WO2008106821A1 - Composition médicinale chinoise et extrait de larves associé et leur préparation - Google Patents

Composition médicinale chinoise et extrait de larves associé et leur préparation Download PDF

Info

Publication number
WO2008106821A1
WO2008106821A1 PCT/CN2007/000707 CN2007000707W WO2008106821A1 WO 2008106821 A1 WO2008106821 A1 WO 2008106821A1 CN 2007000707 W CN2007000707 W CN 2007000707W WO 2008106821 A1 WO2008106821 A1 WO 2008106821A1
Authority
WO
WIPO (PCT)
Prior art keywords
extract
water
hours
extraction
ethanol
Prior art date
Application number
PCT/CN2007/000707
Other languages
English (en)
Chinese (zh)
Inventor
Yiling Wu
Original Assignee
Hebei Yiling Medicine Research Institute Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hebei Yiling Medicine Research Institute Co., Ltd. filed Critical Hebei Yiling Medicine Research Institute Co., Ltd.
Priority to RU2009132927/15A priority Critical patent/RU2427381C2/ru
Priority to KR1020097020651A priority patent/KR101082007B1/ko
Priority to PCT/CN2007/000707 priority patent/WO2008106821A1/fr
Priority to CN2007800520061A priority patent/CN101657208B/zh
Publication of WO2008106821A1 publication Critical patent/WO2008106821A1/fr
Priority to HK10102924.5A priority patent/HK1137661A1/xx

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods
    • A61K35/646—Arachnids, e.g. spiders, scorpions, ticks or mites
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/62—Leeches; Worms, e.g. cestodes, tapeworms, nematodes, roundworms, earth worms, ascarids, filarias, hookworms, trichinella or taenia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods
    • A61K35/648—Myriapods, e.g. centipedes or millipedes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
    • A61K36/725—Ziziphus, e.g. jujube
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis

