WO2008023771A1 - RÉcipient DE CULTURE, ET PROCÉDÉ D'INTRODUCTION DE GÈNE ET PROCÉDÉ DE CULTURE CELLULairE UTILISANT LE RÉcipient - Google Patents

RÉcipient DE CULTURE, ET PROCÉDÉ D'INTRODUCTION DE GÈNE ET PROCÉDÉ DE CULTURE CELLULairE UTILISANT LE RÉcipient Download PDF

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Publication number
WO2008023771A1
WO2008023771A1 PCT/JP2007/066384 JP2007066384W WO2008023771A1 WO 2008023771 A1 WO2008023771 A1 WO 2008023771A1 JP 2007066384 W JP2007066384 W JP 2007066384W WO 2008023771 A1 WO2008023771 A1 WO 2008023771A1
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WO
WIPO (PCT)
Prior art keywords
container
culture
cells
cell
fragment
Prior art date
Application number
PCT/JP2007/066384
Other languages
English (en)
Japanese (ja)
Inventor
Hideto Chono
Junichi Mineno
Kazutoh Takesako
Takao Yoshida
Takashi Morimura
Kenji Sakai
Shin-Ichi Yamada
Noritsugu Yabe
Yuko Taguchi
Original Assignee
Takara Bio Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc. filed Critical Takara Bio Inc.
Publication of WO2008023771A1 publication Critical patent/WO2008023771A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture

Definitions

  • cycloolefin copolymer examples thereof include APPEL (registered trademark) (manufactured by Mitsui Chemicals, Inc.) and TOPAS (registered trademark) (manufactured by TICONA), which are resins obtained by copolymerizing ethylene and heterocyclic monoolefin.
  • APPEL registered trademark
  • TOPAS registered trademark
  • the component constituting the extracellular matrix is fibronectin, laminin, collagen, proteodalycan, or a fragment thereof. It is characterized by.
  • the first wall is formed as a film on the substrate and the inner surface of the container of the substrate in the form of a film. An effect is obtained.
  • the flexible resin low density polyethylene resin, ethylene vinyl acetate copolymer, polypropylene resin, ethylene propylene copolymer resin, polybutadiene resin, styrene butadiene copolymer resin, and hydrogenated resins thereof. , Polyurethane resins and the like, and mixtures of these resins.
  • the oxygen permeability required for the container wall of the container of the present invention varies depending on the amount of cells to be cultured and the area of the container wall through which oxygen permeates. That is, the oxygen permeability required for the container wall is proportional to the amount of cells to be cultured and inversely proportional to the area of the container wall through which oxygen permeates.
  • the component constituting the extracellular matrix is fibronectin, laminin, collagen, proteodalycan, or a fragment thereof.
  • Comparative Example 4 having the same form as Example 1 was obtained except that a commercially available polyester resin sheet having a thickness of 200 Hm was used as a petri dish-like sheet formed by vacuum forming.
  • a mononuclear cell fraction was separated from peripheral venous blood collected from a human and suspended in a medium (KBM540, manufactured by Kojin Bio Inc.).
  • the vector pQBI polII (neo—) in which is expressed was obtained.
  • pQBI polll (neo—) was deleted with the restriction enzyme Xhol, a DNA fragment containing a GFP expression unit was obtained under the control of the polll promoter, and the end was blunted using a DNAblunting kit (manufactured by Takara Bio Inc.).
  • Vector fragment obtained by digesting the retroviral vector plasmid pDON-AI (Takara Bio) with restriction enzymes Xhol and Sphl. The blunt end of the 58kbp is blunted using DNAblunting kit (Takara Bio).

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un récipient de culture présentant des propriétés de culture identiques ou supérieures à une boîte de pétri ou à un flacon en résine de styrène. Ledit récipient est hautement sécuritaire et approprié à la culture à des fins médicales. L'invention concerne également un procédé d'introduction de gène et un procédé de culture cellulaire utilisant tout deux le récipient. Sur la surface d'un corps de récipient recevant des cellules, au moins une partie entrant en contact avec les cellules est formée d'une résine de cyclooléfine.
PCT/JP2007/066384 2006-08-23 2007-08-23 RÉcipient DE CULTURE, ET PROCÉDÉ D'INTRODUCTION DE GÈNE ET PROCÉDÉ DE CULTURE CELLULairE UTILISANT LE RÉcipient WO2008023771A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2006227134A JP5659448B2 (ja) 2006-08-23 2006-08-23 培養容器及び同容器を用いた細胞培養方法
JP2006-227134 2006-08-23

Publications (1)

Publication Number Publication Date
WO2008023771A1 true WO2008023771A1 (fr) 2008-02-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/066384 WO2008023771A1 (fr) 2006-08-23 2007-08-23 RÉcipient DE CULTURE, ET PROCÉDÉ D'INTRODUCTION DE GÈNE ET PROCÉDÉ DE CULTURE CELLULairE UTILISANT LE RÉcipient

Country Status (2)

