AR096027A2 - Proteínas insecticidas quiméricas de bacillus thuringiensis - Google Patents
Proteínas insecticidas quiméricas de bacillus thuringiensisInfo
- Publication number
- AR096027A2 AR096027A2 ARP140101586A ARP140101586A AR096027A2 AR 096027 A2 AR096027 A2 AR 096027A2 AR P140101586 A ARP140101586 A AR P140101586A AR P140101586 A ARP140101586 A AR P140101586A AR 096027 A2 AR096027 A2 AR 096027A2
- Authority
- AR
- Argentina
- Prior art keywords
- amino acid
- acid sequence
- proteins
- lmbp
- cry2ae
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 11
- 102000004169 proteins and genes Human genes 0.000 title abstract 9
- 241000193388 Bacillus thuringiensis Species 0.000 title abstract 2
- 229940097012 bacillus thuringiensis Drugs 0.000 title abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 7
- 230000000749 insecticidal effect Effects 0.000 abstract 4
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 3
- 108091026890 Coding region Proteins 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 2
- 150000007523 nucleic acids Chemical group 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000002917 insecticide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Compuestos insecticidas derivados de cepas de Bacillus thuringiensis. Se proveen proteínas denominadas Cry2Ae, Cry2Af y Cry2Ag, y variantes de las mismas, así como secuencias de ADN codificadores de estas proteínas o de las variantes de las mismas. Además se proveen huéspedes recombinantes que expresan dichas proteínas, en particular células vegetales y plantas. Reivindicación 1: Un gen quimérico que comprende una secuencia de ácido nucleico bajo el control de un promotor de expresión en plantas, caracterizada porque dicha secuencia de ácido nucleico codifica una proteína insecticida Cry2Ae que comprende una secuencia de aminoácidos seleccionada del grupo formado por : a) la secuencia de aminoácidos de la proteína codificada por el gen cry2Ae depositado en BCCM-LMBP con el Nº Acceso LMBP 4248 o una porción insecticida de la misma, b) la secuencia de aminoácidos de la proteína de la SEQ ID Nº 2, o una porción insecticida de la misma, c) la secuencia de aminoácidos de la proteína de la SEQ ID Nº 2 entre la posición de aminoácido 1 y la posición de aminoácido 625 y d) la secuencia de aminoácidos de una proteína insecticida codificada por un ADN que se hibridiza con la secuencia de codificación cry2Ae depositada en BCCM-LMBP con el Nº Acceso LMBP 4248 bajo condiciones de hibridación severas, donde dicho ADN presenta una identidad de secuencia de por lo menos 93%, en particular por lo menos 98%, preferentemente por lo menos 99%, con la secuencia de codificación de la SEQ ID Nº 1.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US75629601A | 2001-01-09 | 2001-01-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR096027A2 true AR096027A2 (es) | 2015-12-02 |
Family
ID=25042854
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP020100051A AR032231A1 (es) | 2001-01-09 | 2002-01-09 | Nuevas proteinas insecticidas de bacillus thuringiensis |
| ARP140101586A AR096027A2 (es) | 2001-01-09 | 2014-04-15 | Proteínas insecticidas quiméricas de bacillus thuringiensis |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP020100051A AR032231A1 (es) | 2001-01-09 | 2002-01-09 | Nuevas proteinas insecticidas de bacillus thuringiensis |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP1352068B1 (es) |
