CN105010724A - Microbial agent and straw organic feed produced through microbial agent - Google Patents
Microbial agent and straw organic feed produced through microbial agent Download PDFInfo
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- CN105010724A CN105010724A CN201410181933.5A CN201410181933A CN105010724A CN 105010724 A CN105010724 A CN 105010724A CN 201410181933 A CN201410181933 A CN 201410181933A CN 105010724 A CN105010724 A CN 105010724A
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Abstract
The invention relates to a microbial agent and straw organic feed produced through the microbial agent. A proper quantity of fermentation strains are added into an aqueous solution with herba lycopi and kobold flowers being smashed, so that a microorganism leavening agent is prepared; smashed waste mushroom residues obtained after straw is used as a raw material to produce edible mushrooms are mixed with the microorganism leavening agent according to the weight part proportion of 9 to 1, sterilization, fermentation, drying and pelleting are sequentially conducted on the material after the moisture content is adjusted, and accordingly a microbial agent particle product is obtained. The microbial agent is mixed with straw powder according to the weight part proportion of 1 to 19, sterilization, fermentation, drying and pelleting are sequentially conducted on the material after the moisture content is adjusted, and accordingly a crude fiber straw organic feed product is obtained. The microbial agent is mixed with waste mushroom residue powder obtained after straw is used as the raw material to produce the edible mushrooms according to the weight part proportion of 1 to 19, sterilization, fermentation, drying and pelleting are sequentially conducted on the material after the moisture content is adjusted, and accordingly an edible mushroom waste mushroom residue straw organic feed product is obtained.
Description
Technical field
The invention belongs to the preparing technical field of biochemical article, the stalk organic feed relating to a kind of straw degradative agent and utilize this degradation agent to produce.
Background technology
Along with the raising of China's farm output, the output of agricultural crop straw (as corn stalk, wheat straw, sorghum stalks, peanut shell, peanut stalk, rice bran, straw, leaf etc.) is also being multiplied.Made according to the relevent statistics, the output of the current all kinds of agricultural crop straw in the whole nation is every year more than 600,000,000 tons, and its quantity is equivalent to 60 times of Northern grassland output.For a long time, the process problem of agricultural crop straw is the problem making vast farmers and governments at all levels feel more headache always, past is due to reasons such as the shortage of evaluation and exploration technology, market information are inaccessible and peasant's autumn ploughing sowing time is of short duration, most (70% ~ 80%) stalk goes out of use in river course, rot or directly destroyed by fire in field in roadside, not only block river course and road traffic, and severe contamination environment cause the huge waste of resource.In view of above-mentioned present situation, in recent years, how to develop stalk is the important topic that those skilled in the art are devoted to study always, and be the development that raw material makes edible mushroom technology (cultivating the edible mushroom technology such as pleurotus eryngii, flat mushroom, auricularia auriculajudae, mushroom, straw mushroom, Ji mushroom, asparagus, Agricus blazei by after crushed stalk fermentation) with stalk, the exploitation for stalk provides an effective method.But owing to utilizing stalk for containing many nutriments in bacterium slag discarded after raw material production edible mushroom, these nutriments can not be utilized in time, mosquitos and flies is caused to grow, stench spreads, and causes great pollution, directly have influence on the living environment of people in discarded bacterium bag place of arrival.In order to address this problem, those skilled in the art once adopted pulverizing, directly returned the mode process bacterium slag of field or spontaneous fermentation, although result can obtain more satisfactory result originally year, but from Second Year, fungus strain due to waste bacterial slag affects the normal breeding of plant fungus strain, cause abnormal growth and the disease of normal plants on the contrary, bring again immeasurable loss.
On the other hand, China has a large amount of stalks to be pulverized fermentation processing and be used as the feed of simple stomach (pig chicken and duck goose etc.) and many stomaches (cattle and sheep horse etc.) animal at present.The defect such as there is lignin due to stalk and crude fibre is many, protein content is low, quality is hard, palatability is poor, livestock is difficult to edible and digestion, people generally will enter microbial nutrition assistant agent and modulated by admixture in straw feed for this reason, to make feed, there is better palatability and consumption, help digesting and assimilating of letting animals feed, but also have effect instability for various straw fermented feed well known in the art is corresponding at present, difficult quality has controlled, production cost is high, expect the deficiencies such as consumption is large, fully can not meet the demand of modernization aquaculture.
