WO2018157457A1

WO2018157457A1 - Nano organic feed and preparation method therefor

Info

Publication number: WO2018157457A1
Application number: PCT/CN2017/080936
Authority: WO
Inventors: 曾济天
Original assignee: 曾济天
Priority date: 2017-03-03
Filing date: 2017-04-18
Publication date: 2018-09-07
Also published as: CN108522854A

Abstract

Provided are a nano organic feed and a preparation method therefor, relating to the field of environmental protection and feeds. The method for preparing a nano organic feed comprises: performing pulping, extraction, enzymolysis, disinfection, and pelletization on an organic waste. An organic feed is obtained according to the preparation method is provided. The method for preparing a nano organic feed is implemented by performing pulping, extraction, enzymolysis, disinfection, and pelletization on an organic waste, and has the characteristics of being accommodating to any raw material, low consumption and high efficiency, low costs, a simple process, and being suitable for industrial production. During the extraction process, the raw material is efficiently and thoroughly transformed for use without residues. The nano organic feed is obtained by the preparation method, has good economic benefits and wide feed use, and is a good complete organic feed.

Description

Nano organic feed and preparation method thereof

The present application claims priority to Chinese Patent Application No. CN201710126408.7, entitled "Nano-organic feed and its preparation method", filed on March 3, 2017, the entire contents of In this application.

Technical field

The invention relates to the field of environmental protection and feed, and in particular to a nano organic feed and a preparation method thereof.

Background technique

At present, the cost of feed in the aquaculture industry remains high. There are three major problems in the full-price feed formula of the corn-bean meal-fish meal type:

First, there is the problem of animal and human beings competing for food. Nowadays, the population is growing and the living standards are rising. Human beings are facing the problem of increasing food shortages.

The second is the issue of replacing corn oil with fossil fuels worldwide. The aquaculture industry with corn as the main feed source is bound to be greatly affected;

Third, there is a long-term shortage of feed protein resources, so it is imperative to find more cost-effective alternative sources of protein resources.

In addition, China's organic waste emissions are increasing at an annual rate of 8 to 10% per year, of which industrial organic wastewater (sugar liquid, yeast liquid, etc.) is produced in an amount of about 2 billion tons. Agricultural organic waste (animal waste, The amount of biogas slurry, crop straw, sawdust, vegetable and fruit residues, distiller's cake, mushroom residue, sputum, etc. is about 2.5 billion tons, and urban domestic garbage (food waste, urban sewage sludge) The production amount is about 190 million tons, less than 1/3 of the treated organic waste, and the harmless treatment rate is less than 10%. Even if the treated organic waste is treated, the negative impact on the environment is not eliminated. Therefore, organic waste is also a resource and is currently the only potential source of growth in the world.

Summary of the invention

The object of the present invention is to provide a preparation method of a nano organic feed. The preparation method uses the organic waste as a raw material to prepare a nano organic feed, which can solve the problems existing in the existing nano organic feed and can change the organic waste. Waste for treasure is reused with nano organic feed.

Another object of the present invention is to provide a nano organic feed which is prepared by the above preparation method and which is an excellent full-price nano organic feed.

The technical problem solved by the present invention is achieved by the following technical solutions.

The invention provides a preparation method of a nano organic feed, which comprises: refining, extracting, enzymatically hydrolyzing, sterilizing and granulating organic waste.

In a preferred embodiment of the invention, the organic waste is deodorized prior to refining the organic waste, and the deodorization is to add the first composite probiotic to the organic waste.

In a preferred embodiment of the invention, the first composite probiotic is selected from at least two of the group consisting of lactic acid bacteria, Bacillus, yeast, cellulase and glucanase.

In a preferred embodiment of the present invention, the organic waste is at least one selected from the group consisting of industrial organic waste, agricultural inorganic waste, and municipal solid waste, wherein the industrial organic waste is selected from the group consisting of molasses liquid and yeast liquid. At least one of the combination of the components; the agricultural organic waste is selected from the group consisting of livestock manure, biogas slurry, crop straw, sawdust, vegetable and fruit residue, dregs cake, mushroom residue and earthworm feces. At least one; the municipal solid waste is at least one selected from the group consisting of kitchen waste and urban sewage sludge.

In a preferred embodiment of the present invention, the organic waste is sorted and decontaminated prior to refining of the organic waste to obtain an organic waste free from impurities.

In a preferred embodiment of the invention, the organic waste free of impurities is pulverized and pulverized to particles of 3 to 5 mm.

In a preferred embodiment of the present invention, the refining is a process in which the organic waste is mixed with water in a mass ratio of 1:1 to 8 and the organic waste is ground to 5 to 10 μm.

In a preferred embodiment of the invention, at least one of supercritical extraction, microwave extraction, ultrasonic extraction, ultrahigh pressure extraction, and nanoabrasive extraction is used for the extraction.

In a preferred embodiment of the invention, the particles of the material in the extracted slurry are less than 1000 nm.

In a preferred embodiment of the present invention, the extraction is supercritical extraction, and the supercritical extraction is performed at a pressure of 25 to 80 MPa, a temperature of 0 to 40 ° C, and a flow rate of 1000 to 2000 L/h for 30 to 70 minutes; or 25 to 80 MPa pressure, 20 to 40 ° C temperature and 1000 to 2000 L / h flow rate extraction for 45 to 60 min; or at 30 to 76 MPa pressure, 20 to 30 ° C temperature and 1200 to 1700 L / h flow rate Extraction for 49 to 58 minutes; or extraction at a pressure of 41 to 49 MPa, a temperature of 22 to 27 ° C, and a flow rate of 1400 to 1500 L/h for 50 to 55 minutes.

In a preferred embodiment of the present invention, the extraction is microwave extraction, and the microwave extraction is performed at a pressure of 25 to 80 MPa, a temperature of 0 to 40 ° C, and a flow rate of 1000 to 3000 L/h for 30 to 70 minutes; or 25 to 30 minutes. Extraction at a pressure of 80 MPa, a temperature of 20 to 40 ° C and a flow rate of 1000 to 3000 L/h for 45 to 60 minutes; or a pressure of 30 to 76 MPa, a temperature of 20 to 30 ° C, and a flow rate of 1300 to 2500 L/h. ～60 min; or 50 to 55 min under a pressure of 41 to 49 MPa, a temperature of 22 to 27 ° C, and a flow rate of 2000 to 2300 L/h.

