CN112997814A - Large-scale planting method for breeding improved variety with high stress resistance by utilizing wild paris polyphylla tubers - Google Patents
Large-scale planting method for breeding improved variety with high stress resistance by utilizing wild paris polyphylla tubers Download PDFInfo
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- CN112997814A CN112997814A CN202110201463.4A CN202110201463A CN112997814A CN 112997814 A CN112997814 A CN 112997814A CN 202110201463 A CN202110201463 A CN 202110201463A CN 112997814 A CN112997814 A CN 112997814A
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- 241000244987 Daiswa polyphylla Species 0.000 title claims abstract description 89
- 238000000034 method Methods 0.000 title claims abstract description 33
- 238000009395 breeding Methods 0.000 title claims abstract description 18
- 230000001488 breeding effect Effects 0.000 title claims abstract description 17
- 201000010099 disease Diseases 0.000 claims abstract description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 11
- 238000003306 harvesting Methods 0.000 claims abstract description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 11
- 239000002689 soil Substances 0.000 claims description 30
- 241000196324 Embryophyta Species 0.000 claims description 28
- 239000003895 organic fertilizer Substances 0.000 claims description 17
- 239000010902 straw Substances 0.000 claims description 17
- 239000003337 fertilizer Substances 0.000 claims description 15
- 238000002791 soaking Methods 0.000 claims description 14
- 210000003608 fece Anatomy 0.000 claims description 13
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 11
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 11
- 239000006013 carbendazim Substances 0.000 claims description 11
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 10
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 10
- 235000011613 Pinus brutia Nutrition 0.000 claims description 10
- 241000018646 Pinus brutia Species 0.000 claims description 10
- 241000607479 Yersinia pestis Species 0.000 claims description 10
- 210000004209 hair Anatomy 0.000 claims description 10
- 238000009333 weeding Methods 0.000 claims description 10
- 229930191978 Gibberellin Natural products 0.000 claims description 9
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 9
- 239000003448 gibberellin Substances 0.000 claims description 9
- 239000004576 sand Substances 0.000 claims description 9
- 241001494479 Pecora Species 0.000 claims description 7
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 5
- 241000238631 Hexapoda Species 0.000 claims description 5
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 5
- 241000209140 Triticum Species 0.000 claims description 5
- 235000021307 Triticum Nutrition 0.000 claims description 5
- 235000012255 calcium oxide Nutrition 0.000 claims description 5
- 239000000292 calcium oxide Substances 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 239000004571 lime Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000007790 scraping Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 238000009394 selective breeding Methods 0.000 claims 8
- 240000007594 Oryza sativa Species 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 238000002054 transplantation Methods 0.000 abstract 1
- 241000283690 Bos taurus Species 0.000 description 6
- 230000035784 germination Effects 0.000 description 6
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 241000209094 Oryza Species 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000234280 Liliaceae Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention relates to the technical field of paris polyphylla breeding, in particular to a large-scale planting method for breeding high-stress-resistance improved varieties by utilizing the breeding of wild paris polyphylla tubers, which comprises the following steps: tuber selection, tuber disease-resistant treatment, tuber growth treatment, tuber dicing treatment, disinfection, seedling raising, growth management, transplantation, paris polyphylla growth and harvesting.
Description
Technical Field
The invention relates to the technical field of paris polyphylla breeding, in particular to a large-scale planting method for breeding high-stress-resistance improved varieties by utilizing tuber breeding of wild paris polyphylla.
Background
Rhizoma paridis, named as Chinese medicine, is dried rhizome of Yunnan rhizoma paridis or Paris polyphylla Smith of Liliaceae. It is bitter in taste and slightly cold in nature; the wild paris polyphylla is usually bred to obtain a good variety in order to meet the requirements of medicinal materials in the cultivation process, so that large-scale planting at the later stage is facilitated to meet the medicinal requirements, but the existing breeding mode cannot breed the good variety with high stress resistance, and the stress resistance of the plants refers to certain characters of resisting adverse environments; for example, the paris polyphylla cultivated by the existing wild paris polyphylla breeding method has no high stress resistance, so that a large-scale planting method for cultivating high-stress-resistance improved varieties by breeding tubers of the wild paris polyphylla is provided.
