CN105175129A - Nano microbial agent and preparation method thereof - Google Patents
Nano microbial agent and preparation method thereof Download PDFInfo
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- CN105175129A CN105175129A CN201510568952.8A CN201510568952A CN105175129A CN 105175129 A CN105175129 A CN 105175129A CN 201510568952 A CN201510568952 A CN 201510568952A CN 105175129 A CN105175129 A CN 105175129A
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Abstract
The invention provides a nano microbial agent. Raw materials comprise maltose nanoparticles, nano powdered rock phosphate, nano LaCoO3, kitchen waste, mushroom residue, rice bran, chicken manure, straw powder, waste molasses, blumea oil, rhodiola rosea extracts, active rare earth, boric acid, biological humic acid, microelement fertilizers, brassinolide, indolebutyric acid potassium and a complex microbial agent. The invention also provides a preparation method of the microbial agent. According to the nano microbial agent, the number of living bacteria is 0.68 to 0.72 billion/g, the total nitrogen content is 8 percent by weight, the total phosphorus content is 4 percent by weight, the total potassium content is 12 percent by weight, the content of organic matters based on dry basis is 45 percent by weight, the water content is 10 percent by weight, the pH value is 6.2 to 6.5, and the contaminating microorganism rate is 3.4 percent. After being applied, the nano microbial agent has the effects of increasing production and improving crop quality and has an obvious soil improvement effect.
Description
Technical field
The present invention relates to a kind of nano microbeam microbial inoculum and preparation method thereof, belong to microbial-bacterial fertilizer technical field.
Background technology
Along with a large amount of uses of chemical fertilizer, it has been well known fact that its utilization ratio constantly reduces, long-term application chemical fertilizer, causes soil compaction, disease and pest increases the weight of, and existing chemical fertilizer is generally acidity and lacks phosphorus decomposing function, due to water insoluble with the phosphorus of the shape such as calcium monohydrogenphosphate, calcium phosphate existence in soil, can not be absorbed by plants, cause the disappearance of phosphorus to waste, and cause soil compaction, water holding capacity declines, and the soil weight improves.This illustrates, it is limited for only improving crop yield by a large amount of application fertilizer, also has a series of problems such as contaminate environment still more.
Various countries scientist is making great efforts to explore always and is improving chemical fertilizer utilization ratio and reach balance fertilizing, the rational application of fertilizer to overcome the approach of its drawback for this reason, and microbial fertilizer has original effect in solution this respect problem.
But existing microbial fertilizer, still has the following disadvantages:
(1) living bacteria count amount is low, and N-P-K content is low, organic content is low, and miscellaneous bacteria is many, and heavy metal content exceeds standard.
(2) limited to the yield and quality improvement result of crop.
(3) not good to the improved effect of soil.
Summary of the invention
The present invention, for solving above technical problem, provides a kind of nano microbeam microbial inoculum and preparation method thereof, to realize following goal of the invention:
(1) the nano microbeam microbial inoculum prepared of the present invention, living bacteria count amount is 6.8-7.2 hundred million/g, total nitrogen content is 8wt%, total phosphorous is 4wt%, total potassium content is 12wt%, and organic (in butt) is 45wt%, water ratio 10wt%, pH is 6.2-6.5, granule strength is 9.2N, and granularity (2-3mm) is 98.6%, and miscellaneous bacteria rate is 3.4%, in butt, total arsenic content≤1.8mg/kg, total cadmium content≤0.4mg/kg, total lead content≤7.5mg/kg, total chrome content≤15mg/kg, total mercury content≤0.1mg/kg.
(2) the nano microbeam microbial inoculum prepared of the present invention, after using, has volume increase and improves the effect of crop quality.
(3) the nano microbeam microbial inoculum prepared of the present invention, has the effect of significantly improvement soil.
For solving above technical problem, the technical solution used in the present invention is as follows:
A kind of nano microbeam microbial inoculum, each raw material comprises maltose Nano microsphere, nanometer ground phosphate rock, nanometer LaCoO
3, rubbish from cooking, mushroom residue, rice bran, chicken manure, straw powder, waste molasses, Oleum Folium Artemisiae Argyi, Radix Rhodiolae extract, active rare earth, boric acid, biological humic, trace element fertilizer, brassinolide, butyric acid potassium, complex microbial inoculum.
Below the further improvement to technique scheme:
The mass ratio of each raw material is: maltose Nano microsphere 3 parts, 5 parts, nanometer ground phosphate rock, nanometer LaCoO
34 parts, rubbish from cooking 20 parts, mushroom residue 12 parts, 11 parts, rice bran, chicken manure 20 parts, straw powder 13 parts, waste molasses 5 parts, Oleum Folium Artemisiae Argyi 2 parts, Radix Rhodiolae extract 4 parts, active rare earth 3 parts, boric acid 3 parts, biological humic 10 parts, trace element fertilizer 2 parts, brassinolide 0.1 part, butyric acid potassium 2 parts, complex microbial inoculum 0.3 part.
Described maltose Nano microsphere, volume average particle size is 0.55-0.56 μm, and aerodynamic diameter is 5.334-5.456 μm;
Described chicken manure, organic content is 45wt%; Described straw powder, diameter is 2-3mm; Described waste molasses, full sugar is 50wt%, brix 80 degree.
Described Oleum Folium Artemisiae Argyi, the refractive index of 25 DEG C is 1.4650-1.4770, and the relative density of 25 DEG C is 0.89-0.91, and the content of tarragon alcohol is 99.9wt%; Described Radix Rhodiolae extract, powder, moisture <12%, total percent of ash <8%, acid-insoluble ash <2%, total Luo Sai dimension hplc is 5%, and salidroside content is 1%.
Described active rare earth, khaki color powder, REO is 40%, moisture content <0.5%, and granularity is 100 orders, and lanthanum content is 12%, and cerium content is 10.33%, and praseodymium content is 5.66%, and neodymium content is 3.5%; Described trace element: boric acid, zinc oxide, ferrous sulfate, manganous carbonate, molybdic oxide, mass ratio is 2:3:2:1.
Described complex microbial inoculum, for liquid bacteria suspension, the concentration of its moderate light rhodospirillum is 0.2 hundred million cfu/ml, the concentration of Brasil diazotrophic spirillum is the concentration of 0.3 hundred million cfu/ml, Bai Yelin kirschner bacterium is 0.1 hundred million cfu/ml, the concentration of wood sugar glyconic acid acetobacter is 0.1 hundred million cfu/ml, the concentration of subtilis is 0.3 hundred million cfu/ml, and the concentration of plant lactobacillus is 0.4 hundred million cfu/ml.
Described nano microbeam microbial inoculum, living bacteria count amount is 6.8-7.2 hundred million/g, and total nitrogen content is 8wt%, and total phosphorous is 4wt%, and total potassium content is 12wt%, and in butt, organic content is 45wt%.
A preparation method for nano microbeam microbial inoculum, comprises and prepares maltose Nano microsphere, preparation nanometer acidifying ground phosphate rock, preparation nanometer LaCoO
3, rubbish from cooking process, spray composite fungus agent.
Describedly prepare maltose Nano microsphere, get the maltose of 2 weight parts, the polymerization degree of 8 weight parts is the chitosan of 50CPS, be dissolved in the acetic acid solution of 500ml0.6%, mixing, by mixed solution through 0.45 μm of micro-pore-film filtration, obtain filtrate, carry out spraying dry, inlet temperature is 110 DEG C, and input speed is 4ml/min, obtain maltose Nano microsphere, state is powder.
Described preparation nanometer LaCoO
3, by LaCl
3, CoCl
2, NaOH mixes according to the mass ratio of 1:1:6:10, stir into pasty state with the deionized water of 10%, under 500r/min, ball milling 2h obtains precursor matter, by precursor matter at 800 DEG C after roasting 2h, with deionized water wash, after chlorion is cleaned completely, dry 5h for 120 DEG C, obtained nanometer LaCoO
3.
Adopt above technical scheme, beneficial effect of the present invention is:
(1) the nano microbeam microbial inoculum prepared of the present invention, living bacteria count amount is 6.8-7.2 hundred million/g, total nitrogen content is 8wt%, total phosphorous is 4wt%, total potassium content is 12wt%, and organic (in butt) is 45wt%, water ratio 10wt%, pH is 6.2-6.5, granule strength is 9.2N, and granularity (2-3mm) is 98.6%, and miscellaneous bacteria rate is 3.4%, in butt, total arsenic content≤1.8mg/kg, total cadmium content≤0.4mg/kg, total lead content≤7.5mg/kg, total chrome content≤15mg/kg, total mercury content≤0.1mg/kg.
(2) the nano microbeam microbial inoculum prepared of the present invention, for the plantation of cucumber, output reaches 6520kg/ mu, volume increase 19%, and Vitamin C content is 0.073mg/100g, and increase by 8.6%, total sugar content is 1.43%, increases by 8.9%.
(3) the nano microbeam microbial inoculum prepared of the present invention, for great Jiang plantation, output reaches 1.4 ten thousand pounds/acre, volume increase 25%, and for planting garlic, output reaches 1600kg/ mu, volume increase 18%; For potato plantation, output reaches 2800kg/ mu, volume increase 24%.
(4) the nano microbeam microbial inoculum prepared of the present invention, also has a better role to growing area soil,
After planting one batch of crop, soil organic matter content is 1.7%, and alkali-hydrolyzable nitrogen is 150mg/kg, and available potassium is 160mg/kg, and available phosphorus is 110mg/kg, and soil pH is 6.2, and the soil weight is 1.1g/cm
3, soil porosity is 58%, and Soil Microorganism content is 250 × 10
5individual/g, soil moisture content is 28%, and in soil, organic carbon content is 33g/kg; By the power of 100N, the waddy that diameter is 1cm is inserted soil, the degree of depth that waddy enters soil is 15cm, comparatively uses Common compound fertilizer, and the degree of depth that waddy enters soil increases by 60%.
Before plantation, soil indices is: soil organic matter content is 1.1%, and alkali-hydrolyzable nitrogen is 110mg/kg, and available potassium is 120mg/kg, and available phosphorus is 68mg/kg, and soil pH is 5.6, and the soil weight is 1.5g/cm
3, soil porosity is 41%.
Embodiment
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein is only for instruction and explanation of the present invention, is not intended to limit the present invention.
embodiment 1 one kinds of nano microbeam microbial inoculums
described microbiobacterial agent, the mass ratio of each feed composition is:
Maltose Nano microsphere 3 parts, 5 parts, nanometer ground phosphate rock, nanometer LaCoO
34 parts, rubbish from cooking 20 parts, mushroom residue 12 parts, 11 parts, rice bran, chicken manure 20 parts, straw powder 13 parts, waste molasses 5 parts, Oleum Folium Artemisiae Argyi 2 parts, Radix Rhodiolae extract 4 parts, active rare earth 3 parts, boric acid 3 parts, biological humic 10 parts, trace element fertilizer 2 parts, brassinolide 0.1 part, butyric acid potassium 2 parts, complex microbial inoculum 0.3 part.
Described maltose Nano microsphere, volume average particle size is 0.55-0.56 μm, and aerodynamic diameter is 5.334-5.456 μm.
Described chicken manure, organic content is 45wt%;
Described straw powder, diameter is 2-3mm;
Described waste molasses, full sugar is 50wt%, brix 80 degree;
Described Oleum Folium Artemisiae Argyi, the refractive index of 25 DEG C is 1.4650-1.4770, and the relative density of 25 DEG C is 0.89-0.91, and the content of tarragon alcohol is 99.9wt%;
Described Radix Rhodiolae extract, powder, moisture <12%, total percent of ash <8%, acid-insoluble ash <2%, total Luo Sai dimension hplc is 5%, and salidroside content is 1%;
Described active rare earth, khaki color powder, REO is 40%, moisture content <0.5%, and granularity is 100 orders, and lanthanum content is 12%, and cerium content is 10.33%, and praseodymium content is 5.66%, and neodymium content is 3.5%;
Described trace element: boric acid, zinc oxide, ferrous sulfate, manganous carbonate, molybdic oxide, mass ratio is 2:3:2:1;
Described complex microbial inoculum is liquid bacteria suspension, degree of comprising light rhodospirillum, Brasil diazotrophic spirillum, Bai Yelin kirschner bacterium, wood sugar glyconic acid acetobacter, subtilis, plant lactobacillus; The concentration of its moderate light rhodospirillum is 0.2 hundred million cfu/ml, the concentration of Brasil diazotrophic spirillum is the concentration of 0.3 hundred million cfu/ml, Bai Yelin kirschner bacterium is 0.1 hundred million cfu/ml, the concentration of wood sugar glyconic acid acetobacter is 0.1 hundred million cfu/ml, the concentration of subtilis is 0.3 hundred million cfu/ml, and the concentration of plant lactobacillus is 0.4 hundred million cfu/ml.
Described degree light rhodospirillum (
rhodospirillumphotometricum), culture presevation number is ACCC00072; Brasil diazotrophic spirillum (
azospirillumbrasilense), culture presevation number is: ACCC10102
Bai Yelin kirschner bacterium (
beijerinckiasp.), culture presevation number is ACCC01435; Wood sugar glyconic acid acetobacter (
gluconacetobacterxylinus), culture presevation number is ACCC10215; Subtilis (
bacillussubtilis), culture presevation number is CGMCC1.1630; Plant lactobacillus (
lactobacillusplantarum), culture presevation number is ACCC10171.
Above-mentioned bacterial classification source is commercially available, purchases available.
the preparation method of embodiment 2 one kinds of nano microbeam microbial inoculums
(1) raw material is taken
According to the mass ratio of each raw material of nano microbeam microbial inoculum, take each raw material.
(2) maltose Nano microsphere is prepared
The preparation of described maltose Nano microsphere, get the maltose of 2 weight parts, the polymerization degree of 8 weight parts is the chitosan of 50CPS, be dissolved in the acetic acid solution of 500ml0.6%, mixing, by mixed solution through 0.45 μm of micro-pore-film filtration, obtain filtrate, carry out spraying dry, obtain maltose Nano microsphere, state is powder; Described spraying dry, inlet temperature is 110 DEG C, and input speed is 4ml/min.
(3) nanometer acidifying ground phosphate rock is prepared
The preparation of described nanometer acidifying ground phosphate rock, ground phosphate rock is crushed to 200 orders, get ground phosphate rock 1000kg, add 1070kg aqueous sulfuric acid and (get industrial sulphuric acid (93%) 300kg, add in 770kg water, be mixed with phosphate aqueous solution), react 0.5 hour, make acidic phosphorus mine powder slurry, described acidic phosphorus mine powder slurry, place after 5 hours, 15000r/min speed down cut 13 minutes, add the weathered coal (content of humic acid is 45%) in the mistake 100 mesh sieve hole of 25%, 12000r/min down cut 10 minutes, dry, be crushed to 1-50nm, obtain nanometer acidifying ground phosphate rock.
(4) nanometer LaCoO is prepared
3
Described nanometer LaCoO
3preparation, by LaCl
3, CoCl
2, NaOH mixes according to the mass ratio of 1:1:6:10, stir into pasty state with the deionized water of 10%, under 500r/min, ball milling 2h obtains precursor matter, by precursor matter at 800 DEG C after roasting 2h, with deionized water wash, after chlorion is cleaned completely, dry 5h for 120 DEG C, obtained nanometer LaCoO
3.
(5) process of rubbish from cooking
The type of A, rubbish from cooking
Described rubbish from cooking is fruits and vegetables class, meat eggs, and mass ratio is 2:1, and water content is 75%, and organic content is 30wt%.
B, enzymolysis
By rubbish from cooking when 85 DEG C of boilings, add the fire resistant alpha-diastase of rubbish from cooking weight 0.03%, the high temperature resistant lipase of rubbish from cooking weight 0.04%, be incubated 3 hours, add the saccharifying enzyme of rubbish from cooking weight 0.02% in 50 DEG C in temperature-fall period, and be incubated 2 hours, add the proteolytic enzyme of rubbish from cooking weight 0.11% and the cellulase of rubbish from cooking weight 0.12% in 37 DEG C and be incubated 2 hours, obtaining enzymolysis mixture.Described enzyme, source is commercially available.
C, press filtration
The solid matter content to 9% of water use regulation enzymolysis mixture, use plate-and-frame filter press to carry out press filtration, working pressure is 0.5MPa, sheet frame is square, and the interior length of side of filter frame is 200 ~ 2000 millimeters, and frame is thick is 16 ~ 80 millimeters, filtration area is 0.5 ~ 1200 square metre, collects filtrate.
D, alkali cleaning
Be the weak caustic solution of 9.5 with pH, add in the filter residue that press filtration obtains, adjustment solid-to-liquid ratio is 1:12, in 40 DEG C of heating in water bath 1h, carries out ultra-sonic oscillation while heating, 20KHZ ultrasonic cleaning 2 minutes, then uses 40KHZ ultrasonic cleaning 1 minute, filters, obtains filtrate.