In a preferred embodiment of the invention, the extraction is ultrasonic extraction, and the ultrasonic extraction is at a frequency of 20 to 50 KHz. Extracting at a temperature of 0 to 40 ° C and a slurry volume of 1000 to 3000 L for 30 to 70 minutes; or extracting for 45 to 60 minutes at a frequency of 20 to 50 KHz, a temperature of 20 to 40 ° C, and a slurry volume of 1000 to 3000 L; It is extracted at a frequency of 20 to 40 KHz, a temperature of 20 to 30 ° C, and a slurry volume of 1800 to 2400 L for 45 to 60 minutes; or a frequency of 30 to 33 KHz, a temperature of 22 to 27 ° C, and a slurry of 2000 to 2300 L. Extract under volume for 50-55 min.

In a preferred embodiment of the present invention, the extraction is ultra-high pressure extraction, and the ultra-high pressure extraction is performed at a temperature of 250 to 380 MPa, 0 to 40 ° C and a flow rate of 1000 to 2000 L/h for 1 to 7 minutes; or at 250 380 MPa, 20 to 40 ° C temperature and 1000 ~ 2000 L / h flow rate over pressure 2 ~ 5min; or 250 ~ 350MPa, 20 ~ 30 ° C temperature and 1300 ~ 1600L / h flow rate over pressure 2 ~ 5 min; or overpressure for 2 to 5 min at a temperature of 300 to 380 MPa, 20 to 30 ° C and a flow rate of 1000 to 1500 L/h.

In a preferred embodiment of the present invention, the extraction is nano-abrasive extraction, and the nano-abrasive extraction is at a pressure of 50 to 80 MPa, a temperature of 0 to 40 ° C, and a slurry volume overpressure of 100 to 500 L for 1 to 7 min, or at 50. ～80MPa pressure, 0～40°C temperature and 1000～5000L/h flow rate overpressure for 1～7min; or pressure of 50～80MPa, temperature of 20～40°C and slurry volume overpressure of 100～500L 2 to 5 minutes, or a pressure of 50 to 80 MPa, a temperature of 20 to 40 ° C and a flow rate of 1000 to 5000 L / h for 2 to 5 minutes; or a pressure of 55 to 70 MPa, a temperature of 20 to 30 ° C and 200 to 400L of slurry volume overpressure for 2 to 5 minutes, or pressure of 55 to 70 MPa, temperature of 20 to 30 ° C and overpressure of 2000 to 4000 L / h for 2 to 5 min; or pressure of 60 to 65 MPa, 20 ~ The temperature of 30 ° C and the slurry volume of 300-350 L are overpressure for 2 to 5 min, or overpressure of 2 to 5 min at a pressure of 60 to 65 MPa, a temperature of 20 to 30 ° C, and a flow rate of 3000 to 3500 L/h.

In a preferred embodiment of the invention, the extraction is water or carbon dioxide as the extractant.

In a preferred embodiment of the present invention, the hydrolyzed slurry is inoculated with the second composite probiotic and cultured at a temperature of 30 to 50 ° C for 5 to 12 hours.

In a preferred embodiment of the present invention, the second composite probiotic is selected from at least two of the group consisting of lactic acid bacteria, Bacillus, yeast, cellulase, and glucanase, and the inoculum amount of the second compound probiotic It is 0.1% to 1% of the mass of the slurry.

In a preferred embodiment of the invention, the enzymatically obtained slurry is concentrated, dried, and then sterilized.

In a preferred embodiment of the invention, the concentration is reduced to 50 to 75 wt% of the hydrolyzed slurry to obtain a liquid nano-organic feed.

In a preferred embodiment of the invention, drying is to reduce the water content of the liquid organic feed to 10 to 15% by weight to obtain a solid nano-organic feed.

In a preferred embodiment of the invention, the sterilization is carried out using at least one of high pressure sterilization, microwave sterilization and ultrasonic sterilization.

A nano organic feed is prepared according to the above preparation method of nano organic feed.

The beneficial effects of the embodiments of the present invention are:

The preparation method of the nano organic feed according to the embodiment of the invention comprises the steps of refining, extracting, enzymatically disintegrating, sterilizing and granulating the organic waste, and has the advantages of no raw materials, low cost, high cost, simple process and suitable for industrial production. Features. In the extraction process, the raw materials are fully converted and utilized without any residue.

The nano organic feed of the embodiment of the invention is obtained by the above preparation method, has good economic benefits and wide use of feed, and is an excellent full-price nano organic feed.

detailed description

The technical solutions in the embodiments of the present invention will be clearly and completely described below in order to clarify the objects, the technical solutions and the advantages of the embodiments of the present invention. Those who do not specify the specific conditions in the examples are carried out according to the conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are conventional products that can be obtained by commercially available purchase.

The nano organic feed and the preparation method thereof according to the embodiments of the present invention are specifically described below.

A method for preparing a nano organic feed, comprising:

Organic waste is selected as a raw material for preparing nano organic feed. Organic waste includes industrial organic waste, agricultural inorganic waste, and municipal solid waste. Among them, industrial organic waste includes: molasses liquid and yeast liquid. Agricultural organic waste includes: livestock and poultry manure, biogas slurry, crop straw, sawdust wood chips, vegetable and fruit residues, dregs cakes, mushroom residues and earthworm droppings. Urban domestic garbage includes: kitchen waste, urban sewage sludge and so on. All of the above raw materials are organic raw materials and have a good raw material base.

Preferably, the above organic waste having no impurities is selected as a raw material for preparing the nano organic feed. It can also be understood that the above organic waste is selected and sorted to remove impurities contained therein. Sorting and removing impurities is to remove waste metal, waste plastics, waste glass, waste batteries and ceramic stones in organic waste to obtain organic waste without impurities.

In order to prevent the odor from being carried in the organic waste, the first composite probiotic is added to the organic waste, and the first compound probiotic is used to kill microorganisms and spoilage bacteria in the organic waste, thereby removing the odor. In this process, it is not necessary to limit the amount of the first composite probiotic, as long as the deodorizing effect and the cost are minimized.

The first composite probiotic is selected from at least two of the group consisting of lactic acid bacteria, Bacillus, yeast, cellulase, and glucanase. It is understood that the first composite probiotic is a mixed species of any ratio of two or more of the above plurality of active probiotics.

The organic waste after deodorization is refined to obtain a slurry. Specifically, after the organic waste and water are mixed according to a mass ratio of 1:1 to 8, in another example of the present invention, the organic waste and the water may be in a mass ratio of 1:2 to 6, or by mass ratio. After mixing 1:3 to 5, the mixed slurry is added to a colloid mill or a cone mill to The organic waste material can be ground to 5 to 10 μm particles.

In order to ensure the refining effect on organic waste, the organic waste without impurities is pulverized. For example, organic waste without impurities may be selected, or the organic waste may be sorted and mixed, and then added to a wet pulverizer to pulverize it to particles of 3 to 5 mm, followed by subsequent refining. This solution is not intended to limit the manner of pulverization, as long as the organic waste without impurities is pulverized to particles of 3 to 5 mm. Through the pulverization step, it is avoided that the raw material having a relatively large particle diameter is directly directly refined, resulting in uneven refining effect.