Definitions

  • the present invention relates to a traditional Chinese medicine composition for treating cardiovascular and cerebrovascular diseases, an extract thereof, and a preparation method thereof. Background technique
  • Coronary atherosclerotic heart disease refers to the formation of plaques in the coronary artery wall, resulting in stenosis or obstruction of the vascular lumen, causing myocardial ischemia and hypoxia. It is classified as ischemic heart disease with coronary artery spasm.
  • Tongxinluo capsule which has unique curative effect on diseases such as coronary heart disease, angina pectoris and cerebral thrombosis.
  • Tongxinluojiao has obtained the Chinese patent (ZL01131203. 3), which consists of scorpion, leeches, cockroaches, earthworms, cockroaches, ginseng, frankincense, red peony, scent, sandalwood, borneol and jujube kernel.
  • Tongxinluo capsule has a good effect in the treatment of coronary heart disease, angina pectoris, especially variant angina pectoris.
  • the traditional direct pulverization method is generally used in the existing production processes.
  • the insect medicine is made into a medicine powder.
  • the conventional pulverization process has the following problems: 1) The cell structure of the worm drug cannot be destroyed.
  • the active ingredient in the medicinal material is difficult to fully release and dissolve through the cell membrane; 2) the temperature of the material during the pulverization process due to the influence of mechanical heat energy Up to 80 °C, which has a negative impact on the stability of the active ingredients of the protein of the insecticide; 3) The degree of difficulty in crushing different parts of the insect medicine is different, the mixing is uneven due to the difference of the particles, and the quality of the product is affected. Uniformity.
  • CN1593623A (Application No.: 200410049615.
  • X) discloses a Tongxinluo Orally Disintegrating Tablet and a preparation method thereof, wherein the orally disintegrating tablet is composed of water extract of scorpion, leeches, mites, earthworms, cockroaches 15 ⁇ 70 parts by weight, ginseng, erythraea ethanol extract 1-12 parts by weight, borneol inclusion complex 10 - 100 parts by weight and medicinal excipients.
  • the preparation method is characterized in that: the macroporous resin adsorption technology is adopted for the removal of impurities of the botanical material, and the five kinds of insect medicines are extracted by ultrasonic vibration and concentrated by a rotary doctor blade method.
  • Ultrasonic extraction is applied to five kinds of insect medicines. Since the extraction temperature is room temperature, some active ingredients that require high temperature extraction are impossible to obtain by ultrasonic extraction alone. Therefore, an unsuitable preparation method not only affects the exertion of the drug effect, but also causes waste of precious insect medicines.
  • the preparation method of the traditional Chinese medicine composition for treating cardiovascular and cerebrovascular diseases comprises: weighing the whole cockroach, leeches, cockroaches, earthworms, cockroaches, ginseng, frankincense, red peony, fragrant, sandalwood according to the prescription amount Extracting the above-mentioned bulk drug, extracting the extract, and preparing the extract; wherein the extraction of the whole cockroach, leeches, cockroaches, earthworms, cockroaches includes the following Steps: (1) pulverize the insect drug into a coarse powder; (2) add 8-12 times the amount of water to the coarse powder, raise the temperature to 25 - 65 ° C, extract 1 - 3 times, each time 1-5 hours , separating the extract and the dregs; (3) adding 8-12 times of water to the dregs, heating to 70-100 ° C, extracting 1 - 3 times, each time 1-3 hours, separating the extract and the medicine (4) Combine the extract obtained in the above step
  • the invention also provides a traditional Chinese medicine composition product prepared according to the above preparation method, wherein the dosage form used in the product is selected from the group consisting of: a tablet, an orally disintegrating tablet, a dispersible tablet, an effervescent tablet, a capsule, a pill, a granule Any one of a dose, an oral solution, a pellet, a microcyanide, and a spray.
  • the dosage form used in the product is selected from the group consisting of: a tablet, an orally disintegrating tablet, a dispersible tablet, an effervescent tablet, a capsule, a pill, a granule Any one of a dose, an oral solution, a pellet, a microcyanide, and a spray.
  • the present invention also provides an insect medicine extraction method, wherein the insect medicine is composed of whole cockroaches, leeches, cockroaches, earthworms, cockroaches, and includes the following extraction steps: (1) worm medicine Crushed into coarse powder; (2) Add 8 - 12 times of water to the coarse powder, raise the temperature to 25 - 65 °C, extract 1 - 3 times, each time for 1 - 5 hours, separate the extract and dregs; 3) Add 8-12 times of water to the dregs, raise the temperature to 70-100 ° C, extract 1 - 3 times, each time 1-3 hours, separate the extract and dregs; (4) Combine the above steps The obtained extract was concentrated and dried to obtain an insect extract.
  • the insect medicine is composed of whole cockroaches, leeches, cockroaches, earthworms, cockroaches, and includes the following extraction steps: (1) worm medicine Crushed into coarse powder; (2) Add 8 - 12 times of water to the coarse powder, raise the temperature to
  • the present invention also provides an insect drug extract prepared according to the above extraction method, wherein the color of the insect drug extract is brownish yellow to blackish yellow, and the gas is salty and salty.
  • the color of the insect drug extract is brownish yellow to blackish yellow
  • the gas is salty and salty.
  • the traditional Chinese medicine composition of the present invention comprises: ginseng 3- 10 g, leech 3- 11 g, soil aphid 5-10 g, frankincense 1 to 5 g, red oak 3-9 g, 'decocting 1 to 5 g, 1 to 5 grams of sandalwood, 3 - 9 grams of whole glutinous rice, 3 - 12 grams of medlar, 1 - 3 grams of medlar, 1 - 7 grams of water, and 3 - 10 grams of jujube.
  • ZL01131203.3
  • the five herbs of the leeches, the cockroaches, the cockroaches, the earthworms, and the cockroaches may be extracted separately or together.
  • the extraction steps of the insect medicine are described in detail as follows:
  • Step (1) The above-mentioned insect medicine is pulverized into a coarse powder of 18-40 mesh, preferably a coarse powder of 24 mesh.
  • the step may be carried out by low temperature water extraction, low temperature dynamic extraction or ultrasonic extraction.
  • the extraction temperature is preferably about 50 °C.
  • the low temperature dynamic extraction is used, the extraction process is performed under reduced pressure.
  • the degree of vacuum may be 0.05 to 0.1 MPa, and the mixture is simultaneously stirred to boil the water under low temperature conditions, thereby allowing extraction. The process goes faster and more completely.
