MX2016016142A - Metodos de cultivo por perfusion y usos de los mismos. - Google Patents

Metodos de cultivo por perfusion y usos de los mismos.

Info

Publication number
MX2016016142A
MX2016016142A MX2016016142A MX2016016142A MX2016016142A MX 2016016142 A MX2016016142 A MX 2016016142A MX 2016016142 A MX2016016142 A MX 2016016142A MX 2016016142 A MX2016016142 A MX 2016016142A MX 2016016142 A MX2016016142 A MX 2016016142A
Authority
MX
Mexico
Prior art keywords
methods
culturing methods
perfusion culturing
culturing
perfusion
Prior art date
Application number
MX2016016142A
Other languages
English (en)
Inventor
Yang Yang
Yang Jianguo
Villiger-Oberbek Agata
Lornzo Gabrielle
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of MX2016016142A publication Critical patent/MX2016016142A/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/26Inoculator or sampler
    • C12M1/32Inoculator or sampler multiple field or continuous type
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/12Well or multiwell plates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10Rotating vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10Perfusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

En la presente descripción se proporcionan métodos para cultivar una célula de mamífero y varios métodos que utilizan estos métodos de cultivo. Asimismo se proporcionan placas de cultivo celular de múltiples pocillos, por ejemplo, para uso en métodos de cultivo por perfusión.
MX2016016142A 2014-06-06 2015-06-05 Metodos de cultivo por perfusion y usos de los mismos. MX2016016142A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462009058P 2014-06-06 2014-06-06
PCT/US2015/034494 WO2015188106A2 (en) 2014-06-06 2015-06-05 Perfusion culturing methods and uses thereof

Publications (1)

Publication Number Publication Date
MX2016016142A true MX2016016142A (es) 2017-03-28

Family

ID=53484156

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2016016142A MX2016016142A (es) 2014-06-06 2015-06-05 Metodos de cultivo por perfusion y usos de los mismos.
MX2020009670A MX2020009670A (es) 2014-06-06 2016-12-06 Metodos de cultivo por perfusion y usos de los mismos.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2020009670A MX2020009670A (es) 2014-06-06 2016-12-06 Metodos de cultivo por perfusion y usos de los mismos.

Country Status (14)

Country Link
US (1) US11060058B2 (es)
EP (1) EP3152293A2 (es)
JP (3) JP6915992B2 (es)
KR (2) KR102479281B1 (es)
CN (1) CN106795495A (es)
AU (2) AU2015269184B2 (es)
BR (1) BR112016028512A8 (es)
CA (1) CA2951255A1 (es)
IL (1) IL249384A0 (es)
MX (2) MX2016016142A (es)
RU (3) RU2733539C2 (es)
SG (2) SG11201610167WA (es)
TW (2) TW202204596A (es)
WO (1) WO2015188106A2 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2717927T3 (es) 2013-02-22 2019-06-26 Genzyme Corp Procedimientos de cultivo por perfusión de microportadores y usos de los mismos
CA2901950A1 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
TW202204596A (zh) 2014-06-06 2022-02-01 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
BR112017013423B1 (pt) 2014-12-22 2023-04-11 Genzyme Corporation Métodos para cultivo de célula de mamífero
US11292999B2 (en) 2016-08-30 2022-04-05 Finesse Solutions, Inc. Bioreactor with multiple coupled vessels
WO2022173964A1 (en) * 2021-02-10 2022-08-18 Koniku Inc. Assay system, methods, and multi-well plate for gas stimulation of biological cells, proteins or materials
EP4323500A1 (en) * 2021-04-14 2024-02-21 Genzyme Corporation Methods of perfusion culturing a mammalian cell