Disclosure of Invention
The invention aims to solve the defects in the background art, and provides a large-scale planting method for breeding improved varieties with high stress resistance by using the tuber of wild paris polyphylla.
In order to achieve the above purposes, the technical scheme adopted by the invention is as follows: the method for breeding and cultivating the high stress resistance improved variety in large scale by utilizing the tuber of the wild paris polyphylla comprises the following steps:
s1, tuber selection: selecting strong-growing wild rhizoma paridis tubers with a growth period of more than 4 years and no plant diseases and insect pests as seedling seeds;
s2, tuber disease-resistant treatment: mixing the tuber of the paris polyphylla with clean fine sand, slightly scraping the outer skin, generating a plurality of spot-shaped skin-broken parts on the surface of the tuber, fishing out, cleaning, and soaking in 1000 times of 50 percent carbendazim solution for 2-3 minutes;
s3, tuber growth treatment: fishing out the tubers in the step S2, and soaking the tubers in gibberellin solution with the mass percentage concentration of 0.5-0.8% for 6-10 hours at the temperature of 20-25 ℃;
s4, tuber dicing treatment: cutting the tubers into blocks;
s5, disinfection: covering quicklime on the cut wound and the scratched outer skin of the cut paris polyphylla for disinfection;
s6, seedling culture: building a sun-shading greenhouse, and transplanting tubers into the sun-shading greenhouse;
s7, growth management: keeping the soil moisture in the greenhouse to be more than 80% from the beginning of seedling culture until the end of seedling culture;
s8, transplanting: transplanting the rooted and germinated tubers into a field for culture after 2 years;
s9, growth and harvesting of paris polyphylla: and (3) producing the paris polyphylla and harvesting seeds in autumn after transplanting for 2-3 years to obtain paris polyphylla seeds with high stress resistance and good seeds.
Preferably, the shading degree of the greenhouse in the step S6 is controlled to be 50-70%, the planting ground is transversely ditched, the seedlings are planted according to the line spacing of 15-20cm and the plant spacing of 15-20cm, after the seedlings are transplanted into the sun-shading greenhouse, base fertilizer is applied for 3cm in thickness, the soil is covered by 6cm in thickness, surface fertilizer is applied for 3cm in thickness, and the soil surface is covered by straws, wheat straws or pine hairs.
Preferably, in step S6, leaves of the paris polyphylla seedling are completely differentiated in morphological state, and are cut at a position 3-5cm below the first round leaves of the seedling.
Preferably, in the step S8, the planting is carried out according to the row spacing of 25-30cm and the plant spacing of 15-20cm, base fertilizer is applied for 3cm before the transplanting, the soil surface is covered with pine hair for water retention after the planting is finished so as to prevent the soil from becoming tight due to watering, and a shading net is built.
Preferably, in step S9, organic fertilizer with a thickness of 5cm is scattered on the soil surface after the stalks wither every 10 months during the growth of the paris polyphylla.
Preferably, the organic fertilizer comprises 35% of cow dung, 25% of sheep dung, 15% of plant ash and 25% of straws in percentage by weight, and is prepared by stirring the components and then fermenting at normal temperature for half a year.
Preferably, in step S9, fertilization, weeding and pest control are performed during the growth of the paris polyphylla, wherein weeding is performed before weeds grow to three times of leaves, and the planting area is disinfected by lime uniformly.
Preferably, the selection of the wild paris polyphylla tubers in step S1 is performed in a winter weather with an air temperature of less than 10 ℃.
Preferably, the rootstocks at a position 4cm below the terminal bud in the division in step S4 are transversely cut into seedlings with terminal buds, and the seedlings are cut into 4 cm-long block structures.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the method, wild rhizoma paridis tubers are selected in winter, anti-freezing wild tubers are selected, meanwhile, screening and transplanting are carried out through seedling cultivation management for 2 years, screening and cultivation for 2-3 years are carried out after transplanting, rhizoma paridis is screened again, and finally obtained rhizoma paridis seeds are all fine seeds with high stress resistance screened layer by layer.
2. Soaking treatment is carried out on the paris polyphylla tubers through carbendazim, the carbendazim effectively improves the antibacterial effect of the tubers, reduces the infection risk of fungi, and simultaneously increases the contact effect of the tubers and the carbendazim by a mode of rubbing the surfaces of the paris polyphylla tubers through fine sand, so that the tubers have a better antibacterial effect, and the survival rate of cultivation is increased.
