WO2014162878A1 - 自動分析装置及び分析方法 - Google Patents
自動分析装置及び分析方法 Download PDFInfo
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- WO2014162878A1 WO2014162878A1 PCT/JP2014/057601 JP2014057601W WO2014162878A1 WO 2014162878 A1 WO2014162878 A1 WO 2014162878A1 JP 2014057601 W JP2014057601 W JP 2014057601W WO 2014162878 A1 WO2014162878 A1 WO 2014162878A1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00594—Quality control, including calibration or testing of components of the analyser
- G01N35/00693—Calibration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
- G01N21/272—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/0092—Scheduling
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00594—Quality control, including calibration or testing of components of the analyser
- G01N35/00693—Calibration
- G01N2035/00702—Curve-fitting; Parameter matching; Calibration constants
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N2035/0097—Control arrangements for automatic analysers monitoring reactions as a function of time
Definitions
- the present invention relates to an automatic analyzer that performs qualitative / quantitative analysis of biological samples such as blood and urine, and more particularly to an automatic analyzer and analysis method involved in coagulation and hemostasis.
- Blood coagulation tests are performed for the purpose of understanding the pathology of the coagulation and fibrinolytic system, diagnosing DIC (disseminated intravascular coagulation syndrome), confirming the effect of thrombus treatment, and diagnosing hemophilia.
- blood coagulation time measurement is a test that directly checks whether the coagulation reaction that generates fibrin is working correctly. If there is a congenital or acquired abnormality, the blood coagulation time is prolonged.
- the blood coagulation test is important as a means of screening for bleeding tendency caused by various causes such as abnormalities such as blood coagulation factors and von Willebrand factors, liver damage, vitamin K deficiency, inhibitor production, and DIC.
- Electric resistance detection method, optical detection method, mechanical method, etc. are mainly used for detection of fibrin precipitation by automatic analyzer, and mainstream is optical detection method (transmitted light detection, scattering) with excellent processing ability.
- Light detection and mechanical method (viscosity detection).
- the parameter of the approximate expression is calculated using the measurement value that changes with the passage of time, and according to the degree of convergence of the parameter,
- a method is known in which the degree of convergence of a reaction is determined and a measured value at the end of the reaction is calculated using parameters at the time when the reaction is determined to have converged (see, for example, Patent Document 1).
- fibrin deposition In detection of fibrin deposition in blood coagulation tests, there are two pathways leading to fibrin deposition: an intrinsic pathway and an extrinsic pathway, and may exhibit unstable behavior when the reaction pathway is abnormal.
- the fibrin precipitation step is a step of synthesizing fibrin monomer from fibrinogen, and electrostatic binding of fibrin monomer It consists of a multi-step reaction, such as a step of starting the production of fibrin polymer.
- APTT Activated Partial Thromboplastin Time
- An object of the present invention is to provide an automatic analyzer and an analysis method capable of accurately calculating the coagulation time.
- the present invention provides a detection unit for measuring a coagulation index indicating the amount of blood coagulated in a sample of blood dispensed with a reagent, and curve fitting based on the measured coagulation index.
- a detection unit for measuring a coagulation index indicating the amount of blood coagulated in a sample of blood dispensed with a reagent, and curve fitting based on the measured coagulation index.
- An initial coagulation index change determination unit that determines whether or not there is an initial coagulation index change indicating a portion where the coagulation index increases immediately after the reagent is dispensed into the sample, and based on the presence or absence of the initial coagulation index change,
- a coagulation time calculation unit for calculating time.
- the coagulation time can be accurately calculated.
- FIG. 1 is a configuration diagram of an automatic analyzer 100 according to an embodiment of the present invention.
- the automatic analyzer 100 mainly includes a sample dispensing probe 101, a sample disk 102, a reagent dispensing probe 106, a reagent disk 107, a reaction container stock unit 111, a reaction container transport mechanism 112, a detection unit 113, a reaction container discarding unit 117, An operation computer 118 and a control computer 120 are provided.
- the control computer includes an analysis operation control unit 120A that controls an analysis operation and a data processing unit 120B that performs data processing.