  • the output power and oscillation frequency of the ultrasonic extraction device should be appropriately controlled.
  • the output power of the ultrasonic extraction device is feasible in the range of 200-10000 W, preferably 250-8000 W, more preferably 1000-6000 W or 2000- 6000W
  • the oscillation frequency of the ultrasonic extraction equipment is feasible range of 20-80KHz, preferably 20-60KHz, more preferably 30-50KHZ
  • 200-2000 20-80KHz can be used under laboratory conditions
  • 2000 can be used in industrial production process - 10000 W, 20 - 80 KHz, wherein, preferably, 3000-8000 W, 30-60 KHz, and the temperature of the extract is controlled within a desired range.
  • the extraction temperature of the ultrasonic extraction is preferably 30 - 5 (TC, more preferably about 40 °C.
  • Step (3) adding 8 - 12 times the amount of water to the dregs obtained in the step (2), preferably 10 times the amount of water; heating to 70 - 100 ° C, preferably 95 ° C; extracting 1 - 3 times, preferably 2 times; each time 1
  • Step (4) Combine the extract obtained in the above step, and obtain the insect extract by concentration and drying.
  • the concentration process described in this step is preferably carried out at 50 to 60 ° C to concentrate the extract into a density of 1. 05 - 1. 10g / cffi 3 ; the drying process is preferably 50 - 60
  • the resulting clear paste was dried under reduced pressure at °C.
  • the extraction of the ginseng may be, but is not limited to, any one of the following methods:
  • the extracts are combined, filtered, and ethanol is recovered from the extract, and the residue obtained by recovering the ethanol is concentrated or dried or spray-dried to obtain a ginseng extract.
  • red peony take medicine with 40-90% ethanol under reflux for 4-12 times the amount of extracted I- 3 times, 1-2 hours each time, the combined extracts were filtered, recovering ethanol from the extract to no alcohol odor
  • Add 6-8 times the amount of medicinal material to dissolve it Pass the solution through the ruthenium-treated macroporous adsorption resin column, first wash it with 3-8 column volumes of water until colorless, discard the water wash solution, and then use 3- 8
  • the column volume is eluted with 30-90% ethanol, and the obtained eluate is recovered from ethanol, concentrated and dried or spray-dried to obtain an extract of red peony.
  • the extraction of the jujube kernel may be, but is not limited to, any one of the following methods:
  • the obtained eluate is recovered from ethanol, concentrated under reduced pressure or spray-dried to obtain an extract of jujube kernel.
  • the extraction of the reduced fragrance may be, but is not limited to, any one of the following methods:
  • the extraction of the sandalwood may be, but is not limited to, any one of the following methods: 1. Take the sandalwood medicinal herbs, distill with 4 - 15 times the amount of water, extract the volatile oil for more than 4 hours, collect the volatile oil, and obtain the sandalwood extract.
  • sandalwood herbs take sandalwood herbs, using supercritical C0 2 extraction, add 10 - 50% of the amount of 70 - 100% ethanol as an entrainer, collect the extract, dry, to make sandalwood extract.
  • the extraction of the malt-like scent may be, but is not limited to, any one of the following methods:
  • the inclusion compound of the borneol can be prepared by the following method: taking the borneol medicine according to the prescription amount, pulverizing to 60 mesh for use; taking another 1-3 times the amount of borneol Polyethylene glycol-6000, heated and melted at 60-80 ° C, and then added borneol fine powder, stirred rapidly, chilled after low temperature cooling and pulverized into a fine powder, for example, 60 mesh, to prepare a borneol clathrate.
  • the insecticide-like medicine, the rest are preferably extracted or prepared according to the following steps:
  • the macroporous adsorption resin column is selected from the group consisting of: D101, NKA-9, DA201, DM130, WLD-3, HPD-400, HPD-450, HPD- Any one of 500, AB-8; the alumina column may be an acid alumina column, Neutral alumina column or basic alumina column.
  • the ginseng extract contains not less than ginseng total saponin
  • the total extract of red peony root contains not less than 15 - 70%; and the extract of jujube kernel contains not less than 10 - 60% of total saponin.
  • the scent, sandalwood and savory may be separately extracted, or two or three of them may be extracted together.
  • the traditional Chinese medicine composition product provided by the invention is prepared according to the above method, and the dosage form used in the product may be a tablet, an orally disintegrating tablet, a dispersible tablet, an effervescent tablet, a capsule, a pill, a granule, Any of a dosage form such as an oral solution, a pellet, an elixir, or a spray.
  • Excipients used in this product are selected from the group consisting of: disintegrants, fillers, lubricants, binders, adsorbents, dispersants, surfactants, suspensions, preservatives, colorants, antioxidants, suspending agents, One or a mixture of one or more of a wetting agent, a solubilizer, a propellant, and a coating agent.
  • the insect medicine is composed of whole mites, leeches, mites, earthworms, and earthworms.
  • the five-flavored drugs can be extracted separately or together. The extraction step of the insect drug has been described in detail in the foregoing, and therefore will not be repeated here.
  • the invention adopts a combination of low-temperature extraction and high-temperature extraction to fully extract the insect medicine, the obtained extract has a greatly improved pharmacological effect than the simple low-temperature extraction, and thus can be greatly improved. Significantly reduce the amount of insecticides.
  • the traditional Chinese medicine composition product provided by the invention has the characteristics of advanced technology, more effective curative effect and small dosage. It can be seen from the pharmacodynamic comparison experiment that the traditional Chinese medicine composition product provided by the invention has more ideal effects against myocardial ischemia and anti-platelet aggregation, treatment of coronary heart disease, angina pectoris, and heart and blood stasis. detailed description
  • Example 1 The method and product thereof provided by the present invention are further illustrated by the following examples, but the invention is not limited thereby.
  • Example 1
  • This example illustrates: an insect drug extract prepared according to the method provided by the present invention and A capsule of a pharmaceutical composition.
  • Leeches were taken by prescription medicine 2075g, Eupolyphaga herbs 1375 g, 275 g of herbs centipede, scorpion 1375 g herbs, herbs cicada 1375 g, pulverized to 24 mesh coarse powder, to 10 times the amount of injection water, warmed to 60 ° C, the dynamic The extract and the dregs were separated by extracting for 3 hours. Eight times the amount of water was again injected into the obtained dregs, and the temperature was raised to 60 ° C, and the mixture was dynamically extracted for 2 hours to separate the extract and the dregs.