3. The method has the advantages that the elongation of cells can be accelerated by soaking the tuber of the paris polyphylla in gibberellin, the division of the cells is promoted, the expansion of the cells is promoted, the surface of the tuber of the paris polyphylla is broken through fine sand, the cells in the tuber are promoted to be soaked in the gibberellin, the germination of the tuber is promoted, the germination time of the tuber of the wild paris polyphylla is shortened, and the germination rate of the wild paris polyphylla is improved.
4. In the preferred scheme, the paris polyphylla is fertilized when growing, and the organic fertilizer utilizes mountain cattle, sheep manure, plant ash, straw resources, has reached green, economy, environmental protection's purpose, and the organic fertilizer through fermentation for a long time, has improved the absorption rate of paris polyphylla to the organic fertilizer, has promoted the growth of paris polyphylla.
Detailed Description
The following description is presented to disclose the invention so as to enable any person skilled in the art to practice the invention. The preferred embodiments in the following description are given by way of example only, and other obvious variations will occur to those skilled in the art.
Example 1
The method for breeding and cultivating the high stress resistance improved variety in large scale by utilizing the tuber of the wild paris polyphylla comprises the following steps:
s1, tuber selection: selecting strong-growing wild rhizoma paridis tubers with a growth period of more than 4 years and no plant diseases and insect pests as seedling seeds;
s2, tuber disease-resistant treatment: mixing the tuber of the paris polyphylla with clean fine sand, slightly scraping the outer skin, generating a plurality of spot-shaped skin-broken parts on the surface of the tuber, fishing out, cleaning, and soaking in 1000 times of 50 percent carbendazim solution for 2 minutes;
s3, tuber growth treatment: fishing out the tubers in the step S2, and soaking the tubers in gibberellin solution with the mass percentage concentration of 0.5% for 6 hours at the temperature of 20 ℃;
s4, tuber dicing treatment: cutting the tubers into blocks;
s5, disinfection: covering quicklime on the cut wound and the scratched outer skin of the cut paris polyphylla for disinfection;
s6, seedling culture: building a sun-shading greenhouse, and transplanting tubers into the sun-shading greenhouse;
s7, growth management: keeping the soil moisture in the greenhouse to be more than 80% from the beginning of seedling culture until the end of seedling culture;
s8, transplanting: transplanting the rooted and germinated tubers into a field for culture after 2 years;
s9, growth and harvesting of paris polyphylla: and (3) producing the paris polyphylla and harvesting seeds in autumn after transplanting for 2 years to obtain paris polyphylla seeds with high stress resistance and good seeds.
And S6, controlling the shading degree of the greenhouse to be 50%, transversely ditching on the planting ground, planting according to the row spacing of 15cm and the plant spacing of 15cm during planting, transplanting the seedlings into the shading greenhouse, applying base fertilizer to the seedlings for 3cm, covering the seedlings with soil for 6cm, applying surface fertilizer to the seedlings for 3cm, and covering the soil surface with rice straws, wheat straws or pine hair.
And S6, cutting off the leaves of the paris polyphylla at a position 3cm below the first round leaves of the paris polyphylla after the leaves of the paris polyphylla are completely differentiated.
And step S8, planting according to the row spacing of 25cm and the plant spacing of 15cm during transplanting, applying base fertilizer of 3cm before transplanting, covering the surface of the soil with pine hair for water retention after burying to prevent the soil from becoming tight due to watering, and building a shading net.
In step S9, organic fertilizer with the thickness of 5cm is scattered on the soil surface after the stalks wither every 10 months in the growth process of the paris polyphylla.
The organic fertilizer comprises 35% of cow dung, 25% of sheep dung, 15% of plant ash and 25% of straws by weight percent, and is prepared by stirring the components and fermenting at normal temperature for half a year.
And step S9, fertilizing, weeding and pest control are carried out in the growth process of the paris polyphylla, wherein weeding is carried out before weeds grow to three times of leaves, and the planting area is uniformly disinfected by lime.