Summary of the invention
An object of the present invention is the present situation for prior art, provides a kind of straw degradative agent utilizing stalk to make bacterium slag discarded after edible mushroom to produce for raw material, obtains efficient circulation utilization to make stalk resource; Another object of the present invention is the stalk organic feed providing a kind of improved stock keeping effect utilizing above-mentioned straw degradative agent to prepare.
One, straw degradative agent of the present invention is the product adopting following production stage obtained:
1, after the Herba Lycopi and kobold that wait weight portion are spent co-grinding, take water as solvent, being prepared into containing concentration of oligosaccharide is the solution of 10% ~ 15%, the fermented bacterium of solution weight 5% ~ 8% is added in solution, be prepared into microbe leaven, described fermented bacterium be following material by etc. the bacterial classification of weight ratio configuration: bacillus amyloliquefaciens (Bacillusamyloliquefaci..), bacillus coagulans (Bacillus coagulans), Bacillus acidi lactici (Lactobacillus), streptococcus (Streptococcus), saccharomyces cerevisiae (Sacchaomyces cerevisias), Weir yeast (Sacchaomyces willianus), bacillus cereus (Bacilluscereus), read coccus (Leuconostoc), bacillus (Tenacibaculum..) is sticked together addicted to middle temperature, streptomycete (Streptomycessp.), Pseudoalteromonas (Pseudoalteromon..), streptomyces antibioticus (Streptomycesant..), vibrios (Vibriosp.), clover sword bacterium (Ensifermedicae), lemon yellow red bacillus (Erythrobacterci..), rhizobium (Rhizobiumsp.), ooze sticks together bacillus (Tenacibaculumlu..), honeycomb series bacillus (Paenibacillus..), to dwell coral Pseudoalteromonas (Pseudoalt), balloon bacillus (Bacillusyesicuiferous), bread mold (Rhizopus nigrians), rhizopus chinensis (Rhizopuschinensis), above-mentioned each bacterial classification material is existing market material normally on sale,
2, the waste material bacterium ground-slag after the edible mushroom of production according to a conventional method utilizing stalk to be raw material (cultivating the edible mushrooms such as pleurotus eryngii, flat mushroom, auricularia auriculajudae, mushroom, straw mushroom, Ji mushroom, asparagus, Agricus blazei after namely utilizing crushed stalk to ferment) is broken into 60 ~ 70 object ground-slags, mix by the waste material of edible mushroom bacterium slag of 90 weight portions and the microbe leaven of 10 weight portions, adjustment moisture reaches the ratio of 60% ~ 65%, carries out conventional sterilant to the material after adjustment moisture;
3, will adjustment moisture fermentation vat put into by material after sterilizing, environment temperature be 20 ~ 28 DEG C without the condition of miscellaneous bacteria under carry out spontaneous fermentation in 3 ~ 5 days;
4, under lower than the condition of 60 DEG C, carry out drying to the material after spontaneous fermentation, removing moisture, makes the grain products of the straw degradative agent of 70 ~ 90 order granularities afterwards by material after drying by comminutor;
5, ventilative packaging is adopted to product, and according to product standard, product is detected, qualifiedly to dispatch from the factory.
The product standard of straw degradative agent of the present invention is that product every gram analyzes guarantee value at least 120 hundred million/more than g containing the effective CFU of viable bacteria, containing probiotic cell sum break through 22,000,000,000/gram more than, it analyzes guarantee value: 1,4 beta-glucanase > 50000u/g, cellulase > 2500u/g, zytase > 30000u/g, moisture < 12%.
Straw degradative agent of the present invention is that a kind of in use, have safe and reliable, simple, effect stability feature be composite solid microorganism formulation, multiple digestive ferment is rich in goods, the immune factor such as somatomedin, little peptide and bioactivator, its function is utilizable crude fibre by the mass degradation in all kinds of agricultural crop straw, utilize the effect of microbial enzyme, produce organic feed, fundamentally solve stalk comprehensive utilization problem, improve straw feed stock keeping effect.In practical application, straw feed is by after adding this straw degradative agent, can by most of agricultural crop straw, bagasse, pod shell class, manioc waste, vinasse, edible mushroom chaff slag, the raw material that the fiber contents such as rice bran are high is treated as the high-quality feed that simple stomach and many nonruminants all can be digested and assimilated in the short period of time, little peptide can be generated by fermented bean dregs with other protein raw materials, improve non-specific immunity, and complete feed and autogamy feed can be processed, and process wheat or secondary powder (improving flour and time powder consumption) etc., significantly improve letting animals feed palatability and digestibility, reduce material consumption.With the straw feed of this straw degradative agent modulation, can improve the immunity of animal, efficient-decomposition assimilation fecaluria, effectively reduces stink and the ammonia of plant, reduces excreta amount (environmental protection), reduce the incidence of disease, and amplitude peak ground reduces feeding cost.
