CN103621794A - Method of preventing livestock and poultry pollution by adopting protease produced by food processing leftover material - Google Patents
Method of preventing livestock and poultry pollution by adopting protease produced by food processing leftover material Download PDFInfo
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- CN103621794A CN103621794A CN201310689781.5A CN201310689781A CN103621794A CN 103621794 A CN103621794 A CN 103621794A CN 201310689781 A CN201310689781 A CN 201310689781A CN 103621794 A CN103621794 A CN 103621794A
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Abstract
The invention discloses a method of preventing livestock and poultry pollution by adopting protease produced by a food processing leftover material. The method comprises the following steps of: crushing the food processing leftover material into paste, filling the paste into a fermentation tank with microbial strains and fermenting to prepare a mucous enzyme preparation; mixing a dry matter with the enzyme preparation in a ratio of 1:1, fermenting for 48 hours at 38 DEG C, drying at the temperature lower than 50 DEG C, crushing into 60-mesh powder, and packaging to obtain a finished product. Viable count in the finished product protease reaches 2*10<9> cpu/g, and protein content can be improved by 20% in comparison with the prior art. 500g of the protease disclosed by the invention is mixed with 1 ton of complete feed by adding water in the ratio of 1:1 for fermenting for 12-24 hours at the normal temperature; the obtained product is used for feeding the livestock and poultry, so that livestock and poultry excrement is not smelly; a great deal of the added probiotics is contained in the excrement, so that the excrement can be quickly degraded, and nutrition in the complete feed can be converted into mycoprotein for being sufficiently absorbed by the livestock and poultry; a few nutrients are contained in the excrement, so that the excrement can be used as a colony house fermentation bed padding material, and the excessive padding is sold as nutrient soil, therefore, zero exhaustion of the livestock and poultry excrement is achieved.
Description
Technical field
The present invention relates to food processing leftover pieces treatment technology, protease production technology, the fields such as livestock and poultry cultivation technology, feed processing technology, environmental pollution prevention and control technology, are specifically related to adopt food processing leftover pieces to produce the method that protease control livestock and poultry pollute.
Background technology
When pre-treatment food processing leftover pieces method is mainly that producer processes at a low price, government concentrates heat sterilization, and fermentation produces marsh gas power generation, the discharge of natural pond liquid, and natural pond slag is made fertilizer.It is also to produce marsh gas power generation to process that livestock and poultry cultivation ight soil is processed, and equipment investment cost is high, profit is low, and livestock and poultry colony house microecosystem is difficult to set up by feces of livestock and poultry oneself, needs additional fermentation bed probiotics to complete, and cost is very high.
Summary of the invention
The present invention is directed to the problems referred to above, utilized the feature of the easy breed bacteria of food processing leftover pieces, added current national to allow the probio of using, use Chinese patent CN200810011304.2 bacterium process processed quantitative certainly to the advantage degree of depth poisonous and harmful substances such as killing virus in food processing leftover pieces, pathogenic bacteria of fermenting with probio, food processing leftover pieces are changed into another material liquid enzyme preparation, enzyme preparation and protein feed dregs of beans anaerobic fermentation, the number of viable that generates protease reaches 2.0X10
9cpu/g(spectrum Buddhist nun tests Report ID:H11181038508D), protein content brings up to 51%.Protease can be made eliminating smell agent, probio bacterial classification is used, especially can be used as feed addictive, reach and save food, produce green organic livestock and poultry, control feces of livestock and poultry contaminated environment, adopts micro-ecology fermentation technology to process food processing leftover pieces, produce protease feed addictive cultivation green organic livestock and poultry, the method for control livestock and poultry contaminated environment.
Employing food processing leftover pieces of the present invention are produced the method that protease control livestock and poultry pollute, and its concrete steps are as follows:
(S1) prepare bacterial classification: bacterial classification be VREF, Lactobacillus acidophilus, bacillus subtilis, bafillus natto, bacillus coagulans one or more;
(S2) fermentation: food processing leftover pieces are pulverized and broken into pasty state, pack into and contain one or more fermentation vat of VREF, Lactobacillus acidophilus, bacillus subtilis, bafillus natto, bacillus coagulans and ferment, when pH value reaches 3-5, by wet feed crusher in crushing, become pasty state, continue degree of depth fermentation, when pH value 2.5-3, upper strata is grease, middle level is mucus, and lower floor is pastel, and described mucus is enzyme preparation;
(S3) dry is mixed by 1:1 with the enzyme preparation that step (S2) obtains, 38 ℃ ferment 48 hours, and 50 ℃ of following oven dry, are ground into 60 order powder, packed products.
