WO2016200190A1 - 칠엽담으로부터 분리된 지페노사이드를 포함하는 주름 개선용 화장료 조성물 - Google Patents
칠엽담으로부터 분리된 지페노사이드를 포함하는 주름 개선용 화장료 조성물 Download PDFInfo
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- WO2016200190A1 WO2016200190A1 PCT/KR2016/006151 KR2016006151W WO2016200190A1 WO 2016200190 A1 WO2016200190 A1 WO 2016200190A1 KR 2016006151 W KR2016006151 W KR 2016006151W WO 2016200190 A1 WO2016200190 A1 WO 2016200190A1
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- zipenoside
- dammar
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
Definitions
- the present invention relates to a cosmetic composition for improving wrinkles comprising the chilbye zipinoside fraction as an active ingredient.
- Collagen makes up most of the extracellular matrix (ECM) produced by fibroblasts and secreted out of the cell, accounting for about 75% of dry skin weight and about 90% of dermal fibrous components. It maintains the elasticity of the skin while having important functions such as providing structural support and providing tensile strength of the skin. Synthesis and degradation of collagen in vivo is properly regulated, but as aging progresses, the synthesis decreases, and the expression of MMPs (matrix metalloproteinase), an enzyme that degrades collagen, increases, resulting in wrinkles.
- ECM extracellular matrix
- wrinkles There are various kinds of wrinkles. Depending on the characteristics of the wrinkles, it may be divided into static wrinkles and dynamic wrinkles, and depending on the position of the wrinkles, it may be divided into eye wrinkles and nasolabial folds. In addition, there are transparent wrinkles, which are wrinkles, which are not seen on the outside but proceed inside the skin.
- Ascorbic acid, alpha-tocopherol, retinol, and super oxide dismutase (SOD) have been used as substances to promote collagen production and improve skin wrinkles, but the physical and chemical stability is significantly lowered or the price is high. For this reason, the reality was that it was difficult to see the true effect on wrinkle improvement because it was not possible to use an effective amount that would have a substantial effect on skin wrinkle improvement.
- Noni fruit extract registered patent 10-0844275
- soft-leaved extract registered patent 10-0825450
- Plant-derived wrinkle improvement cosmetic raw materials such as green tea extract (registered patent 10-0757175) have been developed.
- conventional natural plant extracts have a problem that it is difficult to apply to cosmetics because the color is dark, strong fragrance and discoloration or discoloration occurs when the cosmetic composition is manufactured.
- Chilbyo Gynostemma pentaphyllum
- Dodol or Gyogoram a perennial vine plant belonging to the southern part of Korea, islands such as Jeju Island, Ulleungdo, and Cucurbitaceae that grows in China and Japan. It stretches and grows tangled with white hairs on the nodes, but also climbs up with tendrils. Tea made from dried leaves of chile leaves is mainly known as vine tea, which restores the function of organs, has anti-stress and antioxidant effects, and is known to be effective in asthma, bronchitis, and ulcers. Chil-yeopdam is also known for its skin-beauty effects, and women from China and North Korea have been known to drink tea to keep their skin healthy.
- the present inventors conducted a intensive study to solve the problems of the conventional natural plant materials, it was confirmed the excellent wrinkle improvement effect in Chilgapdam Zipenoside fraction which is not known conventionally wrinkle improvement. In particular, as well as general wrinkles, it was confirmed an excellent improvement effect on the invisible transparent wrinkles and completed the present invention.
- the problem to be solved of the present invention is to provide a cosmetic composition for improving wrinkles comprising chile chief phenoside fraction as an active ingredient.
- Another problem to be solved of the present invention is to provide a cosmetic composition for improving wrinkles comprising at least one phenenoside represented by the formula (I) as an active ingredient.
- Another object of the present invention is to provide a method for improving wrinkles using the cosmetic composition.
- the present invention to solve the above problems provides a cosmetic composition for improving wrinkles comprising the chilbye zipinoside fraction as an active ingredient.
- the chilbye zipenoside fraction is a zipenoside UL1, zipenoside UL2, zipenoside UL3, zipenoside UL4, zipenoside UL5, zipenoside UL6 and Zippe as defined below It provides a cosmetic composition for improving wrinkles comprising at least one zipenoside selected from the group consisting of Norside UL7:
- Zipenoside UL1 is a (23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23-epoxy-dammar-24-ene-3-O- [ ⁇ -L-ramnopyranosyl (1 ⁇ 2)]- ⁇ -L-arabinopyranoside (1-en-3-O- [ ⁇ arabinofyranoside ((23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23 -epoxy-dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1 ⁇ 2)]- ⁇ -L-arabinopyranoside);
- Zipenoside UL2 is (23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23-epoxy-dammar-24-ene-3-O- [ ⁇ -D-glucopyranosyl (1 ⁇ 3)]- ⁇ -L-arabinopyranoside (1cyen-3-O- [ ⁇ arabinopyranoside ((23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23- epoxy-dammar-24-ene-3-O- [ ⁇ -D-glucopyranosyl (1 ⁇ 3)]- ⁇ -L-arabinopyranoside);
- Zipenoside UL3 is (23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-21,23-epoxy-dammar-24-ene-3-O- [ ⁇ -L-ramnopyranosyl (1 ⁇ 2)] [ ⁇ -D-xylopyranosyl (1 ⁇ 3)]- ⁇ -L-arabinopyranoside ((23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-21,23-epoxy-dammar-24-ene- 3-O- [ ⁇ -L-rhamnopyranosyl (1 ⁇ 2)] [ ⁇ -D-xylopyranosyl (1opyranoLarabinopyranoside);
- Zipenoside UL4 is ((23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23-epoxy-dammar-24-ene-3-O- [ ⁇ -L-ramnopyranosyl ( 1 ⁇ 2)] [ ⁇ -D-xylopyranosyl (1 ⁇ 3)]- ⁇ -L-arabinopyranoside ((23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23 -epoxy- dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1mnopyranoxylopyranosyl (1opyranoLarabinopyranoside));
- Zipenoside UL5 is (23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23-epoxy-dammar-24-ene-3-O- [ ⁇ -L-lamnopyranosyl (1 ⁇ 2)] [ ⁇ -D-glucopyranosyl (1 ⁇ 3)]- ⁇ -L-arabinofyranoside ((23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23-epoxy dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1mnopyranoglucopyranosyl (1copyranLarabinopyranoside));
- Zipenoside UL6 is 3 ⁇ , 20S, 21-trihydroxy-19-oxo-dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1 ⁇ 2)] ⁇ [ ⁇ -D- Xylopyranosyl (1 ⁇ 6)] [ ⁇ -D-glucopyranosyl (1 ⁇ 3)] ⁇ - ⁇ -L-arabinofyranoside-21-O- ⁇ -D-glucopyranoside (3 ⁇ , 20S, 21-trihydroxy-19-oxo-dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1 ⁇ 2)] ⁇ [ ⁇ -D-xylopyranosyl (1 ⁇ 6)] [ ⁇ -D -glucopyranosyl (1 ⁇ 3)] ⁇ - ⁇ -L-arabinopyranoside-21-O- ⁇ -D-glucopyranoside); And
- Zipenoside UL7 is 3 ⁇ , 20S-dihydroxy-21-carboxyl-dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1 ⁇ 2)] ⁇ [ ⁇ -D-ch Silopyranosyl (1 ⁇ 6)] [ ⁇ -D-glucopyranosyl (1 ⁇ 3)] ⁇ - ⁇ -L-arabinofyranoside-21-O- ⁇ -D-glucopyranoside (3 ⁇ , 20S-dihydroxy-21-carboxyl-dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1 ⁇ 2)] ⁇ [ ⁇ -D-xylopyranosyl (1 ⁇ 6)] [ ⁇ -D-glucopyranosyl ( 1 ⁇ 3)] ⁇ - ⁇ -L-arabinopyranoside-21-O- ⁇ -D-glucopyranoside).
- zipenoside UL1, zipenoside UL2, zipenoside UL3, zipenoside UL4, zipenoside UL5, zipenoside UL6 and zipenoside UL7 is shown in FIG.
- the chilbye zipenoside fraction may be included in 0.001 to 30% by weight relative to the total weight of the composition. More specifically, the chilbye giphenoside fraction may be included in an amount of 0.01 wt% to 10 wt% based on the total weight of the composition.
- the wrinkles may be a cosmetic composition for improving wrinkles, characterized in that the transparent wrinkles.
- the transparent wrinkles are wrinkles found in the early 20s to early 30s, in which wrinkles are not observed around the eyes. Skin wrinkles are not observed with the naked eye, but the decrease in the dermal density inside the dermal layer is called transparent wrinkles. Transparent wrinkles can progress rapidly to the skin wrinkles appearing to the naked eye, management of the transparent wrinkles is becoming important.
- the chilbye zipinoside fraction may comprise at least one of zipenoside UL4 and zipenoside UL5 in 20 to 30% by weight relative to the weight of the total fraction.
- the phenenoside UL4 and the phenenoside UL5 in the chilbye zipinoside fraction is less than 20% by weight relative to the total weight of the fraction does not appear to improve the skin wrinkles, if more than 30% by weight Increasing the amount of skin wrinkle improvement effect to increase the content is insignificant, there is a problem in the stability of the formulation, it is not economical.
- the present invention to solve the above problems provides a cosmetic composition for improving wrinkles comprising at least one of the phenenosides represented by the formula (I) as an active ingredient:
- R 1 is a glycoside linked polysaccharide with the same or different sugar selected from the group consisting of rhamnose, xylose, glucose and arabinose,
- R 2 is methyl (CH 3 ) or aldehyde (CHO),
- R 3 is , , , , , or to be.
- the polysaccharide refers to saccharides in which two or more monosaccharides form a molecule through glycosidic bonds.
- the molecular weight varies from thousands to millions, and storage polysaccharides such as starch or glycogen, and structural properties such as cellulose and chitin.
- Polysaccharides and the like can be included here, but are not limited thereto.
- the monosaccharide has the simplest structure that is no longer hydrolyzed by acid, base, enzyme, etc., such as rhamnose, fucose, arabinose, xylose, mannose, galactose and glucose.
- Carbohydrate basic monomers and the like may be included here, but is not limited thereto.
- the glycosidic linkage refers to a chemical bond in which a link between carbon of one sugar molecule and another sugar molecule is linked by an oxygen atom.
- Sugars containing aldehydes or ketones form intramolecular cyclic hemiacetals or hemiketal structures. The hydroxy groups newly formed by the formation of these structures are called ananomeric hydroxyl groups, and the bonds formed by substituting these anomeric hydroxy groups are glycosidic bonds. to be.
- Glycoside bonds derived from aldehydes are called aldoside bonds
- glycoside bonds derived from ketones are called ketoside bonds.
- the zipinoside may be extracted or separated from the chilebdam, but is not limited thereto.
- the zipenoside is a zipenoside UL1, zipenoside UL2, zipenoside UL3, zipenoside UL4, zipenoside UL5, zipenoside UL6 and zipenoside UL7 defined below It provides a cosmetic composition for improving wrinkles, characterized in that at least one selected from the group consisting of:
- Zipenoside UL1 is a (23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23-epoxy-dammar-24-ene-3-O- [ ⁇ -L-ramnopyranosyl (1 ⁇ 2)]- ⁇ -L-arabinopyranoside (1-en-3-O- [ ⁇ arabinofyranoside ((23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23 -epoxy-dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1 ⁇ 2)]- ⁇ -L-arabinopyranoside);
- Zipenoside UL2 is (23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23-epoxy-dammar-24-ene-3-O- [ ⁇ -D-glucopyranosyl (1 ⁇ 3)]- ⁇ -L-arabinopyranoside (1cyen-3-O- [ ⁇ arabinopyranoside ((23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23- epoxy-dammar-24-ene-3-O- [ ⁇ -D-glucopyranosyl (1 ⁇ 3)]- ⁇ -L-arabinopyranoside);
- Zipenoside UL3 is (23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-21,23-epoxy-dammar-24-ene-3-O- [ ⁇ -L-ramnopyranosyl (1 ⁇ 2)] [ ⁇ -D-xylopyranosyl (1 ⁇ 3)]- ⁇ -L-arabinopyranoside ((23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-21,23-epoxy-dammar-24-ene- 3-O- [ ⁇ -L-rhamnopyranosyl (1 ⁇ 2)] [ ⁇ -D-xylopyranosyl (1opyranoLarabinopyranoside);
- Zipenoside UL4 is ((23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23-epoxy-dammar-24-ene-3-O- [ ⁇ -L-ramnopyranosyl ( 1 ⁇ 2)] [ ⁇ -D-xylopyranosyl (1 ⁇ 3)]- ⁇ -L-arabinopyranoside ((23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23 -epoxy- dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1mnopyranoxylopyranosyl (1opyranoLarabinopyranoside));
- Zipenoside UL5 is (23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23-epoxy-dammar-24-ene-3-O- [ ⁇ -L-lamnopyranosyl (1 ⁇ 2)] [ ⁇ -D-glucopyranosyl (1 ⁇ 3)]- ⁇ -L-arabinofyranoside ((23 ⁇ ) -3 ⁇ , 20 ⁇ , 21 ⁇ -trihydroxy-19-oxo-21,23-epoxy dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1mnopyranoglucopyranosyl (1copyranLarabinopyranoside));
- Zipenoside UL6 is 3 ⁇ , 20S, 21-trihydroxy-19-oxo-dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1 ⁇ 2)] ⁇ [ ⁇ -D- Xylopyranosyl (1 ⁇ 6)] [ ⁇ -D-glucopyranosyl (1 ⁇ 3)] ⁇ - ⁇ -L-arabinofyranoside-21-O- ⁇ -D-glucopyranoside (3 ⁇ , 20S, 21-trihydroxy-19-oxo-dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1 ⁇ 2)] ⁇ [ ⁇ -D-xylopyranosyl (1 ⁇ 6)] [ ⁇ -D -glucopyranosyl (1 ⁇ 3)] ⁇ - ⁇ -L-arabinopyranoside-21-O- ⁇ -D-glucopyranoside); And
- Zipenoside UL7 is 3 ⁇ , 20S-dihydroxy-21-carboxyl-dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1 ⁇ 2)] ⁇ [ ⁇ -D-ch Silopyranosyl (1 ⁇ 6)] [ ⁇ -D-glucopyranosyl (1 ⁇ 3)] ⁇ - ⁇ -L-arabinofyranoside-21-O- ⁇ -D-glucopyranoside (3 ⁇ , 20S-dihydroxy-21-carboxyl-dammar-24-ene-3-O- [ ⁇ -L-rhamnopyranosyl (1 ⁇ 2)] ⁇ [ ⁇ -D-xylopyranosyl (1 ⁇ 6)] [ ⁇ -D-glucopyranosyl ( 1 ⁇ 3)] ⁇ - ⁇ -L-arabinopyranoside-21-O- ⁇ -D-glucopyranoside).
- the composition may comprise 0.0001 to 30% by weight of the zipenoside relative to the total composition weight.
- the phenenoside is less than 0.0001% by weight of the total composition weight does not appear to improve the skin wrinkles, when it exceeds 30% by weight of the increase in the skin wrinkle improvement effect for the increase of the content is insignificant, There is a problem, not economical.
- the composition may include at least one of zipenoside UL4 and zipenoside UL5 in 0.0002 to 9% by weight relative to the total weight of the composition. If the zipinoside is less than 0.0002% by weight relative to the total composition weight of at least one of UL4 and zipenoside UL5 does not appear to improve the skin wrinkles, if more than 9% by weight increase the skin wrinkle improvement effect for the content increase The degree is minor, there is a problem with formulation stability, and it is not economical.
- the present invention provides a method for improving wrinkles by using the cosmetic composition.
- the phenenosides may be differently referred to as dammarane type saponins, triterpene saponins, gynosaponins, and other names of phenenosides commonly used in the art. It is included in the scope of the invention.
- step (i) the mixed solvent of purified water and ethanol, which is 5-20 times the mass-to-volume ratio of the dried / cut chile wall surface (leaves and stems), is added, stirred and extracted at 50-80 ° C., and then filtered. It is possible to obtain the chile chile extract crude.
- the crude ethanol extract obtained above is adsorbed on a column filled with a porous polymer synthetic adsorption resin, washed with purified water and a mixed solvent of ethanol, and eluted to obtain an eluate obtained by drying. can do.
- the extraction solvent in step (i) and the washing and elution solvent in step (ii) include one or two of purified water, alcohol, C 1 to C 4 lower alcohols, ethyl acetate, butylene glycol, and propylene glycol. The above can be used.
- porous polymer synthetic adsorption resin in step (ii) single or two or more selected from the group consisting of Diaion HP20, HP21 or HP2MG resin may be used.
- Chilgapdam Zipenoside fraction eluted through the above column was concentrated under reduced pressure to evaporate the solvent and then lyophilized to obtain a powder or an aqueous solution in which the content of solids was kept constant. Can be used.
- the method for preparing zipenoside according to the present invention is not limited thereto.
- the zipenoside may be extracted from plants other than Chilbale and may also be synthesized by a general chemical synthesis method.
- Raw material of chile yeopdam to obtain the chileokdam giphenoside fraction can be used to be grown in Ulleung-gun, Gyeongsangbuk-do, but is not limited thereto.
- the cosmetic composition of the present invention may include components commonly used in cosmetic compositions, for example surfactants, pH adjusters, pigments, flavorings, pigments, preservatives, fungicides, thickeners, antioxidants, stabilizers, dissolution
- various additives such as topical agents, metal ion sequestrants, cooling agents, deodorants, emulsifiers, solubilizers, moisturizers, ultraviolet absorbers, ultraviolet scatterers, vitamins, plant extracts, skin astringents, anti-inflammatory agents, cell activators, vasodilators, blood circulation
- Conventional adjuvants such as accelerators, and anti-skin functioning agents, and carriers, and the like, and the formulations and additives thereof are not limited to the above.
- the ingredients may also be introduced in amounts generally used in the field of dermatology.
- surfactant examples include, but are not limited to, anions such as higher fatty acid soaps, alkyl sulfate ester salts, polyoxyethylene alkyl ether sulfates, alkyl ether phosphate ester salts, N-acylamino acid salts, acyl N-methyltaurine salts, and the like.
- Surfactants Cationic surfactants such as alkyltrimethyl ammonium chloride and dialkyldimethyl ammonium chloride; Amphoteric surfactants such as alkyl dimethylaminoacetic acid betaine, alkyl amidodimethylaminoacetic acid betaine and 2-alkyl-N-carboxy-N-hydroxyimidazolinium betaine; And nonionic surfactants such as polyoxyethylene type, polyhydric alcohol ester type and ethylene oxide / propylene oxide block copolymers. Moreover, the surfactant which belongs to a polymeric surfactant or a natural surfactant can also be used without a restriction
- the thickener for example, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxyvinyl polymer, alkyl modified carboxyvinyl polymer, polyacrylamide Sodium Arginate, Sodium Alginate, Propylene Glycol Alginate, Agar, Sodium Polyacrylate, Succinoglucan, Dextran, Mannan, Pectin ), Gelan Gum, Carrageenan, Hyaluronic acid, Polyvinyl alcohol, Bentonite, Aluminum Silicate, Magnesium Silicate, Laponite (Laponite), hectorite (hectorite), silicic acid anhydride (Silicic acid Anhydride) and the like.
- paraoxy benzoate ethyl, salicylic acid, sorbic acid, etc. can be illustrated, for example.
- moisturizing agent examples include hyaluronic acid, chondroitin sulfate, pyrrolidone carboxylate, polyoxyethylene methyl glucoside, polyoxypropylene methyl glucoside, lecithin, phosphatidylcholine, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, sphingophospholipid, and the like. Can be illustrated.
- metal ion sequestrant examples include edetic acid salts such as ethylenediamine disodium tetraacetate, edetic acid and sodium edate salt.
- the emulsifier examples include hydrocarbons such as squalene, liquid paraffin, petrolatum and solid paraffin; oils such as olive oil, almond oil, cacao fat and castor oil; lead such as beeswax, lanolin and jojoba oil; stearic acid, oleic acid, palmitic acid and the like. Higher alcohols such as fatty acids, cetanol, stearyl alcohol, esters such as IPM, glycerintriester, pentaerythritol tetra ester, silicone oils such as polysiloxanes, and the like.
- hydrocarbons such as squalene, liquid paraffin, petrolatum and solid paraffin
- oils such as olive oil, almond oil, cacao fat and castor oil
- lead such as beeswax, lanolin and jojoba oil
- stearic acid oleic acid, palmitic acid and the like.
- Higher alcohols such as fatty acids, cetanol, ste
- solubilizer examples include propylene glycol fatty acid esters such as glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, and propylene glycol monostearate, glycerin, diglycerine, ethylene glycol, propylene glycol, 1,3-butyl Polyhydric alcohols, such as len glycol, etc. are mentioned.
- flavors include, but are not limited to, vegetable flavors isolated from flowers, leaves, branches, rinds, etc .; Animal flavors such as musk and civit; Hydrocarbons such as monoterpene; Alcohols such as aliphatic alcohols and aromatic alcohols; Aldehydes such as terpenaldehyde and aromatic aldehydes; Ketones such as alicyclic ketones; Esters such as terpene esters; Lactones; Phenols; Oxides; Nitrogen-containing compounds; Acetals etc. are mentioned.
- natural or synthetic flavors other than those exemplified above may be used without particular limitation.
- the cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and in one embodiment may be any one formulation selected from the group consisting of a high viscosity emulsifier type, a low viscosity emulsifier type, and a solubilized formulation,
- the present invention is not limited thereto, and may include a cosmetic composition blending component commonly used in the art according to the formulation to be prepared. Examples include solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, packs, massage creams and sprays, etc. It may be, but is not limited thereto.
- flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, sun lotion, sun cream, makeup base, BB cream, cleansing cream, cleansing foam, cleansing water, pack, stick form product, balm It can be prepared in the form of Balm type products, sprays or powders.
- a water phase component an oil phase component, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a preservative, a fragrance and the like.
- the carrier component is animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or Zinc oxide or the like can be used.
- a solvent, a solubilizing agent or an emulsifying agent is used as the carrier component.
- a solvent, a solubilizing agent or an emulsifying agent is used as the carrier component.
- water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzo Fatty acid esters of ate, propylene glycol, 1,3-butylene glycol oil, glycerol aliphatic esters, polyethylene glycols or sorbitan may be used.
- a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester are used as carrier components.
- Suspending agents, microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tragacanth and the like can be used.
- lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component when the formulation is a powder or a spray, and in particular, in the case of a spray, additionally chlorofluoro Propellants such as rohydrocarbon, propane / butane or dimethyl ether.
- the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a sarcosinate, a fatty acid amide.
- Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.
- the cosmetic composition of the present invention in the case of a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation or soap, it may be wiped off, peeled off or washed with water after application to the skin.
- the soap is a liquid soap, powdered soap, solid soap and oil soap
- the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towels and cleansing pack
- the surfactant-free cleansing formulation is a cleansing cream, Cleansing lotion, cleansing water and cleansing gel.
- the cosmetic composition according to the present invention including chilbye zipinoside fraction, has an excellent wrinkle improvement, skin elasticity enhancement and skin dermal density improvement effect.
- chilbye Zipenoside fraction of the present invention by promoting the production of procollagen exhibited excellent wrinkle improvement, skin elasticity enhancement and skin dermal density improvement effect.
- the chilbye zipinoside fraction is safe without irritation and irritation to the skin and excellent stability of the active ingredient under various storage conditions.
- the chilbye zipinoside fraction is not discolored even after long-term storage under high temperature or sunlight, the content of the active ingredient is kept constant.
- the cosmetic composition comprising the chilbye giphenoside fraction of the present invention as an active ingredient has an excellent wrinkle improvement effect.
- Figure 1 shows the structural formula of zipinoside UL1 to UL7.
- Figure 2 is a chromatogram analyzed by HPLC-UVD fraction of chile chiffon Zipenoside fraction.
- Figure 3 shows the experimental results of the procollagen production effect of the chief yeast giphenoside fraction and the fenenosides UL4 and UL5, and the positive control N-acetyl glucosamine (NAG).
- Figure 4 shows the cytotoxicity test results to determine the safety of chile chiffon Zipenoside fractions.
- Figure 5 is a clinical trial result graph showing the eye wrinkle inhibitory effect of chile chiffon Zipenoside fraction.
- Figure 6 is a graph showing the change in the wrinkles of the eye according to whether or not the chilbye zipinoside fraction application.
- Figure 7 is a graph of the clinical trial results showing the skin elasticity improvement effect of chile chiffon Zipenoside fraction.
- Figure 8 is a graph showing the change in skin elasticity according to whether the application of chile chiffon Zipenoside fraction.
- FIG. 9 is a graph showing the results of clinical trials showing the dermal dermal density improvement effect of chile chiffon Zipenoside fraction.
- Figure 10 is a graph showing the skin dermal density change according to whether the application of chile chiffon Zipenoside fraction.
- Example 1 After filling the column with a porous synthetic adsorbent HP-20, 40% ethanol was added to equilibrate, and then the crude yeast ethanol crude extract obtained in Example 1-1) was diluted in 40% ethanol and slowly loaded. Subsequently, 45% ethanol is sufficiently added to remove impurities, and then eluted with 80% ethanol to obtain the chilbale zipinoside fraction. The eluate was concentrated under reduced pressure, ethanol was evaporated, and lyophilized to obtain about 30 g of chile chief phenoside fraction in powder form.
- Example 2 Separation of the active ingredient from the chilewood fenofoxide fraction obtained in Example 1 was carried out as follows. 21 g of chile chief phenoside fraction obtained in Example 1 was taken and placed in a column (4.6 cm in diameter x 31 cm in height) using 323 g of silica gel. v / v) 2 L, 5: 1 mixed solvent (v / v) 3 L, 3: 1 mixed solvent (v / v) 2 L, 1: 1 mixed solvent (v / v) Chromatography was performed. Fr. obtained through the four mixed solvents. A (1.56 g) (8: 1 mixed solvent), Fr. B (5.99 g) (5: 1 mixed solvent), Fr. C (3.12 g) (3: 1 mixed solvent), Fr.
- zipenoside UL1, zipenoside UL2, zipenoside UL3, zipenoside UL4, zipenoside UL5, zipenoside UL6 and zipenoside UL7 is shown in FIG.
- Example 2 Chilbale Zipopenoside fractions obtained through Example 1 were analyzed by HPLC. Zipenoside UL1 to Zipenoside UL7 identified through Example 2 are shown in FIG. 2 by HPLC chromatogram.
- the zipenoside UL4 and zipenoside UL5 are separated with high content and high purity unlike the zipenoside UL1, zipenoside UL2, zipenoside UL3, zipenoside UL6 and zipenoside UL7. Confirmed that it can.
- Example 1 was analyzed in HPLC-UVD 203 nm of Cip columnar Zipenoside fraction obtained in Example 1 using a C18 column of diameter 4.6mm, length 25cm under the concentration gradient of acetonitrile, distilled water, obtained through Example 1 Chilgyedam Zipenoside fraction was analyzed to contain 121mg of Zipenoside UL4 and 95.6mg of Zipenoside UL5 per gram of powder.
- Human dermal fibroblast neonatal (Cascade Co.) was inoculated into 24-well microplates using DMEM medium supplemented with 10% fetal calf serum (5 ⁇ 10 4 cells / well), 37 ° C. Incubated for 24 hours at 5% CO 2 . After incubation for 24 hours by replacing with DMEM medium containing no serum, the appropriate amount of the sample solution was treated so that the final concentration of each test compound was 1 ⁇ g / ml and 5 ⁇ g / ml. After 48 hours of incubation, the cell culture fluid was collected, and then the amount of procollagen was measured using a collagen measurement kit by Takara Shuzo Co., Ltd.
- the cell culture fluids were collected into 96-well microplates uniformly coated with primary collagen antibodies, and antigen-antibody reactions occurred at 37 ° C. for 3 hours.
- Cell cultures in the wells were removed and washed four times with PBS.
- 1 N sulfuric acid solution was added to stop the reaction.
- Absorbance at wavelength 450nm was measured with a spectrophotometer.
- a standard curve was prepared using the standard solution, and the absorbance obtained by the above method was substituted into the standard curve to calculate the amount of procollagen production of the cell culture solution to which each test compound was added.
- NAG N-acetyl glucosamine
- the procollagen production effect of the yeast chile phenoside fraction and the fenofide UL4 and UL5 separated therefrom was compared with the procollagen production effect of NAG, a positive control, at the same concentration or at the same concentration. It was found to be equivalent or superior as producing large amounts of procollagen.
- Human dermal fibroblast neonatal (Cascade Co.) was inoculated into 24-well microplates using Dulbeco's Modified Eagle's Medium (DMEM) medium supplemented with 10% fetal bovine serum (5 ⁇ 10 4 cells). / Well), 37 °C, incubated for 24 hours at 5% CO 2 conditions. After incubation for 24 hours after replacing with serum-free DMEM medium was treated with chilephage zipinoside fraction obtained in Example 1 and the zipinoside UL4 and UL5 isolated from it. After 48 hours of incubation, MTT solution was added and incubated for 4 hours. The medium was removed and DMSO added to dissolve MTT formazan. MTT formazan dissolved in DMSO was taken and measured for absorbance at 570 nm, which was expressed as% control compared to the group not treated with the sample.
- DMEM Dulbeco's Modified Eagle's Medium
- fetal bovine serum 5 ⁇ 10 4 cells
- Well 37 °C
- the cosmetic composition of the various formulations containing the chilewood fenofoxide fraction obtained in Example 1 according to the composition described in Tables 4 to 6 was prepared.
- the following formulation examples are for illustrating the present invention more specifically, and the present invention is not limited only to the following examples.
- Formulations of high viscosity emulsion formulations containing chile chief Zipenoside fractions are shown in Table 4.
- Addition Phase I is a phase in which chilephage Zipenoside fractions are dissolved at 50 ° C. and mixed, and then Addition Phase II is mixed.
- Addition Phase I is a phase in which chilephage Zipenoside fractions are dissolved at 50 ° C. and mixed, and then Addition Phase II is mixed.
- Soluble image was added to the aqueous phase and mixed to obtain an oil-in-water emulsion.
- the cosmetic compositions of Formulation Examples 1, 2, and 3 were stored at 4 ° C., 30 ° C., 45 ° C., for 12 weeks under conditions of daylight, and observed changes in color, odor, and appearance of the formulation through visual and sensory evaluation. 7 to 9 are shown.
- the chilbye zipenoside fraction does not show discoloration, discoloration and change in appearance even after long-term storage at high temperature and sunlight conditions in the cosmetic composition of high viscosity emulsion formulation, low viscosity emulsion formulation, and solubilized formulation. Excellent stability was confirmed.
- Cosmetic compositions of high viscosity emulsion formulations, low viscosity emulsion formulations, and solubilized formulations containing 0.2% of chilbap zipenoside fractions as in Formulation Examples 1, 2 and 3 were subjected to 4 weeks, 30 degrees Celsius, 45 degrees Celsius, and 12 weeks of sunlight.
- Chilgyodam zipenoside fraction is a high viscosity emulsion formulation, low viscosity emulsion formulation, solubilization
- the cosmetic composition of the formulation it was confirmed that excellent stability does not decrease even after long-term storage at high temperature and sunlight conditions.
- the face was divided into two halves and the right and left sides were measured 2 cm away from the tail.
- R2 (Maximum roughness) of wrinkle degree parameters was measured using Visiometer SV600 (Courage-Khazaka electronic GmbH, Germany), and the results of the wrinkle improvement effect are shown in FIGS. 5 and 6.
- the area using the control product did not show any improvement in the eye wrinkles compared to before use, but the eye wrinkles in the area using the test product were improved from 69.10 to 43.97. (P ⁇ 0.05).
- Skin elasticity was measured using Cutometer® (Courage + Khazaka electronic GmbH, Germany), and the measurement was performed once at the same site of both eyes, and R2 (skin remodeling force) value was used as an evaluation data of skin elasticity.
- the R2 value is the overall elasticity, and the closer to 1, the more elastic the skin is.
- the skin elasticity measured by the use of the cream containing the chile chiffon Zipenoside fraction is shown in Figures 7 and 8.
- the skin elasticity of the test product used area increased to 0.66 after 12 weeks of use compared to 0.58 before use, and it was confirmed that the skin elasticity increased significantly (p ⁇ 0.05) compared to the skin elasticity of the control product used area. ..
- Dermal dermal density was measured by skin scanner high resolution ultrasound (taberna pro medicum, Germany) and the skin dermal density was analyzed before and after using the product using the measured images.
- the unit of measurement of skin dermal density is%, indicating that the higher the measured value, the better the dermal density.
- 9 and 10 show the results of measuring the skin dermal density improvement effect by using a cream containing chile chiffon zipinoside fraction.
- the skin dermal density of the site using the control product did not improve at a significant level after 12 weeks of use, but the skin dermal density of the site using the test product was 12 weeks after use (20.78%) compared to before use (16.53%). A significant improvement was found.
- Transparent wrinkles means that signs of aging of the skin are observed as the age increases before wrinkles are formed around the eyes. Transparent wrinkles can be identified through the dermal density.
- Severity Wrinkle grading 0 None One none / mild (no or weak wrinkles) 2 Mild (weak wrinkle) 3 mild / moderate 4 Moderate (medium wrinkle) 5 moderate / severe (medium or severe wrinkles) 6 Severe (severe wrinkles) 7 very severe
- cream was prepared containing 0.4% chilebdam Zipenoside fractions, the preparation composition is shown in Table 15 below.
- the skin dermis density tended to increase overall after using the cream containing chile chiffon Zipenoside fraction. It was found that the wrinkles, which are found but not visible to the naked eye, are improved overall.
Abstract
Description
시료 | 처리 농도 (ug/ml) | HDFn Viability (%) |
칠엽담 지페노사이드 분획물 | 1 | 98 |
5 | 99 | |
10 | 100 | |
20 | 112 | |
지페노사이드 UL4 | 5 | 98 |
10 | 100 | |
50 | 106 | |
지페노사이드 UL5 | 5 | 100 |
10 | 102 | |
50 | 113 |
번호 | 나이 | 성별 | 0.2% | 0.4% | 1.0% | ||||||
1일 | 2일 | 6일 | 1일 | 2일 | 6일 | 1일 | 2일 | 6일 | |||
1 | 31 | 여 | - | - | - | - | - | - | - | - | - |
2 | 49 | 여 | - | - | - | - | - | - | - | - | - |
3 | 49 | 여 | - | - | - | - | - | - | - | - | - |
4 | 55 | 여 | - | - | - | - | - | - | - | - | - |
5 | 43 | 여 | - | - | - | - | - | - | - | - | - |
6 | 33 | 여 | - | - | - | - | - | - | - | - | - |
7 | 55 | 여 | - | - | - | - | - | - | - | - | - |
8 | 43 | 여 | - | - | - | - | - | - | - | - | - |
9 | 43 | 여 | - | - | - | - | - | - | - | - | - |
10 | 50 | 여 | - | - | - | - | - | - | - | - | - |
11 | 24 | 여 | - | - | - | - | - | - | - | - | - |
12 | 54 | 여 | - | - | - | - | - | - | - | - | - |
13 | 42 | 여 | - | - | - | - | - | - | - | - | - |
14 | 42 | 여 | - | - | - | - | - | - | - | - | - |
15 | 45 | 여 | - | - | - | - | - | - | - | - | - |
16 | 53 | 여 | - | - | - | - | - | - | - | - | - |
17 | 48 | 여 | - | - | - | - | - | - | - | - | - |
18 | 51 | 여 | - | - | - | - | - | - | - | - | - |
19 | 51 | 여 | - | - | - | - | - | - | - | - | - |
20 | 26 | 여 | - | - | - | - | - | - | - | - | - |
21 | 46 | 여 | - | - | - | - | - | - | - | - | - |
22 | 55 | 여 | - | - | - | - | - | - | - | - | - |
23 | 51 | 여 | - | - | - | - | - | - | - | - | - |
24 | 54 | 여 | - | - | - | - | - | - | - | - | - |
25 | 35 | 여 | - | - | - | - | - | - | - | - | - |
26 | 39 | 여 | - | - | - | - | - | - | - | - | - |
27 | 36 | 여 | - | - | - | - | - | - | - | - | - |
28 | 34 | 여 | - | - | - | - | - | - | - | - | - |
29 | 28 | 여 | - | - | - | - | - | - | - | - | - |
30 | 39 | 여 | - | - | - | - | - | - | - | - | - |
Mean score | 0.00 | 0.00 | 0.00 | ||||||||
판정 | 자극 무 | 자극 무 | 자극 무 |
경과시간 | 칠엽담 지페노사이드 분획물의 원료 용액 (%) | |||||||
지페노사이드 UL4 | 지페노사이드 UL5 | |||||||
4℃ | 30℃ | 45℃ | 일광 | 4℃ | 30℃ | 45℃ | 일광 | |
1주 | 100.2 | 102.8 | 97.1 | 97.6 | 98.8 | 101.8 | 97.6 | 101.1 |
2주 | 98.1 | 99.9 | 96.3 | 96.6 | 101.3 | 98.2 | 97.5 | 98.4 |
4주 | 96.9 | 98.7 | 102.7 | 96.6 | 97 | 99 | 96.9 | 98.8 |
8주 | 98.3 | 96.5 | 97.5 | 98.1 | 97.9 | 101.2 | 99.2 | 97.2 |
12주 | 96.1 | 96.6 | 102.4 | 97.1 | 98.1 | 102.9 | 103.8 | 97.8 |
결과 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 |
상 | 성분 | 함량 ( % ) |
수상 | 정제수 | To 100 |
보습성분 | 10 ~ 25 | |
점증제 | 적량 | |
금속이온 봉쇄제 | 적량 | |
유상 | 피이지-100스테아레이트 | 0.1 ~ 2 |
글리세릴스테아레이트 | 0.1 ~ 2 | |
폴리소르베이트 60 | 0.1 ~ 2 | |
스테아릭애씨드 | 0.1 ~ 2 | |
세테아릴알코올 | 0.1 ~ 2 | |
카프릭카프릴릭트리글리세라이드 | 10 ~ 30 | |
토코페릴아세테이트 | 0.1 ~ 0.5 | |
첨가 I | 칠엽담 지페노사이드 분획물 | 0.20 |
첨가 II | 향 | 적량 |
보존제 | 적량 | |
기타 첨가제 | 적량 |
상 | 성분 | 함량 ( % ) |
수상 | 정제수 | To 100 |
세테아리스-6올리베이트 | 0.1 ~ 3 | |
보습성분 | 10 ~ 25 | |
점증제 | 적량 | |
금속이온 봉쇄제 | 적량 | |
유상 | 피이지-100스테아레이트 | 0.1 ~ 1 |
글리세릴스테아레이트 | 0.1 ~ 1 | |
폴리소르베이트 60 | 0.1 ~ 1 | |
세틸알코올 | 0.1 ~ 1 | |
베헤닐알코올 | 0.1 ~ 1 | |
스쿠알란 | 5 ~ 20 | |
토코페릴아세테이트 | 0.1 ~ 0.5 | |
첨가상 I | 칠엽담 지페노사이드 분획물 | 0.20 |
첨가상 II | 향 | 적량 |
보존제 | 적량 | |
기타 첨가제 | 적량 |
상 | 성분 | 함량 ( % ) |
수상 | 정제수 | To 100 |
보습성분 | 10 ~ 25 | |
점증제 | 적량 | |
금속이온 봉쇄제 | 적량 | |
가용화상 | 피이지-60하이드로제네이티드캐스터오일 | 0 ~ 2 |
피이지-40하이드로제네이티드캐스터오일 | 0 ~ 2 | |
다가알코올 | 0 ~ 10 | |
향료 | 적량 | |
에탄올 | 0 ~ 20 | |
첨가 I | 칠엽담 지페노사이드 분획물 | 0.20 |
첨가 II | 보존제 | 적량 |
기타 첨가제 | 적량 |
경과시간 | 고점도 유화 제형 | |||||||||||
취 변화 | 색 변화 | 제형 변화 | ||||||||||
4℃ | 30℃ | 45℃ | 일광 | 4℃ | 30℃ | 45℃ | 일광 | 4℃ | 30℃ | 45℃ | 일광 | |
1주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
2주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
4주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
8주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
12주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
결과 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 |
경과시간 | 저점도 유화 제형 | |||||||||||
취 변화 | 색 변화 | 제형 변화 | ||||||||||
4℃ | 30℃ | 45℃ | 일광 | 4℃ | 30℃ | 45℃ | 일광 | 4℃ | 30℃ | 45℃ | 일광 | |
1주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
2주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
4주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
8주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
12주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
결과 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 결과 | 안정 | 안정 | 안정 |
경과시간 | 가용화 제형 | |||||||||||
취 변화 | 색 변화 | 제형 변화 | ||||||||||
4℃ | 30℃ | 45℃ | 일광 | 4℃ | 30℃ | 45℃ | 일광 | 4℃ | 30℃ | 45℃ | 일광 | |
1주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
2주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
4주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
8주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
12주 | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ | ○ |
결과 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 |
경과시간 | 고점도 유화 제형 (%) | |||||||
지페노사이드 UL4 | 지페노사이드 UL5 | |||||||
4℃ | 30℃ | 45℃ | 일광 | 4℃ | 30℃ | 45℃ | 일광 | |
1주 | 101.9 | 105.2 | 105.3 | 102.4 | 100.6 | 103.8 | 102.6 | 101.8 |
2주 | 95.9 | 103.1 | 108.2 | 99.8 | 95.1 | 105.7 | 104.6 | 99.2 |
4주 | 102 | 104.4 | 107.6 | 101.2 | 104.4 | 108.2 | 102.1 | 103 |
8주 | 101.4 | 105.7 | 108.1 | 99.4 | 104.2 | 103.4 | 101.8 | 101.1 |
12주 | 104.1 | 108.2 | 107.1 | 103.6 | 106.9 | 104.1 | 103.1 | 104.9 |
결과 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 |
경과시간 | 저점도 유화 제형 (%) | |||||||
지페노사이드 UL4 | 지페노사이드 UL5 | |||||||
4℃ | 30℃ | 45℃ | 일광 | 4℃ | 30℃ | 45℃ | 일광 | |
1주 | 100.1 | 105.0 | 101.9 | 96.3 | 98.8 | 103.3 | 103.5 | 94.4 |
2주 | 102.4 | 101.8 | 106.2 | 99.5 | 100.4 | 103.9 | 102 | 98.1 |
4주 | 101.2 | 101.6 | 105.9 | 100.5 | 102.8 | 102.2 | 104.8 | 101.6 |
8주 | 94.9 | 104.1 | 95.6 | 99.8 | 100.2 | 105.6 | 94.2 | 101 |
12주 | 94.1 | 105.2 | 99.3 | 102 | 95.2 | 105 | 98.3 | 102.6 |
결과 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 |
경과시간 | 가용화 제형 (%) | |||||||
지페노사이드 UL4 | 지페노사이드 UL5 | |||||||
4℃ | 30℃ | 45℃ | 일광 | 4℃ | 30℃ | 45℃ | 일광 | |
1주 | 98.8 | 100.0 | 102.8 | 98.8 | 98.8 | 100 | 100.3 | 97.9 |
2주 | 91.9 | 103.2 | 101.6 | 94.8 | 95.7 | 105.1 | 101.4 | 95.9 |
4주 | 98.7 | 102.7 | 94.2 | 98.4 | 105.1 | 108.6 | 97.2 | 104.9 |
8주 | 97.6 | 98.5 | 97.2 | 98 | 102.8 | 103.7 | 102.1 | 103.5 |
12주 | 99.5 | 104.5 | 94.9 | 94.6 | 102.3 | 104.9 | 95.6 | 103.3 |
결과 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 | 안정 |
상 | 성분 | 함량 (%) |
수상 | 정제수 | To 100 |
보습성분 | 10 ~ 25 | |
점증제 | 적량 | |
금속이온 봉쇄제 | 적량 | |
유상 | 피이지-100스테아레이트 | 0.1 ~ 2 |
글리세릴스테아레이트 | 0.1 ~ 2 | |
폴리소르베이트 60 | 0.1 ~ 2 | |
스테아릭애씨드 | 0.1 ~ 2 | |
세테아릴알코올 | 0.1 ~ 2 | |
카프릭카프릴릭트리글리세라이드 | 10 ~ 30 | |
토코페릴아세테이트 | 0.1 ~ 0.5 | |
첨가 I | 칠엽담 지페노사이드 분획물 | 0.40 |
첨가 II | 향 | 적량 |
보존제 | 적량 | |
기타 첨가제 | 적량 |
Severity (중증도) | Wrinkle grading (주름 등급) |
0 | None (없음) |
1 | none / mild (없거나 약한 주름) |
2 | Mild (약한 주름) |
3 | mild / moderate (약하거나 중간 주름) |
4 | Moderate (중간 주름) |
5 | moderate / severe (중간이나 심한 주름) |
6 | Severe (심한 주름) |
7 | very severe (매우 심한 주름) |
상 | 성분 | 함량 ( % ) |
수상 | 정제수 | To 100 |
보습성분 | 10 ~ 25 | |
점증제 | 적량 | |
금속이온 봉쇄제 | 적량 | |
유상 | 피이지-100스테아레이트 | 0.1 ~ 2 |
글리세릴스테아레이트 | 0.1 ~ 2 | |
폴리소르베이트 60 | 0.1 ~ 2 | |
스테아릭애씨드 | 0.1 ~ 2 | |
세테아릴알코올 | 0.1 ~ 2 | |
카프릭카프릴릭트리글리세라이드 | 10 ~ 30 | |
토코페릴아세테이트 | 0.1 ~ 0.5 | |
첨가 I | 칠엽담 지페노사이드 분획물 | 0.40 |
첨가 II | 향 | 적량 |
보존제 | 적량 | |
기타 첨가제 | 적량 |
번호 | 나이 | 성별 | 위치 | 진피치밀도 (%) | |
도포 전 | 도포 4주 후 | ||||
1 | 29 | 여 | 좌 | 11.72 | 16.79 |
우 | 12.93 | 14.21 | |||
2 | 26 | 여 | 좌 | 16.81 | 14.63 |
우 | 15.51 | 14.31 | |||
3 | 25 | 여 | 좌 | 13.70 | 15.49 |
우 | 11.91 | 16.82 | |||
4 | 34 | 여 | 좌 | 18.41 | 20.29 |
우 | 17.61 | 19.27 | |||
5 | 28 | 여 | 좌 | 9.38 | 13.02 |
우 | 9.45 | 10.02 | |||
6 | 33 | 여 | 좌 | 17.52 | 18.05 |
우 | 15.86 | 17.46 | |||
7 | 32 | 여 | 좌 | 14.77 | 16.58 |
우 | 18.41 | 20.25 | |||
8 | 30 | 여 | 좌 | 9.23 | 9.49 |
우 | 10.64 | 15.82 | |||
9 | 26 | 여 | 좌 | 15.63 | 17.22 |
우 | 16.88 | 19.56 | |||
10 | 25 | 여 | 좌 | 15.36 | 17.79 |
우 | 16.56 | 17.89 | |||
평균값 | 14.41 | 16.25 | |||
표준편차 | 3.09 | 2.99 | |||
변화율 (%) | 12.77 (%) |
Claims (12)
- 칠엽담 지페노사이드 분획물을 포함하는 주름 개선용 화장료 조성물.
- 제 1항에 있어서,상기 칠엽담 지페노사이드 분획물은 하기 정의된 지페노사이드 UL1, 지페노사이드 UL2, 지페노사이드 UL3, 지페노사이드 UL4, 지페노사이드 UL5, 지페노사이드 UL6 및 지페노사이드 UL7으로 이루어진 군으로부터 선택된 하나 이상의 지페노사이드를 포함하는 것을 특징으로 하는 주름 개선용 화장료 조성물:상기에서,지페노사이드 UL1은 (23ξ)-3β,20β,21α-트리히드록시-19-옥소-21,23-에폭시-담마르-24-엔-3-O-[α-L-람노피라노실 (1→2)]-α-L-아라비노피라노사이드 (1-엔-3-O-[α아라비노피라노사이드 ((23ξ)-3β,20β,21α-trihydroxy-19-oxo-21,23-epoxy-dammar-24-ene-3-O-[α-L-rhamnopyranosyl (1→2)]-α-L-arabinopyranoside);지페노사이드 UL2는 (23ξ)-3β,20β,21α-트리히드록시-19-옥소-21,23-에폭시-담마르-24-엔-3-O-[β-D-글루코피라노실 (1→3)]-α-L-아라비노피라노사이드 (1실엔-3-O-[β아라비노피라노사이드 ((23ξ)-3β,20β,21α-trihydroxy-19-oxo-21,23-epoxy-dammar-24-ene-3-O-[β-D-glucopyranosyl (1→3)]-α-L-arabinopyranoside);지페노사이드 UL3은 (23ξ)-3β,20β,21α-트리히드록시-21,23-에폭시-담마르-24-엔-3-O-[α-L-람노피라노실 (1→2)][β-D-크실로피라노실 (1→3)]-α-L-아라비노피라노사이드 ((23ξ)-3β,20β,21α-trihydroxy-21,23-epoxy-dammar-24-ene-3-O-[α-L-rhamnopyranosyl (1→2)][β-D-xylopyranosyl (1opyranoLarabinopyranoside);지페노사이드 UL4는 ((23ξ)-3β,20β,21α-트리히드록시-19-옥소-21,23-에폭시-담마르-24-엔-3-O-[α-L-람노피라노실 (1→2)][β-D-크실로피라노실 (1→3)]-α-L-아라비노피라노사이드 ((23ξ)-3β,20β,21α-trihydroxy-19-oxo-21,23-epoxy- dammar-24-ene-3-O-[α-L-rhamnopyranosyl (1mnopyranoxylopyranosyl (1opyranoLarabinopyranoside);지페노사이드 UL5는 (23ξ)-3β,20β,21α-트리히드록시-19-옥소-21,23-에폭시-담마르-24-엔-3-O-[α-L-람노피라노실 (1→2)][β-D-글루코피라노실 (1→3)]-α-L-아라비노피라노사이드 ((23ξ)-3β,20β,21α-trihydroxy-19-oxo-21,23-epoxy- dammar-24-ene-3-O-[α-L-rhamnopyranosyl (1mnopyranoglucopyranosyl (1copyranLarabinopyranoside);지페노사이드 UL6은 3β,20S,21-트리히드록시-19-옥소-담마르-24-엔-3-O-[α-L-람노피라노실 (1→2)]{[β-D-크실로피라노실 (1→6)][β-D-글루코피라노실 (1→3)]}-α-L-아라비노피라노사이드-21-O-β-D-글루코피라노사이드 (3β,20S,21-trihydroxy-19-oxo-dammar-24-ene-3-O-[α-L-rhamnopyranosyl (1→2)]{[β-D-xylopyranosyl (1→6)][β-D-glucopyranosyl (1→3)]}-α-L-arabinopyranoside-21-O-β-D-glucopyranoside);지페노사이드 UL7은 3β,20S-디히드록시-21-카르복실-담마르-24-엔-3-O-[α-L-람노피라노실 (1→2)]{[β-D-크실로피라노실 (1→6)][β-D-글루코피라노실 (1→3)]}-α-L-아라비노피라노사이드-21-O-β-D-글루코피라노사이드 (3β,20S-dihydroxy-21-carboxyl-dammar-24-ene-3-O-[α-L-rhamnopyranosyl (1→2)]{[β-D-xylopyranosyl (1→6)][β-D-glucopyranosyl (1→3)]}-α-L-arabinopyranoside-21-O-β-D-glucopyranoside)를 의미한다.
- 제 1항 또는 제 2항에 있어서, 상기 칠엽담 지페노사이드 분획물은 총 조성물의 중량 대비 0.001 내지 30 중량%로 포함되는 것을 특징으로 하는 주름 개선용 화장료 조성물.
- 제 2항에 있어서, 상기 칠엽담 지페노사이드 분획물은 지페노사이드 UL4 및 지페노사이드 UL5 중 하나 이상을 총 분획물의 중량 대비 20 내지 30 중량%로 포함하는 것을 특징으로 하는 주름 개선용 화장료 조성물.
- 제 1항 또는 제 2항에 있어서, 상기 주름은 투명주름인 것을 특징으로 하는 주름 개선용 화장료 조성물.
- 제 6항에 있어서, 상기 조성물은 하기 정의된 지페노사이드 UL1, 지페노사이드 UL2, 지페노사이드 UL3, 지페노사이드 UL4, 지페노사이드 UL5, 지페노사이드 UL6 및 지페노사이드 UL7으로 이루어진 군으로부터 선택된 하나 이상의 지페노사이드를 포함하는 것을 특징으로 하는 주름 개선용 화장료 조성물:상기에서,지페노사이드 UL1은 (23ξ)-3β,20β,21α-트리히드록시-19-옥소-21,23-에폭시-담마르-24-엔-3-O-[α-L-람노피라노실 (1→2)]-α-L-아라비노피라노사이드 (1-엔-3-O-[α아라비노피라노사이드 ((23ξ)-3β,20β,21α-trihydroxy-19-oxo-21,23-epoxy-dammar-24-ene-3-O-[α-L-rhamnopyranosyl (1→2)]-α-L-arabinopyranoside);지페노사이드 UL2는 (23ξ)-3β,20β,21α-트리히드록시-19-옥소-21,23-에폭시-담마르-24-엔-3-O-[β-D-글루코피라노실 (1→3)]-α-L-아라비노피라노사이드 (1실엔-3-O-[β아라비노피라노사이드 ((23ξ)-3β,20β,21α-trihydroxy-19-oxo-21,23-epoxy-dammar-24-ene-3-O-[β-D-glucopyranosyl (1→3)]-α-L-arabinopyranoside);지페노사이드 UL3은 (23ξ)-3β,20β,21α-트리히드록시-21,23-에폭시-담마르-24-엔-3-O-[α-L-람노피라노실 (1→2)][β-D-크실로피라노실 (1→3)]-α-L-아라비노피라노사이드 ((23ξ)-3β,20β,21α-trihydroxy-21,23-epoxy-dammar-24-ene-3-O-[α-L-rhamnopyranosyl (1→2)][β-D-xylopyranosyl (1opyranoLarabinopyranoside);지페노사이드 UL4는 ((23ξ)-3β,20β,21α-트리히드록시-19-옥소-21,23-에폭시-담마르-24-엔-3-O-[α-L-람노피라노실 (1→2)][β-D-크실로피라노실 (1→3)]-α-L-아라비노피라노사이드 ((23ξ)-3β,20β,21α-trihydroxy-19-oxo-21,23-epoxy- dammar-24-ene-3-O-[α-L-rhamnopyranosyl (1mnopyranoxylopyranosyl (1opyranoLarabinopyranoside);지페노사이드 UL5는 (23ξ)-3β,20β,21α-트리히드록시-19-옥소-21,23-에폭시-담마르-24-엔-3-O-[α-L-람노피라노실 (1→2)][β-D-글루코피라노실 (1→3)]-α-L-아라비노피라노사이드 ((23ξ)-3β,20β,21α-trihydroxy-19-oxo-21,23-epoxy- dammar-24-ene-3-O-[α-L-rhamnopyranosyl (1mnopyranoglucopyranosyl (1copyranLarabinopyranoside);지페노사이드 UL6은 3β,20S,21-트리히드록시-19-옥소-담마르-24-엔-3-O-[α-L-람노피라노실 (1→2)]{[β-D-크실로피라노실 (1→6)][β-D-글루코피라노실 (1→3)]}-α-L-아라비노피라노사이드-21-O-β-D-글루코피라노사이드 (3β,20S,21-trihydroxy-19-oxo-dammar-24-ene-3-O-[α-L-rhamnopyranosyl (1→2)]{[β-D-xylopyranosyl (1→6)][β-D-glucopyranosyl (1→3)]}-α-L-arabinopyranoside-21-O-β-D-glucopyranoside);지페노사이드 UL7은 3β,20S-디히드록시-21-카르복실-담마르-24-엔-3-O-[α-L-람노피라노실 (1→2)]{[β-D-크실로피라노실 (1→6)][β-D-글루코피라노실 (1→3)]}-α-L-아라비노피라노사이드-21-O-β-D-글루코피라노사이드 (3β,20S-dihydroxy-21-carboxyl-dammar-24-ene-3-O-[α-L-rhamnopyranosyl (1→2)]{[β-D-xylopyranosyl (1→6)][β-D-glucopyranosyl (1→3)]}-α-L-arabinopyranoside-21-O-β-D-glucopyranoside)를 의미한다.
- 제 6항 또는 제 7항에 있어서, 상기 지페노사이드는 칠엽담으로부터 분리된 것임을 특징으로 하는 주름 개선용 조성물.
- 제 6항 또는 제 7항에 있어서, 상기 조성물은 총 조성물의 중량 대비 상기 지페노사이드를 0.0001 내지 30 중량%로 포함하는 것을 특징으로 하는 주름 개선용 화장료 조성물.