And in the step S1, selecting the wild rhizoma paridis tuber in the weather with the air temperature less than 10 ℃ in winter.
In the step S4, the rootstock is transversely cut into pieces 4cm below the terminal bud as seedlings with the terminal bud, and the pieces are cut into block structures 4cm in length.
Example 2
The method for breeding and cultivating the high stress resistance improved variety in large scale by utilizing the tuber of the wild paris polyphylla comprises the following steps:
s1, tuber selection: selecting strong-growing wild rhizoma paridis tubers with a growth period of more than 4 years and no plant diseases and insect pests as seedling seeds;
s2, tuber disease-resistant treatment: mixing the tuber of the paris polyphylla with clean fine sand, slightly scraping the outer skin, generating a plurality of spot-shaped skin-broken parts on the surface of the tuber, fishing out, cleaning, and soaking in 1000 times of 50 percent carbendazim solution for 3 minutes;
s3, tuber growth treatment: fishing out the tubers in the step S2, and soaking the tubers at 25 ℃ for 10 hours by using a gibberellin solution with the mass percentage concentration of 0.8%;
s4, tuber dicing treatment: cutting the tubers into blocks;
s5, disinfection: covering quicklime on the cut wound and the scratched outer skin of the cut paris polyphylla for disinfection;
s6, seedling culture: building a sun-shading greenhouse, and transplanting tubers into the sun-shading greenhouse;
s7, growth management: keeping the soil moisture in the greenhouse to be more than 80% from the beginning of seedling culture until the end of seedling culture;
s8, transplanting: transplanting the rooted and germinated tubers into a field for culture after 2 years;
s9, growth and harvesting of paris polyphylla: and (4) producing the paris polyphylla and harvesting seeds in autumn after transplanting for 3 years to obtain paris polyphylla seeds with high stress resistance and good seeds.
And S6, controlling the shading degree of the greenhouse at 70%, transversely ditching on the planting ground, planting according to the row spacing of 20cm and the plant spacing of 20cm during planting, transplanting the seedlings into the shading greenhouse, applying base fertilizer 3cm thick, covering soil 6cm thick, applying surface fertilizer 3cm thick, and covering the soil surface with rice straws, wheat straws or pine hair.
And S6, cutting off the leaves of the paris polyphylla 5cm below the first round leaves.
And step S8, planting according to the row spacing of 30cm and the plant spacing of 20cm during transplanting, applying base fertilizer of 3cm before transplanting, covering the surface of the soil with pine hair for water retention after burying to prevent the soil from becoming tight due to watering, and building a shading net.
In step S9, organic fertilizer with the thickness of 5cm is scattered on the soil surface after the stalks wither every 10 months in the growth process of the paris polyphylla.
The organic fertilizer comprises 35% of cow dung, 25% of sheep dung, 15% of plant ash and 25% of straws by weight percent, and is prepared by stirring the components and fermenting at normal temperature for half a year.
And step S9, fertilizing, weeding and pest control are carried out in the growth process of the paris polyphylla, wherein weeding is carried out before weeds grow to three times of leaves, and the planting area is uniformly disinfected by lime.
And in the step S1, selecting the wild rhizoma paridis tuber in the weather with the air temperature less than 10 ℃ in winter.
In the step S4, the rootstock is transversely cut into pieces 4cm below the terminal bud as seedlings with the terminal bud, and the pieces are cut into block structures 4cm in length.
Example 3
The method for breeding and cultivating the high stress resistance improved variety in large scale by utilizing the tuber of the wild paris polyphylla comprises the following steps:
s1, tuber selection: selecting strong-growing wild rhizoma paridis tubers with a growth period of more than 4 years and no plant diseases and insect pests as seedling seeds;
s2, tuber disease-resistant treatment: mixing the tuber of the paris polyphylla with clean fine sand, slightly scraping the outer skin, generating a plurality of spot-shaped skin-broken parts on the surface of the tuber, fishing out, cleaning, and soaking in 1000 times of 50 percent carbendazim solution for 2 minutes;
s3, tuber growth treatment: fishing out the tubers in the step S2, and soaking the tubers at the temperature of 22 ℃ for 8 hours by using a gibberellin solution with the mass percentage concentration of 0.7%;
s4, tuber dicing treatment: cutting the tubers into blocks;
s5, disinfection: covering quicklime on the cut wound and the scratched outer skin of the cut paris polyphylla for disinfection;
s6, seedling culture: building a sun-shading greenhouse, and transplanting tubers into the sun-shading greenhouse;
s7, growth management: keeping the soil moisture in the greenhouse to be more than 80% from the beginning of seedling culture until the end of seedling culture;
s8, transplanting: transplanting the rooted and germinated tubers into a field for culture after 2 years;
s9, growth and harvesting of paris polyphylla: and (4) producing the paris polyphylla and harvesting seeds in autumn after transplanting for 3 years to obtain paris polyphylla seeds with high stress resistance and good seeds.