- the analysis operation control unit 120A of the control computer controls a series of operations from sample and reagent dispensing to reaction container transfer and reaction container disposal.
- the sample dispensing probe 101 sucks the sample in the sample container 103 arranged on the sample disk 102 that rotates clockwise and counterclockwise, and discharges it to the reaction container 104. Specifically, the sample dispensing probe 101 performs a sample suction operation and a discharge operation in accordance with the operation of the sample syringe pump 105.
- the reagent dispensing probe 106 sucks the reagent in the reagent container 108 arranged on the reagent disk 107 and discharges it to the reaction container 104. Specifically, the reagent dispensing probe 106 performs a reagent suction operation and a discharge operation in accordance with the operation of the reagent syringe pump 110.
- a reagent heating mechanism 109 is built in the reagent dispensing probe 106.
- the reagent sucked by the reagent dispensing probe 106 is heated to an appropriate temperature (predetermined temperature) by the reagent heating mechanism 109.
- Reaction container transport mechanism 112 transports and installs reaction container 104. Specifically, the reaction container transport mechanism 112 moves and installs the reaction container 104 from the reaction container stock unit 111 to the reaction container installation unit 114 of the detection unit 113 by holding and rotating the reaction container 104.
- the reaction vessel installation section 114 is provided with a recess so that the reaction vessel 104 can be placed, and the reaction vessel 104 can be inserted into this recess.
- the apparatus includes at least one detection unit 113.
- a blood coagulation reaction is started. That is, the blood coagulation reaction starts with the reagent discharge operation by the reagent dispensing probe 106 as a starting point.
- Analysis items to be analyzed for each sample are input from the operation computer 118 to the control computer 120 via the operation screen displayed on the keyboard KB or the display device D.
- the analysis item may be input by clicking the analysis item displayed on the display device D with the mouse M.
- the operation of the detection unit 113 is controlled by the control computer 120.
- the light source 115 irradiates the reaction vessel 104 with light.
- the light emitted from the light source 115 is scattered by the reaction solution in the reaction vessel 104.
- the detection unit (light sensor) 116 is configured by a photodiode or the like.
- the detection unit 116 receives scattered light scattered by the reaction solution in the reaction vessel 104 and performs light / current conversion. Thereby, the detection part 116 outputs the photometry signal which shows the intensity
- the photometric signal is A / D converted by the A / D converter 121 and input to the control computer 120 via the interface 122.
- the data processing unit 120B in the control computer calculates the coagulation time based on the photometric signal.
- the data processing unit 120B outputs the calculated coagulation time to the display device D connected to the operation computer 118 and stores it in the hard disk 119 as a storage device. Note that the data processing unit 120B may print out the coagulation time as a calculation result to the printer 123 via the interface 122.
- the reaction container transport mechanism 112 holds the reaction container 104 for which photometry has been completed and discards it to the reaction container discarding unit 117.
- FIG. 2 is a schematic diagram showing an ideal coagulation reaction curve when the analysis item in the automatic analyzer 100 according to the embodiment of the present invention is APTT.
- the vertical axis represents the scattered light intensity E
- the horizontal axis represents time t.
- the scattering intensity E becomes a certain maximum value E P.
- a region where the scattered light intensity E becomes constant minimum value E b is called the baseline region, a region where the scattered light intensity E becomes a constant maximum value E P of the plateau region.
- Scattered light variation E range is indicative of a difference between the maximum value E P and the minimum value E b of the scattered light intensity.
- the control computer 120 determines an approximate curve representing a coagulation reaction curve by curve fitting the measurement data of the scattered light intensity E. Details of the operation of the control computer 120 will be described later with reference to FIG.
- curve fitting is defined as finding the curve that best fits the measured data.
- regression analysis is used for curve fitting.
- curve fitting a predetermined iterative process is performed to determine a parameter for a function having one or more parameters.
- the curve does not necessarily pass through a data point (a point representing a measured value).
- a predetermined iterative calculation process is performed to determine a parameter that minimizes the distance between the curve and the data point group, and an optimum parameter is searched and determined.