| JP (1) | JP4404549B2 (es) |
| CN (1) | CN1234867C (es) |
| AR (2) | AR032231A1 (es) |
| AT (1) | ATE409231T1 (es) |
| AU (1) | AU2002252974B2 (es) |
| BR (3) | BR122015003517B1 (es) |
| CA (2) | CA2433817C (es) |
| DE (1) | DE60229037D1 (es) |
| ES (1) | ES2315360T3 (es) |
| MX (1) | MXPA03006130A (es) |
| PT (1) | PT1352068E (es) |
| SI (1) | SI1352068T1 (es) |
| WO (1) | WO2002057664A2 (es) |
Families Citing this family (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU7491600A (en) | 1999-09-15 | 2001-04-17 | Monsanto Technology Llc | Lepidopteran-active bacillus thuringiensis delta-endotoxin compositions and methods of use |
| CN100497378C (zh) | 2002-03-22 | 2009-06-10 | 拜尔生物科学公司 | 新的苏云金芽孢杆菌杀昆虫蛋白质 |
| CN101671680B (zh) * | 2004-02-25 | 2012-11-28 | 先锋高级育种国际公司 | 新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物 |
| AU2006231503A1 (en) * | 2005-04-05 | 2006-10-12 | E. I. Du Pont De Nemours And Company | Methods and compositions for designing nucleic acid molecules for polypeptide expression in plants using plant virus codon-bias |
| CN101300268A (zh) | 2005-07-08 | 2008-11-05 | 墨西哥国立自治大学 | 新的具有杀虫活性的细菌蛋白质 |
| AU2007228981B2 (en) * | 2006-03-21 | 2012-10-04 | BASF Agricultural Solutions Seed US LLC | Novel genes encoding insecticidal proteins |
| US9994621B2 (en) | 2007-06-01 | 2018-06-12 | Bayer Cropscience N.V. | Genes encoding insecticidal proteins |
| EP2615173B1 (en) * | 2007-06-11 | 2020-09-16 | Basf Agricultural Solutions Seed Us Llc | Insect resistant cotton plants and methods for identifying same |
| US7772465B2 (en) | 2007-06-26 | 2010-08-10 | Pioneer Hi-Bred International, Inc. | Bacillus thuringiensis gene with lepidopteran activity |
| AR068894A1 (es) * | 2007-10-16 | 2009-12-16 | Athenix Corp | Proteinas delta- endotoxina axmi-066 y axmi-076 y metodos de uso de las mismas |
| BRPI0914615A2 (pt) * | 2008-06-13 | 2016-09-06 | Bayer Bioscience Nv | administração de resistência de inseto de lagarta em plantas transgênicas |
| CA2754845A1 (en) * | 2009-03-11 | 2010-12-09 | Athenix Corporation | Axmi-030 insecticidal protein from bacillus thuringiensis and methods for use |
| AU2010330919B2 (en) * | 2009-12-16 | 2016-05-12 | Dow Agrosciences Llc | Insecticidal protein combination comprising Cry1Ab and Cry2Aa for controlling European corn borer, and methods for insect resistance management |
| AR079882A1 (es) | 2009-12-23 | 2012-02-29 | Bayer Cropscience Ag | Plantas tolerantes a herbicidas inhibidores de las hppd |
| UY33141A (es) | 2009-12-23 | 2011-07-29 | Bayer Cropscience Ag | Plantas tolerantes a herbicidas inhibidores de las hppd |
| UY33140A (es) | 2009-12-23 | 2011-07-29 | Bayer Cropscience Ag | Plantas tolerantes a herbicidas inhibidores de las hppd |
| AR079883A1 (es) | 2009-12-23 | 2012-02-29 | Bayer Cropscience Ag | Plantas tolerantes a herbicidas inhibidores de las hppd |
| ES2658990T3 (es) | 2009-12-23 | 2018-03-13 | Bayer Intellectual Property Gmbh | Plantas tolerantes a herbicidas inhibidores de HPPD |
| EP2669371A1 (en) | 2010-11-10 | 2013-12-04 | Bayer CropScience AG | HPPD variants and methods of use |
| US8648230B2 (en) | 2011-03-18 | 2014-02-11 | Ms Technologies, Llc | Regulatory regions preferentially expressing in non-pollen plant tissue |
