WO2009151212A2 - 인삼꽃 또는 인삼씨 추출물을 함유하는 피부 외용제 조성물 - Google Patents
인삼꽃 또는 인삼씨 추출물을 함유하는 피부 외용제 조성물 Download PDFInfo
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- WO2009151212A2 WO2009151212A2 PCT/KR2009/002206 KR2009002206W WO2009151212A2 WO 2009151212 A2 WO2009151212 A2 WO 2009151212A2 KR 2009002206 W KR2009002206 W KR 2009002206W WO 2009151212 A2 WO2009151212 A2 WO 2009151212A2
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/06—Antipsoriatics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/12—Keratolytics, e.g. wart or anti-corn preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/006—Antidandruff preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/12—Preparations containing hair conditioners
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
Definitions
- the present invention relates to the use of ginseng flower extract and the use of ginseng seed extract.
- Ginseng seed extract includes ginseng seed oil or ginseng seed cake extract. More specifically, the present invention is a skin protection use of ginseng flower extract or ginseng seed extract, anti-aging use, anti-wrinkle use, skin elasticity improvement use, whitening use, skin moisturizing use, prevention and improvement of skin dry diseases such as atopy use
- anti-inflammatory, prevention or improvement of skin troubles such as acne, exfoliation, skin color improvement, hair loss prevention, antidandruff, hair gloss and softness improvement, volume and elasticity of face or body, face or body It is about improving the line of the body.
- Ginseng (Panax ginseng C.A. Meyer) is a plant belonging to the genus Ogapi and ginseng, and has been used in Korea, China and Japan for more than 2,000 years, and has been used to prevent disease and extend life.
- Ginseng's known effects and effects to the central nervous system, anti-carcinogenic and anti-cancer activity, immune function regulation, anti-diabetic effect, liver function anti-inflammatory effect, cardiovascular disorder improvement and anti-arteriosclerosis, blood pressure control action, menopausal Effects on improving disorders and osteoporosis, antistress and anti-fatigue, antioxidant activity and anti-aging effects are known.
- Ginsenoside a representative physiologically active component of ginseng, is evenly distributed on the ground and underground parts of ginseng.
- ginsenoside content and composition differ greatly depending on the parts of ginseng root (root), ginseng leaf, ginseng fruit.
- ginseng flowers have been reported to have high hepatoprotective efficacy unlike other parts based on ginseng root (root) and other ginsenoside components and ingredients (J. Nat. Prod, 66; 922-927, 2003.) .
- ginseng is also being researched as a plant material having important skin effects of cosmetics.
- most of them use ginseng root. Specifically, only ginsenoside components and ginseng polysaccharides of the extract or ginseng root of ginseng root, using the ginseng flower or ginseng seed component of the skin efficacy is never studied.
- An object of the present invention is to provide a skin external preparation useful for skin.
- An object of one embodiment of the present invention to provide a composition for protecting the skin.
- Another object of an embodiment of the present invention to provide a composition for improving skin elasticity.
- Another object of one embodiment of the present invention to provide a composition for skin whitening.
- Another object of one embodiment of the present invention to provide a composition for moisturizing the skin.
- Another object of one embodiment of the present invention to provide a composition for preventing or improving skin dry diseases such as atopy.
- Another object of an embodiment of the present invention is to provide a composition for controlling sebum of the skin.
- Another object of one embodiment of the present invention to provide a composition for preventing or improving skin problems such as acne.
- Another object of an embodiment of the present invention is to provide a composition for preventing or improving skin troubles such as acne occurring on the scalp.
- Another object of an embodiment of the present invention is to provide a composition for preventing hair loss.
- Another object of one embodiment of the present invention to provide a composition for anti-dandruff.
- the external preparation composition for skin according to the present invention contains ginseng flower extract or ginseng seed extract as an active ingredient.
- the ginseng seed extract may be ginseng seed oil.
- the content thereof is preferably 0.001 to 100% by weight based on the total weight of the skin external preparation composition.
- the ginseng seed extract may be a ginseng seed cake extract.
- the content of the ginseng seed cake extract is preferably 0.001 to 10% by weight based on the total weight of the external skin composition.
- the external preparation composition for skin is characterized in that it has a use for protecting skin by inhibiting skin damage.
- the skin here includes the scalp or hair. That is, in the present specification, a topical skin composition for protecting hair by inhibiting damage to the scalp or hair is disclosed.
- the external preparation composition for skin is characterized by protecting the skin through the action of inhibiting skin damage caused by oxidation or ultraviolet rays.
- the skin here includes the scalp or hair. That is, the external composition for skin to protect the scalp or hair by inhibiting damage to the scalp or hair by oxidation or ultraviolet rays is disclosed.
- the external preparation composition for skin is characterized by having a use for preventing or improving skin aging.
- the external preparation composition for skin aging may be caused by inhibiting skin damage caused by oxidation or ultraviolet rays, promoting collagen production or inhibiting biosynthesis of matrix metalloproteinase I (MMP-1). It is characterized by suppressing.
- MMP-1 matrix metalloproteinase I
- the external preparation composition for skin according to another embodiment of the present invention is characterized by having a use for preventing or improving skin wrinkles.
- the external preparation composition for skin according to another embodiment of the present invention is characterized by preventing or improving skin wrinkles by promoting collagen production or inhibiting biosynthesis of matrix metalloproteinase I (MMP-1).
- MMP-1 matrix metalloproteinase I
- the external preparation composition for skin according to another embodiment of the present invention is characterized by having a use for improving skin elasticity.
- the external preparation composition for skin according to another embodiment of the present invention is characterized by having a skin whitening use.
- the external preparation composition for skin according to another embodiment of the present invention is characterized in that the skin whitening is performed through inhibiting melanin production or whitening of the pigment deposited on the skin.
- the external preparation composition for skin according to another embodiment of the present invention has a skin moisturizing use.
- the external preparation composition for skin according to another embodiment of the present invention is characterized by maintaining skin moisture through promoting differentiation of keratinocytes or promoting expression of transglutaminase.
- the external preparation composition for skin according to another embodiment of the present invention is characterized by having a use for preventing or ameliorating a skin dry disease including atopy or psoriasis.
- the external preparation composition for skin is used for exfoliation.
- the external preparation composition for skin according to another embodiment of the present invention is characterized by having a sebum control use of the skin.
- the external preparation composition for skin according to another embodiment of the present invention is characterized by controlling sebum of the skin by inhibiting secretion of sebum.
- the external preparation composition for skin according to another embodiment of the present invention is characterized by having the use of preventing or ameliorating inflammation or alleviating irritation.
- the skin contains the scalp. That is, the present invention provides an external composition for skin having a use for preventing or ameliorating inflammation of the scalp and alleviating irritation to the scalp.
- the external preparation composition for skin according to another embodiment of the present invention is characterized by having a use for preventing or improving skin troubles including acne.
- the skin contains the scalp. That is, the present specification provides an external composition for skin having a use for preventing skin troubles including acne from occurring on the scalp and improving skin troubles.