Through the refining step, the organic waste is prepared into a millimeter-scale cellulose slurry, thereby ensuring the preparation of the subsequent nano-scale nano-organic feed.

The refining slurry is subjected to extraction by at least one of supercritical extraction, microwave extraction, ultrasonic extraction, ultrahigh pressure extraction, and nanoabrasive extraction. It can be understood that the extraction can be carried out using one of the above various extraction methods, or can be carried out using at least two combinations of the above various extraction methods. As long as the particle size of the material in the extracted slurry is less than 1000 nm. Through the refining and extraction steps, the particle size of the prepared nano-organic feed can be ensured to be uniform, which is convenient for digestion and absorption of livestock.

When the extraction is supercritical extraction, it is extracted at a pressure of 25 to 80 MPa, a temperature of 0 to 40 ° C, and a flow rate of 1000 to 2000 L/h for 30 to 70 minutes. Preferably, the supercritical extraction is carried out at a pressure of 25 to 80 MPa, a temperature of 20 to 40 ° C, and a flow rate of 1000 to 2000 L/h for 45 to 60 minutes. In other examples of the present invention, the supercritical extraction is carried out at a pressure of 30 to 76 MPa, a temperature of 20 to 30 ° C, and a flow rate of 1200 to 1700 L/h for 49 to 58 minutes; or a pressure of 41 to 49 MPa, 22 to The temperature was extracted at a temperature of 27 ° C and a flow rate of 1400 to 1500 L / h for 50 to 55 minutes.

When the extraction is microwave extraction, the extraction is carried out at a pressure of 25-80 MPa, a temperature of 0 to 40 ° C, and a flow rate of 1000 to 3000 L/h for 30 to 70 minutes. Preferably, the microwave extraction is carried out at a pressure of 25 to 80 MPa, a temperature of 20 to 40 ° C, and a flow rate of 1000 to 3000 L/h for 45 to 60 minutes. In other examples of the present invention, the microwave extraction is performed at a pressure of 30 to 76 MPa, a temperature of 20 to 30 ° C, and a flow rate of 1300 to 2500 L/h for 45 to 60 minutes; or a pressure of 41 to 49 MPa, 22 to 27 The temperature of °C and the flow rate of 2000-2300 L/h were extracted for 50-55 min.

When the extraction is ultrasonic extraction, it is extracted at a frequency of 20 to 50 KHz, a temperature of 0 to 40 ° C, and a slurry volume of 1000 to 3000 L for 30 to 70 minutes. Preferably, the ultrasonic extraction is carried out at a frequency of 20 to 50 kHz, a temperature of 20 to 40 ° C, and a slurry volume of 1000 to 3000 L for 45 to 60 minutes. In other examples of the present invention, the ultrasonic extraction is performed at a frequency of 20 to 40 KHz, a temperature of 20 to 30 ° C, and a slurry volume of 1800 to 2400 L for 45 to 60 minutes; or a frequency of 30 to 33 KHz, 22 to 27 The temperature was °C and the slurry volume of 2000-2300 L was extracted for 50-55 min.

When the extraction is ultra-high pressure extraction, the pressure is over 1 to 7 minutes at a temperature of 250 to 380 MPa, 0 to 40 ° C and a flow rate of 1000 to 2000 L/h. Preferably, the ultrahigh pressure extraction is overpressure for 2 to 5 minutes at a temperature of 250 to 380 MPa, 20 to 40 ° C, and a flow rate of 1000 to 2000 L/h. In other examples of the invention, the ultrahigh pressure extraction is at a temperature of 250 to 350 MPa, 20 to 30 ° C. The degree is overpressure for 2 to 5 minutes at a flow rate of 1300 to 1600 L/h; or overpressure is 2 to 5 minutes at a temperature of 300 to 380 MPa, 20 to 30 ° C, and a flow rate of 1000 to 1500 L/h.

When the extraction is nano-abrasive extraction, it is at a pressure of 50 to 80 MPa, a temperature of 0 to 40 ° C, and a slurry volume overpressure of 100 to 500 L for 1 to 7 min, or a pressure of 50 to 80 MPa, a temperature of 0 to 40 ° C, and Overpressure for 1 to 7 minutes at 1000 to 5000 L/h flow rate. Preferably, the nano-milling extraction is at a pressure of 50 to 80 MPa, a temperature of 20 to 40 ° C, and a slurry volume overpressure of 100 to 500 L for 2 to 5 minutes, or a pressure of 50 to 80 MPa, a temperature of 20 to 40 ° C, and 1000. Overpressure for 2 to 5 minutes at ~5000L/h flow rate. In other examples of the present invention, the nano-abrasive extraction is at a pressure of 55 to 70 MPa, a temperature of 20 to 30 ° C, and a slurry volume overpressure of 200 to 400 L for 2 to 5 min, or a pressure of 55 to 70 MPa, 20 to 30. The temperature of °C and the overpressure of 2000~4000L/h flow rate are 2~5min; optionally, the nano-grinding extraction is at a pressure of 60-65MPa, a temperature of 20-30 °C and a slurry volume overpressure of 300-350L 2~ 5 min, or over 60 to 65 MPa, 20 to 30 ° C temperature and 3000 ~ 3500 L / h flow over pressure for 2 to 5 min.

When supercritical extraction and microwave extraction are carried out, the pressure is 25 to 80 MPa, the temperature of 0 to 40 ° C and the flow rate of 1000 to 2000 L/h are supercritically extracted for 5 to 10 minutes, and then the pressure is 25 to 80 MPa, and 0. Microwave extraction at a temperature of ～40 ° C and a flow rate of 1000 to 3000 L/h for 10 to 15 minutes.

When the extraction is supercritical extraction and ultrasonic extraction, it is supercritically extracted for 5 to 10 minutes at a pressure of 25 to 80 MPa, a temperature of 0 to 40 ° C, and a flow rate of 1000 to 2000 L/h, and then at a frequency of 20 to 50 KHz, 0. Ultrasonic extraction at a temperature of ～40 ° C and a slurry volume of 1000 to 3000 L for 10 to 15 minutes.

When the extraction is supercritical extraction and ultrahigh pressure extraction, it is supercritically extracted for 5 to 10 minutes at a pressure of 25 to 80 MPa, a temperature of 0 to 40 ° C, and a flow rate of 1000 to 2000 L/h, and then at 250 to 380 MPa, 0 to Overpressure for 2 to 5 minutes at a temperature of 40 ° C and a flow rate of 1000 to 2000 L/h.

