CN104799043A - Animal-derived waste processing product, and preparation method and application thereof - Google Patents
Animal-derived waste processing product, and preparation method and application thereof Download PDFInfo
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- CN104799043A CN104799043A CN201410040727.2A CN201410040727A CN104799043A CN 104799043 A CN104799043 A CN 104799043A CN 201410040727 A CN201410040727 A CN 201410040727A CN 104799043 A CN104799043 A CN 104799043A
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Abstract
The invention belongs to the technical field of animal processing, and discloses an animal-derived waste processing product, and a preparation method and an application thereof. The preparation method of the animal-derived waste processing product comprises the following steps: obtaining animal-derived wastes comprising animal internal organs; carrying out primary mixing on the animal-derived wastes and water, and homogenizing to obtain a first product; and carrying out secondary mixing on the first product and protease, carrying out enzymatic hydrolysis at 45-50DEG C under a pH value of 6.8-7.2, deactivating, filtering, collecting the obtained filtrate, and drying. The preparation method of the animal-derived waste processing product has the advantages of simple process, and full recycling of the animal-derived wastes, and the animal-derived waste processing product and can be used to prepare feeds or feed additives.
Description
Technical field
The invention belongs to animal processing technique field, particularly a kind of animal sources discarded object converted products, its preparation method and application.
Background technology
Animal-breeding comprises livestock and poultry cultivation, aquaculture and special economic animal cultivation.Livestock and poultry cultivation is mainly the raising of the domestic animals and fowls such as ox, horse, donkey, pig, sheep, chicken, duck, goose, rabbit, and the life for people provides meat, breast, egg, fowl etc. to enrich food.Aquaculture water is the activity in production of breeding, cultivating and gather in the crops aquatic animals and plants under manual control.For animal, the main cultivation object of aquaculture comprises fish, shrimp, Bao, scallop, sea cucumber etc., and the life for people provides the gourmet foods such as fish, shrimp, crab, shellfish.Special economic animal cultivation is the artificial large-scale breeding to special economic animal; special economic animal refers to have specific economic use and economic worth, can provide specialities for the mankind; such as, to meet the wild animal with different domestication degree of the different needs of people, sika deer, Arctic fox, mink etc.Animal-breeding is that people bring huge economic benefit, is one of chief component of agricultural, is listed as the Two Mainstays of agricultural production with plant husbandry.
Along with growth in the living standard, the demand of people to various animal converted products also increases gradually, this facilitates the flourish of animal-breeding and animal secondary industry.Animal form processing also becomes increasingly abundant, such as, and the deep processing of the deep processing of meat product, the deep processing of egg product and milk product.Carrying out animal deep processing, during the deep processing of esp meat product, generally after massacre animal, first removing its internal organ, then carry out further deep processing, this makes, when producing animal converted products, to create a large amount of plucks.Research finds, pluck is rich in the several mineral materials such as iron, zinc, selenium, copper, potassium, calcium, can provide various trace elements for body; Pluck also contains abundant vitamin, such as vitamin A, vitamin B2, vitamin D, vitamin K, folic acid, nicotinic acid alkali etc.These nutriments effectively can supplement body to these trace element and the demand of vitamin.Research also finds, the content of the protein in some pluck can be suitable with the content of protein in meat, but its fat content is more much lower than the content of the fat in meat, has higher nutritive value.But, the internal organ extractd in animal process only have small part, such as pork liver, chicken gizzard, sheep lung, chitling etc. are recovered and utilize the internal organ produced in most animals process to be all simply discarded, and these plucks abandoned constitute animal sources discarded object.Animal sources discarded object does not add utilization and is namely dropped, and causes environmental pollution on the one hand, also result in the waste of resource on the other hand.
At present, the research report about the recycling of animal sources discarded object is less, is badly in need of a kind of animal sources discarded object processing method, to make full use of these resources.
Summary of the invention
In view of this, goal of the invention of the present invention is to provide a kind of animal sources discarded object converted products, its preparation method and application.The preparation technology of this animal sources discarded object converted products is simple, takes full advantage of animal sources discarded object, can be applied to and prepare feed or feed addictive.
In order to realize goal of the invention of the present invention, the present invention adopts following technical scheme:
The invention provides a kind of preparation method of animal sources discarded object converted products, comprising:
Obtain animal sources discarded object, this animal sources discarded object comprises pluck;
Get gained animal sources discarded object to mix with water first, homogenate, obtains the first product;
Get gained first product to mix with protease second, in 45 DEG C ~ 50 DEG C, under the condition of pH6.8 ~ 7.2 after enzymolysis, deactivation, after filtration, collects filtrate, dry, to obtain final product.
Indication animal sources discarded object of the present invention, is included in the pluck abandoned in animal process.Preparation method provided by the invention utilizes protease hydrolyzed animal sources discarded object, and technique is simple, and make use of animal sources waste material fully, gained animal sources discarded object converted products can be applied to prepares feed or feed addictive.
Preferably, in preparation method provided by the invention, pluck is soft-shelled turtle internal organ and/or pig spleen.
Preferably, in preparation method provided by the invention, protease is neutral proteinase.
More preferably, in preparation method provided by the invention, the consumption of neutral proteinase mixes with neutral proteinase described in 500U ~ 5000U for every gram of first product.
In some embodiments of the invention, the enzyme activity of neutral proteinase used in preparation method provided by the invention is 100000U/g.
In other embodiment of the present invention, the mass ratio of the neutral proteinase in preparation method provided by the invention and the first product is 0.5 ~ 5:100.
Preferably, in preparation method provided by the invention, the time of enzymolysis is 2h ~ 5h.
Preferably, in preparation method provided by the invention, deactivation is specially:
Under 90 DEG C ~ 100 DEG C conditions, heating 10min ~ 30min.
Preferably, in preparation method provided by the invention, the mass ratio of animal sources discarded object and water is 1:1, but in preparation method provided by the invention, the mass ratio of animal sources discarded object and water is not restricted to 1:1.
Preferably, in preparation method provided by the invention, the smashing fineness of homogenate is 5 μm ~ 10 μm.
Preferably, in preparation method provided by the invention, in filtration, the aperture of filter membrane is 0.2 μm ~ 5 μm.
Preferably, in preparation method provided by the invention, drying is specially spraying dry.
In some embodiments of the invention, in preparation method provided by the invention, spray-dired temperature is 80 DEG C ~ 90 DEG C.
Present invention also offers a kind of animal sources discarded object converted products, the preparation method of this animal sources discarded object converted products comprises:
Obtain animal sources discarded object, this animal sources discarded object comprises pluck;
Get gained animal sources discarded object to mix with water first, homogenate, obtains the first product;
Get gained first product to mix with protease second, in 45 DEG C ~ 50 DEG C, under the condition of pH6.8 ~ 7.2 after enzymolysis, deactivation, after filtration, collects filtrate, dry, to obtain final product.
Present invention also offers animal sources discarded object converted products and preparing the application in feed or feed addictive, the preparation method of this animal sources discarded object converted products comprises:
Obtain animal sources discarded object, this animal sources discarded object comprises pluck;
Get gained animal sources discarded object to mix with water first, homogenate, obtains the first product;
Get gained first product to mix with protease second, in 45 DEG C ~ 50 DEG C, under the condition of pH6.8 ~ 7.2 after enzymolysis, deactivation, after filtration, collects filtrate, dry, to obtain final product.
Present invention also offers a kind of feed, it comprises animal sources discarded object converted products provided by the invention, and the preparation method of this animal sources discarded object converted products comprises:
Obtain animal sources discarded object, this animal sources discarded object comprises pluck;
Get gained animal sources discarded object to mix with water first, homogenate, obtains the first product;
Get gained first product to mix with protease second, in 45 DEG C ~ 50 DEG C, under the condition of pH6.8 ~ 7.2 after enzymolysis, deactivation, after filtration, collects filtrate, dry, to obtain final product.
In some embodiments of the invention, feed provided by the invention is specially chicken feed.
Preferably, in feed provided by the invention, by percentage to the quality, the content of this animal sources discarded object converted products is 0.1% ~ 0.99%.
The invention provides a kind of animal sources discarded object converted products, its preparation method and application.The preparation method of this animal sources discarded object converted products comprises: obtain animal sources discarded object, this animal sources discarded object comprises pluck; Get gained animal sources discarded object to mix with water first, homogenate, obtains the first product; Get gained first product to mix with protease second, in 45 DEG C ~ 50 DEG C, under the condition of pH6.8 ~ 7.2 after enzymolysis, deactivation, after filtration, collects filtrate, dry, to obtain final product.The preparation method of animal sources discarded object converted products provided by the invention adopts biology enzyme enzymolysis animal sources discarded object, and preparation technology is simple, has fully recycled animal sources discarded object.Experimental result shows, this animal sources discarded object converted products effectively can improve feed quality, significantly improves animal growth rate, can be applied to and prepare feed or feed addictive.
Accompanying drawing explanation
Fig. 1 shows the ultra-violet absorption spectrum of the animal sources discarded object converted products that the embodiment of the present invention 1 is obtained, and wherein the wavelength of 1 correspondence is 235.20nm, Abs is 0.101; 2 is the ultraviolet absorption peak of the obtained animal sources discarded object converted products of embodiment 1, and corresponding wavelength is 250.40nm, Abs is 0.142;
Fig. 2 shows the ultra-violet absorption spectrum of the animal sources discarded object converted products that the embodiment of the present invention 2 is obtained, and wherein the wavelength of 1 correspondence is 235.20nm, Abs is 0.100; 2 is the ultraviolet absorption peak of the obtained animal sources discarded object converted products of embodiment 1, and corresponding wavelength is 250.60nm, Abs is 0.141;
Fig. 3 shows the ultra-violet absorption spectrum of the animal sources discarded object converted products that the embodiment of the present invention 3 is obtained, and wherein the wavelength of 1 correspondence is 235.60nm, Abs is 0.101; 2 is the ultraviolet absorption peak of the obtained animal sources discarded object converted products of embodiment 1, and corresponding wavelength is 250.60nm, Abs is 0.141;
Fig. 4 shows that the Zhou Zengchong of the young cock of different group in the embodiment of the present invention 4 changes meander line, wherein 1 be the young cock of experimental group 3 Zhou Zengchong change meander line, wherein 2 be the young cock of experimental group 2 Zhou Zengchong change meander line, wherein 3 be the young cock of experimental group 1 Zhou Zengchong change meander line, wherein 4 be the young cock of control group Zhou Zengchong change meander line.
Detailed description of the invention
The invention discloses a kind of animal sources discarded object converted products, its preparation method and application.Those skilled in the art can with reference to present disclosure, obtain this animal sources discarded object converted products, realize its application, special needs to be pointed out is, all similar replacements and change apparent to those skilled in the art, they are all deemed to be included in the present invention.Preparation method of the present invention and application are described by preferred embodiment, related personnel obviously can not depart from content of the present invention, spirit and scope this paper preparation method and application are changed or suitably change with combination, realize and apply the technology of the present invention.
Reagent used in a kind of animal sources discarded object converted products provided by the invention, its preparation method and application and raw material all can be buied by market.
In order to enable those skilled in the art understand technical scheme of the present invention better, below in conjunction with embodiment, set forth the present invention further:
The preparation of embodiment 1 animal sources discarded object converted products
Collect the soft-shelled turtle internal organ 5kg abandoned in soft-shelled turtle process, mix with the water of 5kg, it is 5 μm that homogenate is crushed to fineness, (neutral proteinase is bought in Zhengzhou Tian Hua food additives Co., Ltd to add neutral proteinase 50g, enzyme activity is 100000U/g), mix, pour in enzyme digestion reaction tank and carry out enzymolysis, hydrolysis temperature is 50 DEG C, pH6.8.Enzymolysis 5 hours, enzymolysis terminates, and is heated to 90 DEG C, after insulation 30min, with 0.2 μm of membrane filtration, collects filtrate, by spraying dry at gained filtrate 80 DEG C, obtains powder 1.07kg, obtain animal sources discarded object converted products 1.07kg.
The animal sources discarded object converted products obtained to the present embodiment carries out Components identification.Adopt Forint phenol method detection content of peptides wherein, concrete operations are as follows:
Reagent:
Alkaline copper test solution: get NaOH 10g, sodium carbonate 50g, the 400ml that adds water makes dissolving, as first liquid; Get potassium tartrate 0.5g, the 50ml that adds water makes it dissolve, and separately gets copper sulphate 0.25g, and the 30ml that adds water makes it dissolve, and gained tartaric acid aqueous solutions of potassium and copper sulfate solution is carried out mixing as second liquid.Before use, merge first liquid and second liquid, and add water to 500mL, obtain alkaline copper reagent.
The preparation of calibration curve: get bovine serum albumin product in contrast, add water and be made into the solution of 0.3mg/mL.Precision measure reference substance solution 0.0,0.1,0.3,0.5,0.7,0.9mL, put respectively in tool plug test tube, respectively add water to 1.0mL, mark one by one, add alkaline copper test solution 1.0mL more respectively, shake up, respectively add forint phenol test solution (getting 1.0mol/L forint test solution 1 → 8) 4.0mL, mix immediately, put in 55 DEG C of water-baths and react 5 minutes, put cold bath 10 minutes, according to spectrophotometry operation sequence, measure trap at 650nm wavelength place; Using the Reagent Tube of 0.0 correspondence as blank.Regression equation is calculated with reference substance solution concentration and corresponding absorbance.
Determination method: precision measures the obtained animal sources discarded object converted products 2.5mg of the present embodiment, add the water-soluble solution of 10mL, get 1.0mL gained solution and add alkaline copper test solution 1.0mL, then add alkaline copper test solution 1.0mL respectively, shake up, respectively add above-mentioned forint phenol test solution (getting 1.0mol/L forint test solution 1 → 8) 4.0mL, mix immediately, put in 55 DEG C of water-baths and react 5 minutes, put cold bath 10 minutes, according to spectrophotometry operation sequence, measure absorbance at 650nm wavelength place.According to the content of the regression equation calculation polypeptide that the present embodiment records, and be multiplied by extension rate, obtain final product.
Detected by above-mentioned Forint phenol method, the content recording polypeptide in the animal sources discarded object converted products that the present embodiment obtains is 410mg/g.
Get the animal sources discarded object converted products 2.5mg that the present embodiment is obtained, add the water-soluble solution of 10mL, scanned by gained solution ultraviolet specrophotometer, scanning wavelength interval is 200nm ~ 300nm, investigates its absorption maximum peak-to-peak value.Testing result is shown in Fig. 1, records its maximum absorption band at 250.40nm place.
The preparation of embodiment 2 animal sources discarded object converted products
Collect pig spleen 5kg, mix with the water of 5kg, it is 10 μm that homogenate is crushed to fineness, (neutral proteinase is bought in Zhengzhou Tian Hua food additives Co., Ltd to add neutral proteinase 500g, enzyme activity is 100000U/g), mix, pour in enzyme digestion reaction tank and carry out enzymolysis.Hydrolysis temperature is 45 DEG C, pH7.2.Enzymolysis 2 hours, enzymolysis terminates, and is heated to 100 DEG C, after insulation 10min, with 0.5 μm of membrane filtration, collects filtrate, by spraying dry at gained filtrate 90 DEG C, obtains powder 1.25kg, obtain animal sources discarded object converted products 1.25kg.
The animal sources discarded object converted products obtained to the present embodiment carries out Components identification.Adopt Forint phenol method detection content of peptides wherein, the detection method that concrete operation method provides as embodiment 1.Detected by Forint phenol method, the content recording polypeptide in the animal sources discarded object converted products that the present embodiment obtains is 435mg/g.
Get the animal sources discarded object converted products 2.5mg that the present embodiment is obtained, add the water-soluble solution of 10mL, scanned by gained solution ultraviolet specrophotometer, scanning wavelength interval is 200nm ~ 300nm, investigates its absorption maximum peak-to-peak value.Testing result is shown in Fig. 2, records its maximum absorption band at 250.60nm place.
The preparation of embodiment 3 animal sources discarded object converted products
Collect pig spleen 2.5kg, soft-shelled turtle internal organ 2.5kg, mix with the water of 5kg, it is 8 μm that homogenate is crushed to fineness, (neutral proteinase is bought in Zhengzhou Tian Hua food additives Co., Ltd to add neutral proteinase 300g, enzyme activity is 100000U/g), mix, pour in enzyme digestion reaction tank and carry out enzymolysis.Hydrolysis temperature is 48 DEG C, pH7.0.Enzymolysis 4 hours, enzymolysis terminates, and is heated to 95 DEG C, after insulation 20min, with 5 μm of membrane filtrations, collects filtrate, by spraying dry at gained filtrate 85 DEG C, obtains powder 1.15kg, obtain animal sources discarded object converted products 1.15kg.
The animal sources discarded object converted products obtained to the present embodiment carries out Components identification.Adopt Forint phenol method detection content of peptides wherein, the detection method that concrete operation method provides as embodiment 1.Detected by Forint phenol method, the content recording polypeptide in the animal sources discarded object converted products that the present embodiment obtains is 419mg/g.
Get the animal sources discarded object converted products 2.5mg that the present embodiment is obtained, add the water-soluble solution of 10mL, scanned by gained solution ultraviolet specrophotometer, scanning wavelength interval is 200nm ~ 300nm, investigates its absorption maximum peak-to-peak value.Testing result is shown in Fig. 3, records its maximum absorption band at 250.60nm place.
Embodiment 4 animal sources discarded object converted products is on the impact of chicken growth rate
The animal sources discarded object converted products ultraviolet specrophotometer that Example obtains scans, and scanning wavelength interval is 200nm ~ 300nm, and maximum absorption band, between 249nm ~ 252nm, can promote for containing the active material that young cock grows.
The animal sources discarded object converted products that Example 1 obtains directly adds in common chicken feed, investigates animal sources discarded object converted products provided by the invention to the impact of chicken growth rate according to normal method for breeding.
Choose the healthy young cock 180 of 1 age in days, raise, feed is not containing the SPF chick material of any growth hormone, when raising to 7 age in days, choose the young cock that 120 sizableness body weight are basically identical, be divided into 4 groups at random, often organize 30, be labeled as control group, experimental group 1, experimental group 2 and experimental group 3 respectively.Control group feeds the SPF chick material not adding the obtained animal sources discarded object converted products of embodiment 1.Experimental group feed with the addition of the obtained animal sources discarded object converted products of embodiment 1 raise SPF chick material: by percentage to the quality, experimental group 1 feeds the SPF chick material of the obtained animal sources discarded object converted products of interpolation 0.1% embodiment 1, and namely in the feed that provides of experimental group 1, the mass percentage content of the animal sources discarded object converted products that embodiment 1 is obtained is 0.1%; Experimental group 2 feeds the SPF chick material of the obtained animal sources discarded object converted products of interpolation 0.5% embodiment 1, and namely in the feed that provides of experimental group 2, the mass percentage content of the animal sources discarded object converted products that embodiment 1 is obtained is 0.5%; Experimental group 3 feeds the SPF chick material of the obtained animal sources discarded object converted products of interpolation 1% embodiment 1, and namely in the feed that provides of experimental group 3, the mass percentage content of the animal sources discarded object converted products that embodiment 1 is obtained is 0.99%.Experimental group 1, experimental group 2, experimental group 3 are identical with control group rearing conditions, all strictly raise at clean animal house, free choice feeding, personal management.Clinical observation duration of test day by day observed and recorded respectively organize chicken activity, search for food and health status.Within every 7 days, weigh once, with SPSS16 software, statistical analysis is carried out to Gain weight, represent by mean+SD.All Gain weights of the young cock of each group are in table 1.With body weight and mapping in age in week, the Zhou Zengchong obtaining the young cock of different group changes meander line, i.e. Fig. 4.
