WO2009066695A1 - 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット - Google Patents

糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット Download PDF

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Publication number
WO2009066695A1
WO2009066695A1 PCT/JP2008/071023 JP2008071023W WO2009066695A1 WO 2009066695 A1 WO2009066695 A1 WO 2009066695A1 JP 2008071023 W JP2008071023 W JP 2008071023W WO 2009066695 A1 WO2009066695 A1 WO 2009066695A1
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WO
WIPO (PCT)
Prior art keywords
fecal sample
nucleic acids
preparing
solution
feces
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PCT/JP2008/071023
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English (en)
French (fr)
Inventor
Yasuo Tanigami
Tomonori Nagaoka
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Olympus Corporation
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Publication date
Application filed by Olympus Corporation filed Critical Olympus Corporation
Priority to JP2009542570A priority Critical patent/JP5566111B2/ja
Priority to EP08851485A priority patent/EP2218792A4/en
Priority to CN2008801248666A priority patent/CN101952460A/zh
Publication of WO2009066695A1 publication Critical patent/WO2009066695A1/ja
Priority to US12/782,537 priority patent/US8754204B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples

Abstract

本発明は、糞便中の大腸剥離細胞等の哺乳細胞由来の核酸等を効率よく回収することが可能な糞便試料を、煩雑な操作を必要とせずに調製し得る方法、該方法に用いられる糞便試料調製用溶液及び採便用キット、並びに、該方法により調製された糞便試料を用いて糞便中の核酸を回収し解析する方法を提供する。より具体的には糞便試料から核酸を効率よく回収するための糞便試料の調製方法であって、採取された糞便を、水溶性有機溶媒を有効成分とする糞便試料調製用溶液に混合させることを特徴とする、糞便試料の調製方法、該方法に用いられる糞便試料調製用溶液、採便用キット、該調製方法により調製された糞便試料中から、腸内常在菌由来の核酸と腸内常在菌以外の生物由来の核酸とを同時に回収することを特徴とする核酸回収方法、並びに、該核酸回収方法により回収された核酸を解析することを特徴とする核酸解析方法である。
PCT/JP2008/071023 2007-11-20 2008-11-19 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット WO2009066695A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2009542570A JP5566111B2 (ja) 2007-11-20 2008-11-19 Ｒｎａ回収方法
EP08851485A EP2218792A4 (en) 2007-11-20 2008-11-19 PROCESS FOR PREPARATION OF A KOTPROBE, SOLUTION FOR PREPARATION OF A KOTPROBE AND KOTSAMMELKIT
CN2008801248666A CN101952460A (zh) 2007-11-20 2008-11-19 用于制备粪便样品的方法、制备粪便样品用溶液和采集粪便用试剂盒
US12/782,537 US8754204B2 (en) 2007-11-20 2010-05-18 Method for preparing stool sample, solution for preparing stool sample, and kit for collecting stool

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007300298 2007-11-20
JP2007-300298 2007-11-20
JP2008080084 2008-03-26
JP2008-080084 2008-03-26

Related Child Applications (1)

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US12/782,537 Continuation US8754204B2 (en) 2007-11-20 2010-05-18 Method for preparing stool sample, solution for preparing stool sample, and kit for collecting stool

Publications (1)

Publication Number Publication Date
WO2009066695A1 true WO2009066695A1 (ja) 2009-05-28

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US (1) US8754204B2 (ja)
EP (1) EP2218792A4 (ja)
JP (1) JP5566111B2 (ja)
CN (1) CN101952460A (ja)
WO (1) WO2009066695A1 (ja)

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WO2010064628A1 (ja) * 2008-12-05 2010-06-10 オリンパス株式会社 核酸含有試料の調製方法、試料調製用溶液、及び核酸の解析方法
WO2010064634A1 (ja) * 2008-12-05 2010-06-10 オリンパス株式会社 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット
EP2314677A1 (en) * 2008-07-23 2011-04-27 Olympus Corporation Method for collection of nucleic acid from fecal sample, method for analysis of nucleic acid, and apparatus for treatment of fecal sample
CN112033743A (zh) * 2020-07-16 2020-12-04 上海市东方医院（同济大学附属东方医院） 一种帕金森粪便液提取装置
CN112033743B (zh) * 2020-07-16 2024-04-30 上海市东方医院（同济大学附属东方医院） 一种帕金森粪便液提取装置

