YU1502A - Postupci i smeše za usmereno kloniranje i subkloniranje primenom homologne rekombinacije - Google Patents
Postupci i smeše za usmereno kloniranje i subkloniranje primenom homologne rekombinacijeInfo
- Publication number
- YU1502A YU1502A YU1502A YUP1502A YU1502A YU 1502 A YU1502 A YU 1502A YU 1502 A YU1502 A YU 1502A YU P1502 A YUP1502 A YU P1502A YU 1502 A YU1502 A YU 1502A
- Authority
- YU
- Yugoslavia
- Prior art keywords
- compositions
- methods
- homologous recombination
- subcloning
- cloning
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Production Of Liquid Hydrocarbon Mixture For Refining Petroleum (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Solid Fuels And Fuel-Associated Substances (AREA)
- Detergent Compositions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicinal Preparation (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
Abstract
Predmetni pronalazak je usmeren na postupke i kompozicije za subkloniranje DNK primenom homologne rekombinacije posredovane bakterijskom rekombinazom. Pronalazak se odnosi na postupke kloniranja, kompozicije koje sadrže polinukleotide koji se mogu koristiti kao klonski vektori, ćelije koje sadrže ovakve polinukleotidne kompozicije i komplete za kloniranje posredovano bakterijskom rekombinazom, kao što su RecE/T i Redα/Гџ.[The present invention is directed to methods and compositions for DNA subcloning using bacterial recombinase-mediated homologous recombination. The invention relates to methods for cloning, compositions comprising polynucleotides useful as cloning vectors, cells comprising such polynucleotide compositions, and kits useful for cloning mediated by bacterial recombinases, such as RecE/T and Redα/Гџ.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/350,830 US6355412B1 (en) | 1999-07-09 | 1999-07-09 | Methods and compositions for directed cloning and subcloning using homologous recombination |
Publications (1)
Publication Number | Publication Date |
---|---|
YU1502A true YU1502A (sh) | 2005-11-28 |
Family
ID=23378374
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
YU1502A YU1502A (sh) | 1999-07-09 | 2000-07-10 | Postupci i smeše za usmereno kloniranje i subkloniranje primenom homologne rekombinacije |
Country Status (21)
Country | Link |
---|---|
US (1) | US6355412B1 (sh) |
EP (1) | EP1204740B1 (sh) |
JP (2) | JP4312981B2 (sh) |
KR (1) | KR100751587B1 (sh) |
CN (2) | CN101492694B (sh) |
AT (1) | ATE305035T1 (sh) |
AU (1) | AU781379B2 (sh) |
BR (1) | BR0012283A (sh) |
CA (1) | CA2377938C (sh) |
DE (1) | DE60022766T2 (sh) |
DK (1) | DK1204740T3 (sh) |
ES (1) | ES2249291T3 (sh) |
HK (1) | HK1046926A1 (sh) |
HU (1) | HU228977B1 (sh) |
IL (1) | IL147385A0 (sh) |
MX (1) | MX252928B (sh) |
NZ (1) | NZ516722A (sh) |
PL (1) | PL353634A1 (sh) |
WO (1) | WO2001004288A1 (sh) |
YU (1) | YU1502A (sh) |
ZA (1) | ZA200200152B (sh) |
Families Citing this family (85)
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JP4303418B2 (ja) * | 1997-10-24 | 2009-07-29 | ライフ テクノロジーズ コーポレーション | 組換え部位を有する核酸を使用する組換えクローニング |
US6355412B1 (en) * | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
AU2001283377B2 (en) * | 2000-08-14 | 2007-09-13 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Enhanced homologous recombination mediated by lambda recombination proteins |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US6596541B2 (en) | 2000-10-31 | 2003-07-22 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
US20050144655A1 (en) | 2000-10-31 | 2005-06-30 | Economides Aris N. | Methods of modifying eukaryotic cells |
US7083976B2 (en) * | 2001-06-30 | 2006-08-01 | The European Molecular Biology Laboratory | Tyrosine recombinase for genetic engineering |
WO2003010322A1 (en) * | 2001-07-20 | 2003-02-06 | Youming Zhang | Improved rect or recet cloning and subcloning method |
AU2003217222A1 (en) * | 2002-01-16 | 2003-09-02 | Baylor College Of Medicine | In vivo gene transfer |
EP1474522B1 (en) * | 2002-01-18 | 2012-03-07 | Morphotek, Inc. | A method for generating engineered cells for locus specific gene regulation and analysis |
