ATE284966T1 - Zirkuläres dna molekül mit einem abhängigen replikationsursprung, seine herstellungsverfahren und seine verwendung in der gentherapie - Google Patents

Zirkuläres dna molekül mit einem abhängigen replikationsursprung, seine herstellungsverfahren und seine verwendung in der gentherapie

Info

Publication number
ATE284966T1
ATE284966T1 AT96931124T AT96931124T ATE284966T1 AT E284966 T1 ATE284966 T1 AT E284966T1 AT 96931124 T AT96931124 T AT 96931124T AT 96931124 T AT96931124 T AT 96931124T AT E284966 T1 ATE284966 T1 AT E284966T1
Authority
AT
Austria
Prior art keywords
gene therapy
dna molecule
circular dna
production process
replication origin
Prior art date
Application number
AT96931124T
Other languages
English (en)
Inventor
Joel Crouzet
Fabienne Soubrier
Original Assignee
Gencell Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=9482580&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE284966(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gencell Sa filed Critical Gencell Sa
Application granted granted Critical
Publication of ATE284966T1 publication Critical patent/ATE284966T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/02—Muscle relaxants, e.g. for tetanus or cramps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factor [FGF]
    • C07K14/501—Fibroblast growth factor [FGF] acidic FGF [aFGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Neurosurgery (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Virology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Communicable Diseases (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
AT96931124T 1995-09-15 1996-09-13 Zirkuläres dna molekül mit einem abhängigen replikationsursprung, seine herstellungsverfahren und seine verwendung in der gentherapie ATE284966T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9510825A FR2738842B1 (fr) 1995-09-15 1995-09-15 Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique
PCT/FR1996/001414 WO1997010343A1 (fr) 1995-09-15 1996-09-13 Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique

Publications (1)

Publication Number Publication Date
ATE284966T1 true ATE284966T1 (de) 2005-01-15

Family

ID=9482580

Family Applications (3)

Application Number Title Priority Date Filing Date
AT96931124T ATE284966T1 (de) 1995-09-15 1996-09-13 Zirkuläres dna molekül mit einem abhängigen replikationsursprung, seine herstellungsverfahren und seine verwendung in der gentherapie
AT06017245T ATE492642T1 (de) 1995-09-15 1996-09-13 Zirkuläres dna molekül mit einem abhängigen replikationsursprung, seine herstellungsverfahren und seine verwendung in der gentherapie
AT04016978T ATE352630T1 (de) 1995-09-15 1996-09-13 Zirkuläres dna molekül mit einem abhängigen replikationsursprung, seine herstellungsverfahren und seine verwendung in der gentherapie

Family Applications After (2)

Application Number Title Priority Date Filing Date
AT06017245T ATE492642T1 (de) 1995-09-15 1996-09-13 Zirkuläres dna molekül mit einem abhängigen replikationsursprung, seine herstellungsverfahren und seine verwendung in der gentherapie
AT04016978T ATE352630T1 (de) 1995-09-15 1996-09-13 Zirkuläres dna molekül mit einem abhängigen replikationsursprung, seine herstellungsverfahren und seine verwendung in der gentherapie

Country Status (20)

