) * 2018-08-28 2019-01-04 十堰市农业科学院 A kind of fertilizing method promoting live streaming seedling fast-growth in bletilla striata greenhouse

Also Published As

Publication number Publication date
CN110338047A (en) 2019-10-18

Similar Documents

Publication Publication Date Title
CN104094750B (en) Pollution-free food cabbage mustard cultivation method
CN104094749A (en) Cultivation method of green food celery
CN103283452B (en) Radix codonopsis cultivation method
CN105284393A (en) Method for seedling sealwort seeds
CN103858724A (en) Breeding method for high-quality walnut seedlings
CN105145104A (en) Method for segmenting bletilla striata tubers to perform seedling propagation and raising
CN105900723B (en) A kind of method for culturing seedlings and cultural method of panax japonicus majoris
CN104025892A (en) Suberect spatholobus stem cultivation method
CN106561428A (en) Soilless culture method for pumpkins
CN106986691A (en) A kind of cultural method of spun gold emperor chrysanthemum
CN105393736A (en) Planting method for semen cassia
CN109644805A (en) A kind of method of Radix Salviae Miltiorrhizae root segment plantation
CN104488492B (en) Large-scale production method for Weining codonopsis pilosula
CN109076915A (en) A kind of propagation method of Nanchuan lily
CN105453752A (en) Method for promoting rapid germination of rhizoma paridis seeds
CN104221701A (en) Ford nervilia leaf artificial cultivation method
CN107079693A (en) A kind of facilities vegetable implantation methods with insect resistant effect
CN102792831B (en) High-efficiency rapid propagation technique for Chinese yew
CN106358664A (en) Method for sexually breeding Bletilla striata seedlings on large scale by simulating natural conditions
CN110338047B (en) Wild-imitating efficient direct seeding seedling method for bletilla striata seeds
CN105123174A (en) Cultivation method for baphicanthus cusia bremek
CN106613172B (en) Original ecological planting method of anoectochilus roxburghii in Guangdong-oriented chemical-change lake sky and land
CN106068986A (en) A kind of salt-soda soil method for growing vegetables
CN111226722A (en) Konjak and pinellia ternate intercropping cultivation method
CN106818434A (en) A kind of natural propagation method of Seeds of Dendrobium Candidum bed sowing

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB03 Change of inventor or designer information

Inventor after: Zhou Yi

Inventor after: Jiang Jianwei

Inventor after: Sun Zhengxiang

Inventor after: Cheng Sheng

Inventor after: Fang Shouguo

Inventor after: Gong Tianzhi

Inventor before: Zhou Yi

Inventor before: Jiang Jianwei

Inventor before: Sun Zhengxiang

Inventor before: Cheng Sheng

Inventor before: Fang Shouguo

Inventor before: Gong Tianzhi

CB03 Change of inventor or designer information
GR01 Patent grant
GR01 Patent grant