When the extraction is supercritical extraction and nano-abrasive extraction, it is supercritically extracted for 5-10 minutes at a pressure of 25-80 MPa, a temperature of 0-40 ° C and a flow rate of 1000-2000 L/h, and then subjected to nano-abrasive extraction: at 50 ～80MPa pressure, 0～40°C temperature and 100～500L slurry volume overpressure for 10～15min, or over 50～80MPa pressure, 0～40°C temperature and 1000～5000L/h flow rate overpressure 10 ~15min.

When the extraction is microwave extraction and ultrasonic extraction, the microwave is extracted at a pressure of 25 to 80 MPa, a temperature of 0 to 40 ° C, a flow rate of 1000 to 3000 L/h for 10 to 15 minutes, and then at a frequency of 20 to 50 kHz, 0 to 40. Ultrasonic extraction was carried out for 10 to 15 minutes at a temperature of °C and a slurry volume of 1000 to 3000L.

When the extraction is microwave extraction and ultra-high pressure extraction, it is subjected to microwave extraction at a pressure of 25 to 80 MPa, a temperature of 0 to 40 ° C, a flow rate of 1000 to 3000 L/h for 10 to 15 minutes, and then at 250 to 380 MPa, 0 to Overpressure for 2 to 5 minutes at a temperature of 40 ° C and a flow rate of 1000 to 2000 L/h.

When the extraction is microwave extraction and nano-abrasive extraction, it is at a pressure of 25 to 80 MPa, a temperature of 0 to 40 ° C, Microwave extraction at a flow rate of 1000 to 3000 L/h for 10 to 15 minutes, followed by nano-abrasive extraction: at a pressure of 50 to 80 MPa, a temperature of 0 to 40 ° C, and a slurry volume overpressure of 100 to 500 L for 10 to 15 minutes, or The pressure is from 50 to 80 MPa, the temperature is from 0 to 40 ° C, and the overpressure is from 10 to 15 minutes at a flow rate of from 1000 to 5000 L/h.

When the extraction is ultrasonic extraction and ultra-high pressure extraction, ultrasonic extraction is performed at a frequency of 20 to 50 kHz, a temperature of 0 to 40 ° C, and a slurry volume of 1000 to 3000 L for 30 to 70 minutes, and then at 250 to 380 MPa, 0 to Overpressure for 2 to 5 minutes at a temperature of 40 ° C and a flow rate of 1000 to 2000 L/h.

When the extraction is ultrasonic extraction and nano-abrasive extraction, ultrasonic extraction is carried out at a frequency of 20 to 50 kHz, a temperature of 0 to 40 ° C, and a slurry volume of 1000 to 3000 L for 30 to 70 minutes, and then subjected to nano-abrasive extraction: at 50 to 80 MPa. Pressure, 0-40 ° C temperature and 100 ~ 500L slurry volume overpressure 10 ~ 15min, or pressure of 50 ~ 80MPa, 0 ~ 40 ° C temperature and 1000 ~ 5000L / h flow overpressure 10 ~ 15min .

The above is merely exemplified by the method of supercritical extraction, microwave extraction, ultrasonic extraction, ultra-high pressure extraction and nano-abrasive extraction, and a combination of two extraction methods for extraction. It should be understood that super Extraction by three or more extraction methods in critical extraction, microwave extraction, ultrasonic extraction, ultra-high pressure extraction and nano-abrasive extraction, and the order of any combination before and after extraction is not specifically limited, and all belong to the protection scope of the present invention. I will not list them here.

The above-mentioned combined extraction method has the characteristics of short processing time, high efficiency, low cost and high profit, and can ensure that the particle size of the material in the extracted slurry is less than 1000 nm, and the material having a particle diameter of 800-900 nm is mostly. Through extraction, the conversion of organic waste to nano-organic feed is improved, and the utilization rate of raw materials is high. Helps reduce costs and increase revenue.

According to the above, in the above extraction process, water or carbon dioxide is used as an extractant.

The extracted slurry is subjected to enzymatic hydrolysis. The second composite probiotic is inoculated into the hydrolyzed slurry at a temperature of 30 to 50 ° C, and maintained at the temperature for 5 to 12 hours to ensure sufficient enzymatic hydrolysis of the material in the slurry. By enzymatic hydrolysis, the particle size of the material in the slurry can be further reduced to ensure the preparation effect.

The inoculum of the second composite probiotic is preferably from 0.1% to 1% of the mass of the slurry. Preferably, the second composite probiotic is inoculated in an amount of from 0.3% to 0.7% by mass of the slurry. Further preferably, the second composite probiotic is inoculated in an amount of 0.5% by mass of the slurry.

The second composite probiotic is selected from at least two of the group consisting of lactic acid bacteria, Bacillus, yeast, cellulase, and glucanase. It is understood that the second composite probiotic is a mixed species of any ratio of two or more of the above plurality of active probiotics.

The enzymatically obtained slurry is concentrated to prepare a liquid nano-organic feed. When concentrated, the hydrolyzed slurry was added to a vacuum concentration apparatus and the water content was reduced to 50 to 75 wt%.

The liquid nano-organic feed is dried to prepare a solid nano-organic feed. When dry, the liquid will be The rice organic feed is added to the vacuum drying equipment and the water content is reduced to 10 to 15% by weight.

The solid nano organic feed is subjected to a sterilization step by one or at least two combinations of autoclaving, microwave sterilization and ultrasonic sterilization. Through the sterilization step, the microorganisms and the inactivation enzymes in the solid nano organic feed are killed to avoid the spoilage of the nano organic feed, and the preservation of the nano organic feed is also ensured.

The granulated nano-organic feed is granulated after sterilization. For example, the sterilized solid nano-organic feed is added to a pellet feeder to granulate it.

Preferably, before the granulation, a premix, a multivitamin, a composite probiotic, a protein and the like may be added to the solid nano organic feed, and the granulation becomes a full-price feed.

The prepared solid nano-organic feed is quantitatively packaged, that is, the entire preparation process of the nano-organic feed is completed.

The nano organic feed is prepared according to the above preparation method of the nano organic feed.

The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

Example 1

Organic wastes such as straw, wheat straw, corn stover, corn cob, bean straw and rapeseed cake are selected as raw materials for preparing nano organic feed. After removing waste materials such as scrap metal, waste plastics, waste glass, waste batteries, and ceramic stones from the above organic waste, spray lactic acid bacteria, Bacillus, yeast, cellulase, and dextran into organic waste. The mixed strain of the enzyme is deodorized. The deodorized organic waste was added to a wet pulverizer to pulverize the particles to 5 mm. The pulverized organic waste and water were mixed at a mass ratio of 1..8, and then added to a colloid mill, and the pulverized organic waste was ground to 8 μm pellets to obtain a slurry.