  • the ginseng medicinal material was taken for 1,100, and the ginseng medicinal materials were extracted three times with 10 times, 8 times and 6 times of 70% ethanol.
  • the extraction time was 1 hour, 2 hours and 1 hour respectively. 95 ° C;
  • 950g of Radix Paeoniae Alba was taken and pulverized into coarse powder. It was extracted three times with 10 times, 8 times and 6 times of 50% ethanol. The extraction time was 2 hours, 1 hour and 1 hour respectively. The temperature was 95 ° C, the extracts were combined, and ethanol was recovered. The obtained liquid was concentrated to a density of 1.18 g/cra 3 (55 ° C), and spray-dried to obtain 250 g of erythritol extract.
  • 925g of jujube seed medicine was pulverized, and it was smashed by 8 times, 8 times and 6 times of 80°/.
  • the ethanol was refluxed for 3 times, the extraction time was 2 hours, 1 hour and 1 hour, respectively, the extraction temperature was 95 ° C, the extracts were combined, the ethanol was recovered to an alcohol-free taste, and the obtained liquid was concentrated to a density of 1.18 g/cm 3 (50
  • the clear paste of °C was spray-dried to obtain 130g of the alcohol extract of jujube.
  • Example 2 Combine the above-mentioned insects, ginseng, red peony, jujube kernel, scent, sandalwood extract, granulate with 70% ethanol, then add the inclusion compound of emulsified milk powder, borneol and volatile oil, mix, fill the rubber ⁇ 20,000 granules, that is, the traditional Chinese medicine composition product S 1 prepared by the method of the present invention is obtained.
  • Example 2
  • This example illustrates: an insecticide extract prepared according to the method of the present invention and a tablet of the same.
  • the aqueous extract was first concentrated to a density of 1.18 g/cm 3 (4 (TC)), and then dried under reduced pressure at a low temperature to obtain leeches,
  • the total water extract of the soil mites, cockroaches, cockroaches and cockroaches was 1175 g.
  • the ginseng medicinal material was ll OOg, and the ginseng medicinal materials were extracted three times with 10 times, 8 times and 6 times of 70% ethanol.
  • the extraction time was 2 hours, 2 hours and 1 hour respectively.
  • the extract was combined at 95 ° C, and the ethanol was recovered to an alcohol-free taste.
  • the resulting liquid was concentrated to a clear density of 1.0 g/cm 3 (50 ⁇ ), and spray-dried to obtain 220 g of the ethanol extract of ginseng.
  • 925g of jujube seed was taken and pulverized. It was extracted three times with 10 times, 8 times and 6 times of water. The extraction time was 2 hours, 1 hour and 1 hour respectively, and the extraction temperature was 99 °C. , combined extracts. Concentrate it to 2 times the amount of medicinal material (ml/g), add ethanol to the obtained concentrated solution at a temperature of 60 ° C to make the ethanol concentration reach 80%, let stand for 12 hours, and take the supernatant to recover ethanol to no The mellow taste was further concentrated to a clearing paste having a density of 1.10 g/cm 3 (measured at 50 ° C), and spray-dried to obtain 105 g of the ethanol extract of Suanzaoren.
  • the above-mentioned insect medicine, ginseng, red peony, jujube kernel, scented, sandalwood extract is pulverized into a fine powder of 80 mesh, uniformly mixed with the fine powder of the emulsified milk, granulated with 70% ethanol, dried, sprayed
  • the borneol and volatile oil dissolved in an appropriate amount of ethanol were sealed for 20,000 sheets by sealing for half an hour to obtain a traditional Chinese medicine composition product prepared by the method of the present invention.
  • This example illustrates: an insecticide extract prepared according to the method of the present invention and a soft gelatin agent of a traditional Chinese medicine composition.
  • Drying equipment and conditions first concentrate the aqueous extract into a clear paste (40 ° C) with a density of 1.10 g / cm 3 , and then dry at a low temperature (55 ° C) under reduced pressure to obtain leeches, soil mites, mites, and cockroaches. , the total water extract of sputum is 1170g.
  • ginseng medicinal materials were taken.
  • the ginseng medicinal materials were extracted three times with 10 times, 8 times and 6 times of 70% ethanol.
  • the extraction time was 2 hours, 2 hours and 1 hour respectively.
  • the extraction temperature was 95 ° C, combined extract, ethanol recovery to alcohol-free, add water to 10 times the amount of herbs, allowed to stand for 12 hours, filtered, the filtrate passed through the processed AB-8 macroporous adsorption resin column (by Wuxi Xuelang Pharmaceutical)
  • the chemical equipment factory produces), the ratio of ginseng medicinal materials to resin is 1: 2 (g/ral), and the diameter ratio of the resin column is 1:9.
  • the eluate is concentrated and spray dried to obtain a ginseng column. 40.5 g.
  • 925g of jujube seed was taken and pulverized into coarse powder. It was extracted three times with 8 times, 8 times and 6 times of 80% ethanol. The extraction time was 2 hours, 2 hours and 1 hour respectively. The temperature was 95 ° C, the extracts were combined, the ethanol was recovered to an alcohol-free taste, water was added to 10 times the amount of the drug (ml/g), allowed to stand for 12 hours, filtered, and the filtrate was passed through a treated D-101 macroporous adsorption resin column ( Produced by Wuxi Xuelang Pharmaceutical Chemical Equipment Factory), the ratio of the amount of jujube seed and resin is 1: 2 (g/ml), and the diameter ratio of the resin column is 1:8. Rinse the resin column with 4 column volumes of water until it is colorless, discard the water wash solution, and then rinse the resin column with 6 column volumes of 80% ethanol. The eluate is concentrated and spray dried to obtain the refined jujube kernel. 32g.
  • This example illustrates: an insecticide extract prepared according to the method of the present invention and a pill of a traditional Chinese medicine composition.
  • each herbicide was firstly subjected to two low-temperature dynamic extractions; 10 times and 8 times of water were added respectively, and the extraction temperature was 6 (TC, and the extraction time was 3 hours and 2 hours, respectively, and the extract and the dregs were separated; The dregs were subjected to two heat extractions; 10 times and 8 times of water were added to the dregs respectively, and the extraction temperature was 98 ° C, and the extraction time was 2 hours and 1 hour respectively; the four aqueous extracts obtained were combined.
  • the aqueous extract was first concentrated to a clearing paste (40 ° C) having a density of 1.08 g/cm 3 , and then dried under reduced pressure at a low temperature (50 ° C).
  • the worm extract was prepared by the above method. 481 g of aqueous extract of leeches, 262 g of aqueous extract of locusts, 50 g of water extract of hydrazine, 255 g of water extract of cockroach, and 152 g of water extract of cockroach.
