ATE332398T1 - Einfach markierte oligonukleotidsonden - Google Patents

Einfach markierte oligonukleotidsonden

Info

Publication number
ATE332398T1
ATE332398T1 AT01959722T AT01959722T ATE332398T1 AT E332398 T1 ATE332398 T1 AT E332398T1 AT 01959722 T AT01959722 T AT 01959722T AT 01959722 T AT01959722 T AT 01959722T AT E332398 T1 ATE332398 T1 AT E332398T1
Authority
AT
Austria
Prior art keywords
probes
nucleic acid
methods
labeled oligonucleotide
oligonucleotide probe
Prior art date
Application number
AT01959722T
Other languages
German (de)
English (en)
Inventor
Carl T Wittwer
Andrew O Crockett
Brian E Caplin
Wade Stevenson
Lori A Wagner
Jian Chen
Noriko Kusukawa
Original Assignee
Univ Utah Res Found
Idaho Technology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Utah Res Found, Idaho Technology Inc filed Critical Univ Utah Res Found
Application granted granted Critical
Publication of ATE332398T1 publication Critical patent/ATE332398T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
AT01959722T 2000-08-11 2001-08-10 Einfach markierte oligonukleotidsonden ATE332398T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22472600P 2000-08-11 2000-08-11
US24061000P 2000-10-16 2000-10-16

Publications (1)

Publication Number Publication Date
ATE332398T1 true ATE332398T1 (de) 2006-07-15

Family

ID=26918967

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01959722T ATE332398T1 (de) 2000-08-11 2001-08-10 Einfach markierte oligonukleotidsonden

Country Status (8)

Country Link
US (1) US6635427B2 (cg-RX-API-DMAC7.html)
EP (1) EP1307592B1 (cg-RX-API-DMAC7.html)
JP (1) JP5213297B2 (cg-RX-API-DMAC7.html)
AT (1) ATE332398T1 (cg-RX-API-DMAC7.html)
CA (1) CA2417986C (cg-RX-API-DMAC7.html)
DE (1) DE60121342T2 (cg-RX-API-DMAC7.html)
ES (1) ES2267803T3 (cg-RX-API-DMAC7.html)
WO (1) WO2002014555A2 (cg-RX-API-DMAC7.html)