And S6, controlling the shading degree of the greenhouse at 60%, transversely ditching on the planting ground, planting according to the row spacing of 18cm and the plant spacing of 18cm during planting, transplanting the seedlings into the shading greenhouse, applying base fertilizer 3cm thick, covering soil 6cm thick, applying surface fertilizer 3cm thick, and covering the soil surface with rice straws, wheat straws or pine hair.
In step S6, cutting off the leaves of the paris polyphylla at a position 4cm below the first round leaves of the seedlings after the leaves of the paris polyphylla are completely differentiated.
And step S8, planting according to the row spacing of 28cm and the plant spacing of 18cm during transplanting, applying base fertilizer of 3cm before transplanting, covering the surface of the soil with pine hair for water retention after burying to prevent the soil from becoming tight due to watering, and building a shading net.
In the step S9, after stems wither in 10 months every year in the growth process of the paris polyphylla, an organic fertilizer with the thickness of 5cm is scattered on the surface of soil, the organic fertilizer comprises 35% of cow dung, 25% of sheep dung, 15% of plant ash and 25% of straws, the organic fertilizer is prepared by stirring the components and fermenting at normal temperature for half a year, the paris polyphylla is fertilized when growing, the organic fertilizer utilizes mountain cow dung, sheep dung, plant ash and straw resources, the purposes of green, economy and environmental protection are achieved, and the organic fertilizer is fermented for a long time, so that the absorption rate of the organic fertilizer by the paris polyphylla is improved, and the growth of the paris polyphylla.
And step S9, fertilizing, weeding and pest control are carried out in the growth process of the paris polyphylla, wherein weeding is carried out before weeds grow to three times of leaves, and the planting area is uniformly disinfected by lime.
And in the step S1, selecting the wild rhizoma paridis tuber in the weather with the air temperature less than 10 ℃ in winter.
In the step S4, the rootstock is transversely cut into pieces 4cm below the terminal bud as seedlings with the terminal bud, and the pieces are cut into block structures 4cm in length.
According to the method, wild rhizoma paridis tubers are selected in winter, anti-freezing wild tubers are selected, meanwhile, screening and transplanting are carried out through seedling cultivation management for 2 years, screening and cultivation for 2-3 years are carried out after transplanting, rhizoma paridis is screened again, and finally obtained rhizoma paridis seeds are all fine seeds with high stress resistance screened layer by layer. Soaking treatment is carried out on the paris polyphylla tubers through carbendazim, the carbendazim effectively improves the antibacterial effect of the tubers, reduces the infection risk of fungi, and simultaneously increases the contact effect of the tubers and the carbendazim by a mode of rubbing the surfaces of the paris polyphylla tubers through fine sand, so that the tubers have a better antibacterial effect, and the survival rate of cultivation is increased. The method has the advantages that the elongation of cells can be accelerated by soaking the tuber of the paris polyphylla in gibberellin, the division of the cells is promoted, the expansion of the cells is promoted, the surface of the tuber of the paris polyphylla is broken through fine sand, the cells in the tuber are promoted to be soaked in the gibberellin, the germination of the tuber is promoted, the germination time of the tuber of the wild paris polyphylla is shortened, and the germination rate of the wild paris polyphylla is improved.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (9)
1. A large-scale planting method for breeding improved varieties with high stress resistance by utilizing the breeding of wild paris polyphylla tubers is characterized in that: the method comprises the following steps:
s1, tuber selection: selecting strong-growing wild rhizoma paridis tubers with a growth period of more than 4 years and no plant diseases and insect pests as seedling seeds;
s2, tuber disease-resistant treatment: mixing the tuber of the paris polyphylla with clean fine sand, slightly scraping the outer skin, generating a plurality of spot-shaped skin-broken parts on the surface of the tuber, fishing out, cleaning, and soaking in 1000 times of 50 percent carbendazim solution for 2-3 minutes;
s3, tuber growth treatment: fishing out the tubers in the step S2, and soaking the tubers in gibberellin solution with the mass percentage concentration of 0.5-0.8% for 6-10 hours at the temperature of 20-25 ℃;
s4, tuber dicing treatment: cutting the tubers into blocks;
s5, disinfection: covering quicklime on the cut wound and the scratched outer skin of the cut paris polyphylla for disinfection;
s6, seedling culture: building a sun-shading greenhouse, and transplanting tubers into the sun-shading greenhouse;
s7, growth management: keeping the soil moisture in the greenhouse to be more than 80% from the beginning of seedling culture until the end of seedling culture;
s8, transplanting: transplanting the rooted and germinated tubers into a field for culture after 2 years;
s9, growth and harvesting of paris polyphylla: and (3) producing the paris polyphylla and harvesting seeds in autumn after transplanting for 2-3 years to obtain paris polyphylla seeds with high stress resistance and good seeds.