  • the extract was a total of 1200 g.
  • ginseng medicinal materials were taken.
  • the ginseng medicinal materials were extracted three times with 10 times, 8 times and 6 times of 70% ethanol.
  • the extraction time was 2 hours, 2 hours and 1 hour respectively.
  • the extraction temperature was At 95 °C, combine the extracts, recover the ethanol to an alcohol-free taste, add water to 10 times the amount of the drug, let stand for 12 hours, filter, and pass the filtrate through the processed AB-8 macroporous adsorption resin column (by Wuxi Xuelang Pharmaceutical) Produced by Chemical Equipment Factory), the ratio of ginseng medicinal materials to resin is 1: 2 (g/ml), and the diameter ratio of the resin column is 1:9.
  • the resulting eluate is passed through an alumina column (ginseng medicinal material and oxidation). 5 ⁇ The ginseng column purified product 34. 5g. The ginseng column purified product was obtained. 950g of red peony root was taken according to the prescription, pulverized into coarse powder, and extracted three times with 10 times, 8 times and 6 times of 50% ethanol, and the extraction time was 2 hours, 1.5 hours and 1 hour respectively. The extraction temperature was 95 ° C, the combined extracts were recovered, and the obtained supernatant was passed through a treated AB-8 macroporous resin column.
  • the ratio of the amount of the red peony to the resin is 1: 2. 3 (g/ml), and the diameter ratio of the resin column is 1:10.
  • 925g of jujube seed was taken and pulverized. It was extracted three times with 8 times, 8 times and 6 times of 80% ethanol. The extraction time was 2 hours, 2 hours and 1 hour respectively. The extraction temperature was 95. °C, the extracts were combined, and the ethanol was recovered to an alcohol-free taste.
  • the obtained supernatant was passed through a treated HPD-400 macroporous resin column (manufactured by Zhangzhou Baoen Chemical Co., Ltd.).
  • the ratio of the medicinal material to the resin was 1: 2 (g/ml), and the diameter ratio of the resin column was 1:8. Rinse the resin column with 5 times column of water until colorless. Discard the water wash solution, then wash it with 7 times column volume 80% ethanol.
  • the eluate is concentrated and dried to obtain 34g of refined jujube kernel.
  • the comparative example illustrates: a capsule of a traditional Chinese medicine composition prepared by a method in which a medicinal powder is used as a medicine.
  • the target insect medicine powder is 318g.
  • the red peony medicinal material was taken in 47.5 g, pulverized, extracted with 10, 8 and 6 times of 50% ethanol for 3 times, and the extraction time was 2, 1, and 1 hour respectively, and the combined extracts were recovered, and the ethanol was recovered. 8 ⁇ The residue was concentrated and dried to obtain red peony extract 12. 8g.
  • the jujube seed medicine 46 25g, crushed, extracted with 10, 8 and 6 times 80% ethanol for 3 times, the extraction time is 2, 2, 1 hour respectively, the combined extracts recover ethanol, and the residue obtained is concentrated and dried. 5 ⁇ The sour jujube kernel extract 6. 5g.
  • the comparative example illustrates: low temperature ultrasonic extraction of the insect medicine, and the obtained extract is used to prepare a traditional Chinese medicine composition.
  • This comparative example illustrates a capsule of a traditional Chinese medicine composition prepared by a method in which a medicinal powder is used as a medicine.
  • the drug is administered in an amount of 1. 2 g of crude drug / Kg, 0.6 g of crude drug / Kg and 0.3 g of crude drug / Kg; 1 - 3 prepared capsules Dl, D2 and D3, the amount of which was 0. 6 g of crude drug / Kg; another sham operation group and model control group.
  • Experimental animals SD rats, clean grade, male and female, 112, weighing 220-240 g, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., license number: SCXK (Beijing) 2002-0003.
  • Modeling and index detection methods After 30 minutes of the last administration, urethane was anesthetized, and the pre-operative II lead electrocardiogram was measured. The skin was cut along the midline of the left clavicle for about 2 cm, and the blunt dissection muscles were interposed between the 3rd and 4th intercostals.
  • the excess dye was rinsed with distilled water, the infarcted area and the non-infarcted area were separated and weighed, and the myocardial infarct range was calculated.
  • the stained myocardial sheet was photographed using an image analyzer. The total area of the myocardium and the area of the infarct area, leaving a typical ischemic myocardium picture, calculated the percentage of area of myocardial infarction.
  • t is expressed as the mean soil standard deviation ( ⁇ SD), and the t test is performed using SPSS 11. 5 software.
  • t is the approximate form of the standard normal distribution and is the basic concept of statistics.
  • Example 1 Use the capsule S1 prepared in Example 1, refer to the human dose of 4.557g of crude drug/day, that is, 0.076 g of crude drug/kg body weight (standard weight 60kg), set three The doses were 2.4g crude drug/kgjday, 1.2g crude drug/kgjday and 0.6g crude drug/kgj day c.
  • the capsules Dl, D2 and D3 prepared in the proportions of 1-3 were used, and the dosage was 1.2g of crude drug. /kgjday.
  • Another model group and sham operation group are set up.
  • Modeling and index detection After anesthesia with chloral hydrate, fix it on the side. Except for the sham operation group, the other groups underwent the following operations: Cut a small opening at the midline of the eyelid and the external auditory canal, expose the diaphragm, and use the hemostatic forceps to separate the diaphragm and separate it to the sides, taking care not to damage the facial nerve. Exposed to the majority of the squamous bone, and then drilled about 2 in front of the anterior and posterior zygomatic arches. Under the surgical microscope, open a small cranial window with a small straight-legged forceps to reveal the brain. artery.
  • the coronal cut 4 knives were divided into 5 pieces.
  • the first knife is at the midpoint of the front pole of the brain and the line of intersection
  • the second knife is at the optic intersection
  • the third knife is at the funnel handle
  • the fourth knife is between the funnel handle and the leaf tail.
  • the brain slices were stained with red tetrazolium (TTC), and the normal tissues were stained red and the infarcted tissues were white. Each brain plane was projected onto a uniform density aluminum foil, and the infarct size was carefully excavated, and the infarct size (%) was taken as the percentage of the weight of the infarct tissue and the weight of the whole brain.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Epidemiology (AREA)
  • Engineering & Computer Science (AREA)
  • Insects & Arthropods (AREA)
  • Medical Informatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Cardiology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Vascular Medicine (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

