BR112017021667A2 - microorganismos modificados & métodos para produção aperfeiçoada de álcool de crotila - Google Patents

microorganismos modificados & métodos para produção aperfeiçoada de álcool de crotila

Info

Publication number
BR112017021667A2
BR112017021667A2 BR112017021667A BR112017021667A BR112017021667A2 BR 112017021667 A2 BR112017021667 A2 BR 112017021667A2 BR 112017021667 A BR112017021667 A BR 112017021667A BR 112017021667 A BR112017021667 A BR 112017021667A BR 112017021667 A2 BR112017021667 A2 BR 112017021667A2
Authority
BR
Brazil
Prior art keywords
improved
crotyl alcohol
methods
alcohol production
modified microorganisms
Prior art date
Application number
BR112017021667A
Other languages
English (en)
Inventor
C Ralph Erik
Gregory Hoff Kevin
Eakley Nicholas
Pharkya Priti
E Osterhout Robin
J Culler Stephanie
Van Dien Stephen
Original Assignee
Genomatica Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomatica Inc filed Critical Genomatica Inc
Publication of BR112017021667A2 publication Critical patent/BR112017021667A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

são revelados métodos e microrganismos modificados que aumentam ou melhoram a produção de álcool de crotila. os microrganismos modificados incluem modificações genéticas em desidrogenase de álcool, redutase de alqueno ou ambas as atividades enzimáticas. por tais modificações genéticas, uma via de produção de álcool de crotila é fornecida ou aperfeiçoada.
BR112017021667A 2015-04-09 2016-04-07 microorganismos modificados & métodos para produção aperfeiçoada de álcool de crotila BR112017021667A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562145290P 2015-04-09 2015-04-09
PCT/US2016/026445 WO2016164586A1 (en) 2015-04-09 2016-04-07 Engineered microorganisms & methods for improved crotyl alcohol production

Publications (1)

Publication Number Publication Date
BR112017021667A2 true BR112017021667A2 (pt) 2018-07-24

Family

ID=55854788

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017021667A BR112017021667A2 (pt) 2015-04-09 2016-04-07 microorganismos modificados & métodos para produção aperfeiçoada de álcool de crotila

Country Status (6)

Country Link
US (2) US10844404B2 (pt)
EP (1) EP3280808B1 (pt)
KR (1) KR20170134682A (pt)
CN (1) CN107750276A (pt)
BR (1) BR112017021667A2 (pt)
WO (1) WO2016164586A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111088209B (zh) * 2020-01-15 2021-07-30 南京工业大学 一种产1,4-丁二醇的重组丁醇梭菌及其构建方法与应用
CN116042450B (zh) * 2022-11-03 2024-10-01 杭州秀川科技有限公司 一株高效降解苯胺类物质的陶厄氏菌及其应用
CN115786211B (zh) * 2022-12-16 2024-10-15 杭州秀川科技有限公司 一种用于降解苯胺类物质的复合菌群及其应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2337027T3 (es) 1997-12-05 2010-04-20 Europaisches Laboratorium Fur Molekularbiologie (Embl) Nuevo metodo de clonacion de dna basado en el sistema de recombinacion rece-rect de e. coli.
US6355412B1 (en) 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
CN103080324B (zh) * 2010-05-05 2019-03-08 基因组股份公司 用于丁二烯生物合成的微生物和方法
BR112013001635A2 (pt) 2010-07-26 2016-05-24 Genomatica Inc micro-organismo e métodos para a biossíntese de aromáticos, 2, 4-pentadienoato e 1,3-butadieno
MY162964A (en) 2011-02-02 2017-07-31 Genomatica Inc Microorganisms and methods for the biosynthesis of butadiene
US9169486B2 (en) 2011-06-22 2015-10-27 Genomatica, Inc. Microorganisms for producing butadiene and methods related thereto
CN103890185A (zh) 2011-08-19 2014-06-25 基因组股份公司 用于生产2,4-戊二烯酸、丁二烯、丙烯、1,3-丁二醇和相关醇的微生物和方法
SG10201502101WA (en) 2011-09-16 2015-05-28 Genomatica Inc Microorganisms And Methods For Producing Alkenes
US9169496B2 (en) 2011-10-19 2015-10-27 Scientist of Fortune, S.A. Method for the enzymatic production of butadiene
WO2013090915A1 (en) * 2011-12-16 2013-06-20 Braskem S.A. Modified microorganisms and methods of making butadiene using same
WO2013130481A1 (en) * 2012-03-02 2013-09-06 Codexis, Inc. Recombinant host cells and processes for producing 1,3-butadiene through a crotonol intermediate
EP2861745A2 (en) 2012-06-15 2015-04-22 Invista Technologies S.à.r.l. Methods for biosynthesizing 1,3 butadiene
US8703455B2 (en) 2012-08-29 2014-04-22 Scientist of Fourtune, S.A. Production of volatile dienes by enzymatic dehydration of light alkenols
US20160040172A1 (en) 2013-03-15 2016-02-11 Genomatica, Inc. Microorganisms and methods for producing butadiene and related compounds by formate assimilation
WO2014184345A1 (en) 2013-05-17 2014-11-20 Global Bioenergies Alkenol dehydratase variants

Also Published As

Publication number Publication date
US20180135082A1 (en) 2018-05-17
EP3280808A1 (en) 2018-02-14
EP3280808B1 (en) 2020-09-16
WO2016164586A1 (en) 2016-10-13
US10844404B2 (en) 2020-11-24
CN107750276A (zh) 2018-03-02
KR20170134682A (ko) 2017-12-06
US20210277426A1 (en) 2021-09-09

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.