- a curve obtained by curve fitting is defined as an approximate curve
- a parameter determined by curve fitting is defined as an approximate parameter
- an expression obtained by substituting the approximate parameter into the original function is defined as an approximate expression
- a sigmoid curve represented by the following equation (1) can be adopted as an approximate function.
- parameter a represents time t at the inflection point P A shown in FIG. 2
- the parameter k is the degree of slope of the rising portion of the slope (coagulation reaction curve of the curve in a region sandwiched between the baseline region and the plateau region ).
- FIG. 3 is a diagram showing an example of an actual coagulation reaction curve when the analysis item is APTT in the automatic analyzer 100 according to the embodiment of the present invention.
- the vertical axis indicates the scattered light intensity E
- the horizontal axis indicates time t.
- the coagulation reaction curve shown in FIG. 3 is abnormal in the reaction path and shows a two-stage reaction.
- the initial change in light amount D 1 are observed immediately after the start of measurement as a response first step, then, is the original light intensity change D 2 seen as a reaction of the two-stage.
- the initial light amount change indicates a portion where the scattered light intensity E increases immediately after the reagent is dispensed into the sample in the coagulation reaction curve. That is, in the initial light quantity change, the time derivative of the scattered light intensity E is positive (dE / dt> 0).
- approximate parameters E range ⁇ 0 and k ⁇ 0.
- the original light amount change indicates a portion where the scattered light intensity E increases after the initial light amount change in the coagulation reaction curve. That is, with the original change in light quantity, the time derivative of the scattered light intensity E is positive (dE / dt> 0). In Figure 3, the time derivative of the scattered light intensity E in the original light amount change D 2 is greater than the time derivative of the scattered light intensity E in the initial light amount change D 1.
- FIG. 4 illustrating the transition of the approximation parameters in the initial light amount change D 1 shown in FIG. Figure 4 is a diagram of assistance in explaining the transition of the approximation parameters in the initial light amount change D 1 shown in FIG.
- FIG. 4A is a diagram illustrating a result of calculating and plotting the approximate parameter E range for the initial light quantity change D 1 illustrated in FIG. 3 every 5 seconds.
- the vertical axis represents the approximate parameter E range and the horizontal axis represents time t.
- the time range is 0 ⁇ t ⁇ 35.
- E range becomes a substantially constant value in the vicinity of 15 to 30 seconds.
- FIG. 4 (B) the approximate parameters k in the initial light amount change D 1 shown in FIG. 3 is calculated every five seconds, it shows the results of plotting.
- the vertical axis indicates the approximate parameter k
- the horizontal axis indicates time t.
- the time range is 0 ⁇ t ⁇ 35.
- k becomes a substantially constant value in the vicinity of 15 to 30 seconds.
- FIG. 5 illustrating the transition of the approximation parameters in the original light amount change D 2 or later shown in FIG.
- Figure 5 is a diagram of assistance in explaining the transition of the approximation parameters in the original light amount change D 2 or later shown in FIG.
- FIG. 5 (A) and calculates an approximate parameter a in the original light amount change D 2 or later shown in FIG. 3 every 5 seconds, it shows the results of plotting.
- the vertical axis indicates the approximate parameter a
- the horizontal axis indicates time t.
- the time range is t ⁇ 35.
- Inflection point P A shown in FIG. 2 there shall be present in the middle of the sigmoid curve, firstly, the approximate parameter a indicating the value of the time t in the inflection point P A converges.
- the approximate parameter a converges to a constant value after about 40 seconds from the start of measurement.
- FIG. 5 (B) shows the results of plotting.
- the vertical axis indicates the approximate parameter k
- the horizontal axis indicates time t.
- the time range is t ⁇ 35.
- the approximate parameter k converges.
- the approximate parameter k converges to a constant value after about 50 seconds.
- FIG. 6 is a block diagram showing functions of the control computer 120 used in the automatic analyzer 100 according to the embodiment of the present invention.