JP2005523014A (ja) * | 2002-04-16 | 2005-08-04 | プロメガ コーポレイション | 相同的組換えの増強方法 |
US20040106566A1 (en) * | 2002-05-17 | 2004-06-03 | Shi-Lung Lin | RNA-splicing and processing-directed gene silencing and the relative applications thereof |
WO2004013299A2 (en) * | 2002-08-02 | 2004-02-12 | The General Hospital Corporation | Methods for the production of cells and mammals with desired genetic modifications |
US7135324B2 (en) * | 2002-09-04 | 2006-11-14 | The University Of Connecticut | Viral recombinases, related articles, and methods of use thereof |
US7563600B2 (en) * | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
EP1475437A4 (en) * | 2002-12-10 | 2005-06-01 | Kazusa Dna Res Inst Foundation | METHOD FOR EFFICIENT RECOMBINATION OF ORF CODE BY CDC AND MODEL VECTOR, AND TRAPPER VECTOR AND PRIMER FOR USE THEREIN |
US20040209370A1 (en) * | 2002-12-19 | 2004-10-21 | Wonchul Suh | Method for chromosomal engineering |
US7468269B2 (en) * | 2003-02-26 | 2008-12-23 | University Of Massachusetts | Reagents for recombinogenic engineering and uses thereof |
US7521242B2 (en) * | 2003-05-09 | 2009-04-21 | The United States Of America As Represented By The Department Of Health And Human Services | Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids |
US20100069614A1 (en) | 2008-06-27 | 2010-03-18 | Merus B.V. | Antibody producing non-human mammals |
CA2527694C (en) | 2003-05-30 | 2015-07-14 | Hendricus Renerus Jacobus Mattheus Hoogenboom | Fab library for the preparation of anti vegf and anti rabies virus fabs |
US7781190B2 (en) * | 2003-07-24 | 2010-08-24 | The University Of Hong Kong | Method for constructing and modifying large DNA molecules |
EP1687323A4 (en) | 2003-08-08 | 2009-08-12 | Life Technologies Corp | METHODS AND COMPOSITIONS FOR DIRECT CLONING OF NUCLEIC ACID MOLECULES |
EP1697534B1 (en) * | 2003-12-01 | 2010-06-02 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
CA2558749A1 (en) * | 2004-02-27 | 2005-09-29 | President And Fellows Of Harvard College | Polynucleotide synthesis |
US7674621B2 (en) * | 2004-05-21 | 2010-03-09 | The United States Of America As Represented By The Department Of Health And Human Services | Plasmids and phages for homologous recombination and methods of use |
GB0423755D0 (en) * | 2004-10-26 | 2004-11-24 | Gene Bridges Gmbh | Methods for heterologus expression of secondary metabolites |
US20080051326A1 (en) * | 2004-11-12 | 2008-02-28 | Alexander Dylan C | Antiinfective Lipopeptides |
WO2006127423A2 (en) * | 2005-05-18 | 2006-11-30 | Codon Devices, Inc. | Methods of producing polynucleotide libraries using scarless ligation |
WO2007005053A1 (en) * | 2005-06-30 | 2007-01-11 | Codon Devices, Inc. | Hierarchical assembly methods for genome engineering |
WO2007136834A2 (en) * | 2006-05-19 | 2007-11-29 | Codon Devices, Inc. | Combined extension and ligation for nucleic acid assembly |
WO2008027558A2 (en) | 2006-08-31 | 2008-03-06 | Codon Devices, Inc. | Iterative nucleic acid assembly using activation of vector-encoded traits |
WO2008067744A1 (fr) * | 2006-12-07 | 2008-06-12 | Huawei Technologies Co., Ltd. | Procédé, système et serveur de filtrage pour filtrer le contenu de communication d'un utilisateur itinérant |
CN101016551B (zh) * | 2007-02-01 | 2010-05-19 | 南京师范大学 | 一种在dna载体中同时引入多个dna片段的方法 |
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GB0803109D0 (en) * | 2008-02-20 | 2008-03-26 | Gene Bridges Gmbh | Method of nucleic acid recombination |
JP5628664B2 (ja) * | 2008-03-07 | 2014-11-19 | 国立大学法人富山大学 | 相同組換え方法およびクローニング方法並びにキット |
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BR112017021667A2 (pt) | 2015-04-09 | 2018-07-24 | Genomatica Inc | microorganismos modificados & métodos para produção aperfeiçoada de álcool de crotila |
EP3440213A1 (en) * | 2016-04-06 | 2019-02-13 | Gene Bridges GmbH | Adenoviral vectors |
CN106834335B (zh) * | 2017-01-11 | 2020-05-08 | 浙江科技学院 | 在酵母菌中实现高准确率基因同源克隆的方法 |
CN110494566A (zh) | 2017-02-02 | 2019-11-22 | 嘉吉公司 | 产生c6-c10脂肪酸衍生物的经遗传修饰的细胞 |