| PL2688406T3 (pl) | 2011-03-25 | 2015-10-30 | Bayer Ip Gmbh | Zastosowanie N-(tetrazol-4-ilo)- lub N-(triazol-3-ilo)arylokarboksamidów lub ich soli do zwalczania niepożądanych roślin w obszarach transgenicznych roślin uprawnych tolerancyjnych na środki chwastobójcze będące inhibitorem HPPD |
| EA201391302A1 (ru) | 2011-03-25 | 2014-04-30 | Байер Интеллектуэль Проперти Гмбх | Применение n-(1,2,5-оксадиазол-3-ил)бензамидов для борьбы с нежелательными растениями в районах произрастания трансгенных культурных растений, устойчивых к гербицидам - ингибиторам hppd |
| US9567381B2 (en) | 2012-03-09 | 2017-02-14 | Vestaron Corporation | Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides |
| US11692016B2 (en) | 2012-03-09 | 2023-07-04 | Vestaron Corporation | High gene expression yeast strain |
| CN103571853B (zh) * | 2012-08-08 | 2016-01-20 | 中国科学院亚热带农业生态研究所 | 密码子优化的Cry2Aa基因与重组载体以及改变作物抗性的方法 |
| CN104736699B (zh) | 2012-09-14 | 2020-04-14 | 拜尔作物科学有限合伙公司 | Hppd变体及使用方法 |
| WO2014138339A2 (en) | 2013-03-07 | 2014-09-12 | Athenix Corp. | Toxin genes and methods for their use |
| CN103290130B (zh) * | 2013-06-13 | 2015-02-04 | 中华人民共和国上海出入境检验检疫局 | 转基因产品中Cry2Ae基因的检测方法及其试剂盒 |
| MX2016011745A (es) | 2014-03-11 | 2017-09-01 | Bayer Cropscience Lp | Variantes de hppd y metodos de uso. |
| CN104286014B (zh) * | 2014-08-27 | 2016-03-23 | 北京大北农科技集团股份有限公司 | 杀虫蛋白的用途 |
| CN105367633B (zh) * | 2014-08-28 | 2018-11-30 | 四川农业大学 | 一种BT蛋白CRY2Ab32、其编码基因及应用 |
| AR105155A1 (es) | 2015-07-07 | 2017-09-13 | Syngenta Participations Ag | Composiciones y métodos para controlar plagas de plantas |
| MX2018003044A (es) | 2015-09-11 | 2018-04-11 | Bayer Cropscience Ag | Variantes de hppd y metodos de uso. |
| US11535653B2 (en) | 2016-10-21 | 2022-12-27 | Vestaron Corporation | Cleavable peptides and insecticidal and nematicidal proteins comprising same |
| US11091772B2 (en) | 2016-11-23 | 2021-08-17 | BASF Agricultural Solutions Seed US LLC | AXMI669 and AXMI991 toxin genes and methods for their use |
| WO2018119336A1 (en) | 2016-12-22 | 2018-06-28 | Athenix Corp. | Use of cry14 for the control of nematode pests |
| EP3571303A1 (en) | 2017-01-18 | 2019-11-27 | Basf Agricultural Solutions Seed Us Llc | Bp005 toxin gene and methods for its use |
| WO2018136611A1 (en) | 2017-01-18 | 2018-07-26 | Bayer Cropscience Lp | Use of bp005 for the control of plant pathogens |
| US11708565B2 (en) | 2017-03-07 | 2023-07-25 | BASF Agricultural Solutions Seesi US LLC | HPPD variants and methods of use |
| BR112020008096A2 (pt) | 2017-10-24 | 2020-11-03 | Basf Se | método para conferir tolerância a um herbicida e planta de soja transgênica |
| BR112020008092A2 (pt) | 2017-10-24 | 2020-09-15 | BASF Agricultural Solutions Seed US LLC | método para conferir tolerância a um herbicida e planta de soja transgênica |
| US20240287145A1 (en) * | 2019-04-24 | 2024-08-29 | Dcm Shriram Limited | CODON OPTIMIZED SYNTHETIC NUCLEOTIDE SEQUENCES ENCODING CRY2Ai PROTEIN AND USES THEREOF |
| US20220275031A1 (en) * | 2019-07-30 | 2022-09-01 | Dcm Shriram Limited | Synthetic nucleotide sequences encoding insecticidal crystal protein and uses thereof |
| CN111334586A (zh) * | 2020-04-02 | 2020-06-26 | 南京农业大学 | 基于as-pcr技术快速检测二化螟鱼尼丁受体基因突变的方法 |
| UY39585A (es) * | 2020-12-23 | 2022-07-29 | Monsanto Technology Llc | Proteínas que exhiben actividad inhibidora de insectos frente a plagas con importancia agrícola de plantas de cultivo y semillas |
