WO1998030683A2 - Nuclear transfer with differentiated fetal and adult donor cells - Google Patents

Nuclear transfer with differentiated fetal and adult donor cells Download PDF

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Publication number
WO1998030683A2
WO1998030683A2 PCT/US1998/000002 US9800002W WO9830683A2 WO 1998030683 A2 WO1998030683 A2 WO 1998030683A2 US 9800002 W US9800002 W US 9800002W WO 9830683 A2 WO9830683 A2 WO 9830683A2
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Prior art keywords
cells
cell
disease
offspring
differentiated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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PCT/US1998/000002
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English (en)
French (fr)
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WO1998030683A3 (en
Inventor
Steven L. Stice
Jose Cibelli
James Robl
Paul Golueke
F. Abel Ponce De Leon
D. Joseph Jerry
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University of Massachusetts Boston
University of Massachusetts Amherst
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University of Massachusetts Boston
University of Massachusetts Amherst
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25123802&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1998030683(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to BR9806872-5A priority Critical patent/BR9806872A/pt
Application filed by University of Massachusetts Boston, University of Massachusetts Amherst filed Critical University of Massachusetts Boston
Priority to IL13082998A priority patent/IL130829A0/xx
Priority to JP53095898A priority patent/JP2001512964A/ja
Priority to EP98903349A priority patent/EP1015572B1/en
Priority to CN988027941A priority patent/CN1248288B/zh
Priority to DE69837270T priority patent/DE69837270T2/de
Priority to AU60145/98A priority patent/AU742363C/en
Priority to CA2277192A priority patent/CA2277192C/en
Priority to NZ336612A priority patent/NZ336612A/xx
Publication of WO1998030683A2 publication Critical patent/WO1998030683A2/en
Publication of WO1998030683A3 publication Critical patent/WO1998030683A3/en
Priority to IL130829A priority patent/IL130829A/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
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    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
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    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/04—Cells produced using nuclear transfer