- the external preparation composition for skin is characterized by having a use for improving skin color.
- the external preparation composition for skin is characterized by having a purpose of preventing hair loss.
- the external preparation composition for skin is characterized by having anti-dandruff use.
- the external preparation composition for skin is characterized by having a use for enhancing the gloss or softness of hair.
- the external preparation composition for skin is characterized in that it has a use to enhance the volume and elasticity of the face or body.
- the external preparation composition for skin is characterized in that it has a use to improve the lines of the face or body.
- the present invention by containing the ginseng flower extract or ginseng seed extract having a different component and composition from the ground portion of ginseng as an active ingredient, skin protection, anti-aging, anti-wrinkle, skin elasticity improvement, whitening, skin moisturizing, atopy, etc.
- Prevention and improvement of dry skin disease, exfoliation, sebum control, anti-inflammatory, prevention or improvement of skin troubles such as acne, improvement of skin complexion, prevention of hair loss, anti dandruff, improvement of gloss and softness of hair, volume and elasticity of face or body
- 1 is a graph showing the results of the inhibition test of the active oxygen production of ginseng flower extract according to an embodiment of the present invention.
- Figure 2 is a graph showing the DNA oxidative damage inhibitory activity of the ginseng flower extract according to an embodiment of the present invention.
- Figure 3 is a graph showing the effect of ginseng flower extract according to an embodiment of the present invention on the production of type I procollagen.
- Figure 4 is a graph showing the effect of the ginseng flower extract according to an embodiment of the present invention on the expression of MMP-1.
- 5 is a graph showing the effect of ginseng flower extracts on melanin production according to an embodiment of the present invention.
- Figure 6 is a graph showing the melanin production inhibitory effect by the ginseng seed extract according to the present invention.
- extract includes any material obtained by extracting a component therefrom from a natural product, regardless of the method of extraction or the kind of the component.
- extract includes all components obtained by extracting a component dissolved in a solvent from a natural product using water or an organic solvent, and those obtained by extracting only a specific component such as an oil such as oil.
- ginseng seed oil means an oil obtained from seeds of ginseng.
- it may be an extract extracted from ginseng seed using an organic solvent such as hexane, ether, methylene chloride and the like.
- ginseng seed refers to the remaining portion from which oil is removed from ginseng seed.
- organic solvent such as hexane, ether, methylene chloride, it may be the remainder remaining after extracting the oil from ginseng seed.
- skin refers to a tissue covering the body surface of an animal, and is a broad concept including not only tissues covering the body surface such as the face or body, but also the scalp and hair.
- the ginseng is Panax ginseng C.A. Meyer.
- the ginseng flower extract may be mixed in an amount of 0.001 to 10% by weight based on the total weight of the composition, but is not limited thereto.
- Ginseng flower extract according to an embodiment of the present invention, water, dried organic ginseng flower, including an organic solvent (ethanol, methanol, butanol, ether, ethyl acetate, chloroform, etc.) or organic solvent 1 to 10 times It can be prepared by adding (wt / v%) and standing at room temperature for one day. Preferably, the above process is repeated twice to obtain an extract. Further, preferably, after removing chlorophyll from the extract, the filtrate can be concentrated in a vacuum concentrator to obtain a dried product of ginseng flower extract.
- an organic solvent ethanol, methanol, butanol, ether, ethyl acetate, chloroform, etc.
- organic solvent 1 to 10 times it can be prepared by adding (wt / v%) and standing at room temperature for one day.
- the above process is repeated twice to obtain an extract.
- the filtrate can be concentrated in a vacuum concentrator to obtain a dried product of ginseng flower extract.
- the ginseng seed extract is ginseng seed oil
- the oil may be mixed in an amount of 0.001 to 100% by weight based on the total weight of the composition, depending on the form of the skin external preparation composition. If the ginseng seed oil is less than 0.001% by weight because the effect is insignificant.
- the ginseng seed oil is pulverized dried ginseng seed, and then put an organic solvent (hexane, ether, methylene chloride, etc.) 1 to 10 times (wt / v%) of the crushed It can be extracted by leaving it for one day at room temperature. For example, the above process is repeated three times to obtain an extract, the extract is filtered, and then the filtrate is concentrated in a vacuum concentrator to obtain ginseng seed oil.
- an organic solvent hexane, ether, methylene chloride, etc.
- the ginseng seed cake extract may be mixed in an amount of 0.001 to 10% by weight based on the total weight of the composition, depending on the form of the external preparation for skin. If the ginseng seed cake extract is less than 0.001% by weight, the effect is insignificant, if it exceeds 10% by weight because the increase in effect is not large compared to the increase in content.
- the ginseng seed cake extract may be prepared by the following method. After crushing the dried ginseng seed, 1-10 times (wt / v%) of an organic solvent, such as hexane, is added and extracted by standing at room temperature for one day. Repeat the above process three times to remove oil from ginseng seed, and remove organic oil (ethanol, methanol, butanol, ether, ethyl acetate, chloroform, etc.) or organic solvent in ginseng seed cake after oil is removed. 1 to 10 times (wt / v%) and put at room temperature for one day can be extracted. The above process is repeated three times to obtain an extract, and the extract is filtered, and then the filtrate is concentrated in a vacuum concentrator to obtain a dried product of ginseng seed cake extract.
- an organic solvent such as hexane
- Human skin is changed by a number of internal and external factors as it ages. That is, internally, the secretion of various hormones that regulate metabolism decreases, and the function of immune cells and the activity of cells decreases, thereby reducing the biosynthesis of immune proteins and constituent proteins necessary for living organisms. Due to the increase in the amount of ultraviolet rays reaching the surface of the sun's rays and further increase environmental pollution, free radicals and free radicals, such as free radicals, skin thickness, wrinkles, elasticity is reduced only In addition, the color of the skin becomes dull, skin problems frequently occur, and blemishes, freckles, and mushrooms are also increasing.
- cyclooxygenase-2 (Cox-2, cyclooxygenase), an enzyme that produces proinflammatory cytokine, which is known to cause inflammation, in most cells constituting the skin due to aging or ultraviolet rays.
- MMP matrix metalloproteinase
- the biosynthesis of the substrate material is reduced by the reduction of cellular activity and micro-inflammation due to naturally occurring endogenous aging, and by external factors such as the increase of stress caused by various harmful environments and the increase of free radical species caused by sunlight.
- decomposition and degeneration are accelerated, and the skin matrix is destroyed and thinned, resulting in various symptoms of skin aging.
- the external application composition for skin disclosed herein includes ginseng flower extract or ginseng seed extract as an active ingredient that inhibits the production of free radicals and inhibits oxidative damage caused by free radicals. Damage to the cells can be prevented and the skin protected. In addition, the progress of aging due to active oxygen or ultraviolet rays can be suppressed. In addition, ginseng flower extract and ginseng seed extract can prevent or improve inflammation by inhibiting the biosynthesis of COX-2, thereby alleviating skin problems.