- the data processing unit 120B in the control computer includes a first curve fitting unit 120B-a, an initial coagulation index change determining unit 120B-b, a coagulation reaction completion determining unit 120B-c, a second curve fitting unit 120B-d, and parameters.
- a convergence determining unit 120B-e, a coagulation time calculating unit 120B-f, a reaction start point determining unit 120B-g, and a third curve fitting unit 120B-h are provided.
- the first curve fitting 120B-a performs curve fitting on the measurement data and calculates approximate parameters.
- the initial coagulation index change determination unit 120B-b determines whether or not there is an initial light amount change based on the calculated approximate parameter.
- the coagulation reaction completion determination unit 120B-c determines whether or not the first-stage reaction is completed in the two-stage coagulation reaction when there is an initial light amount change.
- the second curve fitting unit 120B-d performs curve fitting on the measurement data of only the second-stage reaction excluding the first-stage reaction, and sets approximate parameters. calculate.
- the parameter convergence determination unit 120B-e determines whether or not the approximate parameter has converged.
- the coagulation time calculation unit 120B-f calculates the coagulation time based on the approximate function into which the approximate parameters are substituted.
- the reaction start point determination unit 120B-g determines the reaction start point at which the optical change starts from the measurement data when there is no change in the initial light amount.
- the third curve fitting unit 120B-h performs curve fitting from the reaction start point to the measurement end point when there is no change in the initial light amount, and calculates an approximate parameter.
- FIG. 7 is a flowchart showing the operation of the automatic analyzer 100 according to the embodiment of the present invention.
- the analysis item is APTT and the coagulation reaction is a two-step reaction will be described as an example.
- the analysis item is not limited to APTT.
- the detection unit 113 starts from the time when dispensing of the reagent and sample into the reaction container 104 placed on the reaction container installation unit 114 is completed (step S10; Yes), and calculates the amount of scattered light from the reaction container 104.
- the scattered light intensity shown is continuously detected (measured) (step S20).
- the A / D converter 121 collects a photometric signal (light / current conversion data) indicating the intensity of scattered light detected by the detection unit 113 at regular intervals (S seconds), and performs A / D conversion.
- the first curve fitting unit 120B-a receives A / D conversion data (A / D converted photometric signal) from the A / D converter 121 (step S30).
- the first curve fitting unit 120B-a performs curve fitting with respect to the A / D conversion data at a constant period, and calculates parameters of the approximate curve (step S40).
- the curve fitting cycle the higher the resolution, so that the end of measurement can be determined.
- the curve fitting period may not match the conversion period S seconds in the A / D converter.
- the curve fitting cycle may be changed for each analysis item, or the same cycle may be used for all or part of the analysis items.
- a part of the continuously collected measurement data may be thinned out. That is, even if measurement is performed at intervals of 0.1 seconds, the number of data can be reduced to 1/10 by thinning out the data at intervals of 1 second at the time of curve fitting, and the load on the control computer 120 can be reduced.
- the first curve fitting unit 120B-a is an approximate parameter calculated by curve fitting, E range (1) -1 , a (1) -1 , b (1) -1 , k (1) -1 and E b (1) -1 are stored in the hard disk 119 (step S50). At this time, the first curve fitting unit 120B-a stores the value of each parameter every time the approximate parameter is calculated. As a result, the first curve fitting unit 120B-a stores the change over time for each approximate parameter, and monitors the approximate parameter that changes with time.
- the initial coagulation index change determination unit 120B-b confirms whether or not there is an initial light quantity change, and appropriately selects (changes) a process for calculating the coagulation time according to the presence or absence of the initial light quantity change. This is one of the crucial differences between the present invention and the conventional example.
- the initial coagulation index change determination unit 120B-b confirms the presence or absence of an initial light quantity change by monitoring the approximate parameters Erange and k (step S60). Specifically, the initial coagulation index change determination unit 120B-b determines that there is an initial light amount change when E range ⁇ 0 or k ⁇ 0.
- the reaction will be in two stages, so it is necessary to carefully judge the reaction completion point so that the measurement is not accidentally terminated at the end of the first stage reaction. Therefore, it is necessary to determine whether or not there is a change in the initial light quantity. If there is a change in the initial light quantity, it is first necessary to confirm the completion of the first stage reaction.