Families Citing this family (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
FI20000333A0 (fi) * 2000-02-16 2000-02-16 Jussi Nurmi Homogeeninen menetelmä polynukleotidin havaitsemiseksi
ES2269365T3 (es) * 2000-03-29 2007-04-01 Lgc Limited Baliza de hibridacion y metodo de deteccion y discriminacion rapidas de secuencias.
US7998673B2 (en) * 2000-03-29 2011-08-16 Lgc Limited Hybridisation beacon and method of rapid sequence detection and discrimination
US8409508B2 (en) 2002-04-23 2013-04-02 Biofire Diagnostics, Inc. Sample withdrawal and dispensing device
US7785776B2 (en) * 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
CA2433473A1 (en) * 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
US7122384B2 (en) * 2002-11-06 2006-10-17 E. I. Du Pont De Nemours And Company Resonant light scattering microparticle methods
WO2004053155A1 (en) 2002-12-06 2004-06-24 Roche Diagniostics Gmbh Multiplex assay detection of pathogenic organisms
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
EP1613771B1 (en) 2003-04-04 2012-03-21 Roche Diagnostics GmbH Improved system for multi color real time pcr
JP4454249B2 (ja) * 2003-04-18 2010-04-21 アークレイ株式会社 膵ラ氏島アミロイドタンパク質変異遺伝子の検出法ならびにそのための核酸プローブおよびキット
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
WO2005051967A2 (en) * 2003-11-19 2005-06-09 Allelogic Biosciences Corp. Oligonucleotides labeled with a plurality of fluorophores
FR2866349B1 (fr) * 2004-02-12 2008-05-16 Biomerieux Sa Procede de diagnostic/pronostic d'une thrombose
WO2005098036A1 (en) * 2004-04-12 2005-10-20 Postech Foundation Oligonucleotide for detecting target dna or rna
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
CA2594223C (en) * 2005-01-03 2016-03-15 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
ATE359500T1 (de) 2005-01-18 2007-05-15 Hoffmann La Roche Fluoreszenzabbildung mittels telezentrizität
EP1681555B1 (en) 2005-01-18 2007-03-21 Roche Diagnostics GmbH Imaging of fluorescence signals using telecentric excitation and imaging optics
US20060172324A1 (en) * 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature
JP4398886B2 (ja) * 2005-03-07 2010-01-13 ソニー株式会社 通信端末装置、通信システム、通信方法、およびプログラム
US7759469B2 (en) 2005-03-10 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
EP1700922B1 (en) 2005-03-10 2016-08-24 Roche Diagnostics GmbH 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them
US7776567B2 (en) * 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
ATE443072T1 (de) 2005-11-23 2009-10-15 Roche Diagnostics Gmbh Polynukleotid mit phosphatmimetikum
US7759470B2 (en) * 2006-02-20 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
EP1989324B1 (en) 2006-02-27 2011-05-18 Roche Diagnostics GmbH Pcr hot start by magnesium sequestration
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
WO2008020090A1 (es) * 2006-08-18 2008-02-21 Laboratorios Indas, S.A. Método y kit para diagnosticar el riesgo de desarrollar una enfermedad trombótica venosa
KR101433208B1 (ko) 2006-12-13 2014-08-22 루미넥스 코포레이션 실시간으로 pcr의 다중 분석을 위한 시스템과 방법
EP2126114B1 (en) * 2007-02-01 2016-09-28 Abacus Diagnostica OY Method for detection of presence of target polynucleotide in samples
CN101622363B (zh) * 2007-07-13 2012-09-05 爱科来株式会社 用于检测jak2基因的突变的探针及其用途
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
AU2009262870B2 (en) 2008-05-30 2014-03-20 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Salmonella
US9393566B2 (en) * 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
EP2148187A1 (de) 2008-07-25 2010-01-27 Roche Diagnostics GmbH Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion
WO2010018245A1 (es) * 2008-08-12 2010-02-18 Biotools Biotechnological & Medical Laboratories, S.A. Método para la detección y/o cuantificación de un ácido nucleico substrato
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ.ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
CN102449167B (zh) 2009-05-26 2014-12-17 厦门大学 一种检测核酸序列变异的方法
WO2010147848A2 (en) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
CN102597271B (zh) * 2009-10-30 2015-05-20 爱科来株式会社 Egfr基因多态性检测用探针及其用途
EP2502994A4 (en) * 2009-11-19 2013-05-22 Arkray Inc PRIMER SET FOR MTHFR GENE AMPLIFICATION, MTHFR GENE AMPLIFICATION REAGENT COMPRISING THE SAME, AND USE THEREOF
JP5917144B2 (ja) * 2009-12-07 2016-05-11 アークレイ株式会社 疾患関連遺伝子の多型検出用プローブおよびその用途
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
EP2562255A4 (en) * 2010-04-21 2013-10-30 Riken NUCLEIC ACID BASIC ANALOG WITH EXTINGUISHING PROPERTIES AND FLUORESCENCE AND APPLICATION THEREOF
JP5940771B2 (ja) 2010-07-12 2016-06-29 アークレイ株式会社 abl遺伝子多型の検出用プローブおよびその用途
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
JP2012235778A (ja) * 2011-04-28 2012-12-06 Arkray Inc アセトアルデヒド脱水素酵素2及びアルコール脱水素酵素2の遺伝子変異を同時に検出する方法
JP6144623B2 (ja) * 2011-06-16 2017-06-07 日鉄住金環境株式会社 核酸測定用の核酸プローブ
US10557170B2 (en) 2011-06-16 2020-02-11 Gendiag.Exe, S.L. Thromboembolic disease markers
PT2535424E (pt) * 2011-06-16 2015-10-29 Gendiag Exe Sl Snps associados a doença tromboembólica
EP2753927B1 (en) * 2011-09-06 2017-11-15 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Methods for analyzing biological macromolecular complexes and use thereof
JP2013074888A (ja) * 2011-09-15 2013-04-25 Arkray Inc IL28B(rs8099917)とITPA(rs1127354)の変異を検出する方法
WO2013112458A1 (en) 2012-01-24 2013-08-01 Beth Israel Deaconess Medical Center, Inc. Novel chrebp isoforms and methods using the same
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
JP2016509480A (ja) 2013-01-24 2016-03-31 カリフォルニア インスティチュート オブ テクノロジー 発色団に基づく特徴づけおよび検出方法
CA2912312C (en) 2013-05-20 2023-01-24 Board Of Trustees Of The University Of Arkansas Gep5 model for multiple myeloma
GB201319180D0 (en) * 2013-10-30 2013-12-11 Mast Group Ltd Nucleic acid probe
WO2015200855A1 (en) 2014-06-26 2015-12-30 University Of Florida Research Foundation, Inc. Rapid detection of infectious agents
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
ES2961374T3 (es) 2015-04-24 2024-03-11 Atila Biosystems Incorporated Amplificación con cebadores de composición de nucleótidos limitada
EP3783108B1 (en) * 2016-02-09 2025-01-29 Eiken Kagaku Kabushiki Kaisha Method for detecting target nucleic acid
ES2926513T3 (es) 2016-07-29 2022-10-26 Juno Therapeutics Inc Métodos para evaluar la presencia o ausencia de virus competente en replicación
WO2019006427A1 (en) 2017-06-29 2019-01-03 Juno Therapeutics, Inc. WALL MODEL FOR ASSESSING TOXICITIES ASSOCIATED WITH IMMUNOTHERAPIES
KR20200064060A (ko) 2017-07-29 2020-06-05 주노 쎄러퓨티크스 인코퍼레이티드 재조합 수용체를 발현하는 세포 증폭용 시약
MA50057A (fr) 2017-09-01 2020-07-08 Juno Therapeutics Inc Expression génique et évaluation d'un risque de développement d'une toxicité suite à une thérapie cellulaire
CN107727618B (zh) * 2017-09-04 2019-12-31 江苏大学 生物芯片和特异性切割位点dna序列检测汞离子的方法
US20210254000A1 (en) 2017-11-01 2021-08-19 Juno Therapeutics, Inc. Process for producing a t cell composition
WO2019152747A1 (en) 2018-01-31 2019-08-08 Juno Therapeutics, Inc. Methods and reagents for assessing the presence or absence of replication competent virus
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
CA3117720A1 (en) 2018-11-06 2020-05-14 Juno Therapeutics, Inc. Process for producing genetically engineered t cells
BR112021020447A2 (pt) 2019-04-17 2022-05-17 Alpine Immune Sciences Inc Métodos e usos de proteínas de fusão de ligante icos variante (icosl)
CN114269371A (zh) 2019-06-12 2022-04-01 朱诺治疗学股份有限公司 细胞介导的细胞毒性疗法与促存活bcl2家族蛋白的抑制剂的组合疗法
CN114555112A (zh) 2019-08-22 2022-05-27 朱诺治疗学股份有限公司 T细胞疗法和zeste增强子同源物2(ezh2)抑制剂的组合疗法及相关方法
NZ786111A (en) * 2019-09-23 2025-10-31 Univ Gent Probe and method for str-genotyping
KR102107589B1 (ko) * 2019-11-20 2020-05-07 주식회사 유진셀 단일 신호 형광 물질을 이용한 실시간 타겟 핵산 검출 및 정량 방법
WO2021140173A1 (en) 2020-01-10 2021-07-15 Biouniversa S.R.L. Methods and uses for treating fibrotic solid tumors with bags inhibitors
EP4090734B1 (en) 2020-01-13 2024-11-06 Aspen Neuroscience, Inc. Method of differentiating neural cells and related compositions and methods of use
KR20230022868A (ko) 2020-05-13 2023-02-16 주노 쎄러퓨티크스 인코퍼레이티드 재조합 수용체를 발현하는 공여자-배치 세포의 제조 방법
WO2021260186A1 (en) 2020-06-26 2021-12-30 Juno Therapeutics Gmbh Engineered t cells conditionally expressing a recombinant receptor, related polynucleotides and methods
WO2023004371A1 (en) 2021-07-21 2023-01-26 Aspen Neuroscience, Inc. Methods of differentiating neural cells and predicting engraftment thereof and related compositions
AU2022402153A1 (en) 2021-12-03 2024-06-13 Medicinal Genomics Corporation Psilocybe assay
CN119384700A (zh) 2022-04-15 2025-01-28 艾斯本神经科学公司 细胞分化状态的分类方法和分化细胞的相关组合物
EP4532695A1 (en) 2022-05-25 2025-04-09 Celgene Corporation Methods of manufacturing t cell therapies
WO2024059493A1 (en) 2022-09-13 2024-03-21 Medicinal Genomics Corporation Psilocybe assay
WO2025245399A1 (en) 2024-05-23 2025-11-27 Indapta Therapeutics, Inc. Method of treating cancer with natural killer cells