- the skin comprises the scalp. That is, the ginseng flower extract or ginseng seed extract disclosed herein prevents damage to cells caused by free radicals or ultraviolet rays generated from the scalp or hair, prevents or improves inflammation of the scalp, It can be used to alleviate irritation. In addition, the ginseng flower extract or ginseng seed extract disclosed herein may be used for the purpose of preventing or improving wrinkles of the skin.
- melanocytes melanocytes
- the distribution of blood vessels the thickness of skin
- pigments in the body such as carotenoids and bilirubin. It is important.
- melanin a black pigment which is produced by the action of various enzymes such as tyrosinase in melanocytes in the human body.
- the formation of this melanin pigment is influenced by genetic factors, physiological factors related to hormone secretion, stress, and environmental factors such as ultraviolet irradiation.
- Melanin pigment which is produced from melanocytes of the skin of the body, is a phenolic polymer material having a complex form of black pigment and protein. It protects skin organs below the dermis by blocking ultraviolet rays from the sun and free radicals generated in the skin body. It plays a useful role in protecting proteins and genes in the skin.
- melanin produced by stress stimulation inside and outside the skin is a stable substance that does not disappear until it is discharged to the outside through skin keratinization even if the stress disappears.
- it causes hyperpigmentation such as blemishes, freckles, and spots, resulting in cosmetically bad results.
- the ginseng flower extract or ginseng seed extract disclosed herein can whiten the skin by inhibiting the production of melanin. Therefore, the external preparation composition for skin disclosed herein containing ginseng flower extract or ginseng seed extract as an active ingredient may be used for skin whitening.
- the most important function of the outermost epidermis of the skin is to protect the skin from various external stimuli (chemicals, air pollutants, dry environment, physical and chemical stimulating factors such as ultraviolet rays). It is a protective function that prevents excessive divergence of moisture through the body, and this protective function can be maintained only when the stratum corneum composed of keratinocytes is normally formed.
- the outermost stratum corneum (horney layer) of the epidermis is formed from keratinocytes and is composed of the differentiated keratinocytes and the surrounding lipid layer. Keratinocytes are characteristic cells whose basal cells, which continuously proliferate in the epidermal layer, undergo morphological and functional changes in stages and rise to the surface of the skin.
- keratinization keratinocytes form the stratum corneum by generating natural moisturizing factor (NMF) and intercellular fat (ceramide, cholesterol, fatty acids), making the stratum corneum firm and flexible. It has a function as).
- NMF natural moisturizing factor
- ceramide, cholesterol, fatty acids intercellular fat
- the turnover rate of the stratum corneum is slowed, the lipid synthesis ability of the keratinocytes is degraded, or the epidermis is normal in the epidermis. Due to poor cell division, maturation and differentiation, the amount of moisturizing factors and lipids in the stratum corneum is reduced, so that humans with skin with a condition that is unable to maintain normal stratum corneum function, that is, the skin barrier function is deteriorated There is a growing trend.
- the skin Due to the abnormal division and differentiation of epidermal cells, the skin causes various skin diseases such as dry skin, atopy and psoriasis, and these diseases can alleviate the symptoms slightly by using a conventional moisturizer having only water retention function. However, it is difficult to expect radical healing.
- the ginseng flower extract or ginseng seed extract disclosed in the present specification promotes the differentiation of keratinocytes, thereby undergoing the process of ⁇ epidermal cell differentiation '' or ⁇ keratinization '', a process of eliminating old keratinocytes and producing new keratinocytes. And thus have the effect of effectively removing dead skin cells.
- the external preparation composition for skin disclosed herein may be usefully used as a composition for exfoliation by containing ginseng flower extract or ginseng seed extract having an excellent exfoliation efficacy as an active ingredient.
- the ginseng flower extract or ginseng seed extract disclosed herein can maintain the amount of moisturizing factors and lipids in the stratum corneum by maintaining the turn-over rate of the stratum corneum in the normal range. It also promotes trans glutamine expression. Through this, it is possible to moisturize the skin.
- the ginseng flower extract or ginseng seed extract disclosed herein can enhance the skin barrier function by promoting the differentiation of keratinocytes and maintaining skin moisture, thereby preventing or preventing skin dry diseases such as atopy or psoriasis. It can be cured.
- the sebum in the skin including the scalp and face plays a role in maintaining the moisturizing of the skin and preventing the invasion of microorganisms, but excessive secretion of sebum promotes hair loss, worsens acne, promotes the enlargement of pores by acne, and seborrheic dermatitis.
- sebum in the skin including the scalp and face plays a role in maintaining the moisturizing of the skin and preventing the invasion of microorganisms, but excessive secretion of sebum promotes hair loss, worsens acne, promotes the enlargement of pores by acne, and seborrheic dermatitis.
- Sebum hypersecretion is caused by a number of causes, the most important of which is the sebaceous gland cells are activated by the amount of dihydrotestosterone (DHT), one of the hormones involved in promoting sebum secretion in sebaceous gland activity Is oversecreted. That is, in hair loss, testosterone (T) is converted into dihydrotestosterone, which is one of the male hormones, by 5-alpha-reductase type 2 in the cell, and at the same time, a receptor in the cytoplasm.
- DHT dihydrotestosterone
- testosterone binds to the protein and enters the fluid, causing hair loss, but in the skin or sebaceous glands, testosterone is converted to dihydrotestosterone by 5-alpha-reductase type 1 to activate sebaceous gland cells. Differentiation is promoted by oversecreting sebum in the sebaceous glands, causing acne.
- Ginseng flower extract or ginseng seed extract disclosed herein can prevent or improve acne, pores enlargement, seborrheic dermatitis and the like caused by excessive secretion of sebum.
- the external application composition for skin disclosed herein may enhance the volume and elasticity of the face and body by promoting the differentiation of fat cells.
- volume and elasticity are enhanced.
- Sites in which volume and elasticity enhancement are desired may be sites recessed due to aging. For example, the cheek meat etc. depressed by aging are mentioned. Applying the topical skin composition disclosed herein to the depressed cheeks, the volume can be enhanced by promoting adipocyte differentiation.
- areas where volume and elasticity enhancement are required include chest and hip areas. Applying the skin topical composition disclosed herein to the chest and buttocks has the effect of making the chest and buttocks elastic and voluminous.
- topical skin composition disclosed herein may improve the lines of the face and body.
- the ginseng flower extract or ginseng seed extract contained as an active ingredient promotes the differentiation of fat cells to promote the volume and elasticity of the face and body, promote the biosynthesis of collagen and inhibit the breakdown of collagen, It can improve the lines of damaged face and body.
- the cosmetic form according to the invention contains a cosmetically or dermatologically acceptable medium or base.
- a cosmetically or dermatologically acceptable medium or base are all formulations suitable for topical application, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) obtained by dispersing the oil phase in solutions, gels, solids, pasty anhydrous products, aqueous phases, and It may be provided in the form of a nonionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or cone stick.
- These compositions can be prepared according to conventional methods in the art.
- the composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.