The fermented slurry was added to three series of ultrahigh pressure extraction equipments for three consecutive extractions, and the extraction conditions of each ultrahigh pressure extraction equipment were set at a pressure of 300 MPa, a temperature of 38 ° C, and a flow rate of 2000 L/h. Overpressure for 3 min.

To the extracted slurry, a mixed strain of lactic acid bacteria, Bacillus, yeast, cellulase, and glucanase having a slurry mass of 0.5% was inoculated, and cultured at 40 ° C for 8 hours.

The extracted slurry is concentrated under low temperature vacuum to have a water content of 50% to obtain a liquid nano organic feed. The liquid nano-organic feed is vacuum dried at a low temperature to a water content of 10% to obtain a solid nano-organic feed. The solid nano-organic feed was added to a static ultra-high pressure apparatus and sterilized at a pressure of 600 MPa and a temperature of 40 ° C for 5 minutes. Adding the solid nano-organic feed after sterilization to the pellet feed machine, no more than 60s and granulation at the instant of granulation The granulation is carried out under the condition that the temperature is not higher than 80 ° C, and the package is obtained.

The nano organic feed prepared in this example was used in the piglet test with the existing full-price feed. Thirty yorkshire × Rongchang black and half-bred piglets were selected as experimental group and fed with nano organic feed for 118 days. Thirty yorkshire × Rongchang black and half-bred piglets were selected as the control group and fed for 118 days with the existing full-price feed. By comparison, the daily gain of the piglets in the experimental group was increased by 10.2% compared with the daily weight gain of the control piglets; the feed weight of the piglets in the experimental group was reduced by 6% compared with the weight of the control piglets; the feed cost per kilogram of piglets in the experimental group was increased. Compared with the control group, the feed cost per kilogram of weight gain was reduced by 2.3 yuan, and the total cost of feed was reduced by 65% during the whole test period.

Example 2

Organic waste such as corn stover, corn cob, rapeseed cake, distiller's grains, cassava residue, bean dregs and dried chicken manure is selected as raw material for preparing nano organic feed. After removing waste materials such as scrap metal, waste plastics, waste glass, waste batteries, and ceramic stones from the above organic waste, spray lactic acid bacteria, Bacillus, yeast, cellulase, and dextran into organic waste. The mixed strain of the enzyme is deodorized. The deodorized organic waste was added to a wet pulverizer to pulverize the particles to 5 mm. The pulverized organic waste and water were mixed at a mass ratio of 1..6, and then added to a cone mill, and the pulverized organic waste was ground to particles of 10 μm to obtain a slurry.

The fermented slurry was added to a series extraction apparatus of an ultrasonic extraction apparatus and two ultrahigh pressure extraction apparatuses for three consecutive extractions. The extraction conditions of the ultrasonic extraction apparatus were set to a frequency of 40 KHz, a temperature of 40 ° C, and a slurry volume of 1000 L for 55 min. The extraction conditions for each ultrahigh pressure extraction apparatus were set to be overpressured for 5 min at a pressure of 360 MPa, a temperature of 40 ° C, and a flow rate of 1500 L/h.

To the extracted slurry, a mixed strain of lactic acid bacteria, Bacillus, yeast, cellulase, and glucanase having a slurry mass of 0.5% was inoculated, and cultured at 38 ° C for 9 hours.

The enzymatically obtained slurry is subjected to low-temperature vacuum concentration to have a water content of 45% to obtain a liquid nano-organic feed. The liquid nano-organic feed is vacuum dried at a low temperature to a water content of 9% to obtain a solid nano-organic feed. The solid nano-organic feed was added to a static ultra-high pressure apparatus and sterilized at a pressure of 600 MPa and a temperature of 40 ° C for 5 minutes. The sterilized solid nano-organic feed is added to the pellet feed machine, and granulated, packaged, and obtained at a granulation instant of not more than 60 s and a granulation temperature of not higher than 80 ° C.

The nano organic feed prepared in this example was used in the chicken test with the existing full-price feed. 500 5A-day 2A commercial chicks were selected as the experimental group, and they were fed with nano-organic feed for 52 days, and the whole period only gained 1.49 kg. A total of 500 5 day old 2A commercial chicks were selected as the control group, and the existing full-price feed was used for 52 days, and the whole period only gained 1.39 kg. By comparison, the average weight gain of chicks in the experimental group was 7.2% higher than that of the control chicks. The weight of chicks in the experimental group was 18.7% lower than that of the control chicks. The difference between the experimental group and the control group was extremely high. Significantly (P<0.01); the feed cost per kg of weight gain of the chicks in the test group was reduced by 1.15 yuan compared with the feed cost per kilogram of weight gain of the control chicks.

Example 3

Organic waste such as rice bran, blood meal, rapeseed cake, starch plant waste, bean dregs and dried chicken manure is selected as raw material for preparing nano organic feed. After removing waste materials such as scrap metal, waste plastics, waste glass, waste batteries, and ceramic stones from the above organic waste, spray lactic acid bacteria, Bacillus, yeast, cellulase, and dextran into organic waste. The mixed strain of the enzyme is deodorized. The deodorized organic waste was added to a wet pulverizer to pulverize the particles to 5 mm. The pulverized organic waste and water were mixed at a mass ratio of 1..5, and then added to a cone mill, and the pulverized organic waste was ground to 8 μm pellets to obtain a slurry.

The fermented slurry was added to a series extraction apparatus of an ultrasonic extraction apparatus and two ultrahigh pressure extraction apparatuses for three consecutive extractions. The extraction conditions of the ultrasonic extraction apparatus were set to a frequency of 40 KHz, a temperature of 30 ° C, and a slurry volume of 1200 L for 55 min. The extraction conditions for each ultrahigh pressure extraction apparatus were set to be overpressured for 5 min at a pressure of 270 MPa, a temperature of 30 ° C, and a flow rate of 1500 L/h.

To the hydrolyzed slurry, a mixed strain of lactic acid bacteria, Bacillus, yeast, cellulase, and glucanase having a slurry mass of 0.5% was inoculated, and cultured at 38 ° C for 7 hours.

The nano organic feed prepared in this example was used in the tilapia test with the existing full-price feed. Nine tilapia cultured in a closed hexahedral cage with a length of 5m × 4m × 2m was selected as the experimental group, and the whole culture cycle was fed with nano organic feed. Nine tilapia cultured in a closed hexahedral cage with a length of 5m × 4m × 2m was selected as the control group, and the whole breeding cycle was fed with the existing full-price feed. By comparison, the test group produced 141kg of fish per square meter, equivalent to 946m ² yield of 94097kg, and the control group produced 105.9kg per square meter of fish, equivalent to 666m ² yield of 70668.5kg. The yield of the test group increased by 33% compared with the control group. The feed coefficient of the test group averaged 1.93, and the feed coefficient of the control group averaged 2.53. The feed coefficient of the test group was reduced by 0.6 compared with the control group. The cost of feed per kilogram of tilapia in the test group was 1.78 yuan, and that of the control group was 3.16 yuan per kilogram of weight gain. The test group reduced the feed cost by 43.1% compared with the control group.