WO2019046350A1 (en) * | 2017-08-30 | 2019-03-07 | President And Fellows Of Harvard College | ITERATIVE GENOMIC ASSEMBLY |
JP7288915B2 (ja) * | 2018-10-04 | 2023-06-08 | 株式会社カネカ | 植物のゲノム編集に用いられるdna構築物 |
KR102129584B1 (ko) | 2018-11-26 | 2020-07-02 | 충북대학교 산학협력단 | 목적 유전자를 선형 벡터에 삽입하는 방법 |
JP2022530653A (ja) * | 2019-05-01 | 2022-06-30 | パクト ファーマ インコーポレイテッド | Tet2改変t細胞療法を使用するがんの治療のための組成物及び方法 |
CN110184287B (zh) * | 2019-05-24 | 2024-01-30 | 华南农业大学 | 一种制备重组病毒的方法及其应用 |
CN110218724A (zh) * | 2019-06-13 | 2019-09-10 | 山西农业大学 | 猪nr1h3基因过表达载体质粒的构建方法及其表达水平的检测 |
Family Cites Families (4)
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AU4386393A (en) * | 1992-05-21 | 1993-12-13 | Genebiomed, Inc. | Process for gene targeting and genome manipulations |
WO1998059060A1 (en) | 1997-06-23 | 1998-12-30 | The Rockefeller University | Methods of preforming homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof |
JP4139561B2 (ja) | 1997-12-05 | 2008-08-27 | ユーロペーイシェ ラボラトリウム フュール モレキュラーバイオロジー(イーエムビーエル) | 新規dnaクローニング方法 |
US6355412B1 (en) * | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
-
1999
- 1999-07-09 US US09/350,830 patent/US6355412B1/en not_active Expired - Lifetime
-
2000
- 2000-07-10 CA CA2377938A patent/CA2377938C/en not_active Expired - Lifetime
- 2000-07-10 HU HU0201963A patent/HU228977B1/hu not_active IP Right Cessation
- 2000-07-10 KR KR1020027000338A patent/KR100751587B1/ko active IP Right Grant
- 2000-07-10 PL PL00353634A patent/PL353634A1/xx unknown
- 2000-07-10 YU YU1502A patent/YU1502A/sh unknown
- 2000-07-10 CN CN2009100050851A patent/CN101492694B/zh not_active Expired - Fee Related
- 2000-07-10 BR BR0012283-1A patent/BR0012283A/pt not_active Application Discontinuation
- 2000-07-10 JP JP2001509492A patent/JP4312981B2/ja not_active Expired - Fee Related
- 2000-07-10 AU AU66911/00A patent/AU781379B2/en not_active Ceased
- 2000-07-10 AT AT00954461T patent/ATE305035T1/de not_active IP Right Cessation
- 2000-07-10 MX MXPA02000233 patent/MX252928B/es active IP Right Grant
- 2000-07-10 DE DE60022766T patent/DE60022766T2/de not_active Expired - Lifetime
- 2000-07-10 WO PCT/EP2000/006533 patent/WO2001004288A1/en active IP Right Grant
- 2000-07-10 DK DK00954461T patent/DK1204740T3/da active
- 2000-07-10 ES ES00954461T patent/ES2249291T3/es not_active Expired - Lifetime
- 2000-07-10 NZ NZ51672200A patent/NZ516722A/en not_active IP Right Cessation
- 2000-07-10 IL IL14738500A patent/IL147385A0/xx active IP Right Grant
- 2000-07-10 EP EP00954461A patent/EP1204740B1/en not_active Expired - Lifetime
- 2000-07-10 CN CNB008127395A patent/CN100469877C/zh not_active Expired - Fee Related
-
2002
- 2002-01-08 ZA ZA200200152A patent/ZA200200152B/en unknown
- 2002-11-14 HK HK02108259A patent/HK1046926A1/xx not_active IP Right Cessation
-
2008
- 2008-11-28 JP JP2008303506A patent/JP5067970B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
NZ516722A (sh) | 2003-12-19 |
WO2001004288A1 (en) | 2001-01-18 |
JP4312981B2 (ja) | 2009-08-12 |
KR100751587B1 (ko) | 2007-08-28 |
IL147385A0 (en) | 2002-08-14 |
CA2377938A1 (en) | 2001-01-18 |
HU228977B1 (hu) | 2013-07-29 |
ZA200200152B (en) | 2003-01-08 |
CN100469877C (zh) | 2009-03-18 |
DE60022766D1 (de) | 2006-02-02 |
PL353634A1 (en) | 2003-12-01 |
MX252928B (es) | 2008-01-04 |
CN1373803A (zh) | 2002-10-09 |
DK1204740T3 (da) | 2006-02-20 |
ES2249291T3 (es) | 2006-04-01 |
JP2009106295A (ja) | 2009-05-21 |
CN101492694B (zh) | 2011-08-31 |
CA2377938C (en) | 2018-01-09 |
EP1204740B1 (en) | 2005-09-21 |
MXPA02000233A (es) | 2003-08-20 |
CN101492694A (zh) | 2009-07-29 |
BR0012283A (pt) | 2002-06-11 |
JP5067970B2 (ja) | 2012-11-07 |
ATE305035T1 (de) | 2005-10-15 |
US6355412B1 (en) | 2002-03-12 |
EP1204740A1 (en) | 2002-05-15 |
AU6691100A (en) | 2001-01-30 |
DE60022766T2 (de) | 2006-07-06 |
HUP0201963A2 (en) | 2002-09-28 |
HUP0201963A3 (en) | 2004-01-28 |
KR20020033726A (ko) | 2002-05-07 |
JP2003504053A (ja) | 2003-02-04 |
HK1046926A1 (en) | 2003-01-30 |
AU781379B2 (en) | 2005-05-19 |
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