- Cosmetics of the present invention may be used in the preparation of fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers.
- Cosmetics such as fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics or It may contain adjuvants commonly used in the field of dermatology. Such adjuvants are introduced in amounts generally used in the cosmetic or dermatological arts.
- the external preparation for skin containing the ginseng flower extract or ginseng seed extract of the present invention is not particularly limited in the formulation, for example, supple cosmetics, astringent cosmetics, nourishing cosmetics, nourishing cream, massage cream, essence, eye cream, It may be formulated into cosmetics such as eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil and body essence.
- the external preparation composition for skin according to the present invention may be a pharmaceutical composition.
- the pharmaceutical composition may further contain pharmaceutical adjuvants such as preservatives, stabilizers, hydrating or emulsifying accelerators, salts for regulating osmotic pressure and / or buffers and other therapeutically useful substances.
- the pharmaceutical composition may be formulated as a lotion, cream, ointment or gel.
- the pharmaceutical composition is preferably administered transdermally.
- Dosage of ginseng flower extract or ginseng seed extract as an active ingredient of the pharmaceutical composition is the age, sex, weight of the subject to be treated, the specific disease or pathology to be treated, the severity of the disease or pathology, the route of administration and the judgment of the prescriber Will depend on. Dosage determination based on these factors is within the level of skill in the art. Generally dosages range from 0.0001 mg / kg / day to approximately 2000 g / kg / day.
- the antioxidant effects of ginseng flower extract and ginseng seed extract were investigated by comparing the ability to remove reactive oxygen species (ROS) generated from cells by ultraviolet (UV) irradiation.
- ROS reactive oxygen species
- vitamin C As a positive control of ginseng flower extract, vitamin C (Sigma. Co.), which is generally used to compare antioxidant effects, was used.
- the group not treated with the test substance was used as a control.
- the extract prepared according to Example 1 was treated with ultraviolet (UVB 30 mJ / cm 2 ) in a human HaCaT keratinocytes monolayer culture system (P Boukamp, German Cancer Research Center, Heidelberg.German). 10 ppm, 50 ppm and 100 ppm were respectively treated.
- DCFH-DA (2 ', 7'-dichlorodihydro-fluorescein diacetate, Molecular Probes, Inc) was measured by a fluorescent plate reader.
- the control group not treated with UV was set to 100 and the ratio of ROS generation was calculated as a ratio thereof. The results are shown in Table 1 and FIG.
- the ginseng flower extract prepared according to Example 1, the ginseng seed oil prepared according to Example 2 and the ginseng seed cake extract of Example 3 are the controls in the human HaCaT keratinocytes monolayer culture system. Compared with the present invention, the active oxygen produced by UV was significantly removed. This is a good activity similar to that of vitamin C or trolox, which is used as an indicator of antioxidant activity.
- the ginseng flower extract prepared according to Example 1 of the present invention, the ginseng seed oil prepared according to Example 2 and the ginseng seed park extract of Example 3 by significantly scavenging the active oxygen causing skin damage It has been confirmed that it may have an effect of protecting the skin including the scalp and hair and further preventing wrinkle formation, elasticity reduction and pigmentation.
- ginseng flower extract of Example 1 After culturing normal human fibroblasts (primary culture) for 24 hours, ginseng flower extract of Example 1, ginseng seed oil prepared according to Example 2, ginseng seed cake extract and vitamin E of Example 3 After 12 hours, the positive control group was treated with H 2 O 2 (final concentration 10-3M) and stained by electrophoresis. At this time, the length (um) of the tail resulting from DNA cleavage in each of 25 cells was analyzed using KOMET 3.1 (Kinetic Imaging, England), an image analyzer. Table 3, And shown in FIG. 2.
- ginseng flower extract prepared according to Example 1 by cell gel electrophoresis and ginseng seed oil of Example 2 has a significant oxidative DNA damage inhibitory activity
- the ginseng flower extract or ginseng seed extract according to the present invention acts as a DNA oxidative damage inhibitor that inhibits DNA single strand break caused by hydroxyl radical (.OH). .
- This is an important protective effect of protecting the genes of cells of the skin, including the scalp or hair.
- Human fibroblasts were cultured in a 12-hole plate incubator at a concentration of 10 5 , followed by a medium containing 1 ppm, 10 ppm, and 50 ppm of ginseng flower extract of Example 1, ginseng seed oil of Example 2 or 1 ppm of ginseng seed cake extract of Example 3 , was replaced with a medium containing 10ppm as Table 4.
- Cells were harvested on the third day of culture to quantify the amount of type I procollagen produced by ELISA.
- TGF- ⁇ Human Transforming Growth Factor- ⁇ 1, Roche Co.
- the results were calculated as a comparison with the values measured with the control group not including ginseng flower extract and ginseng seed extract as 0 and the positive control group as 100. This is shown in Table 4 and FIG.
- the composition for external application for skin containing the ginseng flower extract or ginseng seed extract according to the present invention may promote collagen production to alleviate collagen reduction caused by aging of human skin.
- Human fibroblasts were cultured in a 12-hole plate incubator at a concentration of 10 5 and then irradiated with ultraviolet B at 40 mJ / cm 2 , and then replaced with a medium containing 1 ppm, 10 ppm and 50 ppm of ginseng flower extract of Example 1.
- Cells were harvested on the second day of culture (harvest) to quantify the amount of matrix metalloproteinase I (MMP-1) produced by ELISA. The result was calculated as a comparison with the value measured using a control group not irradiated with UV light containing no test substance 100, the positive control group was used RA (Retinoic acid , Sigma. Co.). This is shown in Table 5 and FIG.
- Example 5 For 120 subjects with facial wrinkles aged 30 to 50 years, the cream containing the ginseng flower extract of Example 1 having the composition shown in Table 6 and the ginseng seed oil of Example 2 Nutritional cream containing ginseng seed cake extract of Example 3 (Example 5), nutritional cream of the same composition (Comparative Example 1) as in Examples 4 and 5, except that the ginseng flower extract and ginseng seed extract After the preparation of the skin wrinkles improvement effect was compared (unit: weight%).
- Example 5 Comparative Example 1 Ginseng flower extract 0.1 - - Ginseng seed oil - 0.1 - Ginseng Seed Extract - 0.1 - Beeswax 10 10 10 Polysorbate 60 1.5 1.5 1.5 Sebum 60 Cured Castor Oil 2 2 2 Sorbitan sesquioleate 0.5 0.5 0.5 Liquid paraffin 10 10 10 Squalane 5 5 5 Caprylic / Capric Triglycerides (Estasan: Uniqema) 5 5 5 glycerin 5 5 5 5 Butylene glycol 3 3 3 3 Propylene glycol 3 3 3 3 Triethanolamine 0.2 0.2 0.2 0.2 Preservative, coloring, flavoring Quantity Quantity Quantity Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount
- Example 4 or 5 for the facial left side and Comparative Example 1 for the right side for 3 months, respectively.
