ZA9610384B - Process for the preparing recombinant protein in e coll by means of high cell density fermentation - Google Patents

Process for the preparing recombinant protein in e coll by means of high cell density fermentation

Info

Publication number
ZA9610384B
ZA9610384B ZA9610384A ZA9610384A ZA9610384B ZA 9610384 B ZA9610384 B ZA 9610384B ZA 9610384 A ZA9610384 A ZA 9610384A ZA 9610384 A ZA9610384 A ZA 9610384A ZA 9610384 B ZA9610384 B ZA 9610384B
Authority
ZA
South Africa
Prior art keywords
coll
recombinant protein
cell density
high cell
density fermentation
Prior art date
Application number
ZA9610384A
Other languages
English (en)
Inventor
Wolfgang Strittmatter
Siegfried Matzku
Dieter Riesenberg
Uwe Horn
Uwe Knuepfer
Marian Kujau
Rolf Wenderoth
Andreas Plueckthun
Anke Krebber
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Publication of ZA9610384B publication Critical patent/ZA9610384B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
ZA9610384A 1995-12-11 1996-12-10 Process for the preparing recombinant protein in e coll by means of high cell density fermentation ZA9610384B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP95119478 1995-12-11

Publications (1)

Publication Number Publication Date
ZA9610384B true ZA9610384B (en) 1997-06-23

Family

ID=8219878

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA9610384A ZA9610384B (en) 1995-12-11 1996-12-10 Process for the preparing recombinant protein in e coll by means of high cell density fermentation

Country Status (24)

Country Link
US (1) US6410270B1 (da)
EP (1) EP0866876B1 (da)
JP (2) JP4101879B2 (da)
KR (1) KR100444735B1 (da)
CN (1) CN1117160C (da)
AR (1) AR005035A1 (da)
AT (1) ATE213271T1 (da)
AU (1) AU716612B2 (da)
BR (1) BR9611937A (da)
CA (1) CA2240097C (da)
CZ (1) CZ291415B6 (da)
DE (1) DE59608743D1 (da)
DK (1) DK0866876T3 (da)
ES (1) ES2171752T3 (da)
HU (1) HU227945B1 (da)
NO (1) NO319090B1 (da)
PL (1) PL185665B1 (da)
PT (1) PT866876E (da)
RU (1) RU2201455C2 (da)
SI (1) SI0866876T1 (da)
SK (1) SK283169B6 (da)
UA (1) UA61066C2 (da)
WO (1) WO1997021829A1 (da)
ZA (1) ZA9610384B (da)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9715895D0 (en) 1997-07-29 1997-10-01 Zeneca Ltd Heterocyclic compounds
US7858339B1 (en) 1998-10-28 2010-12-28 Genentech, Inc. Process for bacterial production of polypeptides
AU3944002A (en) 2000-11-03 2002-05-27 Genentech Inc Metabolic rate shifts in fermentations expressing recombinant proteins
ATE416260T1 (de) * 2001-07-06 2008-12-15 Merck Patent Gmbh Verfahren zur überwachung und modulierung von proteinfaltung
JP2007530013A (ja) * 2003-12-12 2007-11-01 コンジュゴン インコーポレーティッド 緻密に調節された遺伝子発現のためのシステム
DE60332667D1 (de) * 2003-12-23 2010-07-01 Council Scient Ind Res Verfahren zur herstellung von streptokinase durch kultivierung von genetisch veränderten escherichia coli
NZ556635A (en) * 2004-12-22 2010-11-26 Genentech Inc Method for producing soluble multi-membrane-spanning proteins
DK2061897T3 (da) * 2006-07-27 2020-06-02 Wyeth Llc Fed-batch-fermenteringsproces med høj celledensitet til fremstilling af rekombinant protein
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
SG174804A1 (da) 2006-09-13 2011-10-28 Abbott Lab
AU2009290563A1 (en) 2008-09-15 2010-03-18 Genentech, Inc. Compositions and methods for regulating cell osmolarity
CN102471787B (zh) * 2009-07-28 2016-01-20 三井化学株式会社 乳酸制造方法
CA2788607C (en) 2010-02-01 2018-03-20 Digna Biotech,S.L. Process for the production of interferon alpha 5
ES2558751T3 (es) * 2010-08-30 2016-02-08 F. Hoffmann-La Roche Ag Alimento alcalino
KR101252150B1 (ko) * 2010-11-18 2013-04-08 한국과학기술원 재조합 ＤＮＡ 분자 클로닝을 위한 알데히드 환원효소 ＹｑｈＤ 유전자를 포함하는 자살 벡터
RU2507736C2 (ru) * 2011-01-11 2014-02-27 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук (ИБГ РАН) Способ создания трансгенных растений с высоким уровнем экспрессии трансгенного белка
WO2012149197A2 (en) 2011-04-27 2012-11-01 Abbott Laboratories Methods for controlling the galactosylation profile of recombinantly-expressed proteins
RU2496877C2 (ru) * 2011-12-15 2013-10-27 Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") ПЛАЗМИДНЫЙ ВЕКТОР pHYP С ПОВЫШЕННОЙ СЕГРЕГАЦИОННОЙ СТАБИЛЬНОСТЬЮ ДЛЯ ЭКСПРЕССИИ РЕКОМБИНАНТНОГО БЕЛКА, БАКТЕРИЯ - ПРОДУЦЕНТ ПРЕДШЕСТВЕННИКА РЕКОМБИНАНТНОГО БЕЛКА И СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОГО БЕЛКА
EP2817625A2 (en) 2012-02-23 2014-12-31 Stage Cell Therapeutics GmbH Chromatographic isolation of cells and other complex biological materials
DK2825633T3 (da) 2012-03-12 2019-05-20 Hanmi Science Co Ltd Fremgangsmåde til dyrkning af E. coli-celler til høj densitet
CN102604904B (zh) * 2012-03-14 2013-09-18 苏州汉酶生物技术有限公司 一种葡萄糖脱氢酶的生产方法
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
WO2014035475A1 (en) 2012-09-02 2014-03-06 Abbvie Inc. Methods to control protein heterogeneity
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
WO2014137903A2 (en) 2013-03-08 2014-09-12 Genzyme Corporation Integrated continuous manufacturing of therapeutic protein drug substances
EP2830651A4 (en) 2013-03-12 2015-09-02 Abbvie Inc HUMAN ANTIBODIES THAT BIND TNF-ALPHA AND PREPARATION METHODS
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
TWI671312B (zh) 2014-01-17 2019-09-11 美商健臻公司 無菌層析法及製法
WO2016057851A1 (en) * 2014-10-08 2016-04-14 Lewis Randolph V Expression systems and associated methods
MA45489A (fr) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh Procédés de culture de cellules, kits et appareil associés
TW201842335A (zh) 2017-04-27 2018-12-01 德商朱諾醫療公司 寡聚粒子試劑及其使用方法
US11369703B2 (en) 2018-08-31 2022-06-28 Genzyme Corporation Sterile chromatography resin and use thereof in manufacturing processes
CN110684101A (zh) * 2019-11-15 2020-01-14 上海松皓生物科技有限公司 一种重组水蛭素的制备方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ282603B6 (cs) * 1991-03-06 1997-08-13 Merck Patent Gesellschaft Mit Beschränkter Haftun G Humanizované a chimerické monoklonální protilátky