Two, the stalk organic feed utilizing straw degradative agent to produce of the present invention comprises crude fibre stalk organic feed and waste material of edible mushroom bacterium slag stalk organic feed two kinds.
(1), crude fibre stalk organic feed adopts following production stage to obtain:
1, powder of straw is broken into 60 ~ 70 object ground-slags;
2, mixed by the straw degradative agent of 95 weight portions and 5 weight portions by the stalk after pulverizing, adjustment moisture reaches the ratio of 60% ~ 65%, then carries out conventional sterilant to the material after adjustment moisture;
3, will adjustment moisture fermentation vat put into by material after sterilizing, environment temperature be 25 ~ 35 DEG C without the condition of miscellaneous bacteria under carry out spontaneous fermentation in 5 ~ 7 days;
4, adopt the method for low temperature drying, make material under 60 DEG C ~ 80 DEG C conditions dry 6 ~ 12 hours, by comminutor, material after drying is made the grain products of 70 ~ 90 object crude fibre stalk organic feeds afterwards;
5, ventilative packaging is adopted to product, and according to product standard, product is detected, qualifiedly to dispatch from the factory.
(2), waste material of edible mushroom bacterium slag stalk organic feed adopts following production stage to obtain:
1, produce the waste material bacterium slag after edible mushroom be crushed into 60 ~ 70 object ground-slags by utilizing stalk to produce edible mushroom (namely utilize crushed stalk ferment then cultivate the edible mushrooms such as pleurotus eryngii, flat mushroom, auricularia auriculajudae, mushroom, straw mushroom, Ji mushroom, asparagus, Agricus blazei) according to a conventional method for raw material, mix by the waste material of edible mushroom bacterium slag of 95 weight portions and the straw degradative agent of 5 weight portions, adjustment moisture reaches the ratio of 60% ~ 65%, carries out conventional sterilant to the material after adjustment moisture;
2, will adjustment moisture fermentation vat put into by material after sterilizing, environment temperature be 25 ~ 35 DEG C without the condition of miscellaneous bacteria under carry out spontaneous fermentation in 3 ~ 5 days;
3, adopt the method for low temperature drying, make material under 60 DEG C ~ 80 DEG C conditions dry 6 ~ 12 hours, by comminutor, material after drying is made the grain products of the waste material of edible mushroom bacterium slag stalk organic feed of 70 ~ 90 order granularities afterwards;
4, ventilative packaging is adopted to product, and according to product standard, product is detected, qualifiedly to dispatch from the factory.
Compared with prior art, production process route of the present invention is simple, raw material sources is sufficient extensively, cost is low, there is novelty and the application and popularization value of production technology, fundamentally solve straw circulating problem of complex utilization, significantly can increase the income of peasant, reach the object of developing agricultural.
Detailed description of the invention
Embodiment one
The step of preparation process of a kind of straw degradative agent provided by the invention is as described below.
1, stalk process: by crushed stalk such as cornstalk, straw, beans bars, mix according to the part by weight of cornstalk 5, straw 3, beans bar 2;
2, produce pleurotus eryngii edible mushroom: added water to by the stalk be pulverized and mixed after 65%, adopt successively pack, sterilizing, inoculation, cultivation pleurotus eryngii edible mushroom conventional production process carry out pleurotus eryngii production;
3, utilize the bacterium slag after producing pleurotus eryngii for the agent of raw material production straw degradative:
A, the Chinese medicine Herba Lycopi genuine by tradition and kobold flower are by after the weight ratio co-grinding of 1: 1, take water as solvent, being prepared into containing concentration of oligosaccharide is the solution of 12%, the fermented bacterium of solution weight 6% is added in solution, be prepared into microbe leaven, described fermented bacterium be following material by etc. the bacterial classification (the weight allocation ratio between various bacterial classification is 1: 1) of weight ratio configuration: bacillus amyloliquefaciens, bacillus coagulans, Bacillus acidi lactici, streptococcus, saccharomyces cerevisiae, Weir yeast, bacillus cereus, read coccus, bacillus is sticked together addicted to middle temperature, streptomycete, Pseudoalteromonas, streptomyces antibioticus, vibrios, clover sword bacterium, lemon yellow red bacillus, rhizobium, ooze sticks together bacillus, honeycomb series bacillus, to dwell coral Pseudoalteromonas, balloon bacillus, bread mold, rhizopus chinensis,
B, ferment and cultivate the waste material bacterium ground-slag after pleurotus eryngii be broken into 60 object ground-slags by utilizing rice wheat straw to pulverize, mix by the waste material of edible mushroom bacterium slag of 90 weight portions and the microbe leaven of 10 weight portions, adjustment moisture reaches the ratio of 65%, then to the sterilizing that the material after adjustment moisture adopts steam to carry out under 1 atmospheric pressure, 115 DEG C of conditions 45 minutes;
C, will adjustment moisture fermentation vat put into by material after sterilizing, environment temperature be 25 DEG C without the condition of miscellaneous bacteria under carry out spontaneous fermentation in 5 days;
D, under lower than the condition of 60 DEG C, carry out drying to the material after spontaneous fermentation, removing moisture, makes the grain products of the straw degradative agent of 80 order granularities afterwards by material after drying by comminutor;
E, ventilative packaging is adopted to product, and according to product standard, product is detected, qualifiedly to dispatch from the factory.
Embodiment two
Utilize straw degradative agent of the present invention to can be used for producing multiple organic feed, the step of preparation process of a kind of crude fibre stalk organic feed is wherein as described below.
1, the powder of straw such as cornstalk, straw, beans bar are broken into 60 object ground-slags, according to cornstalk: straw: beans bar=5: the part by weight mixing of 3: 2;
2, by the embodiment one processing step obtained straw degradative agent of the mixed straw after pulverizing by 95 weight portions and 5 weight portions is mixed, adjustment moisture reaches the ratio of 60%, steam is adopted to carry out conventional sterilant (steam under an atmospheric pressure, 115 DEG C of conditions, sterilizing in 45 minutes) to the material after adjustment moisture;
3, will adjustment moisture fermentation vat put into by material after sterilizing, environment temperature be 30 DEG C without the condition of miscellaneous bacteria under carry out spontaneous fermentation in 7 days;
4, adopt the method for low temperature drying, make material under 70 DEG C of conditions dry 12 hours, by comminutor, material after drying is made the grain products of the crude fibre stalk organic feed of 80 order granularities afterwards;
5, ventilative packaging is adopted to product, and according to product standard, product is detected, qualifiedly to dispatch from the factory.
Embodiment three
The step of preparation process of a kind of asparagus waste material of edible mushroom bacterium slag stalk organic feed of the present invention is as described below.
1, stalk process: the powder of straw such as cornstalk, straw, beans bar are broken into 60 object ground-slags, according to cornstalk: straw: beans bar=5: the part by weight mixing of 3: 2;
2, produce asparagus edible mushroom: added water to by the stalk be pulverized and mixed after 65%, adopt successively pack, sterilizing, inoculation, cultivation asparagus edible mushroom conventional production process carry out apricot spore mushroom production;
3, utilize the bacterium slag after producing asparagus for the agent of raw material production straw degradative:
A, after the Herba Lycopi and kobold that wait weight portion are spent co-grinding, take water as solvent, being prepared into containing concentration is the solution of 15%, the fermented bacterium of solution weight 8% is added in solution, be prepared into microbe leaven, described fermented bacterium be following material by etc. the bacterial classification of weight ratio configuration: bacillus amyloliquefaciens, bacillus coagulans, Bacillus acidi lactici, streptococcus, saccharomyces cerevisiae, Weir yeast, bacillus cereus, read coccus, bacillus is sticked together addicted to middle temperature, streptomycete, Pseudoalteromonas, streptomyces antibioticus, vibrios, clover sword bacterium, lemon yellow red bacillus, rhizobium, ooze sticks together bacillus, honeycomb series bacillus, to dwell coral Pseudoalteromonas, balloon bacillus, bread mold, rhizopus chinensis,
B, ferment and cultivate the waste material bacterium ground-slag after asparagus be broken into 60 object ground-slags by utilizing rice wheat straw to pulverize, mix by the waste material of edible mushroom bacterium slag of 90 weight portions and the microbe leaven of 10 weight portions, adjustment moisture reaches the ratio of 65%, steam is adopted to carry out conventional sterilant (steam under 1 atmospheric pressure, 115 DEG C of conditions, sterilizing in 45 minutes) to the material after adjustment moisture;
C, will adjustment moisture fermentation vat put into by material after sterilizing, environment temperature be 25 DEG C without the condition of miscellaneous bacteria under carry out spontaneous fermentation in 5 days;
D, under lower than the condition of 60 DEG C, carry out drying to the material after spontaneous fermentation, removing moisture, makes the grain products of the straw degradative agent of 80 order granularities afterwards by material after drying by comminutor;
4, waste material of edible mushroom bacterium slag stalk organic feed is produced:
A, the waste material bacterium slag after producing asparagus edible mushroom is crushed into 60 object ground-slags, mix by the asparagus waste material of edible mushroom bacterium slag of 95 weight portions and the above-mentioned straw degradative agent of 5 weight portions, adjustment moisture reaches the ratio of 65%, then steam is adopted to carry out conventional sterilant (steam under 115 DEG C of conditions, sterilizing in 45 minutes) to the material after adjustment moisture;
B, will adjustment moisture fermentation vat put into by material after sterilizing, environment temperature be 30 DEG C without the condition of miscellaneous bacteria under carry out spontaneous fermentation in 5 days;
C, adopt the method for low temperature drying, make material under 75 DEG C of conditions dry 10 hours, by comminutor, material after drying is made the grain products of the asparagus waste material of edible mushroom bacterium slag stalk organic feed of 80 order granularities afterwards;
D, ventilative packaging is adopted to product, and according to product standard, product is detected, qualifiedly to dispatch from the factory.
Embodiment four
The application example of straw degradative agent of the present invention in aquaculture is as described below.
1, complete feed and autogamy feed is processed
(1), process contains the slightly high autogamy feed of crude fibre, straw degradative agent one described in the embodiment of the present invention one is wrapped (1 kilogram, lower same) be dissolved in (suggestion in winter warm water) in 250 kg of water, afterwards degradation agent solution is spread across in 400 kg diet and mixes thoroughly, beat heap to cover, process to feed after 2 hours ox, horse, this method is disposed and will feeds the same day, can lossy (if winter, then can deposit 2 days) more than 12 hours;
(2), autogamy feed or complete feed using method: the straw degradative agent one described in the embodiment of the present invention one is wrapped, is mixed into Direct-fed ox after in 600 kg diet, horse, ox, the horse fed of also can directly granulating;
(3), add in particle complete feed and feed: straw degradative agent one bag described in the embodiment of the present invention one is put into 3 ~ 5 kg of water (preferably the warm water of about 35 DEG C) more than 10 minutes, be sprayed onto in 40 kilograms of ox poultry feeds in 400 kg particles feeds, feed turned afterwards these feeds are fed a little; Also this straw degradative agent one can be wrapped and mix with 1 ~ 2 kilogram of corn flour, evenly be spread across in 600 kg particles feeds and mix up, make it stick to pellet and show to feed at once.
The additive effect of the present embodiment product: on the basis of original complete feed, then can significantly improve the speed of growth of feeding and raising animal, reduce feedstuff-meat ratio, meat flavor is obviously improved, delivers for sale significantly in advance, excrement stink significantly reduces.
The performance that the present embodiment product has: can reduce feed the enteron aisle PH raising animal, balance intestinal micro-ecological environment, reduce plant stink, Reverse transcriptase harmful bacteria, develop immunitypty, produces secondary antibiotic, improves the price of deed etc.