Family Cites Families (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4522811A (en) 1982-07-08 1985-06-11 Syntex (U.S.A.) Inc. Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides
RU2058992C1 (ru) 1993-07-14 1996-04-27 Антипин Георгий Васильевич Способ получения кормового белка и устройство для его осуществления
DE19612966B4 (de) 1996-04-01 2009-12-10 Novartis Vaccines And Diagnostics Gmbh & Co. Kg MDCK-Zellen und Verfahren zur Vermehrung von Influenzaviren
RU2215748C2 (ru) 1999-04-09 2003-11-10 Сентро Де Инмунология Молекулар Способ получения рекомбинантного эритропоэтина человека и полученный эритропоэтин, фармацевтическая композиция
DE60043273D1 (de) 1999-04-15 2009-12-17 Crucell Holland Bv Verwendung von Rekombinanten Proteinen in Menschlichen Zellen
AT409379B (de) * 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
UA74557C2 (en) 1999-09-03 2006-01-16 Applied Research Systems A method for producing a heterologous secreted protein from chinese hamster ovaries cells grown on microcarriers
JP4176472B2 (ja) 2000-12-20 2008-11-05 バイエル・フアーマシユーチカルズ・コーポレーシヨン 哺乳動物細胞の種連続増殖(seed−trainexpansion)のための装置及び方法
PT1451290E (pt) 2001-08-31 2011-03-09 Bayer Schering Pharma Ag Uma unidade e um processo para levar a cabo a fermentação com elevada densidade celular
CN1309825C (zh) 2001-10-02 2007-04-11 诺和诺德医疗保健公司 在真核细胞中生产重组蛋白的方法
AU2002348151A1 (en) 2001-11-05 2003-05-19 Genvec, Inc. Viral vector production methods and compositions
US20030091976A1 (en) * 2001-11-14 2003-05-15 Ciphergen Biosystems, Inc. Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry
US6953692B2 (en) 2001-12-18 2005-10-11 Bayer Pharmaceuticals Corporation Device and method for seed-train expansion of mammalian cells
US20050019914A1 (en) 2003-07-24 2005-01-27 Aventis Pharma Deutschland Gmbh Perfusion process for producing erythropoietin
AR046510A1 (es) 2003-07-25 2005-12-14 Regeneron Pharma Composicion de un antagonista de vegf y un agente anti-proliferativo
EP1508576A1 (en) 2003-08-20 2005-02-23 Crucell Holland B.V. Efficient production of IgA in recombinant mammalian cells
US20050186669A1 (en) 2004-02-20 2005-08-25 Cesco Bioengineering Co., Ltd. Apparatus and method for preparing and culturing cells
EP1773978B1 (en) 2004-05-19 2014-04-02 Massachusetts Institute Of Technology Perfused three-dimensional cell/tissue disease models
EP1789528A4 (en) 2004-09-16 2011-07-20 Becton Dickinson Co BIOREACTOR FOR CULTIVATION OF CELLS UNDER PERFUSION
US7767446B2 (en) * 2004-09-16 2010-08-03 Becton, Dickinson And Company Perfusion bioreactors for culturing cells
PT2550971T (pt) 2004-09-30 2017-10-02 Bayer Healthcare Llc Dispositivos e métodos para fabrico contínuo integrado de moléculas biológicas
ES2662589T3 (es) 2005-06-15 2018-04-09 Zhejiang Jinyishengshi Bioengineering Co., Ltd. Recipientes para cultivo en suspensión
WO2006138143A1 (en) 2005-06-15 2006-12-28 Amprotein Corporation Suspension culture vessels
WO2008024769A2 (en) 2006-08-21 2008-02-28 Raven Biotechnologies, Inc. Intensified perfusion production method
WO2008073620A2 (en) 2006-11-02 2008-06-19 Neose Technologies, Inc. Manufacturing process for the production of polypeptides expressed in insect cell-lines
EP2118270A2 (en) * 2007-01-08 2009-11-18 Millipore Corporation Cell culture methods for producing recombinant proteins in the presence of reduced levels of one or more contaminants
WO2008106515A1 (en) * 2007-02-27 2008-09-04 The Board Of Trustees Of The University Of Illinois Insert device for multiwell plate
EP2126093B1 (en) 2007-03-02 2012-10-10 Boehringer Ingelheim Pharma GmbH & Co. KG Improvement of protein production
US9012212B2 (en) 2007-04-17 2015-04-21 Xendo Holding B.V. Method and device for continuous membrane adsorption
TW200902708A (en) 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
US9109193B2 (en) 2007-07-30 2015-08-18 Ge Healthcare Bio-Sciences Corp. Continuous perfusion bioreactor system
EP3327132A3 (en) 2007-08-09 2018-07-18 Wyeth LLC Use of perfusion to enhance production of fed-batch cell culture in bioreactors
WO2009034186A2 (en) 2007-09-13 2009-03-19 Helmholtz-Zentrum für Infektionsforschung GmbH Process for cell cultivation
US20090233334A1 (en) 2008-03-11 2009-09-17 Excellgene Sa Cell cultivation and production of recombinant proteins by means of an orbital shake bioreactor system with disposable bags at the 1,500 liter scale
DE102008019691A1 (de) * 2008-04-15 2009-10-29 Technische Universität Ilmenau Teilaktives mikrofluidisches System für die 3D-Zellkultivierung sowie Verfahren zu dessen Perfusion
WO2010005746A1 (en) 2008-06-16 2010-01-14 Amprotein Corporation Bioreactors
JP2012506257A (ja) * 2008-10-22 2012-03-15 バイオベスト インターナショナル インコーポレイテッド 細胞および細胞由来生成物の産生のための、灌流バイオリアクター、細胞培養システム、および方法
US8617879B2 (en) * 2009-03-26 2013-12-31 Agency For Science, Technology And Research Apparatus for cell or tissue culture
WO2010120514A2 (en) 2009-03-31 2010-10-21 The Trustees Of The University Of Pennsylvania Antigen-binding proteins comprising recombinant protein scaffolds
CN101875917B (zh) 2009-04-28 2012-07-04 北京清大天一科技有限公司 生物反应器微载体培养动物细胞生产猪瘟疫苗的方法
US10119112B2 (en) 2010-03-02 2018-11-06 Universite Technologie de Compiegne—UTC Multi-reactor unit for dynamic cell culture
CN201933090U (zh) 2010-11-22 2011-08-17 朱学仁 细胞微载体灌注培养截留装置
KR102027596B1 (ko) 2010-12-06 2019-10-01 타폰 바이오시스템즈, 인코포레이티드 생물학적 산물을 위한 연속 처리 방법
EP2665806B1 (en) 2011-01-17 2021-07-28 F. Hoffmann-La Roche AG Separation apparatus
KR101741650B1 (ko) 2011-03-22 2017-05-31 가부시키가이샤 도카이히트 현미경 관찰용 배양장치 및 그 사용 방법
EP2707488B1 (en) 2011-05-12 2017-11-15 ProBioGen AG Enhancement of protein production yield mediated by a fast shuttling cdc42 gtpase
PL2726600T3 (pl) 2011-07-01 2017-08-31 Amgen Inc. Hodowla ssaczych komórek
WO2013116449A1 (en) * 2012-02-02 2013-08-08 Corning Incorporated Automatic continuous perfusion cell culture microplate consumables
JP2015517804A (ja) 2012-04-01 2015-06-25 イー・エム・デイー・ミリポア・コーポレイシヨン 細胞培養及び傾斜移動アッセイ方法及び装置
EP3747984A1 (en) 2012-10-23 2020-12-09 Genzyme Corporation Perfusion culturing methods and uses thereof
CA2901950A1 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
ES2717927T3 (es) 2013-02-22 2019-06-26 Genzyme Corp Procedimientos de cultivo por perfusión de microportadores y usos de los mismos
SG11201506775VA (en) 2013-03-08 2015-09-29 Genzyme Corp Continuous purification of therapeutic proteins
JP6742902B2 (ja) 2013-03-15 2020-08-19 ジェンザイム・コーポレーション 高密度細胞バンキングの方法
US9481901B2 (en) * 2013-05-30 2016-11-01 Amgen Inc. Methods for increasing mannose content of recombinant proteins
WO2015039115A1 (en) 2013-09-16 2015-03-19 Genzyme Corporation Methods and systems for processing a cell culture
US20160289633A1 (en) 2013-12-20 2016-10-06 Biogen Ma Inc. Use of Perfusion Seed Cultures to Improve Biopharmaceutical Fed-Batch Production Capacity and Product Quality
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
TWI709570B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析法及製法
SG10202113019XA (en) 2014-06-04 2021-12-30 Amgen Inc Methods for harvesting mammalian cell cultures
TW202204596A (zh) * 2014-06-06 2022-02-01 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
CN104480897B (zh) 2014-11-28 2017-01-04 上海市政工程设计研究总院(集团)有限公司 陆域占地少应用范围广的防汛墙
CN104450597B (zh) 2014-12-10 2020-07-28 青岛农业大学 一种石油降解菌固体菌剂的制备方法及由其制备的固体菌剂修复石油污染的土壤的方法
BR112017013423B1 (pt) 2014-12-22 2023-04-11 Genzyme Corporation Métodos para cultivo de célula de mamífero