Definitions

  • the present invention relates to cloning procedures
  • mammalian oocytes of the same species as the donor nuclei.
  • the nuclei are reprogrammed to direct the development of
  • CICM cultured inner cell mass cells
  • the cloned embryos can also be combined with fertilized
  • ES cells can be passaged in an undifferentiated
  • ES cells can be used as an in vi tro model for
  • Mouse ES cells can give rise to germline chimeras when
  • pluripotential embryonic cell lines from pig blastocysts pluripotential embryonic cell lines from pig blastocysts.
  • embryonic fibroblast feeder layers without the use of conditioned medium, and reportedly differentiate into
  • mouse ES cell lines derived from mouse ICMs isolation of mouse ES cell lines derived from mouse ICMs.
  • embryos are totipotent by nuclear transfer and that
  • ICMs isolated and cultured ICMs from 8 or 9 day bovine
  • fibroblast feeder cells instead of bovine uterus epithelial cells
  • animal stem cells which purportedly may be used to obtain
  • ICM inner cell mass
  • bovine ICM cells as donor nuclei
  • bovine pluripotent embryonic cells in nuclear transfer and the production of chimeric
  • granulosa cells adult cells could be used in a bovine
  • nuclei (less than 100), or with in vitro cell lines. It
  • transgenic mammals i.e., embryos, fetuses, offspring.
  • transgenic mammals by which a desired gene is inserted, removed or modified in a differentiated mammalian cell or
  • CICM cells for therapy or diagnosis.
  • CICM cells including human and ungulate CICM cells, for
  • organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation
  • the CICM cells may be used
  • NT embryos derived from NT embryos, fetuses or offspring, including
  • tissues may be used within the same species or across
  • vi tro e.g. for study of cell differentiation and for
  • Such therapies include by way of example
  • transgenic or genetically engineered CICM cells produced
  • the invention as nuclear donors for nuclear transplantation.
  • the present invention provides a
  • a mammal e.g., embryos, fetuses,
  • the method comprises:
  • NT unit develops into a fetus.
  • the cells, tissues and/or organs of the fetus are provided.
  • the present invention also includes a method of
  • mammals are Also provided by the present invention.
  • the present invention is preferably used for cloning
  • the present invention provides a
  • the method comprises:
  • the present invention provides improved procedures
  • differentiated fetal or adult, mammalian cells are differentiated fetal or adult, mammalian cells.
  • the nuclei are reprogrammed
  • fetuses and offspring or used to produce CICM cells.
  • cloned embryos can also be combined with fertilized
  • present invention uses differentiated cells.
  • transgenic embryonic cell lines could be readily derived
  • the present invention also allows simplification of
  • these cells can be clonally propagated without cytokines
  • transgenic embryos are obtained from the same according to the invention.
  • transgenic cloned embryos can be used to generate transgenic cloned embryos.
  • the present invention eliminates the need to
  • the present invention can also be used to produce
  • CICM cells fetuses or offspring which can be used, for
  • This process can provide a source of "materials" for many
  • hematopoietic chimerism can be used to avoid immunological
  • the present invention provides a
  • NT unit develops into a fetus.
  • the present invention also includes a method of
  • mammals are Also provided by the present invention.
  • the present invention is preferably used
  • the present invention further provides for the use of
  • the present invention provides a
  • the method comprises:
  • the NT units will be cultured to a size
  • Differentiated mammalian cells are those cells which
  • the differentiated cells are those from at least past the
  • the differentiated cells may be derived from ectoderm,
  • Mammalian cells including human cells, may be any suitable mammalian cells.
  • Mammalian cells including human cells, may be any suitable mammalian cells.
  • the present invention include, by way of example,
  • epithelial cells neural cells, epidermal cells,
  • keratinocytes hematopoietic cells, melanocytes
  • chondrocytes chondrocytes, lymphocytes (B and T lymphocytes) .
  • erythrocytes erythrocytes, macrophages, monocytes, mononuclear cells,
  • donor cells i.e., cells useful in
  • the subject invention may be obtained from any cell or
  • Fibroblast cells are an ideal cell type because they
  • Fibroblast cells are differentiated
  • Suitable mammalian sources for oocytes include sheep,
  • the oocytes will be
  • oocytes obtained from ungulates, and most preferably bovine. Methods for isolation of oocytes are well known in
  • bovine oocytes is slaughterhouse materials.
  • This process generally requires collecting
  • prophase I immature oocytes from mammalian ovaries
  • bovine ovaries obtained at a slaughterhouse
  • this period of time is
  • metaphase II oocytes are collected surgically from either
  • human chorionic gonadotropin hCG or similar hormone.
  • stage oocyte as the recipient oocyte because at this stage
  • the oocyte can be or is sufficiently
  • the oocyte activation period generally ranges from
  • immature oocytes may be washed in HEPES
  • HECM buffered hamster embryo culture medium
  • TCM tissue culture medium
  • gonadotropins such as luteinizing hormone (LH) and
  • FSH follicle stimulating hormone
  • the oocytes will preferably be removed and
  • metaphase II oocytes as determined by the presence of
  • Enucleation may be effected by known methods, such as
  • oocytes are either placed in HECM, optionally containing 7.5 micrograms per milliliter cytochalasin B,
  • an embryo culture medium for example an embryo culture medium such as
  • Enucleation may be accomplished microsurgically using
  • the oocytes may then be screened to identify
  • screening may be effected by staining the oocytes with 1
  • culture medium e.g., CRlaa plus 10% serum.
  • the recipient oocytes will be identical to the recipient oocytes.
  • enucleated oocyte will be used to produce NT units
  • Electrofusion is
  • Sendai virus as a fusogenic agent (Graham, lis e Inot .
  • the mammalian cell and oocyte are identical to each other.
  • the mammalian cell and oocyte are identical to each other.
  • suitable medium until activation e.g., CRlaa medium.