The body weight growth pattern of the young cock of the different group of table 1
As can be seen from table 1 and Fig. 4, from 5 week age, the weight gain of experimental group 1, experimental group 2 and experimental group 3 is apparently higher than control group, during 6 week age, experimental group 1, experimental group 2 and experimental group 3 respectively compared with control group, weight gain difference extremely significantly (P<0.01), especially with experimental group 2 and experimental group 3 more obvious, experimental group 1, experimental group 2 is compared with experimental group 3, the increase of the animal sources discarded object converted products content obtained along with the embodiment of the present invention in feed for nursing 1, the amount that the young cock body weight of different group increases also increases gradually, the animal sources discarded object converted products that the embodiment 1 describing interpolation 0.1% ~ 1% in feed obtains (namely by percentage to the quality, in feed, the content of the animal sources discarded object converted products that embodiment 1 is obtained is 0.1% ~ 0.99%) significantly can promote the growth of chicken, the rate of rise of effective raising chicken, and this effect increases along with the increase of animal sources discarded object converted products content.So, the embodiment of the present invention 1 obtained animal sources discarded object converted products can effectively improve growth of animal speed, can be applied to and prepare feed or feed addictive.
Adopt identical experimental technique to investigate embodiment 2 and embodiment 3 make animal sources discarded object converted products is on the impact of chicken growth rate, obtain similar experimental result, illustrate to add in feed 0.1% ~ 1% embodiment 2 or the obtained animal sources discarded object converted products of embodiment 3 (namely by percentage to the quality, in feed, the content of embodiment 2 or the obtained animal sources discarded object converted products of embodiment 3 is 0.1% ~ 0.99%) significantly can promote the growth of chicken, the rate of rise of effective raising chicken, and this effect increases along with the increase of animal sources discarded object converted products content.So the animal sources discarded object converted products that the embodiment of the present invention 2 or embodiment 3 obtain can effectively improve growth of animal speed, can be applied to and prepare feed or feed addictive.
Below be only the preferred embodiment of the present invention, it should be pointed out that above-mentioned preferred embodiment should not be considered as limitation of the present invention, protection scope of the present invention should be as the criterion with claim limited range.For those skilled in the art, without departing from the spirit and scope of the present invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. a preparation method for animal sources discarded object converted products, is characterized in that, comprising:
Obtain animal sources discarded object, described animal sources discarded object comprises pluck;
Get described animal sources discarded object to mix with water first, homogenate, obtains the first product;
Get described first product to mix with protease second, in 45 DEG C ~ 50 DEG C, under the condition of pH6.8 ~ 7.2 after enzymolysis, deactivation, after filtration, collects filtrate, dry, to obtain final product.
2. preparation method according to claim 1, is characterized in that, described pluck is soft-shelled turtle internal organ and/or pig spleen.
3. preparation method according to claim 1, is characterized in that, described protease is neutral proteinase.
4. preparation method according to claim 3, is characterized in that, the consumption of described neutral proteinase mixes with neutral proteinase described in 500U ~ 5000U for every gram of described first product.
5. preparation method according to claim 1, is characterized in that, the time of described enzymolysis is 2h ~ 5h.
6. preparation method according to claim 1, is characterized in that, described deactivation is specially:
Under 90 DEG C ~ 100 DEG C conditions, heating 10min ~ 30min.
7. the animal sources discarded object converted products that the preparation method as described in claim 1 to 6 any one obtains.
8. animal sources discarded object converted products as claimed in claim 7 is preparing the application in feed or feed addictive.
9. a feed, is characterized in that, it comprises animal sources discarded object converted products as claimed in claim 7.
10. feed according to claim 9, is characterized in that, by percentage to the quality, the content of described animal sources discarded object converted products is 0.1% ~ 0.99%.
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