Example 4

Organic waste such as corn cob, rapeseed cake, bean dregs, dried chicken manure, fruit and vegetable residue and dried manure is selected as raw material for preparing nano organic feed. After removing waste materials such as scrap metal, waste plastics, waste glass, waste batteries, and ceramic stones from the above organic waste, spray lactic acid bacteria, Bacillus, yeast, cellulase, and dextran into organic waste. The mixed strain of the enzyme is deodorized. The deodorized organic waste was added to a wet pulverizer to pulverize the particles to 5 mm. Powder The crushed organic waste and water were mixed at a mass ratio of 1..7, and then added to a cone mill and the pulverized organic waste was ground to 10 μm pellets to obtain a slurry.

The fermented slurry was added to a supercritical extraction apparatus and a tandem extraction apparatus of two ultrahigh pressure extraction apparatuses for three consecutive extractions. The extraction conditions of the supercritical extraction apparatus were set to a pressure of 60 MPa, a temperature of 32 ° C, and a flow rate of 170 L/h for 6 min. The extraction conditions for each ultrahigh pressure extraction apparatus were set to be overpressured for 5 min at a pressure of 270 MPa, a temperature of 30 ° C, and a flow rate of 1500 L/h.

To the hydrolyzed slurry, a mixed strain of lactic acid bacteria, Bacillus, yeast, cellulase, and glucanase having a slurry mass of 0.5% was inoculated, and cultured at a temperature of 40 ° C for 10 hours.

The nano organic feed prepared in this example was used in the laying hen test with the existing full-price feed. 1200 Jingbai 3I commercial laying hens were selected as the experimental group and fed with nano organic feed for 300 days. 1200 Jingbai 3I commercial laying hens were selected as the control group and fed with the existing full-price feed for 300 days. By comparison, the test group had 126 eggs at 300 days of age, and 112 eggs at the age of 300 days in the control group. The egg production test group increased by 11% compared with the control group; the test group consumed 16.2 kg of feed per egg. In the control group, the cumulative consumption of feed for each laying hen was 15.8 kg; the ratio of the eggs to the test group was 2.93, and the ratio of the eggs to the control group was 2.52; the cost of the feed per kg of the test group was 0.55 yuan, and the cost of the feed per kg of the control group. The cost of feed for each kilogram of eggs in the test group was 1.23 yuan. The feed cost per kilogram of eggs produced by the test group was 2.04 yuan, and the test group was reduced by 0.81 yuan compared with the control group.

Example 5

Organic waste such as straw, wheat straw, bean straw, rapeseed cake and urban sewage sludge were selected as raw materials for preparing nano organic feed. The above-mentioned organic waste is sorted and decontaminated, and a mixed strain of lactic acid bacteria, cellulase, and glucanase is sprayed into the organic waste to deodorize. The deodorized organic waste was added to a wet pulverizer to pulverize the particles to 5 mm. The pulverized organic waste and water were mixed at a mass ratio of 1..6, and then added to a colloid mill, and the pulverized organic waste was ground to 8 μm pellets to obtain a slurry.

The fermented slurry was added to three series supercritical extraction equipments for three consecutive extractions, and the extraction conditions of each supercritical extraction device were set as follows: at a pressure of 76 MPa, a temperature of 30 ° C, and a flow rate of 1500 L/h. Extracted for 49 min.

To the extracted slurry, a mixed strain of lactic acid bacteria, Bacillus, and glucanase having a slurry mass of 0.5% was inoculated, and cultured at 40 ° C for 8 hours.

The extracted slurry is concentrated under low temperature vacuum to have a water content of 50% to obtain a liquid nano organic feed. The liquid nano-organic feed is vacuum dried at a low temperature to a water content of 10% to obtain a solid nano-organic feed. The solid nano-organic feed was added to a static ultra-high pressure apparatus and sterilized at a pressure of 600 MPa and a temperature of 40 ° C for 5 minutes. The sterilized solid nano-organic feed is added to the pellet feed machine, and granulated, packaged, and obtained at a granulation instant of not more than 60 s and a granulation temperature of not higher than 80 ° C.

Example 6

The method of Example 6 is substantially the same as that of Example 5, except that the fermented slurry is added to three series supercritical extraction devices for three consecutive extractions, and the extraction conditions of each supercritical extraction device are set. It is: extracting at a pressure of 449 MPa, a temperature of 27 ° C and a flow rate of 1400 L / h for 50 min, and finally obtaining a nano organic feed.

Example 7

Organic waste such as corn stover, corn cob, rapeseed cake, distiller's grains, cassava residue, bean dregs and dried chicken manure is selected as raw material for preparing nano organic feed. The organic waste described above is sorted and decontaminated, and a mixed strain of yeast, cellulase, and glucanase is sprayed into the organic waste to deodorize. The deodorized organic waste was added to a wet pulverizer to pulverize the particles to 5 mm. The pulverized organic waste and water were mixed at a mass ratio of 1..5, and then added to a cone mill, and the pulverized organic waste was ground to 10 μm pellets to obtain a slurry.

The fermented slurry was added to three microwave extraction apparatuses for three consecutive extractions. The extraction conditions of the microwave extraction apparatus were set as follows: extraction at a pressure of 76 MPa, a temperature of 30 ° C, and a flow rate of 2000 L/h for 45 min.

To the extracted slurry, a mixed strain of yeast, cellulase, and glucanase having a slurry mass of 0.5% was inoculated, and cultured at 38 ° C for 9 hours.

Example 8

The method of Example 8 is substantially the same as that of Example 7, except that the fermented slurry is added to three series supercritical extraction devices for three consecutive extractions, and the extraction conditions of each supercritical extraction device are set. It is: extracting at a pressure of 49 MPa, a temperature of 27 ° C and a flow rate of 2200 L / h for 50 min, and finally obtaining a nano organic feed.

Example 9

Organic waste such as rice bran, blood meal, rapeseed cake, starch plant waste, bean dregs and dried chicken manure is selected as raw material for preparing nano organic feed. Waste metal, waste plastic, waste glass, waste battery and ceramic stone in the above organic waste After the impurities are removed, a mixed strain of lactic acid bacteria, cellulase, and glucanase is sprayed into the organic waste to deodorize. The deodorized organic waste was added to a wet pulverizer to pulverize the particles to 5 mm. The pulverized organic waste and water were mixed at a mass ratio of 1..3, and then added to a cone mill, and the pulverized organic waste was ground to 8 μm pellets to obtain a slurry.

The fermented slurry was added to three ultrasonic extraction apparatuses for three consecutive extractions. The extraction conditions of the ultrasonic extraction apparatus were set to be 45 min extraction at a frequency of 40 KHz, a temperature of 30 ° C, and a slurry volume of 2000 L.

To the hydrolyzed slurry, a mixed strain of lactic acid bacteria, cellulase, and glucanase having a slurry mass of 0.5% was inoculated, and cultured at a temperature of 38 ° C for 7 hours.

Example 10

The method of Example 10 is substantially the same as that of Example 9, except that the fermented slurry is added to three serial ultrasonic extraction apparatuses for three consecutive extractions, and the extraction conditions of each ultrasonic extraction apparatus are set as follows: The extraction was carried out for 50 min at a frequency of 33 KHz, a temperature of 27 ° C and a slurry volume of 2100 L, and finally a nano organic feed was obtained.

Example 11

Organic waste such as corn cob, rapeseed cake, bean dregs, dried chicken manure, fruit and vegetable residue and dried manure is selected as raw material for preparing nano organic feed. After removing the waste metal such as scrap metal, waste plastic, waste glass, waste battery, and ceramic stone in the above organic waste, spray the mixed bacteria of lactic acid bacteria, Bacillus, and glucanase into the organic waste to remove smelly. The deodorized organic waste was added to a wet pulverizer to pulverize the particles to 5 mm. The pulverized organic waste and water were mixed at a mass ratio of 1.. 2, and then added to a cone mill, and the pulverized organic waste was ground to particles of 10 μm to obtain a slurry.

The fermented slurry was added to three ultrahigh pressure extraction equipments for three consecutive extractions. The extraction conditions for the ultrahigh pressure extraction equipment were set to be overpressured for 2 min at a temperature of 350 MPa, 30 ° C and a flow rate of 1500 L/h.

To the hydrolyzed slurry, a mixed strain of lactic acid bacteria, Bacillus, and glucanase having a slurry mass of 0.5% was inoculated, and cultured at a temperature of 40 ° C for 10 hours.

The enzymatically obtained slurry is subjected to low-temperature vacuum concentration to have a water content of 45% to obtain a liquid nano-organic feed. The liquid nano-organic feed is vacuum dried at a low temperature to a water content of 9% to obtain a solid nano-organic feed. Add solid nano-organic feed to static ultra-high pressure equipment, sterilize at 600MPa and 40°C 5min. The sterilized solid nano-organic feed is added to the pellet feed machine, and granulated, packaged, and obtained at a granulation instant of not more than 60 s and a granulation temperature of not higher than 80 ° C.

Example 12

The method of Example 12 is substantially the same as that of Example 11, except that the fermented slurry is added to three ultrahigh pressure extraction apparatuses connected in series for three consecutive extractions, and the extraction of each ultrahigh pressure extraction apparatus is set. The conditions were: overpressure at a temperature of 380 MPa, 30 ° C and a flow rate of 1200 L/h for 2 min.

Example 13

The method of Example 13 is substantially the same as that of Example 11, except that the fermented slurry is added to three tandem nano-grinding extraction apparatuses for three consecutive extractions, and the extraction of each nano-abrasive extraction apparatus is set. The conditions were: a pressure of 70 MPa, a temperature of 30 ° C, and a slurry volume of 200 L for 2 min.

Example 14

The method of Example 14 is substantially the same as that of Example 11, except that the fermented slurry is added to three tandem nano-grinding extraction apparatuses for three consecutive extractions, and the extraction of each nano-abrasive extraction apparatus is set. The conditions were: overpressure at a pressure of 65 MPa, a temperature of 30 ° C and a flow rate of 3200 L/h for 2 min.

The nano organic feeds prepared in Examples 5 to 14 and the existing full-price feeds were separately used for the laying hen test. 1200 Jingbai 3I commercial laying hens were selected as the experimental group, and the nano organic feeds prepared in Examples 5 to 14 were used for feeding for 300 days. 1200 Jingbai 3I commercial laying hens were selected as the control group and fed with the existing full-price feed for 300 days. By comparison, the egg production in the experimental group was increased by 10-15% compared with the control group at 300 days of age; the feed cost per kilogram of eggs in the experimental group was reduced by 0.6-0.9 yuan compared with the control group.

It can be seen from Examples 1 to 14 that the nano-organic feed is a safe and reliable feed, and has achieved considerable yield increase or synergy effects in pigs, chickens and fish.

In summary, the preparation method of the nano organic feed according to the embodiment of the present invention prepares the organic waste by refining, extracting, enzymatically disintegrating, sterilizing and granulating, and has the advantages of no raw materials, low cost, high cost, low cost and simple process. And suitable for industrial production and other characteristics. In the extraction process, the raw materials are fully converted and utilized without any residue.

The nano organic feed of the embodiment of the invention is prepared by the above preparation method, has good economic benefits and wide use of feed and animal, and is an excellent full-price nano organic feed.

The embodiments described above are a part of the embodiments of the invention, and not all of the embodiments. The detailed description of the embodiments of the invention is not intended to All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative efforts are within the scope of the present invention.

Claims

A method for preparing a nano organic feed, characterized in that it comprises:

The organic waste is refined, extracted, hydrolyzed, sterilized and granulated.
The method for preparing a nano organic feed according to claim 1, further comprising deodorizing the organic waste before the organic waste is refining, and deodorizing is to add to the organic waste. The first compound probiotic.
The method for preparing a nano organic feed according to claim 2, wherein the first composite probiotic is at least two selected from the group consisting of a lactic acid bacterium, a bacillus, a yeast, a cellulase, and a glucanase. Kind.
The method for producing a nano organic feed according to any one of claims 1 to 3, wherein the organic waste is at least selected from the group consisting of industrial organic waste, agricultural inorganic waste, and municipal solid waste. In one aspect, the industrial organic waste is selected from at least one of a combination consisting of a molasses liquid and a yeast liquid; the agricultural organic waste is selected from the group consisting of livestock manure, biogas slurry, crop straw, sawdust wood chips At least one of a combination of a vegetable and fruit residue, a dregs cake, a mushroom residue, and a manure; the municipal solid waste is selected from at least one of a combination of a kitchen waste and a municipal sludge.
The method for preparing a nano organic feed according to any one of claims 1 to 4, characterized in that before the organic waste is refining, the organic waste is sorted and decontaminated to obtain an impurity-free Organic waste.
The method for producing a nano organic feed according to claim 5, wherein the organic waste free from impurities is pulverized and pulverized to particles of 3 to 5 mm.
The method for preparing a nano organic feed according to any one of claims 1 to 6, wherein the refining is performed by mixing the organic waste with water in a mass ratio of 1:1 to 8 The organic waste is ground to particles of 5 to 10 μm.
The method for preparing a nano organic feed according to any one of claims 1 to 7, wherein the extraction comprises at least one of supercritical extraction, microwave extraction, ultrasonic extraction, ultrahigh pressure extraction, and nano abrasive extraction. .
The method for preparing a nano organic feed according to any one of claims 1 to 7, wherein the particles of the material in the extracted slurry are less than 1000 nm.
The method for preparing a nano organic feed according to any one of claims 1 to 9, wherein the extraction is supercritical extraction, and the supercritical extraction is at a pressure of 25 to 80 MPa, 0 to 40 °C. Warm And extraction at a flow rate of 1000 to 2000 L/h for 30 to 70 minutes; or extraction at a pressure of 25 to 80 MPa, a temperature of 20 to 40 ° C, and a flow rate of 1000 to 2000 L/h for 45 to 60 minutes; or 30 to 76 MPa. The pressure, the temperature of 20 ~ 30 ° C and the flow rate of 1200 ~ 1700L / h extraction 49 ~ 58min; or the pressure of 41 ~ 49MPa, 22 ~ 27 ° C temperature and 1400 ~ 1500L / h flow rate extraction 50 ~ 55min.
The method for preparing a nano organic feed according to any one of claims 1 to 9, wherein the extraction is microwave extraction, and the microwave extraction is at a pressure of 25 to 80 MPa, a temperature of 0 to 40 ° C, Extraction at a flow rate of 1000 to 3000 L/h for 30 to 70 minutes; or extraction at a pressure of 25 to 80 MPa, a temperature of 20 to 40 ° C, and a flow rate of 1000 to 3000 L/h for 45 to 60 minutes; or a pressure of 30 to 76 MPa. , extraction at a temperature of 20 to 30 ° C and a flow rate of 1300 to 2500 L / h for 45 to 60 min; or extraction of 50 to 55 min at a pressure of 41 to 49 MPa, a temperature of 22 to 27 ° C, and a flow rate of 2000 to 2300 L / h.
The method for preparing a nano organic feed according to any one of claims 1 to 9, wherein the extraction is ultrasonic extraction, and the ultrasonic extraction is at a frequency of 20 to 50 kHz, a temperature of 0 to 40 ° C, and Extracting from 1000 to 3000 L of slurry volume for 30 to 70 min; or extracting at a frequency of 20 to 50 KHz, a temperature of 20 to 40 ° C and a slurry volume of 1000 to 3000 L for 45 to 60 min; or at a frequency of 20 to 40 KHz. , extraction at a temperature of 20 to 30 ° C and a slurry volume of 1800 to 2400 L for 45 to 60 minutes; or extraction at a frequency of 30 to 33 KHz, a temperature of 22 to 27 ° C, and a slurry volume of 2000 to 2300 L for 50 to 55 minutes.
The method for preparing a nano organic feed according to any one of claims 1 to 9, wherein the extraction is ultrahigh pressure extraction, and the ultrahigh pressure extraction is at a temperature of 250 to 380 MPa, 0 to 40 ° C and Overpressure for 1 to 7 minutes at a flow rate of 1000 to 2000 L/h; or overpressure for 2 to 5 minutes at a temperature of 250 to 380 MPa, 20 to 40 ° C and a flow rate of 1000 to 2000 L/h; or 250 to 350 MPa, 20 Overpressure for ～30°C and flow rate of 1300～1600L/h for 2～5min; or overpressure for 2～5min at temperatures of 300～380MPa, 20～30°C and flow rate of 1000～1500L/h.
The method for preparing a nano organic feed according to any one of claims 1 to 9, wherein the extraction is nano-grinding extraction, and the nano-grinding extraction is at a pressure of 50 to 80 MPa, 0 to 40 ° C. The temperature and the slurry volume of 100-500L are overpressured for 1 to 7 minutes, or at a pressure of 50 to 80 MPa, a temperature of 0 to 40 ° C, and an overpressure of 1 to 7 min at a flow rate of 1000 to 5000 L/h; or 50 to 80 MPa. Pressure, temperature of 20 to 40 ° C and slurry volume of 100 to 500 L overpressure for 2 to 5 min, or pressure of 50 to 80 MPa, temperature of 20 to 40 ° C and overpressure of 1000 to 5000 L / h for 2 to 5 min; Or at a pressure of 55 to 70 MPa, a temperature of 20 to 30 ° C, and a slurry volume overpressure of 200 to 400 L for 2 to 5 minutes, or a pressure of 55 to 70 MPa, a temperature of 20 to 30 ° C, and a flow rate of 2000 to 4000 L/h. Under pressure for 2 to 5 minutes; or at a pressure of 60 to 65 MPa, a temperature of 20 to 30 ° C and The slurry volume of 300-350 L is overpressured for 2 to 5 minutes, or overpressured for 2 to 5 minutes at a pressure of 60 to 65 MPa, a temperature of 20 to 30 ° C, and a flow rate of 3000 to 3500 L/h.
The method for producing a nano organic feed according to any one of claims 1 to 14, wherein the extraction is water or carbon dioxide as an extractant.
The method for preparing a nano organic feed according to claim 1, wherein the enzymatic hydrolysis is to inoculate the hydrolyzed slurry with a second composite probiotic and culture at a temperature of 30 to 50 ° C for 5 to 5 12h.
The method for preparing a nano organic feed according to claim 16, wherein the second composite probiotic is at least two selected from the group consisting of lactic acid bacteria, bacillus, yeast, cellulase and glucanase. The inoculum of the second composite probiotic is 0.1% to 1% of the mass of the slurry.
The method for preparing a nano organic feed according to claim 1, wherein the slurry after enzymatic hydrolysis is concentrated, dried, and then sterilized.
The method for preparing a nano organic feed according to claim 18, wherein the concentration is to reduce the water content in the slurry after enzymatic hydrolysis to 50 to 75 wt%, to obtain the liquid nano-organic feed. .
The method for preparing a nano organic feed according to claim 19, wherein the drying is to reduce the water content in the liquid organic feed to 10 to 15% by weight to obtain the solid nano-organic feed.
The method for preparing a nano organic feed according to claim 1, wherein the sterilization is performed by at least one of high pressure sterilization, microwave sterilization, and ultrasonic sterilization.
A nano organic feed obtained by the method for producing a nano organic feed according to any one of claims 1 to 21.