Country Link
US (3) US6977174B2 (de)
EP (3) EP1508620B1 (de)
JP (3) JP3818665B2 (de)
KR (1) KR100522369B1 (de)
AT (3) ATE284966T1 (de)
BR (1) BRPI9610511B8 (de)
CA (1) CA2229307C (de)
CZ (1) CZ294525B6 (de)
DE (3) DE69636878T2 (de)
DK (3) DK1508620T3 (de)
ES (3) ES2357098T3 (de)
FR (1) FR2738842B1 (de)
HU (1) HU224400B1 (de)
IL (4) IL123641A0 (de)
NO (1) NO323975B1 (de)
PT (3) PT850310E (de)
SK (1) SK285317B6 (de)
TW (1) TWI231826B (de)
WO (1) WO1997010343A1 (de)
ZA (1) ZA967640B (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7038026B2 (en) * 2000-05-26 2006-05-02 Centelion Purification of a triple heli formation with an immobilized oligonucleotide
FR2738842B1 (fr) * 1995-09-15 1997-10-31 Rhone Poulenc Rorer Sa Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique
US7279313B2 (en) * 1995-09-15 2007-10-09 Centelion Circular DNA molecule having a conditional origin of replication, process for their preparation and their use in gene therapy
US6156574A (en) 1997-06-23 2000-12-05 The Rockefeller University Methods of performing gene trapping in bacterial and bacteriophage-derived artificial chromosomes and use thereof
US6821759B1 (en) 1997-06-23 2004-11-23 The Rockefeller University Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof
EP0991426B2 (de) 1997-06-30 2007-03-28 Institut Gustave Roussy Verabreichung der nukleinsäure in den quergestreiften muskel
US6022716A (en) * 1998-04-10 2000-02-08 Genset Sa High throughput DNA sequencing vector
CA2336590A1 (en) * 1999-06-07 2000-12-14 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
WO2001005962A1 (en) * 1999-07-20 2001-01-25 The Rockefeller University Conditional homologous recombination of large genomic vector inserts
FR2821855B1 (fr) † 2001-03-09 2004-04-02 Cayla Genes synthetiques et plasmides bacteriens depourvus de cpg
FR2822476B1 (fr) * 2001-03-23 2004-04-02 Aventis Pharma Sa Procedes de purification et de detection de sequences cibles d'adn double brin par interaction triple helice
WO2003004088A2 (en) 2001-07-05 2003-01-16 Aventis Pharma S.A. Method of administration of a gene of interest to the heart and vasculature
US6989261B2 (en) * 2001-12-20 2006-01-24 Eli Lilly And Company Butyrylcholinesterase variant polypeptides with increased catalytic efficiency and methods of use
US7049121B2 (en) 2001-12-20 2006-05-23 Applied Molecular Evolution Butyrylcholinesterase variant polypeptides with increased catalytic efficiency and methods of use
FI116068B (fi) 2003-09-15 2005-09-15 Fit Biotech Oyj Plc Uusi selektiojärjestelmä, siinä käyttökelpoinen vektori, bakteerisolukantoja sekä menetelmä solujen valikoimiseksi
JP4581851B2 (ja) * 2004-07-27 2010-11-17 セイコーエプソン株式会社 電気光学装置の駆動回路及び駆動方法、電気光学装置並びに電子機器
US8202727B2 (en) * 2005-05-17 2012-06-19 Ozgene Pty Ltd. Sequential cloning system
WO2007030588A1 (en) * 2005-09-07 2007-03-15 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Use of replicators to prevent gene silencing
US20090053295A1 (en) * 2005-10-01 2009-02-26 Charles Stout Regulatable fusion promoters
GB0606190D0 (en) 2006-03-28 2006-05-10 Isis Innovation Construct
JP5774480B2 (ja) * 2008-07-18 2015-09-09 マックスサイト インコーポレーティッド 電気穿孔を最適化するための方法
EP2221066A1 (de) 2009-02-18 2010-08-25 Sanofi-Aventis Verwendung des VgII3-Aktivitätsmodulators zur Modulierung von Adipogenese
CA2792696C (en) 2010-03-17 2020-01-07 Association Institut De Myologie Modified u7 snrnas for treatment of neuromuscular diseases
WO2012069657A1 (en) 2010-11-26 2012-05-31 Institut Pasteur Identification of a human gyrovirus and applications.
WO2013083753A2 (en) 2011-12-07 2013-06-13 Institut Pasteur Identification of a swine parecho-like virus and applications
US9550998B2 (en) 2012-08-29 2017-01-24 Nature Technology Corporation DNA plasmids with improved expression
US20150322439A1 (en) * 2012-11-19 2015-11-12 Nature Technology Corporation Replicative minicircle vectors with improved expression
CN105555954B (zh) * 2013-03-14 2019-03-26 吉恩维沃公司 改进的胸苷激酶基因
EP3456821B2 (de) 2017-09-19 2024-01-24 Deutsches Krebsforschungszentrum Nicht integrierende dna-vektoren zur genetischen modifikation von zellen
WO2019057774A1 (en) 2017-09-19 2019-03-28 Deutsches Krebsforschungszentrum NON-INTEGRATING DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS
SG11202009009UA (en) 2018-03-21 2020-10-29 Nature Tech Corporation Viral and non-viral nanoplasmid vectors with improved production
WO2023241567A1 (zh) * 2022-06-13 2023-12-21 南京金斯瑞生物科技有限公司 一种可提高无筛选标签质粒制备效率的野生型-突变体π蛋白切换表达系统