Claims (3)
1. a straw degradative agent, is characterized in that it is the product adopting following production stage obtained:
1.1 by after the Herba Lycopi of weight portion such as grade and kobold flower co-grinding, take water as solvent, being prepared into containing concentration of oligosaccharide is the solution of 10% ~ 15%, the fermented bacterium of solution weight 5% ~ 8% is added in solution, be prepared into microbe leaven, described fermented bacterium be following material by etc. the bacterial classification of weight ratio configuration: bacillus amyloliquefaciens, bacillus coagulans, Bacillus acidi lactici, streptococcus, saccharomyces cerevisiae, Weir yeast, bacillus cereus, read coccus, bacillus is sticked together addicted to middle temperature, streptomycete, Pseudoalteromonas, streptomyces antibioticus, vibrios, clover sword bacterium, lemon yellow red bacillus, rhizobium, ooze sticks together bacillus, honeycomb series bacillus, to dwell coral Pseudoalteromonas, balloon bacillus, bread mold, rhizopus chinensis,
1.2 are broken into 60 ~ 70 object ground-slags by utilizing stalk to produce the waste material bacterium ground-slag after edible mushroom for raw material, be uniformly mixed by the waste material of edible mushroom bacterium slag of 90 weight portions and the microbe leaven of 10 weight portions, adjustment moisture reaches the ratio of 60% ~ 65%, carries out conventional sterilant to the material after adjustment moisture;
1.3 will adjustment moisture fermentation vat put into by material after sterilizing, environment temperature be 20 ~ 28 DEG C without the condition of miscellaneous bacteria under carry out spontaneous fermentation in 3 ~ 5 days;
1.4 carry out drying to the material after spontaneous fermentation under lower than the condition of 60 DEG C, and removing moisture, makes the grain products of the straw degradative agent of 70 ~ 90 order granularities afterwards by material after drying by comminutor.
2. the crude fibre stalk organic feed utilizing straw degradative agent described in claim 1 to produce, is characterized in that it is the product adopting following production stage obtained:
Powder of straw is broken into 60 ~ 70 object ground-slags by 2.1;
Stalk after pulverizing mixes by the straw degradative agent of 95 weight portions and 5 weight portions by 2.2, and adjustment moisture reaches the ratio of 60% ~ 65%, carries out conventional sterilant to the material after adjustment moisture;
2.3 will adjustment moisture fermentation vat put into by material after sterilizing, environment temperature be 25 ~ 35 DEG C without the condition of miscellaneous bacteria under carry out spontaneous fermentation in 5 ~ 7 days;
2.4 adopt the method for low temperature dryings, make material under 60 DEG C ~ 80 DEG C conditions dry 6 ~ 12 hours, by comminutor, material after drying are made the grain products of 70 ~ 90 object crude fibre stalk organic feeds afterwards.
3. the waste material of edible mushroom bacterium slag stalk organic feed utilizing straw degradative agent described in claim 1 to produce, is characterized in that it is the product adopting following production stage obtained:
3.1 are crushed into 60 ~ 70 object ground-slags by utilizing stalk to produce the waste material bacterium slag after edible mushroom for raw material, mix by the waste material of edible mushroom bacterium slag of 95 weight portions and the straw degradative agent of 5 weight portions, adjustment moisture reaches the ratio of 60% ~ 65%, carries out conventional sterilant to the material after adjustment moisture;
3.2 will adjustment moisture fermentation vat put into by material after sterilizing, environment temperature be 25 ~ 35 DEG C without the condition of miscellaneous bacteria under carry out spontaneous fermentation in 3 ~ 5 days;
3.3 adopt the method for low temperature dryings, make material under 60 DEG C ~ 80 DEG C conditions, after dry 6 ~ 12 hours, by comminutor, material after drying to be made the grain products of the waste material of edible mushroom bacterium slag stalk organic feed of 70 ~ 90 order granularities.
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| CN106173239A (en) * | 2016-07-18 | 2016-12-07 | 柳州市柳南区安顺养殖协会 | A kind of processing method improving feedstuff lignocellulose degradation rate |
| CN107259080A (en) * | 2017-06-26 | 2017-10-20 | 舒丽 | A kind of food waste thing fodder processing method |
| CN109482618A (en) * | 2015-12-01 | 2019-03-19 | 北京德瑞丰农业科技有限责任公司 | The purposes of bacillus M2 degradation agricultural wastes |
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| CN115353993A (en) * | 2022-07-22 | 2022-11-18 | 黄淮学院 | Microbial straw degradation microbial inoculum and preparation method thereof |
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| CN115353993A (en) * | 2022-07-22 | 2022-11-18 | 黄淮学院 | Microbial straw degradation microbial inoculum and preparation method thereof |
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