- the coagulation reaction completion determination unit 120B-c determines whether or not the first-stage reaction has been completed by monitoring Erange and k (step S70).
- the coagulation reaction completion determination unit 120B-c determines that the first-stage reaction has been completed when Erange and k become constant.
- the end point of the period during which E range and k are constant is defined as the completion of the first stage reaction.
- E range is constant from 15 to 30 seconds
- the second curve fitting unit 120B-d receives A / D conversion data from the A / D converter 121 (step S80).
- the second curve fitting unit 120B-d performs curve fitting with respect to the A / D conversion data at a constant cycle by using the equation (2), and calculates parameters of the approximate curve (step S90).
- the second curve fitting unit 120B-d is an approximate parameter calculated by curve fitting, E range (1) -2 , a (1) -2 , b (1) -2 , k (1) -2 , E b (1) -2 are stored in the hard disk 119 (step S100).
- the fitting target data at this time is measured data of only the second-stage reaction excluding the first-stage reaction.
- the parameter convergence determination unit 120B-e ends the measurement with the convergence of the approximate parameters a and k.
- the parameter convergence determination unit 120B-e determines whether or not the approximate parameter a (1) -2 has converged and becomes constant (step S110).
- the parameter convergence determination unit 120B-e determines whether or not the approximate parameter k (1) -2 has converged and becomes constant when the approximate parameter a (1) -2 becomes constant (step S110; Yes). (Step S120).
- the parameter convergence determination unit 120B-e determines that both the approximate parameters a (1) -2 and k (1) -2 have converged (step S110; Yes, step S120; Yes)
- the parameter convergence determination unit 120B- The analysis operation control unit 120A of the control computer that has received the result of e instructs the end of the detection of the scattered light amount of the sample whose measurement is to be ended (step S130).
- the coagulation time calculation unit 120B-f uses the approximate parameters E range (1) -2 , a (1) -2 , b (1) -2 , k (1) for the second-stage peak stored when the measurement is completed. ⁇ 2 , E b (1) ⁇ 2 is substituted into equation (2) to calculate the coagulation time (step S140).
- the primary differential method is a method in which the time during which the differential value of the scattered light amount (change in the scattered light amount) is maximized is the coagulation time.
- the second derivative method is a method in which the time during which the second derivative value of the amount of scattered light is maximized is used as the coagulation time, and E range (1) -2 , a (1) -2 , b (1) -2 , k ( 1) -2 , E b (1) -2
- the maximum value when the approximate expression obtained by substituting (1) -2 into the expression (2) is second-order differentiated is calculated.
- a (1) -2 is used as it is for the solidification time.
- the data processing unit 120B of the control computer 120 transmits the calculated coagulation time to the operation computer 118 (step S150), and displays the result on the display device D (step S160).
- the two-stage reaction is an abnormal reaction
- an error is displayed so that the coagulation time can be calculated from the second-stage reaction.
- the coagulation reaction is a two-stage reaction, it is possible to calculate the coagulation time and inform the user of the abnormality.
- Modification 1 As another method for determining whether or not there is a change in the initial light amount, there is a method in which the measurement data is used as it is and the change in the initial light amount is present when the scattered light change threshold is exceeded.
- the data processing unit 120b of the control computer 120 performs an initial change. What is necessary is just to judge that there is quantity.
- Modification 2 In addition, immediately after the start of measurement, the presence or absence of the change in the initial light quantity is not judged, and the approximate parameter is continuously monitored until the preset minimum measurement time, and an outlier occurs after the temporary convergence of the approximate parameter. There is also a method for determining whether or not there is a change in the initial light quantity after confirming the above.
- a minimum measurement time may be set in advance for each analysis item.
- FIG. 8 is a diagram for explaining the transition of the approximate parameter in the present modification. For example, if the minimum measurement time is set to 50 seconds, from FIGS. 8 (A) and (B), a and k are almost constant values in the vicinity of 15 to 30 seconds, and the completion of the reaction is suspected. Continue measurement.