| CN114717256A (zh) * | 2022-02-19 | 2022-07-08 | 四川农业大学 | 在水稻中高效表达Bt蛋自Cry2Ag1抗草地贪夜蛾的方法 |
| WO2024137446A1 (en) | 2022-12-19 | 2024-06-27 | BASF Agricultural Solutions Seed US LLC | Methods of identifying and evaluating genes for insect control |
| WO2024137438A2 (en) | 2022-12-19 | 2024-06-27 | BASF Agricultural Solutions Seed US LLC | Insect toxin genes and methods for their use |
| WO2024137445A1 (en) | 2022-12-20 | 2024-06-27 | BASF Agricultural Solutions Seed US LLC | Methods of identifying and evaluating genes for insect control |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL177516C (nl) | 1978-09-12 | 1985-10-01 | Pidou Bv | Afdichtmanchet. |
| NL8700204A (nl) | 1987-01-28 | 1988-08-16 | Pidou Bv | Afdichtinrichting. |
| JP2001522233A (ja) * | 1997-03-13 | 2001-11-13 | マイコーゲン コーポレーション | バチルス・チューリンギエンシス毒素 |
| US6489542B1 (en) * | 1998-11-04 | 2002-12-03 | Monsanto Technology Llc | Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids |
| AU7491600A (en) * | 1999-09-15 | 2001-04-17 | Monsanto Technology Llc | Lepidopteran-active bacillus thuringiensis delta-endotoxin compositions and methods of use |
-
2002
- 2002-01-08 BR BR122015003517-1A patent/BR122015003517B1/pt active IP Right Grant
- 2002-01-08 WO PCT/EP2002/000298 patent/WO2002057664A2/en active IP Right Grant
- 2002-01-08 CA CA2433817A patent/CA2433817C/en not_active Expired - Lifetime
- 2002-01-08 DE DE60229037T patent/DE60229037D1/de not_active Expired - Lifetime
- 2002-01-08 CA CA2771117A patent/CA2771117C/en not_active Expired - Lifetime
- 2002-01-08 BR BRPI0206346-8A patent/BRPI0206346B1/pt unknown
- 2002-01-08 CN CNB028035674A patent/CN1234867C/zh not_active Expired - Lifetime
- 2002-01-08 ES ES02722023T patent/ES2315360T3/es not_active Expired - Lifetime
- 2002-01-08 PT PT02722023T patent/PT1352068E/pt unknown
- 2002-01-08 AU AU2002252974A patent/AU2002252974B2/en not_active Expired
- 2002-01-08 AT AT02722023T patent/ATE409231T1/de active
- 2002-01-08 JP JP2002557703A patent/JP4404549B2/ja not_active Expired - Lifetime
- 2002-01-08 MX MXPA03006130A patent/MXPA03006130A/es active IP Right Grant
- 2002-01-08 EP EP02722023A patent/EP1352068B1/en not_active Expired - Lifetime
- 2002-01-08 BR BR0206346-8A patent/BR0206346A/pt active IP Right Grant
- 2002-01-08 SI SI200230777T patent/SI1352068T1/sl unknown
- 2002-01-09 AR ARP020100051A patent/AR032231A1/es active IP Right Grant
-
2014
- 2014-04-15 AR ARP140101586A patent/AR096027A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2771117C (en) | 2015-03-17 |
| AR032231A1 (es) | 2003-10-29 |
| CN1484702A (zh) | 2004-03-24 |
| ES2315360T3 (es) | 2009-04-01 |
| ATE409231T1 (de) | 2008-10-15 |
| MXPA03006130A (es) | 2005-02-14 |
| CN1234867C (zh) | 2006-01-04 |
| WO2002057664A3 (en) | 2003-04-17 |
| WO2002057664A2 (en) | 2002-07-25 |
| CA2433817C (en) | 2012-05-22 |
| JP2004522437A (ja) | 2004-07-29 |
| BRPI0206346B1 (pt) | 2018-04-03 |
| EP1352068B1 (en) | 2008-09-24 |
| AU2002252974B2 (en) | 2006-12-14 |
| CA2771117A1 (en) | 2002-07-25 |
| BR0206346A (pt) | 2003-10-28 |
| EP1352068A2 (en) | 2003-10-15 |
| CA2433817A1 (en) | 2002-07-25 |
| SI1352068T1 (sl) | 2009-02-28 |
| PT1352068E (pt) | 2009-01-06 |
| JP4404549B2 (ja) | 2010-01-27 |
| DE60229037D1 (de) | 2008-11-06 |
| BR122015003517B1 (pt) | 2021-02-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |