ZA201405858B - Recombinant microorganisms and methods of use thereof - Google Patents

Recombinant microorganisms and methods of use thereof

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Publication number
ZA201405858B
ZA201405858B ZA2014/05858A ZA201405858A ZA201405858B ZA 201405858 B ZA201405858 B ZA 201405858B ZA 2014/05858 A ZA2014/05858 A ZA 2014/05858A ZA 201405858 A ZA201405858 A ZA 201405858A ZA 201405858 B ZA201405858 B ZA 201405858B
Authority
ZA
South Africa
Prior art keywords
methods
recombinant microorganisms
microorganisms
recombinant
Prior art date
Application number
ZA2014/05858A
Other languages
English (en)
Inventor
Chen Wendy
Nagaraju Shilpa
Koepke Michael
Original Assignee
Lanzatech New Zealand Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lanzatech New Zealand Ltd filed Critical Lanzatech New Zealand Ltd
Publication of ZA201405858B publication Critical patent/ZA201405858B/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1022Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/06Alanine; Leucine; Isoleucine; Serine; Homoserine
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/08Lysine; Diaminopimelic acid; Threonine; Valine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/065Ethanol, i.e. non-beverage with microorganisms other than yeasts
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    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42Hydroxy-carboxylic acids
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    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44Polycarboxylic acids
    • C12P7/46Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
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    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44Polycarboxylic acids
    • C12P7/48Tricarboxylic acids, e.g. citric acid
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    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44Polycarboxylic acids
    • C12P7/50Polycarboxylic acids having keto groups, e.g. 2-ketoglutaric acid
    • CCHEMISTRY; METALLURGY
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    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56Lactic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01004R,R-butanediol dehydrogenase (1.1.1.4)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y202/00Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01Transketolases and transaldolases (2.2.1)
    • C12Y202/01006Acetolactate synthase (2.2.1.6)
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y401/00Carbon-carbon lyases (4.1)
    • C12Y401/01Carboxy-lyases (4.1.1)
    • C12Y401/01005Acetolactate decarboxylase (4.1.1.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
ZA2014/05858A 2012-01-31 2014-08-11 Recombinant microorganisms and methods of use thereof ZA201405858B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261593269P 2012-01-31 2012-01-31
PCT/NZ2013/000012 WO2013115659A2 (en) 2012-01-31 2013-01-31 Recombinant microorganisms and methods of use thereof

Publications (1)

Publication Number Publication Date
ZA201405858B true ZA201405858B (en) 2016-08-31

Family

ID=48905994

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA2014/05858A ZA201405858B (en) 2012-01-31 2014-08-11 Recombinant microorganisms and methods of use thereof

Country Status (13)

Country Link
US (2) US9057071B2 (enExample)
EP (1) EP2710117B1 (enExample)
JP (2) JP6512825B2 (enExample)
KR (1) KR101511639B1 (enExample)
CN (1) CN104395455B (enExample)
AU (1) AU2013215706B2 (enExample)
BR (1) BR112014018844B1 (enExample)
CA (1) CA2862790C (enExample)
EA (1) EA031093B1 (enExample)
ES (1) ES2583203T3 (enExample)
MY (1) MY165103A (enExample)
WO (1) WO2013115659A2 (enExample)
ZA (1) ZA201405858B (enExample)

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KR101511639B1 (ko) * 2012-01-31 2015-04-16 란자테크 뉴질랜드 리미티드 재조합 미생물 및 이의 사용 방법
CN112048525B (zh) * 2013-09-22 2024-03-12 朗泽科技新西兰有限公司 发酵工艺
WO2015085015A1 (en) * 2013-12-03 2015-06-11 Lanzatech New Zealand Limited Microorganisms and methods for the production of ketones
EP3099783A4 (en) * 2014-01-30 2017-09-20 Lanzatech New Zealand Limited Recombinant microorganisms and methods of use thereof
MY178182A (en) * 2014-08-11 2020-10-06 Lanzatech New Zealand Ltd Genetically engineered bacterium with altered carbon monoxide dehydrogenase (codh) activity
HUE052810T2 (hu) * 2014-12-08 2021-05-28 Lanzatech New Zealand Ltd Rekombináns mikroorganizmusok, amelyek megnövekedett fluxust mutatnak fermetációs útvonalon keresztül
CA2995859A1 (en) * 2015-09-02 2017-03-09 Sekisui Chemical Co., Ltd. Recombinant cells, method for producing recombinant cells, and method for producing 1,4-butanediol
MY205861A (en) 2015-10-13 2024-11-16 Lanzatech Nz Inc Genetically engineered bacterium comprising energy-generating fermentation pathway
WO2017119731A1 (ko) * 2016-01-06 2017-07-13 주식회사 씨원켐 Co 수화효소 및 이를 이용한 개미산의 제조방법
US11162115B2 (en) 2017-06-30 2021-11-02 Inv Nylon Chemicals Americas, Llc Methods, synthetic hosts and reagents for the biosynthesis of hydrocarbons
US11634733B2 (en) 2017-06-30 2023-04-25 Inv Nylon Chemicals Americas, Llc Methods, materials, synthetic hosts and reagents for the biosynthesis of hydrocarbons and derivatives thereof
US11505809B2 (en) * 2017-09-28 2022-11-22 Inv Nylon Chemicals Americas Llc Organisms and biosynthetic processes for hydrocarbon synthesis
WO2019147743A1 (en) 2018-01-26 2019-08-01 Massachusetts Institute Of Technology Structure-guided chemical modification of guide rna and its applications
CA3131243A1 (en) * 2019-02-25 2020-09-03 Northwestern University Cell-free protein synthesis platforms derived from clostridia
CN111089914A (zh) * 2019-11-07 2020-05-01 河北首朗新能源科技有限公司 微生物发酵生产乙醇中代谢物的高效液相检测方法
CN111154683B (zh) * 2020-01-19 2022-05-13 南京工业大学 一种食甲基丁酸杆菌的优化培养方法及其应用
CN111220762A (zh) * 2020-03-24 2020-06-02 河北首朗新能源科技有限公司 一种乙醇梭菌蛋白中有机物的含量的检测方法及应用
US12134794B2 (en) * 2020-04-29 2024-11-05 Lanzatech, Inc. Fermentative production of B-ketoadipate from gaseous substrates
KR20230156819A (ko) * 2020-06-06 2023-11-14 란자테크, 인크. 아세토락테이트 탈카복실화효소 유전자위에 녹인이 있는 미생물
JP2023542338A (ja) * 2020-09-25 2023-10-06 ランザテク,インコーポレイテッド 組換え微生物及びそのための使用
CN113234653B (zh) * 2021-04-22 2022-10-04 中国科学院青岛生物能源与过程研究所 一种提高合成气发酵产乙醇含量的突变株和利用突变株的应用
US20240117393A1 (en) 2022-09-30 2024-04-11 Ajinomoto Co., Inc. Method for producing l-amino acid
CN115960808A (zh) * 2022-11-24 2023-04-14 苏州聚维元创生物科技有限公司 一种敲除基因的重组大肠杆菌及其构建方法

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JP5437631B2 (ja) * 2005-07-01 2014-03-12 ザ ユニバーシティー オブ フロリダ リサーチ ファンデーション インク エタノール産生のための組み換えホスト細胞及び培地
WO2008013996A2 (en) * 2006-07-27 2008-01-31 Gevo Inc. Engineered microorganisms for increasing product yield in biotransformations, related methods and systems
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CN102876609A (zh) * 2007-11-13 2013-01-16 兰扎泰克新西兰有限公司 新型菌及其使用方法
BRPI0909334B1 (pt) * 2008-03-12 2019-10-29 Lanzatech New Zealand Ltd método para a conversão de ácido em álcool correspondente
AU2009258344B2 (en) * 2008-06-09 2013-11-07 Lanzatech Nz, Inc. Production of butanediol by anaerobic microbial fermentation
DE102009002583A1 (de) * 2009-04-23 2010-10-28 Evonik Degussa Gmbh Zellen und Verfahren zur Herstellung von Aceton
US8143037B2 (en) * 2010-03-19 2012-03-27 Coskata, Inc. Ethanologenic Clostridium species, Clostridium coskatii
BR112013003644B1 (pt) * 2010-07-28 2020-11-17 Lanzatech New Zealand Limited isolado biologicamente puro de uma bactéria clostridium autoethanogenum
KR101511639B1 (ko) * 2012-01-31 2015-04-16 란자테크 뉴질랜드 리미티드 재조합 미생물 및 이의 사용 방법

Also Published As

Publication number Publication date
WO2013115659A3 (en) 2013-10-03
MY165103A (en) 2018-02-28
US9057071B2 (en) 2015-06-16
ES2583203T3 (es) 2016-09-19
KR20130138866A (ko) 2013-12-19
WO2013115659A2 (en) 2013-08-08
EA201491418A1 (ru) 2015-02-27
CA2862790C (en) 2016-09-27
US20150299737A1 (en) 2015-10-22
JP2019088292A (ja) 2019-06-13
AU2013215706B2 (en) 2014-03-27
BR112014018844B1 (pt) 2022-09-27
BR112014018844A2 (pt) 2020-08-18
KR101511639B1 (ko) 2015-04-16
JP6898365B2 (ja) 2021-07-07
AU2013215706A1 (en) 2013-10-10
CN104395455B (zh) 2020-05-19
EA031093B1 (ru) 2018-11-30
EP2710117A2 (en) 2014-03-26
JP6512825B2 (ja) 2019-05-15
US20140206901A1 (en) 2014-07-24
EP2710117A4 (en) 2014-04-16
US9297026B2 (en) 2016-03-29
EP2710117B1 (en) 2016-04-27
JP2015505471A (ja) 2015-02-23
CA2862790A1 (en) 2013-08-08
CN104395455A (zh) 2015-03-04

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