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5597696A (en) * 1994-07-18 1997-01-28 Becton Dickinson And Company Covalent cyanine dye oligonucleotide conjugates
DE69610304T2 (de) 1995-05-05 2001-04-05 Perkin-Elmer Corp., Foster City Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
GB9602025D0 (en) * 1996-02-01 1996-04-03 Amersham Int Plc Nucleoside analogues
US5716784A (en) 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
CA2253969A1 (en) 1996-05-15 1997-11-20 Rajkumar Siva Iyer Non-nucleotide linking reagents
ATE318327T1 (de) 1996-06-04 2006-03-15 Univ Utah Res Found Fluoreszenz-donor-akzeptor paar
US5691145A (en) 1996-08-27 1997-11-25 Becton, Dickinson And Company Detection of nucleic acids using G-quartets
WO1998026093A2 (en) * 1996-12-13 1998-06-18 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization
SE522077C2 (sv) * 1997-09-05 2004-01-13 Lightup Technologies Ab Förfarande att välja sekvens hos sond för nukleinsyrahybridisering
JP2001514859A (ja) * 1997-09-04 2001-09-18 バイエル コーポレイション クエンチ可能な蛍光標識を有するオリゴヌクレオチドプローブおよびその使用方法
DE19858588B4 (de) 1998-08-22 2016-04-07 Qiagen Gmbh Farbstoffmarkiertes Oligonukleotid zum Markieren eines Nukleinsäuremoleküls
DE60045059D1 (de) 1999-04-20 2010-11-18 Nat Inst Of Advanced Ind Scien Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten
SE9902565D0 (sv) * 1999-07-05 1999-07-05 Jonas Karlsson Probe for analysis of nucleic acids
ES2269365T3 (es) 2000-03-29 2007-04-01 Lgc Limited Baliza de hibridacion y metodo de deteccion y discriminacion rapidas de secuencias.