Wherein, food processing leftover pieces are one or more mixing such as Bread and Cakes processing leftover pieces, meat products processing leftover pieces, fruit processing leftover pieces, processing of aquatic products leftover pieces.Dry is one or more mixing such as pine needle meal, Leaves of Corylus heterophylla Fish. ex Bess powder, corn stalk powder, alfalfa meal, ostreae testa pulverata, dried small shrimp powder, crab shell powder, dregs of beans, wheat bran.
After qualified by forage health standard detection, be packaged as finished product protease, in this finished product protease, viable count can reach 2.0X10 after testing
9cpu/g, crude protein content can reach 51%, with 500 grams, this protease, mix 1 ton of complete feed and add water 1:1(1 ton feed, one ton of water, 500 grams of enzyme preparations) normal temperature fermentation 12-24 hour, the livestock and poultry of feeding, feces of livestock and poultry is not smelly, in ight soil, there are a large amount of added probios, ight soil is degraded very soon, in complete feed, nutrients help changes into mycoprotein and easily by livestock and poultry, is fully absorbed, in ight soil, nutrients seldom, ight soil can be done colony house fermenting bed padding, and unnecessary bedding and padding become Nutrition Soil to be sold, thereby reaches feces of livestock and poultry zero-emission.
The invention has the beneficial effects as follows:
1, the viable count in finished product protease reaches 2 * 10
9cpu/g, and protein content can be brought up to 20% compared with prior art;
2, production cost is low, 5 yuan/kilogram of protease production costs;
3, the present invention carries out harmless treatment to food processing leftover pieces, has saved grain, has improved environmental quality;
4, the crude protein in feed is changed into mycoprotein by probio, and the edible such feed of livestock and poultry can absorb nutritional labeling wherein fully, saves feed, improved the utilization rate of feed 10%-15%, for example, in wheat bran, crude protein content is 15% simultaneously, by fermentation, bring up to 19%; So just can reduce the grain cost of livestock and poultry 20%-30% every day, for example, the dregs of beans of former day grain protein 40%, the protein content after fermentation brings up to 51%, reduces greatly the consumption of grain; Wherein, it is with reference to the < < enzyme bean pulp fermentation of Shandong light industry Academy publication, to produce the research > > of high protein enzyme living feed that bean pulp fermentation is produced protease, improves.
5, the edible livestock and poultry that contain protease feed, are difficult for sickly, do not need to use antibiotic etc, and therefore, the product of the Production of Livestock and Poultry of raising is like this production green organic food; Wherein, the feed of preparing meets the relevant protease and No. 1126, formula feed The Ministry of Agriculture of the People's Republic of China, MOA bulletin and The Ministry of Agriculture of the People's Republic of China, MOA produced in feed and feed addictive management rules, livestock and poultry breeding scale pollution prevention ordinance, Chinese patent 201210308608.1, Chinese patent 201010209663.6 and Chinese patent ZL201010143992.5 and announces the pertinent regulations of No. 1867;
6, produce protease and can do lactic acid bacteria culturers, eliminating smell agent, house refuse degradation agent etc.
The specific embodiment
Preparation VREF, Lactobacillus acidophilus, bacillus subtilis, bafillus natto, bacillus coagulans, bacterium process processed can select the bacterial classification cultural method of setting forth in Chinese patent CN200810011304.2 to realize.
Embodiment 1
The present invention adopts micro-ecology fermentation technology that food processing leftover pieces are processed into enzyme preparation, adds dry to make protease make feed addictive and raise the prevent and remedy pollution method of environment of livestock and poultry, comprise the steps: with liquid enzyme preparation
1, food processing Bread and Cakes leftover pieces are pulverized after breaking into pasty state and packed into the enzyme preparation that ox mouth shape liquid is made in the fermentation of bacillus natto to ferment tank depth, by feed hygiene index, detect up to standard rear standby.
2, dregs of beans is added to enzyme preparation by 1:1, 38 ℃ of fermentations after 48 hours 50 ℃ of following low temperature drying powder become 60 order powder, press sanitary index bacterial detection sum for feed, crude protein content, enzyme activity, miscellaneous bacteria sum, heavy metal arsenic mg/kg (in As) ﹤ 5.0, plumbous mg/kg(is in Pb)≤10.0, cadmium mg/kg(is in Cd)≤0.5, AFB i mg/kg≤0.05, salmonella mpn/100g≤3000(notes: must not detect medicine and material that country's order is forbidden), detect qualified packed products, with reference to bean pulp fermentation, produce the research of high protein enzyme living feed.
