AR043207A1 - 5-enolpiruvilshikimato-3-fosfato sintetasa (epsps) clase i resistente a glifosato - Google Patents

5-enolpiruvilshikimato-3-fosfato sintetasa (epsps) clase i resistente a glifosato

Info

Publication number
AR043207A1
AR043207A1 ARP040100492A ARP040100492A AR043207A1 AR 043207 A1 AR043207 A1 AR 043207A1 AR P040100492 A ARP040100492 A AR P040100492A AR P040100492 A ARP040100492 A AR P040100492A AR 043207 A1 AR043207 A1 AR 043207A1
Authority
AR
Argentina
Prior art keywords
glyphosate
class
epsps
resistant
isoleucine
Prior art date
Application number
ARP040100492A
Other languages
English (en)
Inventor
Murtaza Alibhai
Claire Cajacob
Youlin Qi
Gregory R Heck
Paul Chi Chia Feng
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR043207A1 publication Critical patent/AR043207A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Composiciones y métodos que proporcionan nuevas moléculas de ADN que codifican proteínas EPSPS resistentes a glifosato, y plantas que contienen estas nuevas proteínas. Las plantas que expresan nuevas proteínas EPSPS presentan tolerancia a los efectos herbicidas del glifosato. Reivindicación 1: Una molécula de ADN aislada que codifica una proteína EPSPS clase I resistente a glifosato, caracterizada porque dicha proteína EPSPS clase I resistente a glifosato comprende una secuencia polipeptídica GX4X1X2RX3, donde X1 y X2 son cualquier aminoácido, X4 es isoleucina o leucina, y X3 se selecciona del grupo que consiste en treonina, glicina, cisteina, alanina e isoleucina. Reivindicación 15: Un método para controlar malezas en un campo de plantas de cultivo con tolerancia a glifosato, caracterizado porque comprende aplicar a dicho campo de plantas de cultivo con tolerancia a glifosato una dosis efectiva de un herbicida que contiene glifosato, donde dichas plantas de cultivo con tolerancia a glifosato contienen una construcción e ADN que comprende un promotor que funciona en células vegetales, unido operativamente a una molécula de ADN que codifica un péptido de tránsito a cloroplasto, unida operativamente a una proteína EPSPS resistente a glifosato clase I, donde dicha proteína EPSP resistente a glifosato clase I comprende una secuencia polipeptídica GIX1X2RX3, donde X1 y X2 son cualquier aminoácido, y X3 se selecciona del grupo que consiste en treonina, glicina, cisteina, alanina e isoleucina.
ARP040100492A 2003-02-18 2004-02-18 5-enolpiruvilshikimato-3-fosfato sintetasa (epsps) clase i resistente a glifosato AR043207A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US44843803P 2003-02-18 2003-02-18

Publications (1)

Publication Number Publication Date
AR043207A1 true AR043207A1 (es) 2005-07-20

Family

ID=32908590

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP040100492A AR043207A1 (es) 2003-02-18 2004-02-18 5-enolpiruvilshikimato-3-fosfato sintetasa (epsps) clase i resistente a glifosato
ARP120103136A AR087673A2 (es) 2003-02-18 2012-08-24 Planta y progenie con tolerancia al glifosato

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP120103136A AR087673A2 (es) 2003-02-18 2012-08-24 Planta y progenie con tolerancia al glifosato

Country Status (10)

Country Link
US (2) US7723575B2 (es)
EP (1) EP1594961B1 (es)
CN (2) CN101173273B (es)
AR (2) AR043207A1 (es)
AU (1) AU2004213818B2 (es)
BR (1) BRPI0407592B1 (es)
CA (1) CA2516221C (es)
MX (1) MXPA05008725A (es)
WO (1) WO2004074443A2 (es)
ZA (1) ZA200506582B (es)

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AU2004213818B2 (en) 2008-06-05
US20100197499A1 (en) 2010-08-05
CN101173273A (zh) 2008-05-07
WO2004074443A3 (en) 2004-12-16
MXPA05008725A (es) 2005-09-20
US7723575B2 (en) 2010-05-25
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