- FIG. 9 is a diagram showing another example of an actual coagulation reaction curve when the analysis item is APTT in the automatic analyzer 100 according to the embodiment of the present invention.
- the vertical axis represents scattered light intensity E
- the horizontal axis represents time t.
- the inflection point shown by P A showing a measurement completion point indicating a point at which the measurement is completed in P B.
- FIG. 10 is a diagram for explaining the transition of the approximate parameter in the coagulation reaction curve shown in FIG.
- FIG. 10 (A) is a diagram showing a result of calculating and plotting parameter a in the coagulation reaction curve shown in FIG. 9 every 5 seconds.
- the vertical axis indicates the approximate parameter a
- the horizontal axis indicates time t.
- FIG. 10 (B) is a diagram showing the result of calculating and plotting the parameter k in the coagulation reaction curve shown in FIG. 9 every 5 seconds.
- the vertical axis indicates the approximate parameter k
- the horizontal axis indicates time t.
- the approximate parameter k converges to a constant value after P B shown in FIG. 9 (after about 55 seconds from the start of measurement).
- the parameter convergence determination unit 120B-e determines whether or not the approximate parameter a (1) -1 has converged and becomes constant (step S170).
- step S170 When the approximate parameter a (1) -1 becomes constant (step S170; Yes), the parameter convergence determination unit 120B-e determines whether the approximate parameter k (1) -1 has converged and becomes constant. (Step S180).
- the analysis operation control unit 120A of the control computer reflects the result of the parameter convergence determination unit 120B-e, and when the amount of change of the approximate parameter k (1) -1 falls within a certain range, that is, the approximate parameter k ( 1) When ⁇ 1 becomes constant (step S180; Yes), the measurement of the sample that should be terminated is terminated, and the termination of the detection of the scattered light amount is instructed (step S190).
- the second decisive difference between the present invention and the conventional method is that the approximate parameter determined when the measurement is completed is not used as it is.
- the reaction start point determination unit 120B-g determines the reaction start point
- the third curve fitting unit 120B-h performs curve fitting.
- the reaction start point is a point where an optical change starts.
- the parameters E range (1) -2 , a (1) -2 in the equation (2) , B (1) -2 , k (1) -2 , and E b (1) -2 can be used as differential values in an approximate expression. That is, a region where there is no change in the amount of scattered light can be defined as before the reaction start point and after the reaction end.
- examples of the extension of the method for determining the reaction start point and end point include when the secondary differential value of the scattered light amount becomes zero and when the secondary differential value of the integrated value of the scattered light amount becomes zero.
- the third curve fitting unit 120B-h executes curve fitting from immediately before the reaction start point to the measurement end point (step S210), and approximate parameters E range (2) , a (2 ) , B (2) , k (2) , E b (2) are determined and stored in the hard disk 119 (step S220).
- the coagulation time calculation unit 120B-f substitutes the determined parameter into the equation (2) to calculate the coagulation time (step S230).
- the process in step S230 is the same as the process in step S140.
- the coagulation time calculation unit 120B-f transmits the calculated coagulation time to the operation computer 118 (step S240) and displays the result on the display device D (step S250).
- the present invention is not limited to the above-described embodiments, and includes various modifications.
- the above-described embodiments have been described in detail for easy understanding of the present invention, and are not necessarily limited to those having all the configurations described.
- a part of the configuration of one embodiment can be replaced with the configuration of another embodiment, and the configuration of another embodiment can be added to the configuration of one embodiment. It is possible to add, delete, and replace other configurations for a part of the configuration of each embodiment.
- the presence or absence of the initial light amount change is determined from the measurement data of the scattered light intensity E, but the measurement data may be any coagulation index indicating the amount of coagulated blood.
- the calculation process of the coagulation time is performed by the control computer 120.
- the A / D conversion data received by the control computer 120 is transmitted to the operation computer 118 via the interface, and the operation computer 118 is transmitted. You may process by.
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Abstract
Description
この際、第1のカーブフィッティング部120B-aは、近似パラメータが算出される度に個々のパラメータの値を記憶する。これにより、第1のカーブフィッティング部120B-aは、近似パラメータ毎の経時変化を記憶し、時間とともに変化する近似パラメータを監視する。
ここで、この時のフィッティングの対象データは、一段階目の反応を除いた二段階目の反応のみの測定データとする。
パーセント法とは、凝固時間算出部120B-fが、反応の開始から終了までの散乱光量変化のうち、一定の割合を閾値として、閾値を越える時間を凝固時間とする方法である。例えば、閾値が散乱光量変化の50%の場合には、Erange (1)-2× 0.5 +Eb=式(2)の右辺という方程式を解くことにより、凝固時間tを算出する。
一次微分法とは散乱光量の微分値(散乱光量変化)の最も大きくなる時間を凝固時間とする方法である。Erange (1)-2,a(1)-2,b(1)-2,k(1)-2,Eb (1)-2を式(2)に代入して得られる近似式を一次微分し、極大値を算出する。
二次微分法は散乱光量の二次微分値の最も大きくなる時間を凝固時間とする方法であり、Erange (1)-2,a(1)-2,b(1)-2,k(1)-2,Eb (1)-2を式(2)に代入して得られる近似式を二次微分した時の極大値を算出する。変極点法ではa(1)-2をそのまま凝固時間とする方法である。
初期光量変化の有無を判断する別の方法としては測定データをそのまま用いて、散乱光の変化の閾値を越えた場合には初期光量変化ありとする方法もある。
また、測定開始直後には初期光量変化の有無の判断を実施せず、あらかじめ設定した最低測定時間までは近似パラメータを監視続けるようにし、近似パラメータの一時的な収束の後に外れ値が発生するのを確認してから初期光量変化の有無を判断する方法もある。
さらに反応開始点と終了点の決定方法の拡張例としては、散乱光量の二次微分値がゼロになった時や散乱光量の積分値の二次微分値が0になった時などがある。
凝固時間算出部120B-fは、決定されたパラメータを式(2)に代入し、凝固時間を算出する(ステップS230)。なお、ステップS230の処理は、ステップS140の処理と同じである。
101…サンプル分注プローブ
102…サンプルディスク
103…サンプル容器
104…反応容器
105…サンプル用シリンジポンプ
106…試薬分注プローブ
107…試薬ディスク
108…試薬容器
109…試薬昇温機構
110…試薬用シリンジポンプ
111…反応容器ストック部
112…反応容器搬送機構
113…検出ユニット
114…反応容器設置部
115…光源
116…検出部(光センサ)
117…反応容器廃棄部
118…操作用コンピュータ
119…ハードディスク
120…制御用コンピュータ
121…A/D変換器
122…インターフェイス
123…プリンタ
Claims (6)
- 試薬が分注された血液のサンプルにおいて凝固した血液の量を示す凝固指数を測定する検出ユニットと、
測定された前記凝固指数に基づいて、カーブフィッティングにより凝固反応曲線の近似曲線のパラメータを所定の時間間隔で算出する第1のカーブフィッティング部と、
前記第1のカーブフィッティング部によって所定の時間間隔で算出された前記近似曲線のパラメータに基づいて、前記試薬が前記サンプルに分注された直後から前記凝固指数が増加する部分を示す初期凝固指数変化の有無を判断する初期凝固指数変化判断部と、
前記初期凝固指数変化の有無に基づいて、凝固時間を算出する凝固時間算出部と、
を備えることを特徴とする自動分析装置。 - 請求項1に記載の自動分析装置であって、
初期凝固指数変化があると判断された場合、一段階目の凝固反応が完了したか否かを判断する凝固反応完了判断部と、
一段階目の凝固反応が完了したと判断された場合、検出された前記凝固指数のうち、前記第1のカーブフィッティング部によってカーブフィッティングの対象にならなかった前記凝固指数に基づいて、カーブフィッティングにより凝固反応曲線の近似曲線のパラメータを所定の時間間隔で算出する第2のカーブフィッティング部と、