L'invention concerne une méthode de préparation d'une composition médicinale chinoise, la composition étant préparée à partir de Semen Ziziphi Spinosae. L'invention concerne une méthode de préparation d'un extrait de larves, l'extrait étant préparé à partir de scorpion, de scolopandre, de Eupolyphaga Seu Steleophaga, de Periostracum Cicadae et de sangsue. L'invention concerne la composition médicinale chinoise et l'extrait obtenus selon lesdits procédés.
PCT/CN2007/000707 2007-03-05 2007-03-05 Composition médicinale chinoise et extrait de larves associé et leur préparation WO2008106821A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
RU2009132927/15A RU2427381C2 (ru) 2007-03-05 2007-03-05 Китайская лекарственная композиция, содержащиеся в композиции лекарственные экстракты насекомых и способы приготовления композиции
KR1020097020651A KR101082007B1 (ko) 2007-03-05 2007-03-05 한약 조성물, 이에 함유된 곤충 추출물 및 그의 제법
PCT/CN2007/000707 WO2008106821A1 (fr) 2007-03-05 2007-03-05 Composition médicinale chinoise et extrait de larves associé et leur préparation
CN2007800520061A CN101657208B (zh) 2007-03-05 2007-03-05 一种中药组合物、其所含虫类药提取物及其制备方法
HK10102924.5A HK1137661A1 (en) 2007-03-05 2010-03-19 A chinese medicine composition and the extract of worms thereof and their preparation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2007/000707 WO2008106821A1 (fr) 2007-03-05 2007-03-05 Composition médicinale chinoise et extrait de larves associé et leur préparation