Embodiment 2
The present invention is processed into protease by processing meat product leftover pieces and is made feed addictive and raise the prevent and remedy pollution method of environment of livestock and poultry, comprises the steps:
By the leftover pieces in meat products process, broken bone, meat mincing, expired food add and are broken into 20 orders after ripe and pack the fermentation vat degree of depth fermentation that adds Lactobacillus acidophilus into, with national forage standard, detect qualified rear Separation of Solid and Liquid, liquid adds wheat bran, regulates humidity at 50%-60%, 38 ℃ of fermentations of anaerobism add wheat bran by 1:1 after 48 hours again, 50 ℃ of following oven dry of low temperature, are ground into 60 order powder, after protease detection is qualified, package spare.
Embodiment 3
The present invention is the leftover pieces in fruit process, and the organic matters such as fallen fruits, pericarp, dish leaf are processed into protease to be made feed addictive and raise the prevent and remedy pollution method of environment of livestock and poultry and comprise the steps:
By the leftover pieces in fruit process, fallen fruits, pericarp, the organic matter fragmentations such as dish leaf pack the fermentation vat degree of depth fermentation that adds VREF into, after detection is qualified, with the liquid 1:1 that becomes the wire drawing of ox saliva shape, add wheat bran 38 ℃ of the anaerobism fermentation 48 hours that stirs, with what ferment, in 1:1 ratio, add wheat bran to mix all again, 50 ℃ of following temperature are dried, be ground into 60 order powdered protein enzymes, detect after qualified and package spare, can be used as rubbish eliminating smell agent, garbage degradation powder, changing food waste leavening, lactic acid bacteria culturers, a series of products such as toilet deodorant, feature is that cost is low, every 500 grams one yuan more, particularly being used for processing house refuse can degrade organic matter to change into water very soon, reduce volume, reduce environmental pollution.
Embodiment 4
The present invention is organic matters such as processing of aquatic products leftover pieces, is processed into protease and as feed addictive, raises the prevent and remedy pollution method of environment of livestock and poultry and comprise the steps:
By the leftover pieces in processing of aquatic products, marine alga, oyster, small fish, crab shell, dried small shrimp, Deng organic matter fragmentation, pack the fermentation vat degree of depth fermentation that adds bacillus coagulans into, detect the sanitary index that its index of pH3-4 reaches enzyme Preparations Used for Feeds, become the liquid of ox saliva shape wire drawing, by 1:1, add powder to become 40 object pine needle meals, Leaves of Corylus heterophylla Fish. ex Bess powder, corn stalk powder, alfalfa meal, ostreae testa pulverata, dried small shrimp powder, one or more of crab shell powder contain the required dry of various micro-livestock and poultry, after being stirred 38 ℃ of anaerobic fermentations and adding above-mentioned dry to dry in 1:1 ratio again after 48 hours, be ground into 40 order powdered protein enzymes, detect after qualified and package spare.
Embodiment 5
The leftovers leftovers that the present invention is not subject to secondary pollution by food and drink add fermentation of bacillus subtilis locally, transport to after processing factory and shatter and joined degree of depth fermentation in lactobacillus acidophilus fermentation vat, with ox mouth hydratoid, this adds 38 ℃ of fermentations of dregs of beans anaerobism, 50 ℃ of following oven dry after 48 hours by 1:1, and it is standby that powder becomes 60 order powder to make protease.
To in the surplus materials in above-described embodiment, add other material damping degree 50% such as brewex's grains---60% mixes all oven dry or Direct-fed livestock and poultry.
Protease in above-described embodiment is mixed and in 1 ton of livestock and poultry diet, added water and within 12-24 hour, feed by 1:1 normal temperature fermentation by 500 gram, can save grain, reduce daily ration cost, livestock and poultry good absorbing, ight soil moulding is not smelly, ight soil more than 10 ℃ can be degraded within 12 hours, in ight soil, there are a large amount of added probios, thereby reach the object of control feces of livestock and poultry contaminated environment, with this protease, raise pigs and solved 300 jin of thick problems of above pig back fat, pig can be supported 500 days, the black pig of ternary can grow to 700 jin, back fat is below 3 centimeters, dressing percentage 70%, lean meat percentage 70%, yellowish pink dark red, marbling is obvious, fat content 8% left and right between flesh, meat flavour is special aromatic, protein, amino acid, between flesh, fat content is high, cholesterol is low, owing to adopting, food processing leftover pieces feeding cost is low, with protease livestock birds health, do not fall ill, immunity need not be had an injection by force, improve income.