2. The method for large-scale planting of high stress resistance improved variety by utilizing the wild paris polyphylla tuber selective breeding according to claim 1, which is characterized in that: and S6, controlling the shading degree of the greenhouse to be 50-70%, transversely ditching on the planting ground, planting according to the row spacing of 15-20cm and the plant spacing of 15-20cm during planting, transplanting the seedlings into the sun-shading greenhouse, applying base fertilizer to the seedlings for 3cm in thickness, covering soil to the seedlings for 6cm in thickness, applying surface fertilizer to the seedlings for 3cm in thickness, and covering the soil surface with rice straws, wheat straws or pine hair.
3. The method for large-scale planting of high stress resistance improved variety by utilizing the wild paris polyphylla tuber selective breeding according to claim 1, which is characterized in that: and S6, cutting off the leaves of the paris polyphylla at a position 3-5cm below the first round leaves of the seedlings after the leaves of the paris polyphylla are completely differentiated in shape.
4. The method for large-scale planting of high stress resistance improved variety by utilizing the wild paris polyphylla tuber selective breeding according to claim 1, which is characterized in that: and step S8, planting according to the row spacing of 25-30cm and the plant spacing of 15-20cm during transplanting, applying base fertilizer of 3cm before transplanting, covering the surface of the soil with pine hair for preserving water after burying to prevent the soil from becoming tight due to watering, and building a shading net.
5. The method for large-scale planting of high stress resistance improved variety by utilizing the wild paris polyphylla tuber selective breeding according to claim 1, which is characterized in that: in step S9, organic fertilizer with the thickness of 5cm is scattered on the soil surface after the stalks wither every 10 months in the growth process of the paris polyphylla.
6. The method for large-scale planting of high stress resistance improved variety by utilizing the wild paris polyphylla tuber selective breeding according to claim 5, which is characterized in that: the organic fertilizer comprises 35% of cow dung, 25% of sheep dung, 15% of plant ash and 25% of straws by weight percent, and is prepared by stirring the components and fermenting at normal temperature for half a year.
7. The method for large-scale planting of high stress resistance improved variety by utilizing the wild paris polyphylla tuber selective breeding according to claim 1, which is characterized in that: and step S9, fertilizing, weeding and pest control are carried out in the growth process of the paris polyphylla, wherein weeding is carried out before weeds grow to three times of leaves, and the planting area is uniformly disinfected by lime.
8. The method for large-scale planting of high stress resistance improved variety by utilizing the wild paris polyphylla tuber selective breeding according to claim 1, which is characterized in that: and in the step S1, selecting the wild rhizoma paridis tuber in the weather with the air temperature less than 10 ℃ in winter.
9. The method for large-scale planting of high stress resistance improved variety by utilizing the wild paris polyphylla tuber selective breeding according to claim 1, which is characterized in that: in the step S4, the rootstock is transversely cut into pieces 4cm below the terminal bud as seedlings with the terminal bud, and the pieces are cut into block structures 4cm in length.
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