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WO2013039477A1 (en) 2011-09-12 2013-03-21 University Of South Alabama Non-invasive methods of detecting target molecules
CA2848762C (en) 2011-09-14 2021-07-27 Queen's University At Kingston Method for treatment of disorders of the gastrointestinal system
US9744155B2 (en) 2012-03-28 2017-08-29 Ixcela, Inc. IPA as a therapeutic agent, as a protective agent, and as a biomarker of disease risk
JP6670765B2 (ja) 2014-03-07 2020-03-25 ディーエヌエー ジェノテック インク 生物試料中の核酸を安定化する組成物および方法
US9528105B2 (en) 2014-09-04 2016-12-27 Techlab, Inc. Nucleic acid extraction using organic solvents to remove inhibitors
US20210146352A1 (en) * 2017-07-24 2021-05-20 Epitope Diagnostics, Inc. Fecal sample collection and analyte extraction device and method
CN107312717A (zh) * 2017-08-08 2017-11-03 苏州阿迪康生物科技有限公司 一种粪便类样本的保存方法、保存用溶液、制备方法及应用
CN108893523A (zh) * 2018-05-31 2018-11-27 厦门安捷致善医学数据科技有限公司 一种粪便样品常温保存液
CN109517883A (zh) * 2018-12-05 2019-03-26 广州市达信智能科技有限公司 一种人粪便样本中脱落细胞的保存液
US11041847B1 (en) 2019-01-25 2021-06-22 Ixcela, Inc. Detection and modification of gut microbial population
CN111826371A (zh) * 2019-04-15 2020-10-27 上海锐翌生物科技有限公司 粪便核酸保存液及其制备方法和应用
JP2022535747A (ja) * 2019-05-28 2022-08-10 ケース ウエスタン リザーブ ユニバーシティ Ｄｎａのメチル化を保存するための組成物および方法
CN111044323A (zh) * 2020-03-03 2020-04-21 贵州里定医疗网络科技股份有限公司 人体大便常规检测液基预处理采样瓶

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Publication number Priority date Publication date Assignee Title
EP2314677A1 (en) * 2008-07-23 2011-04-27 Olympus Corporation Method for collection of nucleic acid from fecal sample, method for analysis of nucleic acid, and apparatus for treatment of fecal sample
EP2314677A4 (en) * 2008-07-23 2011-12-14 Olympus Corp METHOD OF TAKING NUCLEIC ACID FROM A CHAIR SAMPLE, NUCLEIC ACID ANALYSIS METHOD AND DEVICE FOR TREATING CHAIR SAMPLES
WO2010024251A1 (ja) * 2008-08-26 2010-03-04 オリンパス株式会社 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット
WO2010064628A1 (ja) * 2008-12-05 2010-06-10 オリンパス株式会社 核酸含有試料の調製方法、試料調製用溶液、及び核酸の解析方法
WO2010064634A1 (ja) * 2008-12-05 2010-06-10 オリンパス株式会社 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット
CN112033743A (zh) * 2020-07-16 2020-12-04 上海市东方医院（同济大学附属东方医院） 一种帕金森粪便液提取装置
CN112033743B (zh) * 2020-07-16 2024-04-30 上海市东方医院（同济大学附属东方医院） 一种帕金森粪便液提取装置

Also Published As

Publication number Publication date
EP2218792A4 (en) 2010-12-08
US8754204B2 (en) 2014-06-17
CN101952460A (zh) 2011-01-19
JPWO2009066695A1 (ja) 2011-04-07
US20100248250A1 (en) 2010-09-30
JP5566111B2 (ja) 2014-08-06
EP2218792A1 (en) 2010-08-18

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