- the skin condition was measured on both sides of the face before the use of the cream, and three months after the use of the cream, the same area was remeasured to measure the change in skin wrinkles.
- the wrinkles at the tail of the eyes were replicated, and skin wrinkles were measured using a Visiometer system (C + K).
- the amount of change in skin wrinkles was calculated according to Equation 1 below.
- Equation 1 "Tdi” is a measurement site value at D90, and “Tdo” is a measurement site value at D0.
- the skin wrinkles of the region using Comparative Example 1 showed a decrease of 6.1 ⁇ 7% (mean ⁇ standard deviation), while the skin wrinkles of the region using Example 4 were 35 ⁇ .
- the skin wrinkles of the site using 8% and Example 5 showed a 32 ⁇ 5% decrease, indicating an excellent skin wrinkle improvement effect.
- the skin elasticity improving effect of Examples 4, 5 and Comparative Example 1 prepared by the composition of Table 6 was measured.
- the skin elasticity tester (Cutometer SEM 575, C +) was divided into three sets of 60 women over 30 years of age, and applied to each group of the nutrition creams of Examples 4 or 5 and Comparative Example 1 twice a day for 12 weeks. Skin elasticity was measured using K Electronic Co., Germany).
- the elasticity effect of the site using Comparative Example 1 showed 0.21 ⁇ 0.14 (mean ⁇ standard deviation), while the elasticity effect of the site using Example 4 was 0.30 ⁇ 0.10, the site using Example 5
- the elasticity effect of showed 0.29 ⁇ 0.08, showing an excellent skin elasticity improvement effect.
- the result value refers to the nature of the skin viscoelasticity of the skin elasticity measuring instrument.
- murine Mel-Ab cells derived from C57BL / 6 mice (Dooley, TP et al, Skin pharmacol, 7, pp 188-200) were placed in DMEM (Dulbeccos modified Eagles media) in 10% fetal placental serum, 100 nM.
- the culture was carried out at 37 ° C. and 5% CO 2 in a medium to which 2-O-tetracanocanophorbol-13-acetate and 1 nM cholera toxin were added.
- the cultured Mel-Ab cells were detached with 0.25% trypsin-EDTA, incubated at a concentration of 10 5 cells / well in 24-well plates, and 10 ppm and 50 ppm, respectively, for 2 consecutive days from 2nd day.
- ginseng flower extract (Example 1), ginseng seed oil (Example 2) and ginseng seed gourd extract (Example 3) was added and cultured.
- arbutin arbutin, Sigma Co.
- the culture solution was removed, washed with PBS, the cells were dissolved with 1N sodium hydroxide, and the absorbance was measured at 400 nm.
- the melanin production inhibition rate was calculated according to Equation 2 below.
- FIG. 6 Dooley's method).
- Example 1 Example 2
- Example 3 Untreated group 0 0 0 10 ppm treatment group 4 3 23 50 ppm treatment group 25 19 32 100 ppm treatment group 49 43 51 Arbutin 100 ppm 51
- ginseng seed oil of Example 2 was carried out as follows.
- UVB ultraviolet light
- the ginseng flower extract of Example 1 After the UV irradiation, the ginseng flower extract of Example 1, the ginseng seed oil of Example 2, the ginseng seed cake extract of Example 3 and hydroquinone were applied to each of the test substance, and the place was changed for 10 weeks without applying anything. Was observed. Skin color was measured on a weekly basis with a colorimeter CR2002 (Minolta, Japan).
- the difference in skin color ( ⁇ L *) at the start of the application and the completion of the application of each test substance was calculated according to Equation 3 below, and the results are shown in Table 8 below.
- the whitening effect is determined by comparing ⁇ L * between the sample application site and the control site. When ⁇ L * value is about 2, the whitening effect of the deposited pigment is clear, and when it is about 1.5 or more, the whitening effect is determined. Can be.
- the ginseng flower extract of Example 1 confirmed that the skin color brightness of a similar degree to hydroquinone It was. This is because the ginseng flower extract, ginseng seed oil and ginseng seed gourd extract improve the pigmentation produced by ultraviolet light to brighten the skin color.
- CE Corel Envelop
- HaCaT human skin cell lines
- test substances described in Table 9 below were added to the culture medium by concentration, and 5 days until the cells grew to 70-80% of the floor area. Incubated. The cells were harvested and washed with PBS (phosphate buffered saline), followed by 10 mM Tris-HCl buffer (Tris-HCl) containing 2% Sodium Dodecyl Sulfate (SDS) and 20 mM Dithiothreitol (DTT). , pH 7.4) 1 ml was added, and the precipitates sonicated, boiled, and centrifuged were again suspended in 1 ml of PBS to measure absorbance at 340 nm.
- PBS phosphate buffered saline
- Tris-HCl Tris-HCl buffer
- DTT Dithiothreitol
- ginseng flower extract of Example 1 ginseng seed oil of Example 2 and ginseng seed cake extract of Example 3 was confirmed to promote differentiation in keratinocytes. This suggests that skin moisturization can be enhanced by normalizing the turnover cycle, which is the elimination of old keratinocytes and the production cycle of new keratinocytes.
- ginseng flower extract, ginseng seed oil and ginseng seed cake extract can promote skin exfoliation.
- Human keratinocytes 5 ⁇ 10 in each well of a 96 well cell culture plate 4 The dog was placed and attached for 24 hours. After treatment with the test substance to the attached skin cell line, two days later, the medium was removed, and stored in a -20 °C refrigerator. After freeze-thawing was repeated twice, the cells treated with material were destroyed, and then treated with acetone: ethanol (1: 1, v / v) stored at -20 ° C and left at 4 ° C for 30 minutes. The cells were fixed.
- the ginseng flower extract of Example 1 according to the present invention showed an excellent expression compared to the control. Therefore, it was found that the ginseng flower extract of Example 1 according to the present invention improves the expression of transglutaminase.
- Example 4 Example 5 and Comparative Example 1 were applied to the face twice daily for four weeks in each group.
- constant temperature, constant humidity 24 ° C, 40% relative humidity
- Skin moisture was measured and the results are shown in Table 11 below. The test result is a percentage of the increase of the measured value after a certain period of treatment based on the Koniometer value measured immediately before the test was started.
- the Koniometer is a skin moisture meter that measures the amount of moisture present on the skin by measuring the conductivity of the epidermis.
- Table 11 the ginseng seed oil of Example 4, Example 2 containing the ginseng flower extract of Example 1 and the material of Example 5 containing the ginseng seed cake extract of Example 3 Compared with the control group to which Comparative Example 1 was applied, the skin moisture content was increased in the group. Also, two weeks after the application of the nourishing creams of Examples 4 and 5 (for a total of six weeks), the value of the skin moisture measured was similar to that after 1 to 2 weeks. It was found that even without applying Example 5, the skin moisture by the application of the ginseng flower extract, ginseng seed oil or ginseng seed cake extract according to the present invention is maintained for a certain period of time.
