BR112013002811A2 - meios básicos simplificados para cultura celular pluripotente de humano - Google Patents

meios básicos simplificados para cultura celular pluripotente de humano

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Publication number
BR112013002811A2
BR112013002811A2 BR112013002811A BR112013002811A BR112013002811A2 BR 112013002811 A2 BR112013002811 A2 BR 112013002811A2 BR 112013002811 A BR112013002811 A BR 112013002811A BR 112013002811 A BR112013002811 A BR 112013002811A BR 112013002811 A2 BR112013002811 A2 BR 112013002811A2
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Brazil
Prior art keywords
cell culture
pluripotent cell
media
basic media
simplified basic
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BR112013002811A
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English (en)
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BR112013002811A8 (pt
Inventor
Guokai Chen
James A Thomson
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Wisconsin Alummi Res Foundation
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Publication date
Application filed by Wisconsin Alummi Res Foundation filed Critical Wisconsin Alummi Res Foundation
Priority to BR122020000909-8A priority Critical patent/BR122020000909B1/pt
Publication of BR112013002811A2 publication Critical patent/BR112013002811A2/pt
Publication of BR112013002811A8 publication Critical patent/BR112013002811A8/pt

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere

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  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

meios básicos simplificados para cultura celular pluripotente de humano. são descritos meios completamente definidos que suportam viabilidade, proliferação, clonagem, e derivação da célula pluripotente, bem como métodos e composições incluindo esses meios. são também escriots métodos para derivar células ips de indivíduos adultos em condições sem xeno definidas.
BR112013002811A 2010-08-05 2011-08-05 meios básicos simplificados para cultura celular pluripotente de humano BR112013002811A8 (pt)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BR122020000909-8A BR122020000909B1 (pt) 2010-08-05 2011-08-05 Método para derivar uma célula tronco pluripotente induzida (ips)humana sob condições definidas

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37112810P 2010-08-05 2010-08-05
PCT/US2011/046796 WO2012019122A2 (en) 2010-08-05 2011-08-05 Simplified basic media for human pluripotent cell culture

Publications (2)

Publication Number Publication Date
BR112013002811A2 true BR112013002811A2 (pt) 2016-08-02
BR112013002811A8 BR112013002811A8 (pt) 2020-01-28

Family

ID=44543813

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112013002811A BR112013002811A8 (pt) 2010-08-05 2011-08-05 meios básicos simplificados para cultura celular pluripotente de humano
BR122020000909-8A BR122020000909B1 (pt) 2010-08-05 2011-08-05 Método para derivar uma célula tronco pluripotente induzida (ips)humana sob condições definidas

Family Applications After (1)

Application Number Title Priority Date Filing Date
BR122020000909-8A BR122020000909B1 (pt) 2010-08-05 2011-08-05 Método para derivar uma célula tronco pluripotente induzida (ips)humana sob condições definidas

Country Status (12)

Country Link
US (1) US9279103B2 (pt)
EP (1) EP2601288B1 (pt)
JP (2) JP6043999B2 (pt)
KR (1) KR101829488B1 (pt)
CN (2) CN103180434A (pt)
AU (1) AU2011285531B2 (pt)
BR (2) BR112013002811A8 (pt)
CA (1) CA2807418C (pt)
DK (1) DK2601288T3 (pt)
IL (1) IL224431A (pt)
SG (1) SG187699A1 (pt)
WO (1) WO2012019122A2 (pt)

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CN103180434A (zh) 2013-06-26
SG187699A1 (en) 2013-03-28
EP2601288B1 (en) 2016-04-06
EP2601288A2 (en) 2013-06-12
KR20130099927A (ko) 2013-09-06
AU2011285531A1 (en) 2013-02-21
CN108531444A (zh) 2018-09-14
DK2601288T3 (en) 2016-05-30
CA2807418C (en) 2017-04-04
IL224431A (en) 2017-10-31
BR112013002811A8 (pt) 2020-01-28
US20120202291A1 (en) 2012-08-09
CA2807418A1 (en) 2012-02-09
BR122020000909B1 (pt) 2021-02-23
KR101829488B1 (ko) 2018-02-14
WO2012019122A3 (en) 2012-05-03
WO2012019122A2 (en) 2012-02-09
AU2011285531B2 (en) 2015-04-30
JP2013532492A (ja) 2013-08-19
US9279103B2 (en) 2016-03-08
JP2017018137A (ja) 2017-01-26
JP6043999B2 (ja) 2016-12-14

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