Country Link
JP (1) JP5659448B2 (fr)
WO (1) WO2008023771A1 (fr)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008048651A (ja) * 2006-08-23 2008-03-06 Takara Bio Inc 遠心用袋状容器及び同袋状容器を使用した遺伝子導入方法
JP2010167413A (ja) * 2009-01-23 2010-08-05 Millipore Corp 生物学的液体のための回路を提供するための方法および得られた回路
WO2011071074A1 (fr) * 2009-12-08 2011-06-16 株式会社ジェイ・エム・エス Élément poreux, procédé pour causer la porosité, et procédé pour fabriquer ledit élément poreux
WO2015170466A1 (fr) * 2014-05-09 2015-11-12 東洋製罐グループホールディングス株式会社 Récipient de culture multi-chambre et procédé de culture cellulaire
WO2015199118A1 (fr) * 2014-06-26 2015-12-30 日本ゼオン株式会社 Procédé pour favoriser la phosphorylation d'erk ou d'akt dans une cellule cultivée, procédé de culture cellulaire et promoteur de phosphorylation
WO2015199119A1 (fr) * 2014-06-26 2015-12-30 日本ゼオン株式会社 Procédé de culture de cellules adhérentes, récipient de culture et procédé de production de protéines
CN105658784A (zh) * 2013-12-18 2016-06-08 东洋制罐集团控股株式会社 培养容器、淋巴细胞的培养方法、培养容器的制造方法和固相化装置
WO2017010533A1 (fr) * 2015-07-16 2017-01-19 日本ゼオン株式会社 Procédé pour favoriser la production de matrice extracellulaire, procédé pour la culture de cellules et agent pour favoriser la production de matrice extracellulaire
JP2017079658A (ja) * 2015-10-29 2017-05-18 日本ゼオン株式会社 接着型細胞の培養方法、及びタンパク質の産生方法
EP3385368A4 (fr) * 2015-12-03 2019-05-08 Takara Bio Inc. Procédé de production de cellules souches mésenchymateuses
EP3690013A4 (fr) * 2017-09-29 2021-06-23 Zeon Corporation Instrument biochimique

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2542116C (fr) 2003-10-08 2015-01-27 Wilson Wolf Manufacturing Corporation Procedes et dispositifs pour la culture cellulaire utilisant des materiaux permeables au gaz
CN102015997B (zh) * 2008-05-05 2016-08-10 威尔森沃尔夫制造公司 细胞容器
EP2297295A4 (fr) 2008-07-08 2013-11-27 Wolf Wilson Mfg Corp Dispositif perfectionné de culture cellulaire perméable au gaz et procédé d'utilisation
JP5861419B2 (ja) * 2011-12-02 2016-02-16 大日本印刷株式会社 機能性フィルム付き細胞培養容器およびその製造方法
JP5994144B2 (ja) * 2013-10-06 2016-09-21 株式会社セレックス Ｎｋ細胞培養容器及びｎｋ細胞培養方法
EP3162891B1 (fr) * 2014-06-26 2019-12-11 Zeon Corporation Procédé pour promouvoir la différenciation de cellules en culture et promoteur de différenciation de cellules en culture
US20170137784A1 (en) * 2015-03-23 2017-05-18 Cellex Corporation NK Cell Culture Container And NK Cell Culture Method
JP2017081073A (ja) * 2015-10-30 2017-05-18 日本ゼオン株式会社 ガス透過性複合体及びその利用
JP6973082B2 (ja) * 2015-12-16 2021-11-24 日本ゼオン株式会社 接着型細胞の培養方法
JP6954121B2 (ja) * 2015-12-28 2021-10-27 日本ゼオン株式会社 培養細胞の分化促進方法及び培養細胞分化促進剤
WO2018207907A1 (fr) * 2017-05-12 2018-11-15 株式会社フコク Récipient de culture cellulaire
JP2018201403A (ja) * 2017-06-02 2018-12-27 日本ゼオン株式会社 幹細胞の培養方法

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WO1998055231A1 (fr) * 1997-06-02 1998-12-10 Aurora Biosciences Corporation Plaques a puits multiples a faible fond pour mesures de fluorescence sur echantillons biologiques et biochimiques
JP2005058103A (ja) * 2003-08-13 2005-03-10 Lymphotec:Kk 閉鎖系細胞培養用容器及び該容器を用いた細胞の増殖培養方法、ならびに該培養方法を用いた免疫治療剤、細胞増殖培養用キット
JP2005237274A (ja) * 2004-02-26 2005-09-08 Kenichiro Hatake 細胞培養方法、チャンバ及びそれを利用した細胞培養装置
JP2005271504A (ja) * 2004-03-26 2005-10-06 Japan Science & Technology Agency 樹脂発泡成形体の製造方法及び樹脂発泡成形体

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WO2000009168A1 (fr) * 1998-08-11 2000-02-24 The United States Of America, Represented By Department Of Health And Human Services Procede relatif a la transduction de cellules mammaliennes, et produits connexes

Patent Citations (4)