  • the NT unit may be activated by known methods. Such as
  • methods include, e.g., culturing the NT unit at sub-
  • activation may be achieved by
  • activation may be effected by
  • the oocyte This will generally be effected by introducing divalent
  • cations into the oocyte cytoplasm e.g., magnesium,
  • strontium barium or calcium, e.g., in the form of an
  • Phosphorylation may be reduced by known methods
  • kinase inhibitors e.g., serine-
  • threonine kinase inhibitors such as 6 -dimethyl -
  • a phosphatase may be inhibited by introduction of a phosphatase into the
  • oocyte e.g., phosphatase 2A and phosphatase 2B.
  • NT activation is effected by
  • the activated NT units may then be cultured in a
  • bovine embryo culture and maintenance include Ham's F-10
  • FCS fetal calf serum
  • oocytes is TCM-199, and 1 to 20% serum supplement
  • medium includes TCM-199 with Earl salts, 10% fetal calf
  • oviduct cells oviduct cells, BRL cells and uterine cells and STO cells.
  • Patent 5,096,822 to Rosenkrans, Jr. et al . which is
  • CR1 contains the nutritional substances necessary
  • CR1 contains hemicalcium L-lactate in amounts ranging
  • Hemicalcium L-lactate is L-lactate with a hemicalcium salt
  • Hemicalcium L-lactate is
  • L-lactate also serves as valuable mineral and energy
  • CR1 medium does not contain serum
  • media comprising animal cells such as oviductal
  • CR1 medium examples include
  • CR1 medium preferably contains the following
  • the activated NT embryos unit are
  • the activated NT units may be any suitable activated NT units.
  • the activated NT units may be any suitable activated NT units.
  • CRlaa medium containing 10% FCS and 6 mg/ml
  • well plates which preferably contain a
  • Suitable confluent feeder layer Suitable confluent feeder layer. Suitable feeder layers
  • fibroblasts include, by way of example, fibroblasts and epithelial cells
  • fibroblasts e.g., fibroblasts and uterine epithelial cells
  • murine e.g., murine
  • the feeder cells comprise mouse
  • the NT units are cultured on the feeder layer until
  • the NT units reach a size suitable for transferring to a
  • NT units will be cultured until at least about 2 to 400
  • the culturing will be effected under suitable conditions, i.e., about 38.5°C and
  • the stage of the NT embryo is in
  • the present invention can also be used to clone
  • the differentiated cells used for donor nuclei have a desired gene inserted, removed or
  • a desired gene from a mammalian cell may be used for
  • the gene may be heterologous. Included is the
  • the present invention can thus be used to provide
  • transgenic or genetically engineered animals and chimeric
  • transgenic and/or chimeric fetuses can be used
  • a feeder layer e.g., irradiated fibroblast
  • the cells used to obtain the stem cells are typically used to obtain the stem cells.
  • portion of the cultured NT unit which is preferably at
  • NT unit may also be used to obtain ES cells and
  • a layer in a suitable growth medium e.g., alpha MEM
  • growth medium is changed as often as necessary to optimize
  • growth e.g., about every 2-3 days.
  • CICM cells or cell lines One skilled in the art can vary
  • transgenic mammalian CICM cells may be produced
  • transgenic NT units can then be used to produce
  • the resultant CICM cells and cell lines preferably
  • cells may be used for cell transplantation therapies.
  • Human CICM cells have application in the treatment of
  • the CICM cells according to the invention will be induced to
  • the subject human CICM human CICM
  • cells may be induced to differentiate into hematopoietic
  • stem cells muscle cells, cardiac muscle cells, liver
  • cartilage cells cartilage cells, epithelial cells, urinary tract
  • hematopoietic stem cells from an embryonic cell line by
  • cell types including hematopoietic cells, muscle, cardiac
  • the subject CICM cells may be used to obtain any
  • human hematopoietic stem cells may be used in medical treatments requiring bone marrow transplantation.
  • late stage cancers such as ovarian cancer and leukemia, as
  • Hematopoietic stem cells can be obtained, e.g.,
  • epithelial cells or lymphocytes with an enucleated
  • Such hematopoietic cells may be used in the
  • a neurological disorder may be fused with an
  • transplantation of such human neural cells include, by transplantation of such human neural cells
  • Parkinson's disease Alzheimer's disease
  • transplanted fetal brain neural cells make the proper connections with surrounding cells and produce dopamine .
  • anti-rejection drugs such as
  • the present invention should eliminate, or at least
  • cell therapy include, by way of example, spinal cord
  • liver diseases i.e., hypercholesterolemia
  • This methodology can be used to replace defective
  • the gene encoding brain derived growth factor may
  • astrocytes have been transfected with
  • retroviral vectors currently used are down-
  • cells include, by way of example, epidermal growth factor,
  • insulin-like growth factor I and II
  • neurotrophin-3 neurotrophin-3/5 , ciliary neurotrophic
  • AFT-1 AFT-1
  • cytokine genes interleukins, interferons, colony stimulating factors, tumor necrosis factors (alpha
  • CICM cells CICM cells, NT fetuses and NT
  • CICM cell fetuses and offspring
  • brain cells across species (xenografts) .
  • xenografts xenografts
  • brain cells For example, brain cells
  • bovine NT fetuses may be used to treat Parkinson's
  • the subject CICM cells preferably human cells
  • CICM cells may be used in drug studies. Further, the subject CICM cells may be used as
  • Fibroblast cells were plated in
  • FCS fetal calf serum
  • the fibroblasts (100 IU/ml) and streptomycin (50 ⁇ l/ml) .
  • tissue was incubated overnight at 10°C in trypsin EDTA
  • FCS fetal calf serum
  • fibroblast cells can be isolated at virtually any time in
  • This procedure can also be used to isolate
  • fibroblasts from other mammals, including mice.
  • a marker gene foreign heterologous DNA
  • embryonic and adult fibroblast cells introduction of a marker gene (foreign heterologous DNA) into embryonic and adult fibroblast cells.
  • the cells were in a single cell suspension. The cells were
  • PBS phosphate buffered saline
  • the reporter gene construct contained the
  • neomycin phosphotransferase fusion gene (beta-GEO) .
  • FCS fetal calf serum
  • G418 400 ⁇ g/ml was added to growth medium for 15