In addition the probio that, the present invention adds is one or more of VREF, Lactobacillus acidophilus, bacillus subtilis, bafillus natto, bacillus coagulans.When adding multiple probio, along with the not square one of difference and the bacterium amount of later stage fermentation condition, one or more limited probios that often only survive are for fermentation.That is to say, exist bacterial classification that part adds because of the nonviable situation of ambient conditions, and bacterial classification for fermenting is all to meet life condition or have quantitative advantage.
The above; it is only the preferably specific embodiment of the present invention; but protection scope of the present invention is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; according to technical scheme of the present invention and inventive concept thereof, be equal to replacement or changed, within all should being encompassed in protection scope of the present invention.
Claims (5)
1. adopt food processing leftover pieces to produce the method that protease control livestock and poultry pollute, it is characterized in that following steps:
S1, prepare bacterial classification: bacterial classification is one or more of VREF, Lactobacillus acidophilus, bacillus subtilis, bafillus natto, bacillus coagulans;
S2, fermentation: by food processing leftover pieces, pack into and contain one or more fermentation vat of VREF, Lactobacillus acidophilus, bacillus subtilis, bafillus natto, bacillus coagulans and carry out prior fermentation, when pH value reaches 3-5, be broken into pasty state, continue degree of depth fermentation, when pH value 2.5-3, upper strata is grease, middle level is mucus, and lower floor is pastel, and described middle level mucus is enzyme preparation;
S3, dry is mixed by 1:1 with the enzyme preparation that step (S2) obtains, 38 ℃ of fermentations 48 hours, 50 ℃ of following oven dry, are ground into 60 order powder, packed products.
2. adopt according to claim 1 food processing leftover pieces to produce the method that protease control livestock and poultry pollute, it is characterized in that, described food processing leftover pieces are Bread and Cakes processing leftover pieces, meat products processing leftover pieces, fruit processing leftover pieces, one or more mixing of processing of aquatic products leftover pieces.
3. according to adopting food processing leftover pieces to produce the method that protease control livestock and poultry pollute described in claim 1 or 2, it is characterized in that, described dry is one or more mixing of pine needle meal, Leaves of Corylus heterophylla Fish. ex Bess powder, corn stalk powder, alfalfa meal, ostreae testa pulverata, dried small shrimp powder, crab shell powder, dregs of beans, wheat bran.
4. according to the arbitrary described employing food processing leftover pieces of claim 1-3, produce the method that protease control livestock and poultry pollute, it is characterized in that, step S2 mid-early stage the food processing leftover pieces that ferments through pulverize break into pasty state after for fermenting.
5. according to the arbitrary described employing food processing leftover pieces of claim 1-3, produce the method that protease control livestock and poultry pollute, it is characterized in that, with 500 grams, this protease, mix 1 ton of complete feed and add water 1:1, normal temperature fermentation 12-24 hour, the livestock and poultry of feeding, feces of livestock and poultry is not smelly, in ight soil, there are a large amount of added probios, ight soil is degraded very soon, in complete feed, nutrients help changes into mycoprotein and easily by livestock and poultry, is fully absorbed, and in ight soil, seldom, ight soil can be done colony house fermenting bed padding to nutrients, unnecessary bedding and padding become Nutrition Soil to be sold, thereby reaches feces of livestock and poultry zero-emission.
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Cited By (5)
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| CN104642727A (en) * | 2015-01-28 | 2015-05-27 | 罗均宏 | Microbial fermented high-protein feed and preparation method thereof |
| CN105901425A (en) * | 2016-04-25 | 2016-08-31 | 固镇县益民养殖专业合作社 | Feed for preventing and controlling sinergasilliasis of Aristichthys nobilis |
| CN108668915A (en) * | 2018-03-31 | 2018-10-19 | 潍坊友容实业有限公司 | A kind of salt-soda soil livestock and poultry cultivation fermenting bed padding additive and preparation method thereof |
| CN111690560A (en) * | 2020-06-10 | 2020-09-22 | 青岛玛斯特生物技术有限公司 | Enterococcus faecium and application thereof in aquaculture |
| CN115918777A (en) * | 2022-12-23 | 2023-04-07 | 福建师范大学 | Method for treating fruit and vegetable garbage by using microbial agent |
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Application publication date: 20140312 |