AR038725A1 - Novedosas toxinas vip3, procedentes de bacillus thurigiensis y metodos para su utilizacion - Google Patents
Novedosas toxinas vip3, procedentes de bacillus thurigiensis y metodos para su utilizacionInfo
- Publication number
- AR038725A1 AR038725A1 ARP030100713A ARP030100713A AR038725A1 AR 038725 A1 AR038725 A1 AR 038725A1 AR P030100713 A ARP030100713 A AR P030100713A AR P030100713 A ARP030100713 A AR P030100713A AR 038725 A1 AR038725 A1 AR 038725A1
- Authority
- AR
- Argentina
- Prior art keywords
- seq
- nucleotide sequence
- toxin
- vip3
- procedures
- Prior art date
Links
- 239000003053 toxin Substances 0.000 title abstract 5
- 231100000765 toxin Toxicity 0.000 title abstract 5
- 108700012359 toxins Proteins 0.000 title abstract 5
- 238000000034 method Methods 0.000 title 2
- 241000193830 Bacillus <bacterium> Species 0.000 title 1
- 101150077913 VIP3 gene Proteins 0.000 title 1
- 239000002773 nucleotide Substances 0.000 abstract 4
- 125000003729 nucleotide group Chemical group 0.000 abstract 4
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 abstract 3
- 241000238631 Hexapoda Species 0.000 abstract 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 abstract 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 abstract 1
- 241000255777 Lepidoptera Species 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Pest Control & Pesticides (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Dentistry (AREA)
- Cell Biology (AREA)
- Insects & Arthropods (AREA)
- Gastroenterology & Hepatology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Se revelan novedosas toxinas Vip3 que son sumamente activas contra un amplio espectro de insectos plaga lepidópteros. El ADN que codifuca la toxina Vip3 puede ser utilizado para transformar diversos organismos procariotas y eucariotas para expresar la toxina Vip3. Dichos organismos recombinantes pueden ser utilizados para controlar los insectos lepidópteros en diversos entornos. Reivindicación (1): Una molécula aislada de ácidos nucléicos, que comprende una secuencia de nucleótidos que codifica una toxina que es activa contra los insectos, en la cual dicha secuencia de nucleótidos: (a) - tiene un complemento que se hibrida con los nucleótidos 1981-2367 de la SEQ ID NO: 1 en dodecilsulfato de sodio (SDS) al 7%, NaPO4 0,5 M, EDTA 1 mM a 50 °C, con un lavado en 0,1 XSSC, SDS al 0,1%, a 65 °C; o (b) - es isocodificante con la secuencia de nucleótidos de (a); o (c) - tiene una identidad de secuencias con la SEQ ID NO: 1, de por lo menos el 93%; o (d) --codifica una secuencia de aminoácidos que tiene una identidad de secuencias con la SEQ ID NO: 2, de por lo menos el 91%.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36225002P | 2002-03-06 | 2002-03-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR038725A1 true AR038725A1 (es) | 2005-01-26 |
Family
ID=27805151
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP030100713A AR038725A1 (es) | 2002-03-06 | 2003-03-04 | Novedosas toxinas vip3, procedentes de bacillus thurigiensis y metodos para su utilizacion |
| ARP090102302A AR072217A2 (es) | 2002-03-06 | 2009-06-23 | Toxina hibrida procedente de bacillusthuringiensis, metodo para controlar insectos, y molecula de acido nucleico |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP090102302A AR072217A2 (es) | 2002-03-06 | 2009-06-23 | Toxina hibrida procedente de bacillusthuringiensis, metodo para controlar insectos, y molecula de acido nucleico |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US7378493B2 (es) |
| EP (1) | EP1499176B1 (es) |
| JP (1) | JP2006500908A (es) |
| CN (1) | CN100473724C (es) |