E, merging filtrate
Filtrate C, D step obtained merges, and obtains total filtrate.
F, sterilizing
By total filtrate of collecting, carry out high-temperature sterilization.
(6) pulverize
Mushroom residue, rice bran, straw powder are pulverized, after pulverizing, order number is 100 orders.
(7) C/N ratio is regulated
Chicken manure, waste molasses, Oleum Folium Artemisiae Argyi, Radix Rhodiolae extract, active rare earth, boric acid, biological humic are mixed, and above-mentioned steps (2), (3), (4), (5), (6) product mix, to mixture regulate C/N be 30:1.
(8) composite fungus agent is sprayed
By complex microbial inoculum, be evenly sprayed in the mixture of step 4.
(9) water content is regulated
Regulate water content to 68wt%.
(10) pile fermentation, prepare fertilizer
Covered with plastic film insulation, moisturizing; pile fermentation is carried out under 30 DEG C ~ 45 DEG C conditions; until pile rotten material in loose crumb structure; pH is 6.2-6.5; namely become thoroughly decomposed complete, be dried to water ratio to below 10wt% with 40 DEG C of warm braws, add trace element, brassinolide, butyric acid potassium; mix the granulation of rear feeding Squeezinggranulator, packaging, obtained nano microbeam microbial inoculum of the present invention.
Result:
(1) the nano microbeam microbial inoculum prepared of the present invention, living bacteria count amount is 6.8-7.2 hundred million/g, total nitrogen content is 8wt%, total phosphorous is 4wt%, total potassium content is 12wt%, and organic (in butt) is 45wt%, water ratio 10wt%, pH is 6.2-6.5, granule strength is 9.2N, and granularity (2-3mm) is 98.6%, and miscellaneous bacteria rate is 3.4%, in butt, total arsenic content≤1.8mg/kg, total cadmium content≤0.4mg/kg, total lead content≤7.5mg/kg, total chrome content≤15mg/kg, total mercury content≤0.1mg/kg.
(2) the nano microbeam microbial inoculum prepared of the present invention, for the plantation of cucumber, output reaches 6520kg/ mu, volume increase 19%, and Vitamin C content is 0.073mg/100g, and increase by 8.6%, total sugar content is 1.43%, increases by 8.9%.
(3) the nano microbeam microbial inoculum prepared of the present invention, for great Jiang plantation, output reaches 1.4 ten thousand pounds/acre, volume increase 25%, and for planting garlic, output reaches 1600kg/ mu, volume increase 18%; For potato plantation, output reaches 2800kg/ mu, volume increase 24%.
(4) the nano microbeam microbial inoculum prepared of the present invention, also has a better role to growing area soil,
After planting one batch of crop, soil organic matter content is 1.7%, and alkali-hydrolyzable nitrogen is 150mg/kg, and available potassium is 160mg/kg, and available phosphorus is 110mg/kg, and soil pH is 6.2, and the soil weight is 1.1g/cm
3, soil porosity is 58%, and Soil Microorganism content is 250 × 10
5individual/g, soil moisture content is 28%, and in soil, organic carbon content is 33g/kg; By the power of 100N, the waddy that diameter is 1cm is inserted soil, the degree of depth that waddy enters soil is 15cm, comparatively uses Common compound fertilizer, and the degree of depth that waddy enters soil increases by 60%.
Before plantation, soil indices is: soil organic matter content is 1.1%, and alkali-hydrolyzable nitrogen is 110mg/kg, and available potassium is 120mg/kg, and available phosphorus is 68mg/kg, and soil pH is 5.6, and the soil weight is 1.5g/cm
3, soil porosity is 41%.
Except as otherwise noted, the percentage ratio adopted in the present invention is weight percentage, and ratio of the present invention, is mass ratio.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a nano microbeam microbial inoculum, is characterized in that: each raw material comprises maltose Nano microsphere, nanometer ground phosphate rock, nanometer LaCoO
3, rubbish from cooking, mushroom residue, rice bran, chicken manure, straw powder, waste molasses, Oleum Folium Artemisiae Argyi, Radix Rhodiolae extract, active rare earth, boric acid, biological humic, trace element fertilizer, brassinolide, butyric acid potassium, complex microbial inoculum.
2. a kind of nano microbeam microbial inoculum according to claim 1, is characterized in that: the mass ratio of each raw material is: maltose Nano microsphere 3 parts, 5 parts, nanometer ground phosphate rock, nanometer LaCoO
34 parts, rubbish from cooking 20 parts, mushroom residue 12 parts, 11 parts, rice bran, chicken manure 20 parts, straw powder 13 parts, waste molasses 5 parts, Oleum Folium Artemisiae Argyi 2 parts, Radix Rhodiolae extract 4 parts, active rare earth 3 parts, boric acid 3 parts, biological humic 10 parts, trace element fertilizer 2 parts, brassinolide 0.1 part, butyric acid potassium 2 parts, complex microbial inoculum 0.3 part.
3. a kind of nano microbeam microbial inoculum according to claim 1, it is characterized in that: described maltose Nano microsphere, volume average particle size is 0.55-0.56 μm, and aerodynamic diameter is 5.334-5.456 μm; Described chicken manure, organic content is 45wt%; Described straw powder, diameter is 2-3mm; Described waste molasses, full sugar is 50wt%, brix 80 degree.
4. a kind of nano microbeam microbial inoculum according to claim 1, is characterized in that: described Oleum Folium Artemisiae Argyi, and the refractive index of 25 DEG C is 1.4650-1.4770, and the relative density of 25 DEG C is 0.89-0.91, and the content of tarragon alcohol is 99.9wt%; Described Radix Rhodiolae extract, powder, moisture <12%, total percent of ash <8%, acid-insoluble ash <2%, total Luo Sai dimension hplc is 5%, and salidroside content is 1%.
5. a kind of nano microbeam microbial inoculum according to claim 1, it is characterized in that: described active rare earth, khaki color powder, REO is 40%, moisture content <0.5%, granularity is 100 orders, and lanthanum content is 12%, cerium content is 10.33%, and praseodymium content is 5.66%, and neodymium content is 3.5%; Described trace element: boric acid, zinc oxide, ferrous sulfate, manganous carbonate, molybdic oxide, mass ratio is 2:3:2:1.
6. a kind of nano microbeam microbial inoculum according to claim 1, it is characterized in that: described complex microbial inoculum, for liquid bacteria suspension, the concentration of its moderate light rhodospirillum is 0.2 hundred million cfu/ml, the concentration of Brasil diazotrophic spirillum is the concentration of 0.3 hundred million cfu/ml, Bai Yelin kirschner bacterium is 0.1 hundred million cfu/ml, the concentration of wood sugar glyconic acid acetobacter is 0.1 hundred million cfu/ml, and the concentration of subtilis is 0.3 hundred million cfu/ml, and the concentration of plant lactobacillus is 0.4 hundred million cfu/ml.
7. a kind of nano microbeam microbial inoculum according to claim 1, it is characterized in that: described nano microbeam microbial inoculum, living bacteria count amount is 6.8-7.2 hundred million/g, total nitrogen content is 8wt%, and total phosphorous is 4wt%, and total potassium content is 12wt%, in butt, organic content is 45wt%.
8. a preparation method for nano microbeam microbial inoculum, is characterized in that: comprise and prepare maltose Nano microsphere, preparation nanometer acidifying ground phosphate rock, preparation nanometer LaCoO
3, rubbish from cooking process, spray composite fungus agent.
9. the preparation method of a kind of nano microbeam microbial inoculum according to claim 8, it is characterized in that: describedly prepare maltose Nano microsphere, get the maltose of 2 weight parts, the polymerization degree of 8 weight parts is the chitosan of 50CPS, be dissolved in the acetic acid solution of 500ml0.6%, mixing, by mixed solution through 0.45 μm of micro-pore-film filtration, obtain filtrate, carry out spraying dry, inlet temperature is 110 DEG C, input speed is 4ml/min, obtains maltose Nano microsphere, and state is powder.
10. the preparation method of a kind of nano microbeam microbial inoculum according to claim 8, is characterized in that: described preparation nanometer LaCoO
3, by LaCl
3, CoCl
2, NaOH mixes according to the mass ratio of 1:1:6:10, stir into pasty state with the deionized water of 10%, under 500r/min, ball milling 2h obtains precursor matter, by precursor matter at 800 DEG C after roasting 2h, with deionized water wash, after chlorion is cleaned completely, dry 5h for 120 DEG C, obtained nanometer LaCoO
3.
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