SG65577A1 - Fluorescent stain containing pyrylium salt or its similar salt detection method of nucleic acid by use of it and fluorescent staining method of biological sample - Google Patents

Fluorescent stain containing pyrylium salt or its similar salt detection method of nucleic acid by use of it and fluorescent staining method of biological sample

Info

Publication number
SG65577A1
SG65577A1 SG1996006532A SG1996006532A SG65577A1 SG 65577 A1 SG65577 A1 SG 65577A1 SG 1996006532 A SG1996006532 A SG 1996006532A SG 1996006532 A SG1996006532 A SG 1996006532A SG 65577 A1 SG65577 A1 SG 65577A1
Authority
SG
Singapore
Prior art keywords
nucleic acid
fluorescent
salt
biological sample
pyrylium
Prior art date
Application number
SG1996006532A
Other languages
English (en)
Inventor
Masahiro Kawaguchi
Nobuko Yamamoto
Tadashi Okamoto
Takeshi Miyazaki
Yoshinori Tomida
Original Assignee
Canon Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Canon Kk filed Critical Canon Kk
Publication of SG65577A1 publication Critical patent/SG65577A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B57/00—Other synthetic dyes of known constitution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/10—DNA staining
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/173—Nucleic acid detection characterized by the use of physical, structural and functional properties staining/intercalating agent, e.g. ethidium bromide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Luminescent Compositions (AREA)
  • Heterocyclic Compounds Containing Sulfur Atoms (AREA)
  • Pyrane Compounds (AREA)
SG1996006532A 1992-12-21 1993-12-20 Fluorescent stain containing pyrylium salt or its similar salt detection method of nucleic acid by use of it and fluorescent staining method of biological sample SG65577A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP34075592 1992-12-21
JP4953093 1993-03-10
JP22720493 1993-09-13
JP26686693 1993-10-26
JP31804593A JP3247001B2 (ja) 1992-12-21 1993-12-17 ピリリウム化合物を用いた２本鎖核酸の検出方法、ピリリウム化合物を含有するプローブ及びそれを用いた標的核酸の検出方法、新規ピリリウム化合物

Publications (1)

Publication Number Publication Date
SG65577A1 true SG65577A1 (en) 1999-06-22

Family

ID=27522809

Family Applications (1)

Application Number Title Priority Date Filing Date
SG1996006532A SG65577A1 (en) 1992-12-21 1993-12-20 Fluorescent stain containing pyrylium salt or its similar salt detection method of nucleic acid by use of it and fluorescent staining method of biological sample

Country Status (6)

Country Link
US (2) US5624798A (de)
EP (2) EP1052291B1 (de)
JP (1) JP3247001B2 (de)
AT (2) ATE206210T1 (de)
DE (2) DE69334079T2 (de)
SG (1) SG65577A1 (de)

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US7332599B2 (en) * 2003-06-06 2008-02-19 North Carolina State University Methods and intermediates for the synthesis of dipyrrin-substituted porphyrinic macrocycles
US7057100B2 (en) * 2003-06-26 2006-06-06 The J.C. Robinson Seed Co. Inbred corn line W23129
JP4458327B2 (ja) * 2003-08-28 2010-04-28 キヤノン株式会社 標的物質の定量方法および該方法に用いるプローブ担体
WO2005059086A1 (en) * 2003-12-16 2005-06-30 Canon Kabushiki Kaisha Biologically active substance transfer sheet, cell culture kit constituted of cell culture plate and biologically active substance transfer sheet, producing method thereof and method for screening cell culture conditions utilizing the same
JP4632400B2 (ja) * 2003-12-16 2011-02-16 キヤノン株式会社 細胞培養用基板、その製造方法、それを用いた細胞スクリーニング法
JP4541731B2 (ja) * 2004-03-12 2010-09-08 キヤノン株式会社 核酸検出方法
JP4183256B2 (ja) * 2004-08-04 2008-11-19 キヤノン株式会社 核酸増幅反応産物の鎖分離方法、核酸増幅反応産物の検出方法
JP4147235B2 (ja) * 2004-09-27 2008-09-10 キヤノン株式会社 吐出用液体、吐出方法、液滴化方法、液体吐出カートリッジ及び吐出装置
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US8535914B2 (en) * 2005-01-21 2013-09-17 Canon Kabushiki Kaisha Probe, probe set and information acquisition method using the same
JP4689340B2 (ja) * 2005-05-02 2011-05-25 キヤノン株式会社 吐出用液体医薬組成物
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US7642086B2 (en) * 2005-08-09 2010-01-05 Canon Kabushiki Kaisha Labeled probe bound object, method for producing the same and method for using the same
US20090011413A1 (en) * 2005-12-14 2009-01-08 Canon Kabushiki Kaisha Method for screening colon cancer cells and gene set used for examination of colon cancer
WO2007114517A1 (en) * 2006-03-31 2007-10-11 Canon Kabushiki Kaisha Probe, probe set, probe-immobilized carrier, and genetic testing method
US8133668B2 (en) * 2006-03-31 2012-03-13 Canon Kabushiki Kaisha Probe, probe set, probe-immobilized carrier, and genetic testing method
WO2009011674A1 (en) * 2007-07-13 2009-01-22 Western Michigan University Research Foundation Trans-1,2-diphenylethylene derivatives and nanosensors made therefrom
WO2010074326A1 (en) 2008-12-25 2010-07-01 Canon Kabushiki Kaisha Probe for a biological specimen and labelling method and screening method using the probe
JP5733734B2 (ja) 2009-02-19 2015-06-10 国立大学法人 千葉大学 成長因子に誘発されるユークロマチン化（ｅｕｃｈｒｏｍａｔｉｎｉｚａｔｉｏｎ）に必要な、ＳＲＣ−ファミリーチロシンキナーゼの核局在
JP5729904B2 (ja) 2009-06-02 2015-06-03 キヤノン株式会社 細胞から蛋白質、ｄｎａ、ｒｎａを調製する方法
CN113072534B (zh) * 2021-04-02 2022-02-08 广西师范大学 一种rna荧光探针及其制备方法和应用
CN113281313B (zh) * 2021-04-16 2023-05-09 安徽大学 实时定量细胞核内rna输出的生物荧光探针及其制备方法

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Also Published As

Publication number Publication date
US5624798A (en) 1997-04-29
JPH07174759A (ja) 1995-07-14
EP0603783A1 (de) 1994-06-29
DE69330818T2 (de) 2002-03-28
EP1052291B1 (de) 2006-10-25
ATE206210T1 (de) 2001-10-15
JP3247001B2 (ja) 2002-01-15
DE69334079T2 (de) 2007-05-16
DE69334079D1 (de) 2006-12-07
DE69330818D1 (de) 2001-10-31
EP0603783B1 (de) 2001-09-26
US6022961A (en) 2000-02-08
EP1052291A3 (de) 2000-11-22
ATE343643T1 (de) 2006-11-15
EP1052291A2 (de) 2000-11-15

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