- Example 4 containing the ginseng flower extract of Example 1 and Example 5 containing the ginseng seed extract according to the present invention shows the effect of inhibiting excessive sebum secretion sebum control effect It was confirmed that there is. Therefore, it is possible to prevent acne and troubles caused by excessive sebum occurring on the skin including the scalp.
- Human fibroblasts were cultured in a 12-hole plate incubator at a concentration of 10 5 and then subjected to LPS treatment, followed by a medium containing 1 ppm, 10 ppm and 100 ppm of ginseng flower extract of Example 1, and the ginseng seed oil of Example 2 3 ginseng seed cake extract was replaced with a medium containing 1 ppm, 50 ppm.
- Cells were harvested on the second day of culture (harvest) and the amount of cyclooxygenase-2 (COX-2) produced was quantified using the Western blot method.
- a comparison with the value measured with a densitometer using the control group as 100 is shown in Table 13 below. At this time, the control group is an untreated group cultured without the ginseng flower extract of Example 1, ginseng seed oil of Example 2 and ginseng seed cake extract of Example 3.
- the ginseng flower extract of Example 1, the ginseng seed oil of Example 2 and the ginseng seed park extract of Example 3 according to the present invention is a cyclook induced by LPS which is an inflammatory response inducing factor It was found that suppressing the inflammatory response by reducing the biosynthesis of cagenase-2 concentration-dependently. In addition, it showed an effect that can alleviate skin problems.
- Participants in the experiment were 15 women with severe skin problems including acne. After washing the face with soap in a constant temperature and humidity room for 30 minutes, the skin trouble was measured by comparing the number of acne and the number with inflammation. .
- Example 14 The test subjects were allowed to use the nutrition creams of Example 4, Example 5, and Comparative Example 1 for 4 weeks, and then the degree of acne and skin troubles was compared, and the results are shown in Table 14 below.
- Example 5 containing the ginseng seed oil of Example 4, Example 2 and the ginseng seed cake extract of Example 3 including the ginseng flower extract of Example 1 according to the present invention
- the number of existing acne was reduced and the effects of inflammation accompanied were improved.
- LDPI Laser Doppler Perfusion Imager
- the initial values were measured using an IRPI (Infrad) camera, an LDPI and skin temperature measuring instrument.
- IRPI Infrad
- LDPI was used to measure the initial blood flow in the lower part of the forehead, and the IR temperature was measured for the forehead, under eye and cheek skin.
- Example 4 containing the ginseng flower extract according to the present invention and Example 5 containing the ginseng seed oil and ginseng seed park extract, the color of the blood due to the peripheral blood circulation improvement effect on the face of the test subjects It was confirmed that the improvement, which ultimately suggests that the ginseng flower extract or ginseng seed extract according to the present invention may effectively contribute to the skin nutrients, inhibit skin aging and delay.
- composition containing the ginseng flower extract prepared in Example 1, ginseng seed oil and ginseng seed cake extract prepared in Examples 2 and 3 were prepared by mixing according to the composition of Table 16 below.
- Example 6 Example 7
- Example 8 Comparative Example 2 Ginseng Flower Extract (Example 1) 10 - - - Ginseng Seed Oil (Example 2) - 10 - - Ginseng Seed Extract (Example 3) - - 0.1 - Caprylic Capric Triglyceride 70 70 70 80 ethanol 10 10 10 10 water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount
- Example 17 Treatment group Fallout parameter number (standard) / standard deviation Comparative Example 2 82.80 / 10.32
- Example 6 (Ginseng Flower Extract) 75.07 / 8.70
- Example 7 (Ginseng Seed Oil) 76.27 / 9.10
- Example 8 (Ginseng Seed Extract) 79.21 / 6.10
- Example 1 Green Flower Extract
- Example 2 Green Seed Oil
- Example 3 Green Seed Extract
- the experiment was performed using the broth dilution method. At this time, after performing double dilution, and incubated at 32 °C for 24 hours and 48 hours, it is determined whether the growth of the bacteria to the extent of suspension to the minimum concentration without MIC (Minimum Inhibition Concentration, minimum inhibition) Concentration). If the mixed solution is opaque and it is difficult to determine the growth of bacteria, it was confirmed by microscopic observation. The results are shown in Table 18 below.
- the hair samples were measured for gloss on a coordinator photometer equipped with a halogen lamp.
- the coherent photometer has an angle of incidence of light of 30 degrees and a reflected value of 0 to 90 degrees can be obtained.
- Glossiness was calculated by the following equation.
- hBM-MSCs Human bone marrow mesenchymal stem cells
- hBM-MSCs Human bone marrow mesenchymal stem cells
- Adipocyte differentiation was performed using a method recommended by Lonza, except that troglitazone (TRO) was used instead of indomethacin to induce adipocyte differentiation of mesenchymal stem cells.
- TRO troglitazone
- mesenchymal stem cells into adipocytes was carried out by incubating 1 g / ml of insulin, dexamethasone (DEXA) 1M, 0.5 mM isobutylmethylxanthine (IBX) and TRO 2M in the culture of mesenchymal stem cells. I was.
- DEXA dexamethasone
- IBX isobutylmethylxanthine
- the ginseng flower extract of Example 1 the ginseng seed oil of Example 2, the ginseng seed extract of Example 3, the troglitazone, and the glibenclamide (Sigma-aldrich, Each 10M was added to the medium after each 10M treatment (treated every two days for 10 days). After 10 days of induction of adipocyte differentiation of mesenchymal stem cells, the cell culture medium was removed, the cells were washed with phosphate buffered saline (PBS), and then the cells were washed with 0.2ml / cm 2 of 10% formalin / PBS solution. Was fixed for 10 minutes.
- PBS phosphate buffered saline
- the fixed cells were washed once with 0.5 ml / cm 2 of 60% isopropanol solution, and the lipid body in fat cells was stained with 0.2 ml / cm 2 for 10 minutes in a stain solution of Oil Red O in 60% isopropanol. Stained. The stained oil red o was eluted with 100% isopropanol solution and then absorbance was measured at a wavelength of 500 nm. The results are shown in Table 20 below. Each data value was corrected with an IDX (-) negative control.
- Table 21 Ingredient Content (% by weight) Purified water Remaining amount Ginseng Flower Extract or Ginseng Seed Extract 0.1 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Fiji-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity
- Table 22 Ingredient Content (% by weight) Purified water Remaining amount Ginseng Flower Extract or Ginseng Seed Extract 0.1 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Montana 202 (Manufacturer: Seppic) 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity
- Table 23 Ingredient Content (% by weight) Purified water Remaining amount Ginseng Flower Extract or Ginseng Seed Extract 0.1 Beeswax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Montana 202 (Manufacturer: Seppic) 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity
- Table 24 Ingredient Content (% by weight) Purified water Remaining amount Ginseng Flower Extract or Ginseng Seed Extract 0.1 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Fiji-12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, flavoring Quantity
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Abstract
Description
시험물질 | ROS 생성율(%) |
Non-UVB | 100 |
UVB | 161 |
UVB + 실시예 1(10 ppm) | 78 |
UVB + 실시예 1(50 ppm) | 72 |
UVB + 실시예 1(100 ppm) | 70 |
UVB + 비타민 C 100 uM | 72 |
시험물질 | ROS 생성 억제 비율(%) |
대조군 | 0 |
트롤록스 0.1 mM | 42.7 |
실시예 2(10 ppm) | 52.9 |
실시예 2(1 ppm) | 38.3 |
실시예 3(10 ppm) | 58.1 |
실시예 3(1 ppm) | 45.9 |
시험물질 | 처리 농도 | 테일 길이(㎛) | 저해율(%) |
대조군 | 처리없음 | 112.4 | - |
비타민 E | 10 ppm | 68.9 | 38.7 |
실시예 1 | 10 ppm | 104.6 | 6.9 |
50 ppm | 70.2 | 37.5 | |
100 ppm | 65.4 | 41.8 | |
실시예 2 | 1 ppm | 70.7 | 37.1 |
10 ppm | 39.1 | 43.2 | |
실시예 3 | 1 ppm | 76.5 | 26.9 |
10 ppm | 42.3 | 39.8 |
성 분 | 실시예 4 | 실시예 5 | 비교예 1 |
인삼 꽃 추출물 | 0.1 | - | - |
인삼 씨 오일 | - | 0.1 | - |
인삼씨박 추출물 | - | 0.1 | - |
밀납 | 10 | 10 | 10 |
폴리솔베이트60 | 1.5 | 1.5 | 1.5 |
피이지 60 경화피마자유 | 2 | 2 | 2 |
솔비탄세스퀴올레이트 | 0.5 | 0.5 | 0.5 |
유동파라핀 | 10 | 10 | 10 |
스쿠알란 | 5 | 5 | 5 |
카프릴릭/카프릭트리글리세라이드 (Estasan: Uniqema(제조사)) | 5 | 5 | 5 |
글리세린 | 5 | 5 | 5 |
부틸렌글리콜 | 3 | 3 | 3 |
프로필렌글리콜 | 3 | 3 | 3 |
트리에탄올아민 | 0.2 | 0.2 | 0.2 |
방부제, 색소, 향료 | 적량 | 적량 | 적량 |
정제수 | 잔량 | 잔량 | 잔량 |
멜라닌 생성 억제율(%) | 실시예 1 | 실시예 2 | 실시예 3 |
비처리군 | 0 | 0 | 0 |
10 ppm 처리군 | 4 | 3 | 23 |
50 ppm 처리군 | 25 | 19 | 32 |
100 ppm 처리군 | 49 | 43 | 51 |
알부틴 100 ppm | 51 |
시험물질 | 피부색 밝기 정도(△L*) |
실시예 1 | 1.91 ±0.17 |
실시예 2 | 1.94 ±0.11 |
실시예 3 | 1.91 ±0.14 |
하이드로 퀴논(양성 대조군) | 1.89 ±0.15 |
용매(Vehicle)(음성 대조군) | 0.51 ±0.13 |
시험 물질 | 각질형성세포에서의 분화능(%) | |
대조군 | 저칼슘처리군(Ca2+ 0.03 mM) | 100 |
고칼슘처리군(Ca2+ 1.2 mM) | 215 | |
실시예 1 | 1 ppm | 117 |
10 ppm | 122 | |
100 ppm | 125 | |
실시예 2 | 1 ppm | 111 |
10 ppm | 119 | |
100 ppm | 137 | |
실시예 3 | 1 ppm | 97 |
10 ppm | 109 | |
100 ppm | 121 |
시험물질 | 트랜스글루타미네이즈 발현량(%) | |
대조군 | 저칼슘처리군(Ca2+ 0.03 mM) | 100 |
고칼슘처리군(Ca2+ 1.2 mM) | 138 | |
실시예 1 | 1 ppm | 98 |
10 ppm | 119 | |
100 ppm | 141 |
시험물질 | 적용전 | 1주 | 2주 | 3주 | 4주 |
비교예 1 | 246 | 240 | 239 | 235 | 230 |
실시예 4 | 248 | 237 | 232 | 216 | 211 |
실시예 5 | 244 | 235 | 231 | 217 | 211 |
시험물질(농도:ppm) | COX-2 생합성(%) | |
실시예 1 | 100 | 56 |
10 | 64 | |
1 | 89 | |
실시예 2 | 50 | 42 |
1 | 78 | |
실시예 3 | 50 | 62 |
1 | 89 | |
대조군 | 100 |
성분 (중량%) | 실시예 6 | 실시예 7 | 실시예 8 | 비교예 2 |
인삼 꽃 추출물(실시예 1) | 10 | - | - | - |
인삼씨 오일 (실시예 2) | - | 10 | - | - |
인삼씨박 추출물 (실시예 3) | - | - | 0.1 | - |
카프릴릭카프릭트리글리세라이드 | 70 | 70 | 70 | 80 |
에탄올 | 10 | 10 | 10 | 10 |
물 | 잔량 | 잔량 | 잔량 | 잔량 |
처리군 | 탈락모개수평균(개)/표준편차 |
비교예 2 | 82.80 / 10.32 |
실시예 6 (인삼 꽃 추출물) | 75.07 / 8.70 |
실시예 7 (인삼씨 오일) | 76.27 / 9.10 |
실시예 8 (인삼씨박 추출물) | 79.21 / 6.10 |
처리군 | MIC(최소 억제 농도) |
실시예 1 (인삼 꽃 추출물) | 0.05% |
실시예 2 (인삼씨 오일) | 0.04% |
실시예 3 (인삼씨박 추출물) | 0.07% |
처리군 | d | Sf | G |
비교예 2 | 6.49 | 68.41 | 10.54 |
실시예 6 (인삼 꽃 추출물) | 3.58 | 83.20 | 23.24 |
실시예 7 (인삼씨 오일) | 2.81 | 87.45 | 31.12 |
실시예 8 (인삼씨박 추출물) | 3.91 | 64.23 | 16.43 |
처리군 | Oil Red 상대적 염색 비율 |
비교예 | 1.00 |
실시예 1 (인삼 꽃 추출물) | 2.18 |
실시예 2 (인삼씨 오일) | 2.74 |
실시예 3 (인삼씨박 추출물) | 1.75 |
배합성분 | 함량(중량%) |
정제수 | 잔량 |
인삼 꽃 추출물 또는 인삼씨 추출물 | 0.1 |
부틸렌글리콜 | 2.0 |
프로필렌글리콜 | 2.0 |
카르복시비닐폴리머 | 0.1 |
피이지-12 노닐페닐에테르 | 0.2 |
폴리솔베이트 80 | 0.4 |
에탄올 | 10.0 |
트리에탄올아민 | 0.1 |
방부제, 색소, 향료 | 적량 |
배합성분 | 함량(중량%) |
정제수 | 잔량 |
인삼 꽃 추출물 또는 인삼씨 추출물 | 0.1 |
밀납 | 4.0 |
폴리솔베이트 60 | 1.5 |
솔비탄세스퀴올레이트 | 1.5 |
유동파라핀 | 0.5 |
Montana 202 (제조사:Seppic) | 5.0 |
글리세린 | 3.0 |
부틸렌글리콜 | 3.0 |
프로필렌글리콜 | 3.0 |
카르복시비닐폴리머 | 0.1 |
트리에탄올아민 | 0.2 |
방부제, 색소, 향료 | 적량 |
배합성분 | 함량(중량%) |
정제수 | 잔량 |
인삼 꽃 추출물 또는 인삼씨 추출물 | 0.1 |
밀납 | 10.0 |
폴리솔베이트 60 | 1.5 |
피이지 60 경화피마자유 | 2.0 |
솔비탄세스퀴올레이트 | 0.8 |
유동파라핀 | 40.0 |
스쿠알란 | 5.0 |
Montana 202 (제조사:Seppic) | 4.0 |
글리세린 | 5.0 |
부틸렌글리콜 | 3.0 |
프로필렌글리콜 | 3.0 |
트리에탄올아민 | 0.2 |
방부제, 색소, 향료 | 적량 |
배합성분 | 함량(중량%) |
정제수 | 잔량 |
인삼 꽃 추출물 또는 인삼씨 추출물 | 0.1 |
폴리비닐알콜 | 13.0 |
소듐카르복시메틸셀룰로오스 | 0.2 |
글리세린 | 5.0 |
알란토인 | 0.1 |
에탄올 | 6.0 |
피이지-12 노닐페닐에테르 | 0.3 |
폴리솔베이트 60 | 0.3 |
방부제, 색소, 향료 | 적량 |
Claims (33)
- 인삼씨 추출물 및 인삼 꽃 추출물 중 하나 이상을 유효 성분으로 함유하는 피부 외용제 조성물.