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4190495A (en) * 1976-09-27 1980-02-26 Research Corporation Modified microorganisms and method of preparing and using same
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
US4727028A (en) * 1981-06-22 1988-02-23 Eli Lilly And Company Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof
US4654307A (en) * 1983-02-17 1987-03-31 The Research Foundation Of State University Of New York Novel bacteria containing a plasmid having a tRNA code
US4761367A (en) * 1984-11-07 1988-08-02 The University Of North Carolina At Chapel Hill Vectors suitable for detection of eukaryotic DNA regulatory sequences
US5859208A (en) * 1988-07-06 1999-01-12 Fiddes; John C. Human basic fibroblast growth factor analog
US5198343A (en) * 1986-08-05 1993-03-30 Transgene, S.A. Method for expressing a heterologous protein in a dapD- mutant of E. coli and the strain obtained
US5591632A (en) * 1987-03-02 1997-01-07 Beth Israel Hospital Recombinant BCG
US5510099A (en) * 1987-05-01 1996-04-23 Stratagene Mutagenesis testing using transgenic non-human animals carrying test DNA sequences
US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
US5693622A (en) * 1989-03-21 1997-12-02 Vical Incorporated Expression of exogenous polynucleotide sequences cardiac muscle of a mammal
JPH03187383A (ja) * 1989-09-04 1991-08-15 Takeda Chem Ind Ltd 発現プラスミドおよびその用途
EP0485701A1 (de) * 1990-09-28 1992-05-20 American Cyanamid Company Einfügen von DNS mittels abgewandelter Transposons
TW201794B (de) * 1991-05-03 1993-03-11 American Cyanamid Co
US5714323A (en) * 1991-08-30 1998-02-03 The University Of Medecine And Dentistry Of New Jersey Over expression of single-stranded molecules
US5444149A (en) * 1992-05-11 1995-08-22 Duke University Methods and compositions useful in the recognition, binding and expression of ribonucleic acids involved in cell growth, neoplasia and immunoregulation
US5434065A (en) * 1993-05-06 1995-07-18 President And Fellows Of Harvard College In vivo selection of microbial virulence genes
AU7568094A (en) * 1993-08-12 1995-03-14 Cytotherapeutics, Inc. Improved compositions and methods for the delivery of biologically active molecules using genetically altered cells contained in biocompatible immunoisolatory capsules
US5679647A (en) * 1993-08-26 1997-10-21 The Regents Of The University Of California Methods and devices for immunizing a host against tumor-associated antigens through administration of naked polynucleotides which encode tumor-associated antigenic peptides
US5700657A (en) * 1993-12-13 1997-12-23 Genzyme Corporation Vectors and vector systems including genes encoding tumor suppressor proteins and producer cells transformed thereby
IL109558A (en) * 1994-05-04 2004-08-31 Yissum Res Dev Co DNA structures Derived from the SV40 virus that include DNA sequences External
AU2946695A (en) * 1994-07-08 1996-02-09 Schering Corporation Method for identifying nucleic acids encoding c-fos promoter activating proteins
DE4428402A1 (de) * 1994-08-11 1996-02-15 Boehringer Mannheim Gmbh Gentherapeutisches Verfahren unter Verwendung von DNA-Vektoren ohne Selektionsmarker-Gen
US5792647A (en) * 1995-02-13 1998-08-11 The Johns Hopkins University Bacterial catabolism of chitin
US6254874B1 (en) * 1995-04-13 2001-07-03 President And Fellows Of Harvard College Attenuated auxotrophic microorganisms having a combination of non-attenuating mutations and method for making same
US5763270A (en) * 1995-06-07 1998-06-09 Genemedicine, Inc. Plasmid for delivery of nucleic acids to cells and methods of use
US7279313B2 (en) * 1995-09-15 2007-10-09 Centelion Circular DNA molecule having a conditional origin of replication, process for their preparation and their use in gene therapy
FR2738842B1 (fr) * 1995-09-15 1997-10-31 Rhone Poulenc Rorer Sa Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique
US7364894B2 (en) * 1995-09-15 2008-04-29 Centelion Circular DNA molecule having a conditional origin of replication, process for their preparation and their use in gene therapy
US5830879A (en) * 1995-10-02 1998-11-03 St. Elizabeth's Medical Center Of Boston, Inc. Treatment of vascular injury using vascular endothelial growth factor
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US5874259A (en) * 1997-11-21 1999-02-23 Wisconsin Alumni Research Foundation Conditionally amplifiable BAC vector

Also Published As

Publication number Publication date
FR2738842A1 (fr) 1997-03-21
EP1724354A3 (de) 2008-10-29
EP0850310A1 (de) 1998-07-01
CA2229307C (fr) 2013-06-11
HU224400B1 (hu) 2005-08-29
PT850310E (pt) 2005-05-31
BR9610511A (pt) 1999-01-26
JP2007325603A (ja) 2007-12-20
FR2738842B1 (fr) 1997-10-31
PT1724354E (pt) 2011-03-09
IL207348A0 (en) 2010-12-30
DE69638314D1 (de) 2011-02-03
EP1508620A1 (de) 2005-02-23
ATE352630T1 (de) 2007-02-15
DE69634043T2 (de) 2005-08-18
SK285317B6 (sk) 2006-10-05
AU728231B2 (en) 2001-01-04
CZ78898A3 (cs) 1998-06-17
HUP9900018A3 (en) 2001-10-29
US6977174B2 (en) 2005-12-20
US20010014476A1 (en) 2001-08-16
ES2233975T3 (es) 2005-06-16
ES2281721T3 (es) 2007-10-01
NO981044L (no) 1998-03-10
JP3818665B2 (ja) 2006-09-06
AU6992496A (en) 1997-04-01
EP0850310B1 (de) 2004-12-15
KR19990044683A (ko) 1999-06-25
HUP9900018A1 (hu) 1999-04-28
EP1724354A2 (de) 2006-11-22
EP1508620B1 (de) 2007-01-24
JP2006136328A (ja) 2006-06-01
IL173399A0 (en) 2006-06-11
BRPI9610511B8 (pt) 2016-11-08
NO323975B1 (no) 2007-07-30
JP4756014B2 (ja) 2011-08-24
SK34798A3 (en) 1998-11-04
DK0850310T3 (da) 2005-04-25
MX9802005A (es) 1998-08-30
EP1724354B1 (de) 2010-12-22
JP4585435B2 (ja) 2010-11-24
DK1724354T3 (da) 2011-04-04
NO981044D0 (no) 1998-03-10
DE69636878T2 (de) 2007-11-08
ES2357098T3 (es) 2011-04-18
US20090149406A1 (en) 2009-06-11
CZ294525B6 (cs) 2005-01-12
ATE492642T1 (de) 2011-01-15
JP2000501281A (ja) 2000-02-08
TWI231826B (en) 2005-05-01
US20090130674A1 (en) 2009-05-21
DE69634043D1 (de) 2005-01-20
WO1997010343A1 (fr) 1997-03-20
DE69636878D1 (de) 2007-03-15
IL123641A0 (en) 1998-10-30
IL123641A (en) 2006-06-11
BR9610511B1 (pt) 2010-10-19
PT1508620E (pt) 2007-05-31
IL173399A (en) 2010-11-30
CA2229307A1 (fr) 1997-03-20
ZA967640B (en) 1997-03-25
DK1508620T3 (da) 2007-05-29
KR100522369B1 (ko) 2006-10-24

Similar Documents

Publication Publication Date Title
ATE352630T1 (de) Zirkuläres dna molekül mit einem abhängigen replikationsursprung, seine herstellungsverfahren und seine verwendung in der gentherapie
WO1996021007A3 (en) Bacteriophage-mediated gene transfer systems capable of transfecting eukaryotic cells
IL118201A0 (en) Erythropoietin free of animal proteins processes for production and use thereof
AU560340B2 (en) Rennin from recombinant dna
EA199700364A1 (ru) Способ контроля силилирования протеинов, продуцируемых в культуре клеток млекопитающих
ATE298789T1 (de) Negativstrand rna virus mit selbständiger replikationsaktivität
DE69836092D1 (de) Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
ATE159297T1 (de) Expression von menschlichen serumalbumin in methylotrophischen hefen
DE69430975T2 (de) Verfahren zur Herstellung von Proteinen
CA2037677A1 (en) Process for production of exogenous gene or its product in plant cells
ES2078243T3 (es) Mejoras en o relativas a la expresion eucariotica.
ES2070125T3 (es) Factor de crecimiento fibroblastico acido mutante.
DE69331216T2 (de) Planze resistent gegen mindestens zwei viren und dessen preparation
RU94043698A (ru) СПОСОБ ПОЛУЧЕНИЯ ФЕРМЕНТА ДЕСТАБИЛАЗЫ, ОБЛАДАЮЩЕГО ФИБРИНОЛИТИЧЕСКОЙ, ТРОМБОЛИТИЧЕСКОЙ, ЭНДО- И ЭКЗО- Е( -glu)-Lys-ИЗОПЕПТИДАЗНОЙ И АМИДОЛИТИЧЕСКОЙ АКТИВНОСТЬЮ
EP0688224A4 (de) Ein mutiertes protein und verfahren und materialien zu seiner herstellung und verwendung
ATE288476T1 (de) Herstellung von proteinen
SE8903865D0 (sv) Expression of heterologous proteins using eukaryotic cells
DE69105768D1 (de) Verfahren zur Herstellung von Fremdprotein in Escherichia Coli.
DE3688974D1 (de) Expressionssysteme zur überproduktion gewünschter proteine.

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 0850310

Country of ref document: EP