所定の時間間隔で算出された前記近似曲線のパラメータが一定の値に収束したか否かを判断するパラメータ収束判断部と、
を備え、
前記凝固時間算出部は、
初期凝固指数変化があると判断され、かつ、前記第2のカーブフィッティング部によって所定の時間間隔で算出された前記近似曲線のパラメータが一定の値に収束した場合、前記第2のカーブフィッティング部によって算出されたパラメータによって定まる近似曲線を表す近似関数に基づいて、凝固時間を算出する
ことを特徴とする自動分析装置。 - 請求項1に記載の自動分析装置であって、
所定の時間間隔で算出された前記近似曲線のパラメータが一定の値に収束したか否かを判断するパラメータ収束判断部と、
前記第1のカーブフィッティング部によって所定の時間間隔で算出された前記近似曲線のパラメータが一定の値に収束した場合、収束した前記近似曲線のパラメータに基づいて、前記凝固反応曲線において凝固反応が開始した点を示す反応開始点を決定する反応開始点決定部と、
前記反応開始点から、測定を終了した点を示す測定終了点までの間で、カーブフィッティングにより前記凝固指数から凝固反応曲線の近似曲線のパラメータを算出する第3のカーブフィッティング部と、
を備え、
前記凝固時間算出部は、
初期凝固指数変化がないと判断され、かつ、前記第1のカーブフィッティング部によって所定の時間間隔で算出された前記近似曲線のパラメータが一定の値に収束した場合、前記第3のカーブフィッティング部によって算出されたパラメータによって定まる近似曲線を表す近似関数に基づいて、凝固時間を算出する
を備えることを特徴とする自動分析装置。 - 請求項2に記載の自動分析装置であって、
前記第1のカーブフィッティング部によって所定の時間間隔で算出された前記近似曲線のパラメータが一定の値に収束した場合、収束した前記近似曲線のパラメータに基づいて、前記凝固反応曲線において凝固反応が開始した点を示す反応開始点を決定する反応開始点決定部と、
前記反応開始点から、測定を終了した点を示す測定終了点までの間で、カーブフィッティングにより前記凝固指数から凝固反応曲線の近似曲線のパラメータを算出する第3のカーブフィッティング部と、
を備え、
前記凝固時間算出部は、
初期凝固指数変化があると判断され、かつ、前記第2のカーブフィッティング部によって所定の時間間隔で算出された前記近似曲線のパラメータが一定の値に収束した場合、前記第2のカーブフィッティング部によって算出されたパラメータによって定まる近似曲線を表す近似関数に基づいて、凝固時間を算出し、
初期凝固指数変化がないと判断され、かつ、前記第1のカーブフィッティング部によって所定の時間間隔で算出された前記近似曲線のパラメータが一定の値に収束した場合、前記第3のカーブフィッティング部によって算出されたパラメータによって定まる近似曲線を表す近似関数に基づいて、凝固時間を算出する
を備えることを特徴とする自動分析装置。 - 請求項1に記載の自動分析装置であって、
前記近似曲線を表す近似関数は、
前記初期凝固指数変化判断部は、
前記第1のカーブフィッティング部によって所定の時間間隔で算出された前記近似曲線のパラメータErange及びkが共に0でない場合、前記初期凝固指数変化があると判断する
ことを特徴とする自動分析装置。 - 試薬が分注された血液のサンプルにおいて凝固した血液の量を示す凝固指数を測定する検出工程と、
測定された前記凝固指数に基づいて、カーブフィッティングにより凝固反応曲線の近似曲線のパラメータを所定の時間間隔で算出する第1のカーブフィッティング工程と、
前記第1のカーブフィッティング工程において所定の時間間隔で算出された前記近似曲線のパラメータに基づいて、前記試薬が前記サンプルに分注された直後から前記凝固指数が増加する部分を示す初期凝固指数変化の有無を判断する初期凝固指数変化判断工程と、
前記初期凝固指数変化の有無に基づいて、凝固時間を算出する凝固時間算出工程と、
を有することを特徴とする分析方法。
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