- 제1항에 있어서, 상기 인삼씨 추출물은 인삼씨 오일인 것을 특징으로 하는 피부 외용제 조성물.
- 제2항에 있어서, 상기 인삼씨 추출물은 전체 피부 외용제 조성물 총 중량에 대하여 0.001 내지 100 중량%의 농도로 함유되어 있는 것을 특징으로 하는 피부 외용제 조성물.
- 제1항에 있어서, 상기 인삼씨 추출물은 인삼씨박 추출물인 것을 특징으로 하는 피부 외용제 조성물.
- 제4항에 있어서, 상기 인삼씨박 추출물은 전체 피부 외용제 조성물 총 중량에 대하여 0.001 내지 10 중량%의 농도로 함유되어 있는 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부 외용제 조성물은, 피부 손상을 억제하여 피부를 보호하는 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제6항에 있어서, 상기 피부 외용제 조성물은, 산화 또는 자외선에 의한 피부 손상을 억제하는 작용을 통해 피부를 보호하는 것을 특징으로 하는 피부 외용제 조성물.
- 제6항에 있어서, 상기 피부는 두피 또는 모발인 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부 외용제 조성물은, 피부 노화를 예방 또는 개선하는 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제9항에 있어서, 상기 피부 외용제 조성물은, 산화 또는 자외선에 의한 피부 손상 억제, 콜라겐 생성 촉진 또는 매트릭스 메탈로프로테이나아제 I(MMP-1)의 생합성 저해를 통해 피부의 노화를 억제하는 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부 외용제 조성물은, 피부 주름을 예방 또는 개선시키는 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제11항에 있어서, 상기 피부 외용제 조성물은, 콜라겐 생성 촉진 또는 매트릭스 메탈로프로테이나아제 I(MMP-1)의 생합성 저해를 통해 피부 주름을 예방 또는 개선시키는 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부 외용제 조성물은, 피부 탄력을 개선시키는 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부 외용제 조성물은, 피부 미백 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제14항에 있어서, 상기 피부 외용제 조성물은, 멜라닌 생성 억제 또는 피부에 침착된 색소의 미백화를 통해 피부 미백을 수행하는 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부 외용제 조성물은, 피부 보습 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제16항에 있어서, 상기 피부 외용제 조성물은, 각질 형성 세포의 분화 촉진 또는 트랜스 글루타미네이즈 발현 촉진을 통해 피부 수분을 유지하는 것을 특징으로 하는 피부 외용제 조성물.
- 제16항에 있어서, 상기 피부 외용제 조성물은, 아토피 또는 건선을 포함하는 피부 건조 질환을 예방 또는 개선하는 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부 외용제 조성물은, 피부 각질 제거의 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부 외용제 조성물은, 피부의 피지 조절 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제20항에 있어서, 상기 피부 외용제 조성물은, 피지의 분비를 억제함으로써 피부의 피지를 조절하는 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부 외용제 조성물은 염증의 예방 또는 개선, 또는 자극 완화의 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제22항에 있어서, 상기 피부는 두피인 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부 외용제 조성물은, 여드름을 포함하는 피부 트러블을 예방 또는 개선하는 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제24항에 있어서, 상기 피부는 두피인 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부 외용제 조성물은, 피부 혈색을 개선하는 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부는 두피 또는 모발인 것을 특징으로 하는 피부 외용제 조성물.
- 제27항에 있어서, 상기 피부 외용제 조성물은, 탈모 예방의 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제27항에 있어서, 상기 피부 외용제 조성물은, 항비듬 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제27항에 있어서, 상기 피부 외용제 조성물은, 모발의 광택 또는 부드러움 증진 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부 외용제 조성물은, 얼굴 또는 바디의 볼륨 및 탄력 증진 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
- 제31항에 있어서, 상기 피부 외용제 조성물은, 지방세포 분화 촉진을 통해 얼굴 또는 바디의 볼륨 및 탄력을 증진시키는 것을 특징으로 하는 피부 외용제 조성물.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 피부 외용제 조성물은, 얼굴 또는 바디 라인 개선의 용도를 갖는 것을 특징으로 하는 피부 외용제 조성물.
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JP2011513407A JP5628797B2 (ja) | 2008-06-13 | 2009-04-28 | 人参の花または人参の種子抽出物を含有する皮膚外用剤組成物 |
CN2009801221901A CN102065836A (zh) | 2008-06-13 | 2009-04-28 | 含有人参的花或人参的种子提取物的皮肤外用剂组合物 |
US12/997,822 US8709511B2 (en) | 2008-06-13 | 2009-04-28 | External preparation composition for skin comprising ginseng flower or ginseng seed extracts |
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KR1020080124500A KR20090129928A (ko) | 2008-06-13 | 2008-12-09 | 인삼 꽃 추출물을 함유하는 피부 외용제 조성물 |
KR1020080124479A KR101550917B1 (ko) | 2008-06-16 | 2008-12-09 | 인삼씨 추출물을 함유하는 피부 외용제 조성물 |
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KR101877803B1 (ko) * | 2018-04-16 | 2018-07-13 | (주)아모레퍼시픽 | 플로랄진세노사이드를 함유하는 피부 외용제 조성물 |
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