Also Published As

Publication number Publication date
DE60121342D1 (de) 2006-08-17
US6635427B2 (en) 2003-10-21
EP1307592A2 (en) 2003-05-07
CA2417986A1 (en) 2002-02-21
WO2002014555A3 (en) 2003-02-27
DE60121342T2 (de) 2007-06-28
WO2002014555A2 (en) 2002-02-21
EP1307592B1 (en) 2006-07-05
JP5213297B2 (ja) 2013-06-19
ES2267803T3 (es) 2007-03-16
US20030022177A1 (en) 2003-01-30
CA2417986C (en) 2013-11-26
JP2004506431A (ja) 2004-03-04

Similar Documents

Publication Publication Date Title
ATE332398T1 (de) Einfach markierte oligonukleotidsonden
ATE534748T1 (de) Direkter snp-nachweis mit nichtamplifizierter dna
ATE443777T1 (de) Methoden und zusammensetzungen zur analyse von ribonukleinsäuren
WO2003008622A3 (en) Exponential nucleic acid amplification using nicking endonucleases
ATE366821T1 (de) Automatisierte echtzeit-analyse von nukleinsäureamplifikation
JP2004506431A5 (cg-RX-API-DMAC7.html)
DK1278889T3 (da) Hydridiseringsbeacon og fremgangsmåde til hurtig sekvensdetektion og distinktion
WO2003087297A8 (en) Infectious disease microarray
EP1295941A4 (en) NEW NUCLEIC ACID ESTERS AND METHOD FOR TESTING NUCLEIC ACIDS UNDER USE
DE60136567D1 (de) Polynukleotid-proben zum nachweis und zur quantifizierung von candida spezies
PT1633888E (pt) Meios e método para a detecção de sequências de nucleótidos alvo utilizando ensaios de ligação com pares de sondas de oligonucleótido melhoradas
ATE474933T1 (de) Verfahren zum nachweis von pathogenen organismen
ATE366325T1 (de) Verfahren zum nachweisen von mikroorganismen in einer probe
WO2005047468A8 (en) Improved methods for detecting and measuring specific nucleic acid sequences
DE60327345D1 (de) Nachweissystem
ATE197071T1 (de) Nachweis von nukleinsäure-amplifikation mittels fluoreszenz-polarisation
ATE445715T1 (de) Elektrokatalytischer nukleinsäurehybridisierungsnachweis
DE60330160D1 (de) Oligonukleotide zur Detektion von Hepatitis B Viren
ATE455864T1 (de) Verfahren zum nachweis von in einer polymerase- kettenreaktion amplifizierten nukleinsäuremolekülen
DE60119902D1 (de) Verfahren für die gleichzeitige und mehrfache detektion und quantifizierung der hybridisierung von molekülverbindungen wie nukleinsäuren, dns, rns, pns und proteinen
ATE383579T1 (de) Verkleinerung der fluoreszenzlöschung während einer bestimmung
CY1105861T1 (el) Μεθοδος για την ανιχνευση δευτepευοντων γονιδιωματων σε τυπους ιου χρησιμοποιωντας μικροτσιπ dna
ATE455867T1 (de) Echtzeit-pcr unter zusatz von pyrophosphatase
DE602004030430D1 (de) Verfahren zur Quantifizierung von methylierter DNS
ATE307906T1 (de) Verfahren zum nachweis von gram-positiven bakterien

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties