ATE206210T1 - Fluoreszenzfarbstoff, pyryliumsalze oder ähnhliche salze enthaltend, nachweisverfahren von nukleinsäure durch verwendung desselben - Google Patents

Fluoreszenzfarbstoff, pyryliumsalze oder ähnhliche salze enthaltend, nachweisverfahren von nukleinsäure durch verwendung desselben

Info

Publication number
ATE206210T1
ATE206210T1 AT93120508T AT93120508T ATE206210T1 AT E206210 T1 ATE206210 T1 AT E206210T1 AT 93120508 T AT93120508 T AT 93120508T AT 93120508 T AT93120508 T AT 93120508T AT E206210 T1 ATE206210 T1 AT E206210T1
Authority
AT
Austria
Prior art keywords
nucleic acid
pyrylium
salts
stain
substituted
Prior art date
Application number
AT93120508T
Other languages
English (en)
Inventor
Nobuko Yamamoto
Tadashi Okamoto
Yoshinori Tomida
Takeshi Miyazaki
Masahiro Kawaguchi
Original Assignee
Canon Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Canon Kk filed Critical Canon Kk
Application granted granted Critical
Publication of ATE206210T1 publication Critical patent/ATE206210T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B57/00—Other synthetic dyes of known constitution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/10—DNA staining
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/173—Nucleic acid detection characterized by the use of physical, structural and functional properties staining/intercalating agent, e.g. ethidium bromide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Heterocyclic Compounds Containing Sulfur Atoms (AREA)
  • Pyrane Compounds (AREA)
  • Luminescent Compositions (AREA)
AT93120508T 1992-12-21 1993-12-20 Fluoreszenzfarbstoff, pyryliumsalze oder ähnhliche salze enthaltend, nachweisverfahren von nukleinsäure durch verwendung desselben ATE206210T1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP34075592 1992-12-21
JP4953093 1993-03-10
JP22720493 1993-09-13
JP26686693 1993-10-26
JP31804593A JP3247001B2 (ja) 1992-12-21 1993-12-17 ピリリウム化合物を用いた２本鎖核酸の検出方法、ピリリウム化合物を含有するプローブ及びそれを用いた標的核酸の検出方法、新規ピリリウム化合物

Publications (1)

Publication Number Publication Date
ATE206210T1 true ATE206210T1 (de) 2001-10-15

Family

ID=27522809

Family Applications (2)

Application Number Title Priority Date Filing Date
AT00112804T ATE343643T1 (de) 1992-12-21 1993-12-20 Fluoreszenzfarbstoff, der pyryliumsalze oder ähnliche salze enthält, nachweisverfahren von nukleinsäuren durch seine verwendung
AT93120508T ATE206210T1 (de) 1992-12-21 1993-12-20 Fluoreszenzfarbstoff, pyryliumsalze oder ähnhliche salze enthaltend, nachweisverfahren von nukleinsäure durch verwendung desselben

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT00112804T ATE343643T1 (de) 1992-12-21 1993-12-20 Fluoreszenzfarbstoff, der pyryliumsalze oder ähnliche salze enthält, nachweisverfahren von nukleinsäuren durch seine verwendung

Country Status (6)

Country Link
US (2) US5624798A (de)
EP (2) EP1052291B1 (de)
JP (1) JP3247001B2 (de)
AT (2) ATE343643T1 (de)
DE (2) DE69330818T2 (de)
SG (1) SG65577A1 (de)

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JP4458327B2 (ja) * 2003-08-28 2010-04-28 キヤノン株式会社 標的物質の定量方法および該方法に用いるプローブ担体
JP4632400B2 (ja) * 2003-12-16 2011-02-16 キヤノン株式会社 細胞培養用基板、その製造方法、それを用いた細胞スクリーニング法
EP1694812A4 (de) * 2003-12-16 2008-11-12 Canon Kk Blatt zur übertragung biologisch aktiver substanzen, aus einer zellkulturplatte und dem blatt zur übertragung biologisch aktiver substanzen bestehender zellkultur-kit, herstellungsverfahren dafür und verfahren zum screening von zellkulturbedingungen unter verwendung davon
JP4541731B2 (ja) * 2004-03-12 2010-09-08 キヤノン株式会社 核酸検出方法
JP4183256B2 (ja) * 2004-08-04 2008-11-19 キヤノン株式会社 核酸増幅反応産物の鎖分離方法、核酸増幅反応産物の検出方法
JP4147235B2 (ja) * 2004-09-27 2008-09-10 キヤノン株式会社 吐出用液体、吐出方法、液滴化方法、液体吐出カートリッジ及び吐出装置
JP2006094760A (ja) * 2004-09-29 2006-04-13 Canon Inc 細胞のスクリーニング方法および口腔癌細胞検出方法
US8535914B2 (en) * 2005-01-21 2013-09-17 Canon Kabushiki Kaisha Probe, probe set and information acquisition method using the same
JP4689340B2 (ja) * 2005-05-02 2011-05-25 キヤノン株式会社 吐出用液体医薬組成物
US7678538B2 (en) * 2005-05-13 2010-03-16 American National Red Cross Disinfection of biological fluids using asymmetric cyanine dyes
JP2007010413A (ja) * 2005-06-29 2007-01-18 Canon Inc 核酸ハイブリッドの融点測定方法及びそのための装置
US7642086B2 (en) * 2005-08-09 2010-01-05 Canon Kabushiki Kaisha Labeled probe bound object, method for producing the same and method for using the same
US20090011413A1 (en) * 2005-12-14 2009-01-08 Canon Kabushiki Kaisha Method for screening colon cancer cells and gene set used for examination of colon cancer
WO2007114516A1 (en) * 2006-03-31 2007-10-11 Canon Kabushiki Kaisha Probe, probe set, probe-immobilized carrier, and genetic testing method
US8187803B2 (en) * 2006-03-31 2012-05-29 Canon Kabushiki Kaisha Probe, probe set, probe-immobilized carrier, and genetic testing method
US20100112545A1 (en) * 2007-07-13 2010-05-06 Subra Muralidharan Trans-1,2-diphenylethlene derivatives and nanosensors made therefrom
JP5688899B2 (ja) 2008-12-25 2015-03-25 キヤノン株式会社 生物試料用標識剤並びに該標識剤を用いた標識方法及びスクリーニング方法
JP5733734B2 (ja) 2009-02-19 2015-06-10 国立大学法人 千葉大学 成長因子に誘発されるユークロマチン化（ｅｕｃｈｒｏｍａｔｉｎｉｚａｔｉｏｎ）に必要な、ＳＲＣ−ファミリーチロシンキナーゼの核局在
JP5729904B2 (ja) 2009-06-02 2015-06-03 キヤノン株式会社 細胞から蛋白質、ｄｎａ、ｒｎａを調製する方法
CN113072534B (zh) * 2021-04-02 2022-02-08 广西师范大学 一种rna荧光探针及其制备方法和应用
CN113281313B (zh) * 2021-04-16 2023-05-09 安徽大学 实时定量细胞核内rna输出的生物荧光探针及其制备方法

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Also Published As

Publication number Publication date
DE69330818T2 (de) 2002-03-28
DE69330818D1 (de) 2001-10-31
DE69334079T2 (de) 2007-05-16
ATE343643T1 (de) 2006-11-15
EP1052291A2 (de) 2000-11-15
JPH07174759A (ja) 1995-07-14
EP1052291A3 (de) 2000-11-22
US6022961A (en) 2000-02-08
EP0603783B1 (de) 2001-09-26
US5624798A (en) 1997-04-29
SG65577A1 (en) 1999-06-22
DE69334079D1 (de) 2006-12-07
EP1052291B1 (de) 2006-10-25
EP0603783A1 (de) 1994-06-29
JP3247001B2 (ja) 2002-01-15

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