Publications (1)

Publication Number Publication Date
WO2008106821A1 true WO2008106821A1 (fr) 2008-09-12

Family

ID=39737761

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2007/000707 WO2008106821A1 (fr) 2007-03-05 2007-03-05 Composition médicinale chinoise et extrait de larves associé et leur préparation

Country Status (5)

Country Link
KR (1) KR101082007B1 (fr)
CN (1) CN101657208B (fr)
HK (1) HK1137661A1 (fr)
RU (1) RU2427381C2 (fr)
WO (1) WO2008106821A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102210761A (zh) * 2010-04-08 2011-10-12 河北以岭医药研究院有限公司 一种中药组合物在制备治疗慢性肠系膜缺血药物中的应用
CN102210760A (zh) * 2010-04-08 2011-10-12 河北以岭医药研究院有限公司 一种中药组合物在制备治疗慢性附睾炎药物中的应用
CN102210758A (zh) * 2010-04-08 2011-10-12 河北以岭医药研究院有限公司 一种中药组合物在制备治疗强直性脊柱炎药物中的应用
CN112335811A (zh) * 2020-12-04 2021-02-09 宿州滋原科技咨询有限公司 一种食品杀菌保鲜专用鲜冻液及其制备方法
CN115413684A (zh) * 2022-09-22 2022-12-02 烟台凯多海洋生物研究院有限公司 一种香蒲提取物制备的同翅目昆虫驱除剂

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2496509C2 (ru) * 2012-01-24 2013-10-27 Государственное бюджетное образовательное учреждение высшего профессионального образования "Самарский государственный медицинский университет" Министерства здравоохранения Российской Федерации Способ получения средства, обладающего тонизирующей активностью
CN104337841B (zh) * 2013-08-08 2020-10-27 河北以岭医药研究院有限公司 一种蝉蜕中抗凝纤溶组分
KR101655263B1 (ko) * 2014-07-04 2016-09-07 원광대학교산학협력단 오공교의 제조방법, 상기 방법으로 제조된 오공교, 상기 오공교를 이용하여 오공교 약침을 제조하는 방법 및 상기 방법으로 제조된 오공교 약침
KR101661966B1 (ko) * 2014-10-10 2016-10-04 원광대학교산학협력단 퇴행성 신경계질환에 유효한 오공을 포함하는 복합약침의 제조방법 및 상기 방법으로 제조된 복합약침
KR101720325B1 (ko) * 2014-11-27 2017-03-28 이명지 신명주사제의 제조방법 및 상기 방법으로 제조된 신명주사제
RU2598748C1 (ru) * 2015-05-15 2016-09-27 Федеральное государственное автономное образовательное учреждение высшего образования "Белгородский государственный национальный исследовательский университет" (НИУ "БелГУ") Способ получения нанокапсул адаптогенов в альгинате натрия
CN107308372A (zh) * 2017-08-01 2017-11-03 李勇 一种治疗面部神经麻痹的药物及其制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1198332A (zh) * 1997-05-04 1998-11-11 吴以岭 治疗冠心病心绞痛的通心络胶囊及其制备方法
CN1709291A (zh) * 2004-06-18 2005-12-21 河北以岭医药研究院有限公司 超微通心络中药组合物及其制备方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1124153C (zh) * 2001-08-31 2003-10-15 石家庄以岭药业股份有限公司 一种治疗冠心病心绞痛的药物组合物
CN1278705C (zh) * 2004-06-22 2006-10-11 张晴龙 一种通心络口腔崩解片及其制备方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1198332A (zh) * 1997-05-04 1998-11-11 吴以岭 治疗冠心病心绞痛的通心络胶囊及其制备方法
CN1709291A (zh) * 2004-06-18 2005-12-21 河北以岭医药研究院有限公司 超微通心络中药组合物及其制备方法