Also Published As

Publication number Publication date
JP2021168681A (ja) 2021-10-28
EP3152293A2 (en) 2017-04-12
WO2015188106A3 (en) 2016-01-28
RU2708919C2 (ru) 2019-12-12
AU2015269184A1 (en) 2016-12-22
RU2016152238A (ru) 2018-07-10
CN106795495A (zh) 2017-05-31
BR112016028512A2 (pt) 2017-08-22
TW202204596A (zh) 2022-02-01
CA2951255A1 (en) 2015-12-10
TW201614062A (en) 2016-04-16
WO2015188106A2 (en) 2015-12-10
US20160017280A1 (en) 2016-01-21
MX2020009670A (es) 2020-10-08
JP2017520242A (ja) 2017-07-27
RU2019138327A3 (es) 2020-04-02
RU2733539C2 (ru) 2020-10-05
AU2021205004A1 (en) 2021-09-23
RU2020131199A3 (es) 2022-03-22
KR20170010892A (ko) 2017-02-01
TWI743024B (zh) 2021-10-21
SG11201610167WA (en) 2017-01-27
JP2023120382A (ja) 2023-08-29
BR112016028512A8 (pt) 2021-03-16
AU2015269184B2 (en) 2021-04-15
JP6915992B2 (ja) 2021-08-11
KR102479281B1 (ko) 2022-12-20
RU2019138327A (ru) 2019-12-17
KR102611855B1 (ko) 2023-12-07
US20210324328A1 (en) 2021-10-21
IL249384A0 (en) 2017-02-28
KR20230003634A (ko) 2023-01-06
US11060058B2 (en) 2021-07-13
JP7350810B2 (ja) 2023-09-26
SG10202008131WA (en) 2020-10-29
RU2016152238A3 (es) 2019-05-30
RU2020131199A (ru) 2022-03-22

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