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WO1998055231A1 (fr) * 1997-06-02 1998-12-10 Aurora Biosciences Corporation Plaques a puits multiples a faible fond pour mesures de fluorescence sur echantillons biologiques et biochimiques
JP2005058103A (ja) * 2003-08-13 2005-03-10 Lymphotec:Kk 閉鎖系細胞培養用容器及び該容器を用いた細胞の増殖培養方法、ならびに該培養方法を用いた免疫治療剤、細胞増殖培養用キット
JP2005237274A (ja) * 2004-02-26 2005-09-08 Kenichiro Hatake 細胞培養方法、チャンバ及びそれを利用した細胞培養装置
JP2005271504A (ja) * 2004-03-26 2005-10-06 Japan Science & Technology Agency 樹脂発泡成形体の製造方法及び樹脂発泡成形体

Cited By (23)

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US8815597B2 (en) 2006-08-23 2014-08-26 Takara Bio Inc. Baglike container for centrifugation and method of gene transfer using the same
JP2008048651A (ja) * 2006-08-23 2008-03-06 Takara Bio Inc 遠心用袋状容器及び同袋状容器を使用した遺伝子導入方法
JP2010167413A (ja) * 2009-01-23 2010-08-05 Millipore Corp 生物学的液体のための回路を提供するための方法および得られた回路
WO2011071074A1 (fr) * 2009-12-08 2011-06-16 株式会社ジェイ・エム・エス Élément poreux, procédé pour causer la porosité, et procédé pour fabriquer ledit élément poreux
CN102753677A (zh) * 2009-12-08 2012-10-24 株式会社Jms 多孔性部件、多孔化方法和所述多孔性部件的制造方法
CN107267383A (zh) * 2013-12-18 2017-10-20 东洋制罐集团控股株式会社 培养容器的制造方法和固相化装置
US11884907B2 (en) 2013-12-18 2024-01-30 Toyo Seikan Group Holdings, Ltd. Culture container, method for culturing lymphocytes, culture-container production method, and solid-phasing apparatus
EP3421587A1 (fr) 2013-12-18 2019-01-02 Toyo Seikan Group Holdings, Ltd. Procédé de production de récipient de culture et appareil d'immobilisation
CN105658784B (zh) * 2013-12-18 2018-12-11 东洋制罐集团控股株式会社 培养容器、淋巴细胞的培养方法、培养容器的制造方法和固相化装置
EP3085767A4 (fr) * 2013-12-18 2017-11-01 Toyo Seikan Group Holdings, Ltd. Récipient de culture, procédé de culture de lymphocytes, procédé de production de récipient de culture, et appareil de mise en phase solide
CN105658784A (zh) * 2013-12-18 2016-06-08 东洋制罐集团控股株式会社 培养容器、淋巴细胞的培养方法、培养容器的制造方法和固相化装置
WO2015170466A1 (fr) * 2014-05-09 2015-11-12 東洋製罐グループホールディングス株式会社 Récipient de culture multi-chambre et procédé de culture cellulaire
JP2015213458A (ja) * 2014-05-09 2015-12-03 東洋製罐グループホールディングス株式会社 複室培養容器、及び細胞培養方法
JP2016146819A (ja) * 2014-06-26 2016-08-18 日本ゼオン株式会社 培養細胞内のｅｒｋまたはａｋｔのリン酸化亢進方法、および細胞の培養方法
JPWO2015199118A1 (ja) * 2014-06-26 2017-04-20 日本ゼオン株式会社 培養細胞内のｅｒｋまたはａｋｔのリン酸化亢進方法、細胞の培養方法、およびリン酸化亢進剤
JP5958658B2 (ja) * 2014-06-26 2016-08-02 日本ゼオン株式会社 培養細胞内のｅｒｋまたはａｋｔのリン酸化亢進方法、細胞の培養方法、およびリン酸化亢進剤
JP5867667B1 (ja) * 2014-06-26 2016-02-24 日本ゼオン株式会社 接着型細胞の培養方法、培養容器およびタンパク質の産生方法
WO2015199119A1 (fr) * 2014-06-26 2015-12-30 日本ゼオン株式会社 Procédé de culture de cellules adhérentes, récipient de culture et procédé de production de protéines
WO2015199118A1 (fr) * 2014-06-26 2015-12-30 日本ゼオン株式会社 Procédé pour favoriser la phosphorylation d'erk ou d'akt dans une cellule cultivée, procédé de culture cellulaire et promoteur de phosphorylation
WO2017010533A1 (fr) * 2015-07-16 2017-01-19 日本ゼオン株式会社 Procédé pour favoriser la production de matrice extracellulaire, procédé pour la culture de cellules et agent pour favoriser la production de matrice extracellulaire
JP2017079658A (ja) * 2015-10-29 2017-05-18 日本ゼオン株式会社 接着型細胞の培養方法、及びタンパク質の産生方法
EP3385368A4 (fr) * 2015-12-03 2019-05-08 Takara Bio Inc. Procédé de production de cellules souches mésenchymateuses
EP3690013A4 (fr) * 2017-09-29 2021-06-23 Zeon Corporation Instrument biochimique

Also Published As

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JP2008048653A (ja) 2008-03-06

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