AU2011285531B2 - Simplified basic media for human pluripotent cell culture - Google Patents

Simplified basic media for human pluripotent cell culture Download PDF

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AU2011285531B2
AU2011285531B2 AU2011285531A AU2011285531A AU2011285531B2 AU 2011285531 B2 AU2011285531 B2 AU 2011285531B2 AU 2011285531 A AU2011285531 A AU 2011285531A AU 2011285531 A AU2011285531 A AU 2011285531A AU 2011285531 B2 AU2011285531 B2 AU 2011285531B2
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cells
pluripotent stem
cell
medium
tgf
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AU2011285531A1 (en
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Guokai Chen
James A. Thomson
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Wisconsin Alumni Research Foundation
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/0603—Embryonic cells ; Embryoid bodies
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92—Medium free of human- or animal-derived components
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    • C12N2500/98—Xeno-free medium and culture conditions
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere

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  • Biomedical Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2011285531A 2010-08-05 2011-08-05 Simplified basic media for human pluripotent cell culture Active AU2011285531B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US37112810P 2010-08-05 2010-08-05
US61/371,128 2010-08-05
PCT/US2011/046796 WO2012019122A2 (en) 2010-08-05 2011-08-05 Simplified basic media for human pluripotent cell culture

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AU2011285531A1 AU2011285531A1 (en) 2013-02-21
AU2011285531B2 true AU2011285531B2 (en) 2015-04-30

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US (1) US9279103B2 (https=)
EP (1) EP2601288B1 (https=)
JP (2) JP6043999B2 (https=)
KR (1) KR101829488B1 (https=)
CN (2) CN108531444A (https=)
AU (1) AU2011285531B2 (https=)
BR (2) BR112013002811A8 (https=)
CA (1) CA2807418C (https=)
DK (1) DK2601288T3 (https=)
IL (1) IL224431A (https=)
SG (1) SG187699A1 (https=)
WO (1) WO2012019122A2 (https=)

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WO2012019122A3 (en) 2012-05-03
KR101829488B1 (ko) 2018-02-14
JP2017018137A (ja) 2017-01-26
CA2807418A1 (en) 2012-02-09
JP6043999B2 (ja) 2016-12-14
AU2011285531A1 (en) 2013-02-21
SG187699A1 (en) 2013-03-28
KR20130099927A (ko) 2013-09-06
BR122020000909B1 (pt) 2021-02-23
IL224431A (en) 2017-10-31
DK2601288T3 (en) 2016-05-30
CN108531444A (zh) 2018-09-14
BR112013002811A8 (pt) 2020-01-28
EP2601288A2 (en) 2013-06-12
WO2012019122A2 (en) 2012-02-09
EP2601288B1 (en) 2016-04-06
US20120202291A1 (en) 2012-08-09
BR112013002811A2 (pt) 2016-08-02
US9279103B2 (en) 2016-03-08
JP2013532492A (ja) 2013-08-19
CA2807418C (en) 2017-04-04
CN103180434A (zh) 2013-06-26

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