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102210761A (zh) * 2010-04-08 2011-10-12 河北以岭医药研究院有限公司 一种中药组合物在制备治疗慢性肠系膜缺血药物中的应用
CN102210760A (zh) * 2010-04-08 2011-10-12 河北以岭医药研究院有限公司 一种中药组合物在制备治疗慢性附睾炎药物中的应用
CN102210758A (zh) * 2010-04-08 2011-10-12 河北以岭医药研究院有限公司 一种中药组合物在制备治疗强直性脊柱炎药物中的应用
CN112335811A (zh) * 2020-12-04 2021-02-09 宿州滋原科技咨询有限公司 一种食品杀菌保鲜专用鲜冻液及其制备方法
CN115413684A (zh) * 2022-09-22 2022-12-02 烟台凯多海洋生物研究院有限公司 一种香蒲提取物制备的同翅目昆虫驱除剂
CN115413684B (zh) * 2022-09-22 2023-10-13 烟台固特丽生物科技股份有限公司 一种香蒲提取物制备的同翅目昆虫驱除剂

Also Published As

Publication number Publication date
KR20100005064A (ko) 2010-01-13
CN101657208A (zh) 2010-02-24
HK1137661A1 (en) 2010-08-06
KR101082007B1 (ko) 2011-11-09
RU2009132927A (ru) 2011-04-10
RU2427381C2 (ru) 2011-08-27
CN101657208B (zh) 2011-10-19

Similar Documents

Publication Publication Date Title
WO2008106821A1 (fr) Composition médicinale chinoise et extrait de larves associé et leur préparation
CN101618161B (zh) 一种治疗血栓性静脉炎的中药组合物及其制备方法
JP2007505936A (ja) 循環器疾患および脳血管疾患の処置のための薬学的組成物
EP2345416B1 (fr) Composition pharmaceutique de purification des veines et sa méthode d'élaboration
CN1939438B (zh) 一种中药组合物、其所含虫类药提取物及其制备方法
CN1814170A (zh) 一种治疗心血管疾病的药物滴丸及其制备方法
CN102038940A (zh) 治疗心脑血管疾病的水蛭素组合药物
CN101584743A (zh) 一种治疗心脑血管疾病的中药复方丹参制剂的制备方法
CN101766788B (zh) 一种治疗脑血管病的药物组合物
CN1679697A (zh) 一种含有三七、瓜蒌皮、水蛭的治疗心血管疾病的中药制剂及其制备方法
CN1679698A (zh) 一种由三七、川芎制成的治疗心脑血管疾病的药物制剂及其制备方法
CN102961582B (zh) 一种复方中药超微粉分散体制剂及其制备方法
CN102895395A (zh) 一种大黄虫丸整合型新剂型制备技术及其生产方法
JP2017515902A (ja) 脂血及び体重を制御する薬物組成物とその応用法
KR20110064067A (ko) 키토산을 이용한 병풀의 면역 증진 성분의 식용 나노입자 및 그 제조방법, 그 나노입자를 함유한 식품, 음료 또는 의약품
CN105748553A (zh) 一种活血消肿解毒的中药组合物及其制备方法
CN103463204A (zh) 一种治疗便秘的药物及其制备方法
WO2021051261A1 (fr) Nouvelle utilisation de composition de médecine chinoise
CN114159489B (zh) 一种解毒散结中药组合物及其制备方法
CN103405724B (zh) 一种中药组合物及其制备方法
CN102940656B (zh) 治疗前列腺增生的药物组合物
CN101856403B (zh) 一种药物组合物及其制备方法和制药用途
CN114432393A (zh) 一种治疗冠心病稳定型心绞痛的中药组合物及其制备方法与应用
CN104069067B (zh) 一种防治缺血性心脑血管疾病的豆腐果苷干混悬剂及其制备方法与应用
CN1593574A (zh) 治疗心脑血管疾病的中药制剂及其制备方法

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 200780052006.1

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07720328

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: PI20093630

Country of ref document: MY

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 20097020651

Country of ref document: KR

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 2009132927

Country of ref document: RU

122 Ep: pct application non-entry in european phase

Ref document number: 07720328

Country of ref document: EP

Kind code of ref document: A1