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PCT/US1998/000002 1997-01-10 1998-01-05 Nuclear transfer with differentiated fetal and adult donor cells Ceased WO1998030683A2 (en)

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AU60145/98A AU742363C (en) 1997-01-10 1998-01-05 Nuclear transfer with differentiated fetal and adult donor cells
DE69837270T DE69837270T2 (de) 1997-01-10 1998-01-05 Kerntransfer mit differenzierten fötalen und adulten spenderzellen
IL13082998A IL130829A0 (en) 1997-01-10 1998-01-05 Nuclear transfer with differentiated fetal and adult donor cells
JP53095898A JP2001512964A (ja) 1997-01-10 1998-01-05 分化した胎仔および成体ドナー細胞による核移植
EP98903349A EP1015572B1 (en) 1997-01-10 1998-01-05 Nuclear transfer with differentiated fetal and adult donor cells
CN988027941A CN1248288B (zh) 1997-01-10 1998-01-05 用分化胎儿和成年供体细胞进行的核转移
CA2277192A CA2277192C (en) 1997-01-10 1998-01-05 Nuclear transfer with differentiated fetal and adult donor cells
BR9806872-5A BR9806872A (pt) 1997-01-10 1998-01-05 Transferência nuclear com células diferenciadas doadoras fetais e adultas
NZ336612A NZ336612A (en) 1997-01-10 1998-01-05 Cloning using donor nuclei from proliferating somatic cells
IL130829A IL130829A (en) 1997-01-10 1999-07-07 Nuclear transfer with differentiated fetal and adult donor cells

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US08/781,752 US5945577A (en) 1997-01-10 1997-01-10 Cloning using donor nuclei from proliferating somatic cells

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