| AR (2) | AR038725A1 (es) |
| AT (1) | ATE465628T1 (es) |
| AU (2) | AU2003219779B2 (es) |
| BR (1) | BRPI0308104A2 (es) |
| CA (1) | CA2477975C (es) |
| DE (1) | DE60332337D1 (es) |
| ES (1) | ES2344691T3 (es) |
| WO (1) | WO2003075655A2 (es) |
| ZA (1) | ZA200406879B (es) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE465628T1 (de) * | 2002-03-06 | 2010-05-15 | Syngenta Participations Ag | Neue vip3-toxine und ihre verwendung |
| AU2003209748B2 (en) * | 2002-03-22 | 2009-09-10 | Bayer Cropscience Nv | Novel bacillus thuringiensis insecticidal proteins |
| EP2360179A1 (en) * | 2002-03-22 | 2011-08-24 | Bayer BioScience N.V. | Novel bacillus thuringiensis insecticidal proteins |
| GB0225129D0 (en) | 2002-10-29 | 2002-12-11 | Syngenta Participations Ag | Improvements in or relating to organic compounds |
| WO2005054479A1 (en) | 2003-12-01 | 2005-06-16 | Syngenta Participations Ag | Insect resistant cotton plants and methods of detecting the same |
| WO2005054480A2 (en) * | 2003-12-01 | 2005-06-16 | Syngenta Participations Ag | Insect resistant cotton plants and methods of detecting the same |
| WO2005103301A2 (en) * | 2004-03-25 | 2005-11-03 | Syngenta Participations Ag | Corn event mir604 |
| US8140581B2 (en) * | 2008-05-15 | 2012-03-20 | Microsoft Corporation | Configurable view on data models |
| CN102076709B (zh) * | 2008-07-02 | 2014-10-15 | 阿森尼克斯公司 | Axmi-il5、axmi-113、axmi-005、axmi-163和axmi-184：vip3a杀虫蛋白及其使用方法 |
| CA2751724A1 (en) | 2009-02-19 | 2010-08-26 | Pioneer Hi-Bred International, Inc. | Blended refuge deployment via manipulation during hybrid seed production |
| CN103635579B (zh) | 2011-04-05 | 2019-04-23 | 阿森尼克斯公司 | Axmi115变体灭虫基因及其使用方法 |
| WO2013004668A1 (en) | 2011-07-05 | 2013-01-10 | Syngenta Participations Ag | Root preferred promoters derived from rice and methods of use |
| US9861105B2 (en) | 2011-07-28 | 2018-01-09 | Syngenta Participations Ag | Methods and compositions for controlling nematode pests |
| BR112014020164B1 (pt) * | 2012-02-16 | 2022-09-13 | Syngenta Participations Ag | Polipeptídeo pesticida vip3 manipulado, molécula de ácido nucleico, vetor e composição |
| CA2866239C (en) * | 2012-03-08 | 2020-07-28 | Athenix Corp. | Bacillus thuringiensis toxin gene axmi335 and methods for its use |
| ES2682351T3 (es) * | 2013-02-13 | 2018-09-20 | Athenix Corp. | Uso de AXMI184 para el control de los insectos del gusano de la raíz |
| CN103421099A (zh) * | 2013-08-15 | 2013-12-04 | 浙江大学 | 苏云金芽孢杆菌营养期杀虫蛋白Vip3AfAa及其编码基因和应用 |
| AR097995A1 (es) | 2013-10-14 | 2016-04-27 | Syngenta Participations Ag | Método para sembrar filas de cultivos |
| CN104651377A (zh) * | 2013-11-25 | 2015-05-27 | 浙江大学 | 新型的杀虫蛋白 |
| KR20170020760A (ko) * | 2014-06-20 | 2017-02-24 | 다우 아그로사이언시즈 엘엘씨 | 곤충 해충의 방제에 유용한 영양 살곤충 단백질 |
| WO2016033265A1 (en) | 2014-08-29 | 2016-03-03 | Syngenta Participations Ag | Modified vip3 polypeptides |
| CN104845983B (zh) * | 2015-06-05 | 2018-08-17 | 东北农业大学 | 苏云金芽孢杆菌vip3A-6基因、表达蛋白及其应用 |
| CN104845984B (zh) * | 2015-06-05 | 2018-08-17 | 东北农业大学 | 苏云金芽胞杆菌表达蛋白对甜菜夜蛾有高毒力的vip3A-1基因 |
| UA126328C2 (uk) * | 2015-08-27 | 2022-09-21 | Монсанто Текнолоджі Елелсі | Молекула рекомбінантної нуклеїнової кислоти, яка кодує білок, що проявляє інгібуючу активність відносно комах |
| US10743535B2 (en) | 2017-08-18 | 2020-08-18 | H&K Solutions Llc | Insecticide for flight-capable pests |
| CA3077180A1 (en) * | 2017-09-26 | 2019-04-04 | Dow Agrosciences Llc | Chimeric insecticidal proteins |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA48104C2 (uk) * | 1991-10-04 | 2002-08-15 | Новартіс Аг | Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника |
| US6291156B1 (en) | 1997-04-03 | 2001-09-18 | Syngenta Participations Ag | Plant pest control |
| US5877012A (en) * | 1993-03-25 | 1999-03-02 | Novartis Finance Corporation | Class of proteins for the control of plant pests |
| GB9611777D0 (en) | 1996-06-06 | 1996-08-07 | Ciba Geigy Ag | Method of controlling insect pests |
| US6369213B1 (en) * | 1996-07-01 | 2002-04-09 | Mycogen Corporation | Toxins active against pests |
| WO1998000546A2 (en) | 1996-07-01 | 1998-01-08 | Mycogen Corporation | Bacillus thuringiensis toxins active against noctuidae pests |
| US6242669B1 (en) | 1996-10-30 | 2001-06-05 | Mycogen Corporation | Pesticidal toxins and nucleotide sequences which encode these toxins |
| JP2001502919A (ja) | 1996-10-30 | 2001-03-06 | マイコーゲン コーポレーション | 新規殺虫性毒素およびこれらの毒素をコードする塩基配列 |
| US6174860B1 (en) * | 1999-04-16 | 2001-01-16 | Novartis Ag | Insecticidal toxins and nucleic acid sequences coding therefor |
| CA2325567A1 (en) * | 1998-05-01 | 1999-11-11 | Maxygen, Inc. | Optimization of pest resistance genes using dna shuffling |
| AR035799A1 (es) * | 2001-03-30 | 2004-07-14 | Syngenta Participations Ag | Toxinas insecticidas aisladas de bacillus thuringiensis y sus usos. |
| ATE465628T1 (de) * | 2002-03-06 | 2010-05-15 | Syngenta Participations Ag | Neue vip3-toxine und ihre verwendung |
-
2003
- 2003-02-20 AT AT03716053T patent/ATE465628T1/de not_active IP Right Cessation
- 2003-02-20 CN CNB038088975A patent/CN100473724C/zh not_active Expired - Fee Related
- 2003-02-20 BR BRPI0308104A patent/BRPI0308104A2/pt not_active IP Right Cessation
- 2003-02-20 ES ES03716053T patent/ES2344691T3/es not_active Expired - Lifetime
- 2003-02-20 AU AU2003219779A patent/AU2003219779B2/en not_active Ceased
- 2003-02-20 JP JP2003573942A patent/JP2006500908A/ja not_active Withdrawn
- 2003-02-20 US US10/505,315 patent/US7378493B2/en not_active Expired - Fee Related
- 2003-02-20 DE DE60332337T patent/DE60332337D1/de not_active Expired - Lifetime
- 2003-02-20 CA CA2477975A patent/CA2477975C/en not_active Expired - Fee Related
- 2003-02-20 EP EP03716053A patent/EP1499176B1/en not_active Revoked
- 2003-02-20 WO PCT/US2003/004735 patent/WO2003075655A2/en active Application Filing
- 2003-03-04 AR ARP030100713A patent/AR038725A1/es not_active Application Discontinuation
-
2004
- 2004-08-30 ZA ZA200406879A patent/ZA200406879B/en unknown
-
2008
- 2008-04-21 US US12/106,847 patent/US8686232B2/en not_active Expired - Fee Related
- 2008-09-11 AU AU2008216967A patent/AU2008216967B2/en not_active Ceased
-
2009
- 2009-06-23 AR ARP090102302A patent/AR072217A2/es unknown
-
2014
- 2014-02-10 US US14/176,612 patent/US9347072B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ZA200406879B (en) | 2006-12-27 |
| EP1499176A2 (en) | 2005-01-26 |
| US8686232B2 (en) | 2014-04-01 |
| US20140212394A1 (en) | 2014-07-31 |
| ATE465628T1 (de) | 2010-05-15 |
| JP2006500908A (ja) | 2006-01-12 |
| US20090328254A1 (en) | 2009-12-31 |
| CA2477975C (en) | 2013-01-29 |
| WO2003075655A2 (en) | 2003-09-18 |
| DE60332337D1 (de) | 2010-06-10 |
| BRPI0308104A2 (pt) | 2016-06-28 |
| EP1499176A4 (en) | 2006-05-03 |
| US20050210545A1 (en) | 2005-09-22 |
| AR072217A2 (es) | 2010-08-11 |
| AU2003219779A1 (en) | 2003-09-22 |
| US9347072B2 (en) | 2016-05-24 |
| CA2477975A1 (en) | 2003-09-18 |
| CN100473724C (zh) | 2009-04-01 |
| ES2344691T3 (es) | 2010-09-03 |
| AU2008216967B2 (en) | 2012-02-09 |
| US7378493B2 (en) | 2008-05-27 |
| AU2008216967A1 (en) | 2008-10-09 |
| WO2003075655A3 (en) | 2004-05-27 |
| EP1499176B1 (en) | 2010-04-28 |
| CN1646006A (zh) | 2005-07-27 |
| AU2003219779B2 (en) | 2008-06-12 |
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| FC | Refusal |