- 제 7항에 있어서, 상기 조성물은 총 조성물의 중량 대비 상기 지페노사이드 UL4 및 상기 지페노사이드 UL5 중 하나 이상을 0.0002 내지 9 중량%로 포함하는 것을 특징으로 하는 주름 개선용 화장료 조성물.
- 제 6항 또는 제 7항에 있어서, 상기 주름은 투명주름인 것을 특징으로 하는 주름 개선용 화장료 조성물.
- 제 1항 내지 제 11항 중 어느 한 항의 화장료 조성물을 사용하여 주름을 개선하는 방법.
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EP16807830.1A EP3308768B1 (en) | 2015-06-11 | 2016-06-10 | Cosmetic composition for wrinkle reduction containing gypenoside isolated from gynostemma pentaphyllum |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108774282A (zh) * | 2018-07-23 | 2018-11-09 | 中国科学院兰州化学物理研究所 | 一种绞股蓝中具有抗阿尔茨海默病的达玛烷型三萜皂苷 |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101642660B1 (ko) * | 2015-06-11 | 2016-07-25 | (주)셀트리온 | 칠엽담으로부터 분리된 지페노사이드를 포함하는 주름개선용 화장료 조성물 |
US10639343B2 (en) * | 2018-01-04 | 2020-05-05 | Btc Corporation | Preparation method of Gynostemma pentaphyllum leaves extract and Gynostemma pentaphyllum extract prepared by the method thereof |
KR102166253B1 (ko) * | 2018-10-22 | 2020-10-16 | 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 | 지페노사이드 75(Gypenoside LXXV)를 유효성분으로 포함하는 상처 치유용 조성물 |
KR102189376B1 (ko) | 2020-02-26 | 2020-12-11 | (주)셀트리온 | 칠엽담 추출물의 지표성분을 포함하는 항염증 또는 탈모방지용 조성물 |
CN112778392A (zh) * | 2021-01-05 | 2021-05-11 | 徐世明 | 一种绞股蓝化合物在抗肿瘤药物中的应用与制备 |
KR102298234B1 (ko) * | 2021-04-09 | 2021-09-06 | 주식회사 리베코스 | 효소 처리 돌외(Gynostemma Pentaphyllum) 및 호프(Humulus Lupulus) 혼합추출물을 유효성분으로 함유하는 주름개선 및 피부 탄력 증진용 화장료 조성물 |
KR102577747B1 (ko) | 2021-07-12 | 2023-09-12 | 한국해양과학기술원 | 남극 메켈 빙어 추출물 또는 남극 대리석무늬 암치 추출물을 유효성분으로 포함하는 피부 주름개선용 화장료 조성물 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100667448B1 (ko) * | 2005-09-01 | 2007-01-10 | 주식회사 쵸이스코스메틱 | 돌외 추출물이 함유된 화장료 조성물 |
KR20130069430A (ko) * | 2011-12-14 | 2013-06-26 | (주)셀트리온 | 돌외의 지페노사이드 추출물을 포함하는 제2형 당뇨병, 비만 또는 고지혈증의 치료 또는 예방용 조성물 |
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Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1102335A (zh) | 1993-11-05 | 1995-05-10 | 成都市中医医院 | 猕猴桃养身口服液 |
CN2194725Y (zh) | 1993-12-27 | 1995-04-19 | 夏春 | 去皱美容膏 |
CN1110132A (zh) | 1994-04-08 | 1995-10-18 | 拜忠信 | 抗皱祛斑美容霜及其制备方法 |
US5910308A (en) * | 1997-03-19 | 1999-06-08 | Sante International Inc. | Herbal extract composition containing gynostemma pentaphyllum, crataegus pinnatifida and camellia sinensis |
US6168795B1 (en) * | 1998-06-09 | 2001-01-02 | Sant{acute over (e)} International Inc. | Method for anticancer therapy using an herbal extract composition |
JP2003095858A (ja) | 2001-09-27 | 2003-04-03 | Nonogawa Shoji Kk | 皮膚外用剤 |
US20030124205A1 (en) * | 2001-12-28 | 2003-07-03 | Avon Products, Inc. | Topical cosmetic composition having a natural plant active ingredient and method of using same |
CN101076254B (zh) * | 2004-12-22 | 2012-10-31 | 雅芳产品公司 | 减轻皱纹外观的方法和组合物 |
US20060198809A1 (en) * | 2005-03-04 | 2006-09-07 | Woodridge Labs, Inc. | Gamma-amino butyric acid composition |
JP2007051091A (ja) * | 2005-08-18 | 2007-03-01 | Shiseido Co Ltd | ヒアルロン酸産生促進剤 |
KR100757175B1 (ko) | 2005-08-23 | 2007-09-07 | (주)아모레퍼시픽 | 녹차 유래의 캄페롤을 유효성분으로 함유하는 주름 개선용 및 피부 탄력 향상용 피부 외용제 조성물 |
KR100825450B1 (ko) | 2006-06-30 | 2008-04-28 | 주식회사 사임당화장품 | 연교추출물을 함유하는 피부 주름개선용 화장료 조성물 |
KR100844275B1 (ko) | 2006-12-20 | 2008-07-07 | 주식회사 코리아나화장품 | 노니 추출물을 유효성분으로 함유하는 피부노화 방지용화장료 조성물 |
CN103479694B (zh) | 2008-06-13 | 2016-06-29 | 株式会社爱茉莉太平洋 | 含有人参的花或人参的种子提取物的皮肤外用剂组合物 |
KR102016641B1 (ko) * | 2013-05-31 | 2019-08-30 | (주)아모레퍼시픽 | 포제 추출물을 포함하는 조성물 |
KR101642660B1 (ko) * | 2015-06-11 | 2016-07-25 | (주)셀트리온 | 칠엽담으로부터 분리된 지페노사이드를 포함하는 주름개선용 화장료 조성물 |
-
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100667448B1 (ko) * | 2005-09-01 | 2007-01-10 | 주식회사 쵸이스코스메틱 | 돌외 추출물이 함유된 화장료 조성물 |
KR20130069430A (ko) * | 2011-12-14 | 2013-06-26 | (주)셀트리온 | 돌외의 지페노사이드 추출물을 포함하는 제2형 당뇨병, 비만 또는 고지혈증의 치료 또는 예방용 조성물 |
KR20130075962A (ko) * | 2011-12-28 | 2013-07-08 | (주)미인 | 돌외추출물을 함유한 피부 진정 자외선 차단 기능성 화장료 조성물 |
Non-Patent Citations (3)
Title |
---|
HONG, HAO ET AL.: "Enzymatic Biotransformation of Ginsenoside Rb1 and Gypenoside XVII into Ginsenosides Rd and F2 by Recombinant B-glucosidase from Flavobacterium Johnsoniae", JOURNAL OF GINSENG RESEARCH, vol. 36, no. 4, October 2010 (2010-10-01), pages 418 - 424, XP053028942 * |
See also references of EP3308768A4 * |
TENG, RONG - WEI ET AL.: "Hydrolytic Reaction of Plant Extracts to Generate Molecular Diversity: New Dammarane Glycosides from the Mild Acid Hydrolysate of Root Saponins of Panax Notoginseng", HELVETICA CHIMICA ACTA, vol. 87, no. 5, 25 May 2004 (2004-05-25), pages 1270 - 1278, XP055335152 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108774282A (zh) * | 2018-07-23 | 2018-11-09 | 中国科学院兰州化学物理研究所 | 一种绞股蓝中具有抗阿尔茨海默病的达玛烷型三萜皂苷 |
CN108774282B (zh) * | 2018-07-23 | 2021-01-08 | 中国科学院兰州化学物理研究所 | 一种绞股蓝中具有抗阿尔茨海默病的达玛烷型三萜皂苷 |
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EP3308768A4 (en) | 2019-01-16 |
EP3308768A1 (en) | 2018-04-18 |
EP3308768B1 (en) | 2024-02-21 |
KR102229247B1 (ko) | 2021-03-18 |
KR101642660B1 (ko) | 2016-07-25 |
JP2020055852A (ja) | 2020-04-09 |
JP2018517725A (ja) | 2018-07-05 |
JP6828228B2 (ja) | 2021-02-10 |
US20190110980A1 (en) | 2019-04-18 |
KR20180008557A (ko) | 2018-01-24 |
US11007136B2 (en) | 2021-05-18 |
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