Also Published As

Publication number Publication date
SK74098A3 (en) 1998-11-04
AR005035A1 (es) 1999-04-07
WO1997021829A1 (de) 1997-06-19
HUP0000317A2 (hu) 2000-06-28
HUP0000317A3 (en) 2001-09-28
JP2000501936A (ja) 2000-02-22
HU227945B1 (en) 2012-06-28
NO982672D0 (no) 1998-06-10
PT866876E (pt) 2002-07-31
AU1032597A (en) 1997-07-03
KR100444735B1 (ko) 2004-11-10
PL327188A1 (en) 1998-11-23
JP2007185197A (ja) 2007-07-26
DE59608743D1 (de) 2002-03-21
DK0866876T3 (da) 2002-05-27
EP0866876A1 (de) 1998-09-30
CA2240097A1 (en) 1997-06-19
JP4102422B2 (ja) 2008-06-18
UA61066C2 (en) 2003-11-17
PL185665B1 (pl) 2003-06-30
JP4101879B2 (ja) 2008-06-18
EP0866876B1 (de) 2002-02-13
BR9611937A (pt) 1999-03-02
KR19990072037A (ko) 1999-09-27
CZ291415B6 (cs) 2003-03-12
US6410270B1 (en) 2002-06-25
NO319090B1 (no) 2005-06-20
SI0866876T1 (en) 2002-08-31
CN1117160C (zh) 2003-08-06
ATE213271T1 (de) 2002-02-15
ES2171752T3 (es) 2002-09-16
CZ165298A3 (cs) 1998-08-12
NO982672L (no) 1998-06-10
AU716612B2 (en) 2000-03-02
CA2240097C (en) 2005-07-26
CN1219203A (zh) 1999-06-09
SK283169B6 (sk) 2003-03-04
RU2201455C2 (ru) 2003-03-27

Similar Documents

Publication Publication Date Title
ZA9610384B (en) Process for the preparing recombinant protein in e coll by means of high cell density fermentation
HUP9800901A3 (en) Process, medium and cell culture system for the preparation of recombinant factor viii in a protein free medium
IL158303A0 (en) Method for producing protein in an immortalized cell
ZA964776B (en) Mammalian cell culture process.
EP0483085A3 (en) Process of preparing electrode for fuel cell
AU650909B2 (en) Process for introducing foreign DNA into cell
EP0411658A3 (en) Method for culturing cells
IL96347A (en) Process for the preparation of genetic vectors for the nerve growth factor expression in eukaryotic cells
EP1143009A4 (en) PROCESS FOR PRODUCING POLYPEPTIDE IN A CELLULAR PROTEIN SYNTHESIS SYSTEM
EP0816510A4 (en) PROCESS FOR PRODUCING BIOLOGICALLY ACTIVE FUSED PROTEINS
HUP0001837A3 (en) Novel dnas and process for producing proteins by using the same
IL96143A0 (en) Methods for the production of proteins and polypeptides and for enhancement of cell growth
HUT58817A (en) Process for producing dns-constructs for expressing proteins in milk-glands of transgen mammalian animals
EP0795027A4 (en) IN VITRO SYNTHESIS OF PROTEINS
EP0841991A4 (en) PROCESS FOR THE PREPARATION OF MEMBRANE FOR CHLORINE-ALCALI CELLS
EP0870828A4 (en) NOVEL POLYPEPTIDETIRE OF HEMOPHILUS PARAGALLINARUM AND PROCESS FOR PRODUCING THE SAME
EP0736605A4 (en) Process for the production of protein
GB2314332B (en) Gene expression in mammalian cells
HU9403070D0 (en) Improved method for recombinant protein production
AU4365196A (en) Purified genes encoding mammalian cell surface antigens; proteins and antibodies
GB2241505B (en) Fermentation process with improved accumulation of protein
NZ304538A (en) Process for producing a modified recombinant protein in a host cell comprising preparing a single vector encoding both the exogenous protein and an enzyme capable of modifying the exogenous protein
LTIP1543A (en) Process for enzymatic decomposition of recombinant proteins by means of iga-protease, fused protein, recombinant dna, cell
AU5276196A (en) Method for the continuous preparation of recombinant proteinsfrom cell cultures
EP0460674A3 (en) Recombinant dna and process for the production of chimaeric antibodies